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Dynamics of morphological changes for mitochondrial fission and fusion

Science China(Physics,Mechanics & Astronomy) 2010年第4期53卷 680-689页

作者： WANG ShiQi 1,2,4 , FU ChangLiang 1,2 , ZHANG Yan 1,2 , CHEN Quan 3 & LONG Mian 1,2 1 Key laboratory of Microgravity (National Microgravity laboratory), Beijing 100190, China 2 center for Biomechanics and Bioengineering, Institute of Mechanics, Chinese Academy of Sciences, Beijing 100190, China 3 State Key laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China 4 Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy of Sciences, Beijing 100044, China Key laboratory of Microgravity (National Microgravity laboratory) Beijing China center for Biomechanics and Bioengineering Institute of Mechanics Chinese Academy of Sciences Beijing China Institute of Vertebrate Paleontology and Paleoanthropology Chinese Academy of Sciences Beijing China State Key laboratory of Biomembrane and Membrane Biotechnology Institute of Zoology Chinese Academy of Sciences Beijing China

Mitochondria experience continuous fusion and fission in a living cell, but their dynamics remains poorly quantified. Here a theoretical model was developed, upon a simplified population balance equation (PBE), to predict the morphological changes induced by mitochondrial fission and fusion. Assuming that both fission and fusion events are statistically independent, the survival probability of mitochondria staying in the fission or fusion state was formulated as an exponentially-decayed function with time, which depended on the time-dependent distribution of the mitochondrial volume and the fission and fusion rates. Parametric analysis was done for two typical volume distributions. One was Gamma distribution and the other was Gaussian distribution, derived from the measurements of volume distribution for individual mitochondria in a living cell and purified mitochondria in vitro. The predictions indicated that the survival probability strongly depended on morphological changes of individual mitochondria and was inversely correlated to the fission and fusion rates. This work provided a new insight into quantifying the mitochondrial dynamics via monitoring the evolution of the mitochondrial volume.

关键词： mitochondria morphology fission fusion dynamics modeling

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Evolutionary implications of Avian Infectious Bronchitis Virus(AIBV)analysis

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Cell Research 2006年第3期16卷 323-327页

作者： Peng Shi~(1,2) Li Yu~2 Yun-xin Fu~3 Jing-Fei Huang~1 Ke-Qin Zhang~2 Ya-ping Zhang~(1,2)1 laboratory of Cellular and Molecular Evolution,and Molecular Biology of Domestic Animals,Kunming lnstitute of Zoology,Chinese Academy of Sciences,Kunming 650223,China 2 laboratory for Conservation and Utilization of Bio-resource,Yunnan University,Kunming 650091,China 3 Human Genetics center,University of Texas at Houston,Houston,Texas 77030,USA laboratory of Cellular and Molecular Evolution and Molecular Biology of Domestic Animals Kunming Institute of Zoology Chinese Academy of Sciences Kunming 650223 China.

For developing efficient vaccines,it is essential to identify which amino acid changes are most important to the sur-vival of the *** investigate the amino acid substitution features in the Avian Infectious Bronchitis Virus (AIBV)antigenic domain of a vaccine serotype (DE072) and a virulent viral strain (GA98) to better understand adaptive evo-lution of *** addition,the SARS Coronavirus (SARS-CoV) was also analyzed in the same *** is interestingto find that extreme comparability exists between AIBV and SARS in amino acid substitution *** suggests thatamino acid changes that result in overall shift of residue charge and polarity should be paid special attention to duringthe development of vaccines.

关键词： Avian Infectious Bronchitis Virus SARS Coronavirus positive selection adaptive evolution vaccine development

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CHEMICAL STRUCTURE DEPENDENCE OF THE INTERNAL ENERGY CONTRIBUTION IN RUBBER ELASTICITY

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Chinese Journal of Polymer Science 2001年第5期19卷 447-449页

作者： Juan Sun Xiao-zhen Yang Polymer Physics laboratory center for Molecular Sciences, Institute of Chemistry Chinese Academy of Sciences Beijing 100080 China 中科院化学所高分子物理及化学国家重点实验室北京

Conformational elasticity theory recently developed has been used to explore the internal energy contribution to the elastic force f(e)/f as a function of strain for poly(cis-1,4-isoprene) and poly(trans-1,4-isoprene). Calculated f(e)/f values are in good agreement with those obtained experimentally. Results show that behavior of f(e)/f is mainly contributed by chemical structure, or intramolecular interaction, supporting the experimental observations, and that the internal energy contribution is strain dependent.

关键词： conformational elasticity theory internal energy contribution poly(cis-1,4-isoprene) poly(trans-1,4-isoprene)

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Impact of thickness of GaN buffer layer on properties of AlN/GaN distributed Bragg reflectors grown by metalorganic chemical vapor deposition

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Science China(Technological Sciences) 2010年第2期53卷 313-316页

作者： WU ChaoMin1, SHANG JingZhi1, ZHANG BaoPing1,2, ZHANG JiangYong1,3, YU JinZhong1,3 & WANG QiMing1,31 Department of Physics and Semiconductor Photonics Research center, Xiamen University, Xiamen 361005, China 2 Pen-Tung Sah Micro/Nano Technology Research center, Xiamen University, Xiamen 361005, China 3 State Key laboratory on Integrated Optoelectronics, Institute of Semiconductors, Chinese Academy of Sciences, Beijing 100083, China Department of Physics and Semiconductor Photonics Research center Xiamen University Xiamen China Pen-Tung Sah Micro/Nano Technology Research center Xiamen University Xiamen China State Key laboratory on Integrated Optoelectronics Institute of Semiconductors Chinese Academy of Sciences Beijing China

We studied the impact of the thickness of GaN buffer layer on the properties of distributed Bragg reflector (DBR) grown by metalorganic chemical vapor deposition (MOCVD). The samples were characterized by using metallographic microscope, transmission electron microscope (TEM), atomic force microscopy (AFM), X-ray diffractometer (XRD) and spectrophotometer. The results show that the thickness of the GaN buffer layer can significantly affect the properties of the DBR structure and there is an optimal thickness of the GaN buffer layer. This work would be helpful for the growth of high quality DBR structures.

关键词： MOCVD DBR high-reflectivity nitride

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Interaction effects between estrogen receptor α and vitamin D receptor genes on age at menarche in Chinese women

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Acta Pharmacologica Sinica 2005年第7期26卷 860-864页

作者： Hong XU~(1,2) Ji-rong LONG~3 Miao-xin LI~1 Hong-wen DENG~(1,3) 1 laboratory of Molecular and Statistical Genetics,College of Life Sciences,Hu-nan Normal University,Changsha 410081,China 2 Department of Physiology,Jiangxi Medical College,Nanchang 330006,China 3 Osteoporosis Research center and Department of Biomedical Sciences,Creighton University Medical center,601 N 30th St,Suite 6787,Omaha,NE 68131,USA laboratory of Molecular and Statistical Genetics College of Life Sciences Hu-nan Normal University Changsha China Department of Physiology Jiangxi Medical College Nanchang China Osteoporosis Research center and Department of Biomedical Sciences Creighton University Medical Center Omaha USA

Aim:To evaluate whether estrogen receptor α(ER-α)and vitamin D receptor(VDR)genes are associated with the age at menarche in Chinese ***:A total of 390 pre-menopausal Chinese women were genotyped at the ER-α PvuII,XbaI,and VDR Apal loci using polymerase chain reaction(PCR)-restriction frag-ment length polymorphism(RFLP).Results:Neither the ER-α gene nor the VDRgene individually had significant effects on the age at menarche in our subjects(P>0.10).However,evidence of interaction effects between the two genes wereobserved:with the aa genotype at the VDR ApaI locus,subjects with haplotypePX at the ER-α gene had,on average,6 months later onset of menarche than thenon-carriers(P=0.01).Conclusion:We found that neither the ER-α gene or theVDR gene had a significant association with the age at menarche ***,potential interaction effects between the two genes were observed inChinese women.

关键词： menarche association haplotype estrogen receptor a gene vitamin D receptor gene

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Hybrid method of limit equilibrium and finite element internal force for analysis of arch dam stability against sliding

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Science China(Technological Sciences) 2011年第4期54卷 793-798页

作者： BAO TengFei1,2,3,XU BaoSong1,2,3 & ZHENG XueQing1,2,3 1State Key laboratory of Hydrology-Water Resources and Hydraulic Engineering,Hohai University,Nanjing 210098,China 2National Engineering Research center of Water Resources Efficient Utilization and Engineering Safety,Nanjing 210098,China 3College of Water Conservancy and Hydropower Engineering,Hohai University,Nanjing 210098,China State Key laboratory of Hydrology-Water Resources and Hydraulic Engineering Hohai University Nanjing China National Engineering Research center of Water Resources Efficient Utilization and Engineering Safety Nanjing China College of Water Conservancy and Hydropower Engineering Hohai University Nanjing China

A hybrid method of limit equilibrium and finite element internal force for analysis of arch dam stability against sliding is *** finite element internal force method(FEIFM) is used to provide more accurate thrust forces acting on the faces of a slip body,and the limit equilibrium method(LEM) is employed to evaluate the factor of safety of the slip *** method presented can deal with a slip body with large amount of geometrically complex slip *** addition,compared with the traditional LEM,it can meet the balance condition of the forces in the slip *** example shows that the factor of safety obtained by the method presented agrees well with the theoretical solution.A practical example is also presented to demonstrate the application of the method in the stability analysis of an arch dam *** results from the examples show that the method is promising in analysis of arch dam stability against sliding.

关键词： arch dam stability against sliding limit equilibrium method finite element internal force method

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Novel cyclophilin D inhibitors derived from quinoxaline exhibit highly inhibitory activity against rat mitochondrial swelling and Ca2+ uptake/release

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Acta Pharmacologica Sinica 2005年第10期26卷 1201-1211页

作者： Hong-xia GUO~(2,3), Feng WANG~(2,3), Kun-qian YU~3, Jing CHEN~3, Dong-lu BAI~3, Kai-xian CHEN~3, Xu SHEN~(3,4,5), Hua-liang JIANG~(3,4,5) ~3 Drug Discovery and Design center, State Key laboratory of Drug Research, Shanghai Institute of Materia Medica, Shanghai Institutes for Biologieal Sciences Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 201203, China ~4 School of Pharmacy, East China University of Science and Technology Shanghai 200237, China Drug Discovery and Design center State Key Laboratory of Drug Research Shanghai Institute of Materia Medica Shanghai Institutes for Biological Sciences Graduate School of the Chinese Academy of Sciences Shanghai 201203 China.

Aim: To investigate methods for identifying specific cyclophilin D （CypD） inhibitors derived from quinoxaline, thus developing possible lead compounds toinhibit mitochondrial permeability transition （MPT） pore opening. Methods: Kinetic analysis of the CypD/inhibitor interaction was quantitatively performed byusing surface plasmon resonance （SPR） and fluorescence titration （FT） ***50values of these inhibitors were determined by PPIase inhibition activity ***: All the equilibrium dissociation constants （KD） of the seven compoundsbinding to CypD were below 10 μmol/L. The IC50values were all consistent withthe SPR and FT results. Compounds GW2, 5, 6, and 7 had high inhibition activitiesagainst Ca2+-dependent rat liver mitochondrial swelling and Ca2+uptake/*** GW5 had binding selectivity for CypD over CypA. Conclusion: Theagreement between the measured IC50values and the results of SPR and FT suggests that these methods are appropriate and powerful methods for fyingCypD inhibitors. The compounds we screened using these methods （GW1-7） arereasonable CypD inhibitors. Its potent ability to inhibit mitochondrial swelling and the binding selectivity of GW5 indicates that GW5 could potentially be used for inhibiting MPT pore opening.

关键词： cyclophilin quinoxalines surface plasmon resonance mitochondrial permeability transition: fluorescence titration inhibitor

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Identification and Characterization of glnA Promoter and its Corresponding Trans-regulatory Protein GlnR in the Rifamycin SV Producing Actinomycete,Amycolatopsis mediterranei U32

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Acta Biochimica et Biophysica Sinica 2006年第12期38卷 831-843页

作者： Hao YU~(1&) Wen-Tao PENG~1 Yang LIU~1 Ting WU~2 Yu-Feng YAO~1 Ming-Xue CUI~1 Wei-Hong JIANG~1 Guo-Ping ZHAO~(1,3,4*) 1 laboratory of Molecular Microbiology,Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200032,China 2 Department of Medicinal Chemistry,School of Pharmacy,Fudan University,Shanghai 200032,China 3 State Key laboratory of Genetic Engineering & Department of Microbiology,School of Life Science,Fudan University,Shanghai 200433,China 4 laboratory of Disease and Health Genomics,Chinese National Human Genome center at Shanghai,Shanghai 201203,China laboratory of Molecular Microbiology Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China Department of Medicinal Chemistry School of Pharmacy Fudan University Shanghai 200032 China State Key laboratory of Genetic Engineering & Department of Microbiology School of Life Science Fudan University Shanghai 200433 China laboratory of Disease and Health Genomics Chinese National Human Genome Center at Shanghai Shanghai 201203 China

The genetic requirements for the transcription of glnA,encoding the major glutaminesynthetase in a rifamycin SV-producing Amycolatopsis mediterranei strain,U32,were *** experiments showed that the promoter of U32 glnA （pglnA） was likely to have two transcriptioninitiation sites:P1and P2,located 157 and 45 nucleotides （nt） upstream of the translational start codon,*** mobility shift and DNase I footprinting analyses revealed a 30 bp cis-element locatedat 45 to 75 nt downstream of P1,or 38 to 68 nt upstream of *** sequence of the cis-element displayedhigh similarity to the corresponding regions of pglnA from Streptomyces coelicolor and *** as a reporter gene,the expression levels of U32 pglnA and its deletion derivatives under differentnitrogen-source conditions were analyzed by detecting the catechol dioxygenase activities in ***54,*** J508 and *** FS10 （glnR mutant）.These in vivo studies showed that theactivation of U32 pglnA in *** required GlnR,and its binding to the U32 pglnA was furtherconfirmed by the gel mobility shift *** and heterologous expression of the U32 glnR allowed usto detect the in vitro interaction between the U32 GlnR and the corresponding pglnA *** shown by in vivo glnR inactivation and complementation indicated that GlnR is essential for theactive transcription of glnA in U32.

关键词： glnA promoter cis-element glnR Amycolatopsis mediterranei

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The multi-factor recombination and processes superimposition model for hydrocarbon accumulation:application to the Silurian in the Tarim Basin

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Petroleum Science 2008年第1期5卷 13-19页

作者： Meng Qingyang Pang Xiongqi Gao Jianbo State Key laboratory of Petroleum Resource and Prospecting China University of Petroleum Beijing 102249 China Basin and Reservoir Research center China University of Petroleum Beijing 102249 China Research center CNOOC Beijing 100027 China

The multi-factor recombination and processes superimposition model for hydrocarbon accumulation is put forward in view of the hydrocarbon geological characteristics of multiple episodes of structural evolution, multiple sets of source-reservoir-seal assemblage, multiple cycles of hydrocarbon accumulation and multiple episodes of readjustment and reconstruction in the complex superimposed basins in China. It is a system including theories and methods that can help to predict favorable exploration regions. According to this model, the basic discipline for hydrocarbon generation, evolution and distribution in the superimposed basins can be summarized in multi-factor recombination, processes superimposition, multiple stages of oil filling and latest stage preservation. With the Silurian of the Tarim basin as an example, based on the reconstruction of the evolution history of the four factors （paleo-anticline, source rock, regional cap rock and kinematic equilibrium belt） controlling hydrocarbon accumulation, this model was adopted to predict favorable hydrocarbon accumulation areas and favorable exploration regions following structural destruction in three stages of oil filling, to provide guidance for further exploration ofoil and gas in the Silurian of the Tarim basin.

关键词： Multi-factor recombination processes superimposition Tarim Basin Silurian favorable exploration region prediction

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Biogenic Isoprene Emission Mechanism from ^(13)CO_2 Exposure Experiments

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Chinese Chemical Letters 2002年第9期13卷 897-900页

作者： Min SHAO State Joint Key laboratory of Environmental Simulation and Pollution Control, center for Environmental Sciences, Peking University, Beijing 100871 State Joint Key laboratory of Environmental Simulation and Pollution Control Center for Environmental Sciences Peking University Beijing 100871 China

Biogenic isoprene emissions have been believed to be from only photosynthesis processes in plant. However nocturnal isoprene emission from pine is detected. And by feeding 13CO2 to plants, it is found that both photos... 详细信息

关键词： Emissions volatile organic compounds (VOCs) 13C labeling isoprene.

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