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Cell Research 2006年第5期16卷 519-527页

作者： Lan Guo~(1,2,*) Benliang Yin~(1,*) Junli Zhou~(1,3) Xueyong Li~3 Xing Wang Deng~(1,2,3) ~1National Institute of Biological Science,Zhongguancun Life Science Park,Beijing 102206,China ~2Peking-yale Joint Center of Plant molecular Genetics and Agrobiotechnology,College of Life Sciences,Peking university,Beijing 100871,China ~3department of molecular,cellular,and developmental biology,yale university,new haven,connecticut 0652.-8104,usa National Institute of Biological Science Zhongguancun Life Science Park Beijing China Peking-yale Joint Center of Plant molecular Genetics and Agrobiotechnology College of Life Sciences Peking University Beijing China department of molecular Cellular and Developmental Biology Yale University New Haven USA

In addition to DNA sequence information,site-specific histone modifications are another important determinant ofgene expression in a eukaryotic *** selected four modification sites in common histones that are known tosignificantly impact chromatin function and generated monoclonal or polyclonal antibodies that recognize each of thosesite-specific *** used these antibodies to demonstrate that the site-specific histone modification levelsremain relatively constant in different organs of the same *** also compared the levels of selected histonemodifications among several representative organisms and found that site-specific modifications are highly variable amongdifferent organisms,providing new insight into the evolutionary divergence of specific histone modifications.

关键词： histone modification site-specific antibody chromatin

Signaling mechanisms for regulation of chemotaxis

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Cell Research 2005年第1期15卷 52-56页

作者： Dianqing WU* department of Genetics and developmental biology, university of connecticut Health Center, Farmington, CT 06030, usa department of Genetics and developmental biology University of Connecticut Health Center Farmington USA

Chemotaxis is a fascinating biological process, through which a cell migrates along a shallow chemoattractant gradient that is less than 5% difference between the anterior and posterior of the cell. Chemotaxis is composed of two independent, but interrelated processes-motility and directionality, both of which are regulated by extracellular stimuli, chemoattractants. In this mini-review, recent progresses in the understanding of the regulation of leukocyte chemotaxis by chemoattractant signaling are reviewed.

关键词： chemotaxis leukocyte signaling chemoattractant.

Disruption of phytoene desaturase gene results in albino and dwarf phenotypes in Arabidopsis by impairing chlorophyll, carotenoid, and gibberellin biosynthesis

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Cell Research 2007年第5期17卷 471-482页

作者： Genji Qin1 Hongya Gu1,2 Ligeng Ma1,3 Yiben Peng1 Xing Wang Deng1,3 Zhangliang Chen1 Li-Jia Qu1,2 1 Peking-yale Joint Center for Plant molecular Genetics and Agrobiotechnology, National Laboratory for Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking university, Beijing 100871, China 2 The National Plant Gene Research Center (Beijing), Beijing 100101, China 3 department of molecular, cellular, and developmental biology, yale university, new haven connecticut 62520-8104, usa Peking-yale Joint Center for Plant molecular Genetics and Agrobiotechnology National Laboratory for Protein Engineering and Plant Genetic Engineering College of Life Sciences Peking University Beijing China The National Plant Gene Research Center (Beijing) Beijing China department of molecular Cellular and Developmental Biology Yale University New Haven Connecticut USA

Carotenoids play an important role in many physiological processes in plants and the phytoene desaturase gene （PDS3）encodes one of the important enzymes in the carotenoid biosynthesis pathway. Here we report the identification andanalysis of a T-DNA insertion mutant of PDS3 gene. Functional complementation confirmed that both the albino anddwarf phenotypes of the pds3 mutant resulted from functional disruption of the PDS3 gene. Chloroplast development wasarrested at the proplastid stage in the pds3 mutant. Further analysis showed that high level of phytoene was accumulatedin the pds3 mutant. Addition of exogenous GA3could partially rescue the dwarf phenotype, suggesting that the dwarfphenotype of the pds3 mutant might be due to GA deficiency. Microarray and RT-PCR analysis showed that disruptingPDS3 gene resulted in gene expression changes involved in at least 20 metabolic pathways, including the inhibition ofmany genes in carotenoid, chlorophyll, and GA biosynthesis pathways. Our data suggest that the accumulated phytoenein the pds3 mutant might play an important role in certain negative feedbacks to affect gene expression of diverse cel-lular pathways.

关键词： Arabidopsis thaliana phytoene desaturase albino dwarf microarray analysis

Posterior gut development in Drosophila:a model system for identifying genes controlling epithelial morphogenesis

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Cell Research 1998年第4期8卷 273-284页

作者： LENGYEL JUDITH A XUE JUN LIU (department of molecular, Cell and developmental biology university of California at Los Angeles, Los Angeles, CA usa, 90095-1606 usa) department of molecular Cell and Developmental Biology University of California at Los Angeles Los Angeles USA

The posterior gut of the Drosophila embryo, consisting of hindgut and Malpighian tubules, provides a simple,well-defined system where it is possible to use a genetic approach to define components essential for epithelial *** review here the advantages of Drosophila as a model genetic organism, the morphogenesis of the epithelial structures of the posterior gut, and what is known about the genetic requirements to form these *** overview, primordia are patterned by expression of hierarchies of transcription factors; this leads to localized expression of cell signaling molecules, and finally, to the least understood step: modulation of cell adhesion and cell shape. We describe approaches to identify additional genes that are required for morphogenesis of these simple epithelia, particularly those that might play a structural role by affecting cell adhesion and cell shape.

关键词： Organogenesis cell rearrangement convergent extension hindgut Malpighian tubule

Characterization of Function of Three Domains in Dishevelled-1: DEP Domain is Responsible for Membrane Translocation of Dishevelled-1

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Cell Research 2004年第4期14卷 324-330页

作者： Wei Jun PAN, Shu Zhao PANG, Tao HUANG, Hui Yun GUO, Dianqing WU, Lin LI Laboratory of molecular Cell biology, Institute of Biochemistry and Cell biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, 320 Yueyang Rd, Shanghai 200031 College of Life Science, Jilin university, Changchun 130061, China. department of Genetics and developmental biology, university of connecticut Health Center, Farmington, connecticut 06030, usa. Laboratory of molecular Cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences Shanghai College of Life Science Jilin University Changchun China department of Genetics and developmental biology University of Connecticut Health Center Farmington Connecticut USA

Wnt signaling plays an important role in embryogenesis and tumorgenesis. Although the mechanism about how Wnts transduce their signaling from receptor frizzled (Fz) to cytosol has not been understood, dishevelled (Dvl) protein was considered as the intersection of Wnt signal traffic. In this study, we characterized the function of three domains (DIX, PDZ and DEP) of Dvl-1 in canonical Wnt signal transduction and Dvl-1 membrane translocation. It was found both DIX and DEP domain were sufficient to block Wnt-3a-induced LEF-1 transcriptional activity and free cytosol β-catenin accumulation; whereas PDZ domain and a functional mutant form of DEP domain (DEP-KM) had no effect on canonical Wnt signaling. In addition, when cotransfected with Fz-7, DEP domain, but not DIX, PDZ or DEP-KM, translocated and co-localized with Fz-7 to the plasma membrane, which was similar to Dvl-1. Furthermore, it was DEP domain that could block Fz-7-induced membrane translocation of Dvl-1 via a possible competitive mechanism. Thes

关键词： Wnt frizzled-7 dishevelled DIX PDZ DEP membrane translocation signal transduction

Making a tooth:growth factors,transcription factors,and stem cells

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Cell Research 2005年第5期15卷 301-316页

作者： Yan Ding ZHANG1,2, Zhi CHEN1, Yi Qiang SONG3, Chao LIU2, Yi Ping CHEN2,3,* 1College of Stomatology, Wuhan university, Wuhan 430072, China 2College of Bioengineering, Fujian Normal university, Fuzhou 350007, China 3Division of developmental biology, department of Cell and molecular biology, Tulane university, new Orleans, LA 70118, usa College of Stomatology Wuhan University Wuhan China College of Bioengineering Fujian Normal University Fuzhou China Division of developmental biology Department of Cell and Molecular Biology Tulane University New Orleans USA

Mammalian tooth development is largely dependent on sequential and reciprocal epithelial-mesenchymal interactions. These processes involve a series of inductive and permissive interactions that result in the determination, differentiation, and organization of odontogenic tissues. Multiple signaling molecules, including BMPs, FGFs, Shh, and Wnt proteins, have been implicated in mediating these tissue interactions. Transcription factors participate in epithelial-mesenchymal interactions via linking the signaling loops between tissue layers by responding to inductive signals and regulating the expression of other signaling molecules. Adult stem cells are highly plastic and multipotent. These cells including dental pulp stem cells and bone marrow stromal cells could be reprogrammed into odontogenic fate and participated in tooth formation. Recent progress in the studies of molecular basis of tooth development, adult stem cell biology, and regene- ration will provide fundamental knowledge for the realization of human tooth regeneration in the near future.

关键词： tooth development transcription factor growth factor stem cells regeneration.

Differential regulation of cadherin expression by osteotropic hormones and growth factors in vitro in human osteoprogenitor cells

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Acta Pharmacologica Sinica 2005年第6期26卷 705-713页

作者： Peng LIU~(2,3), Jian-hao LIN~(1,3), Bin ZHANG~1 ~1 Arthritis Clinic and Research Center, People's Hospital, Medical Health Center, Peking university, Beijing 100044, China ~2 department of Genetics and developmental biology, university of connecticut Health Center, Farmington, CT 06030, usa department of Genetics and developmental biology University of Connecticut Health Center Farmington USA Arthritis Clinic and Research Center People's Hospital Medical Health Center Peking University Beijing China

Aim: To examine if cadherins are expressed constitutively in human bone marrow stromal cells （hBMSC） and investigate the regulation of cadherin expression by various osteotropic hormones and local factors. Methods: Cadherin expression was examined in first passaged （secondary） hBMSC as well as in the conditionally-immortalized human osteoprogenitor cell line （hOP-7）. Using a monoclonal antibody （MoAb C-1821） to a cytoplasmic domain common to all known cadherins （pan-cadherin MoAb）, cadherins were immunolocalized in first passaged hBMSC as well as in hOP-7 cells. In addition, intense immunostaining for cadherin expression was associated with alkaline phosphatase （ALP） in nodules formed in the high density cultures of hOP-7 cells. Human E-cadherin （HECD） was specifically detected by Western blotting in extracts of untreated hBMSC using an anti-HECD MoAb 004FD. Results: Differential regulation of cadherin expression by various osteotropic hormones and local factors （parathyroid hormone, dexamethasone, estradiol, prostaglandin E2, basic fibroblast growth factor, and tumor necrosis factor-） was also observed. In addition, blocking cadherins with the MoAb C-1821 increased basal ALP activity and had an additive effect on 1, 25（OH）2D3-induced ALP activity. Conclusion: Cadherins are expressed in human osteoprogenitor cells and are involved in the osteogenic differentiation. The differential modulation of cadherin expression by osteotropic agents indicates that these agents may regulate osteoprogenitor cells through different cadherins and these cadherins may play different roles.

关键词： cadherins stromal cells cell differentiation dexamethasone prostaglandin E2 tumor necrosis factor basic fibroblast growth factor estradiol parathyroid hormone

Msx homeobox gene family and craniofacial development

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Cell Research 2003年第6期13卷 429-442页

作者： SYLVIA ALAPPAT Division of developmental biology department of Cell and molecular biology Tulane university new Orleans LA 70118 usa.

Vertebrate Msx genes are unlinked, homeobox-containing genes that bear homology to the Drosophila muscle segment homeobox gene. These genes are expressed at multiple sites of tissue-tissue interactions during vertebrate embryonic development. Inductive interactions mediated by the Msx genes are essential for normal craniofacial, limb and ectodermal organ morphogenesis, and are also essential to survival in mice, as manifested by the pheno typic abnormalities shown in knockout mice and in humans. This review summarizes studies on the expression, regulation, and functional analysis of Msx genes that bear relevance to craniofacial development in humans and mice.

关键词： Msx genes craniofacial tooth cleft palate suture development transcription factor signaling molecule.

Searching for transcriptomic and epigenomic basis of hybrid vigor(heterosis)

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Searching for transcriptomic and epigenomic basis of hybrid ...

Gene silencing:Double-stranded RNA mediated mRNA degradation and gene inactivation

从植物科学到农业发展——2012全国植物生物学大会

作者： Xing Wang Deng and He Hang Peking-yale Joint Center of Plant molecular Genetics and Agrobiotechnology,State Key Laboratory of Protein and Plant Gene Research,School of Life Sciences,Peking university,Beijing 100871,China department of molecular,cellular and developmental biology,yale university,new haven,CT 0652., usa

Chromatin state and genome activity of the hybrid and their differences from that of parental lines are pivotal to understanding the molecular mechanisms of heterosis in plant. In recent studies,we generated genome-wide integrated maps of mRNA and small RNA transcripts,DNA methylation,and representative histone modifications(H3K4me3, H3K9ac and H3K27me3) of Arabidopsis,rice and maize hybrid crosses using high-throughput Illumina sequencing *** found that gene activity was correlated with DNA methylation and both active and repressive histone modifications in transcribed *** epigenetic modifications correlated with changes in transcript levels among hybrids and parental *** compared to gene expression and active histone modifications,repressive epigenetic marks of DNA methylation and H3K27me3 tend to diverge more strongly among hybrid crosses and between organs. Hybrids show highly organ-specific variation in gene expression and epigenetic modifications and exhibit distinct functional enrichments between shoots and roots for differential expressed *** observed a high correlation of allelic bias of epigenetic modifications or gene expression in reciprocal hybrids with their differences in the parental *** genes exhibiting allelic expression bias in hybrids,nucleosome assembly pathway is highly enriched in shoots,while no functional enrichment was observed in roots. We observed simultaneous increase in the number and decrease in the expression levels of smRNA clusters in both organs of hybrids,and found that the increased smRNA expression was accompanied by the enhanced DNA methylation within the same genomic locus in hybrids relative to ***,our data reveal a comprehensive overview of transcriptional and epigenetic trends in hybrid crosses and suggested that hybrid probably evolved divergent genetic and epigenetic regulatory mechanisms for the growth vigor of different organs.

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来源： cnki会议评论

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Cell Research 2001年第3期11卷 181-186页

作者： TANG WEI, XIAO YAN LUO, VANESSA SANMUELS North Carolina State university, Forest Biotechnology Groap, Raleigh, NC 27695, usa university of North Carolina, department of Cell and developmental biology, ChaPel Hill, NC 27599, usa North Carolina State university Forest Biotechnology Group Raleigh USA department of Cell and developmental biology University of North Carolina Chapel Hill USA

The recent development of gene transfer approaches in plants and animals has revealed that transgene can undergo silencing after integration in the genome. Host genes can also be silenced as a consequence of the presence of a homologous transgene. More and more investigations have demonstrated that double- stranded RNA can silence genes by triggering degradation of homologous RNA in the cytoplasm and by directing methylation of homologous nuclear DNA sequences. Analyses of Arabidopsis mutants and plant viral suppressors of silencing are unraveling RNA-silencing mechanisms and are assessing the role of methy- lation in transcriptional and posttranscriptional gene silencing. This review will focus on double-stranded RNA mediated mRNA degradation and gene inactivation in plants.

关键词： Gene silencing double-stranded RNA methylation homologous RNA transgene.