检索结果-南通市图书馆

Antisense-Induced Blockade of GATA-3 Expression Could Inhibit Th2 Excursion of Tumor Cells in vitro and in vivo

在线全文

学校读者我要写书评

暂无评论

Cellular & Molecular immunology 2005年第3期2卷 189-196页

作者： Dongzhu Yao~1 Xiaojun Zhang~1 Haiming Wei~1 Zhigang Tian~(1,2) ~1Institute of immunology,school of life sciences,university of science & technology of china.~2Corresponding to:Dr.Zhigang Tian,school of life sciences,university of science & technology of china.No.{12.,{12. road,Hefei 230027,china. Institute of immunology School of Life Sciences University of Science and Technology of China Hefei 230027 China.

Previous studies have shown that tumor cells predominantly express Th2 type cytokines and transcription factors. GATA-3, as a Th2-specific transcription factor, plays a central role in positive-regulating Th2 development. So whether the expression of GATA-3 in tumor cells has any effect on tumor development is a question of interest. In the present study, we inhibited the expression of GATA-3 in tumor cells through antisense RNA blockade technique, and observed its effects on tumor in vitro and in vivo. Our results showed that antisense GATA-3 treatment could inhibit the expression of TNF-α and Th2 cytokines in tumor cells, and antisense-induced blockade of GATA-3 could also depress tumor growth in tumor-bearing mice. We suggest that the ratio of T-bet/GATA-3 can be evaluated as a more important marker of the status of Thl/Th2 type. And our results might provide some evidence about the molecular regulatory mechanisms in tumor cell development. Cellular ＆ Molecular immunology. 2005 ;2（3 ）： 189-196.

关键词： GATA-3 antisense RNA tumor cell Th1/Th2 cytokine and transcription factor

Syntheses of amphiphilic biodegradable copolymers of poly(ethyl ethylene phosphate) and poly(3-hydroxybutyrate) for drug delivery

在线全文

学校读者我要写书评

暂无评论

science china.Chemistry 2009年第7期52卷 961-968页

作者： CHENG Jing1 & WANG Jun2 1 Department of Polymer science and Engineering, university of science and technology of china, Hefei 230026, china.2 Hefei National Laboratory for Physical sciences at Microscale, school of life sciences, university of science and technology of china, Hefei 230027, china Department of Polymer science and Engineering University of Science and Technology of China Hefei 230026 China Hefei National Laboratory for Physical sciences at Microscale School of Life Sciences University of Science and Technology of China Hefei 230027 China

Biodegradable and amphiphilic triblock copolymers poly(ethyl ethylene phosphate)-poly(3-hydroxybutyrate)-poly(ethyl ethylene phosphate) (PEEP-b-PHB-b-PEEP) have been successfully synthesized through ring-opening polymerization. The structures are confirmed by gel permeation chromatography and NMR analyses. Crystallization investigated by X-ray diffraction reveals that the block copolymer with higher content of poly(ethyl ethylene phosphate) (PEEP) is more amorphous, showing decreased crystallizability. The obtained copolymers self-assemble into biodegradable nanoparticles with a coreshell micellar structure in aqueous solution, verified by the probe-based fluorescence measurements and transmission electronic microscopy (TEM) observation. The hydrophobic poly(3-hydroxybutyrate) (PHB) block serves as the core of the micelles and the micelles are stabilized by the hydrophilic PEEP block. The size and size distribution are related to the compositions of the copolymers. Paclitaxel (PTX) has been encapsulated into the micelles as a model drug and a sustained drug release from the micelles is observed. MTT assay also demonstrates that the block copolymers are biocompatible, rendering these copolymers attractive for drug delivery.

关键词： poly(3-hydroxybutyrate) polyphosphoester drug delivery polymeric nanoparticles

Expression of prolactin receptor and response to prolactin stimulation of human NK cell lines

在线全文

学校读者我要写书评

暂无评论

Cell Research 2004年第1期14卷 67-73页

作者： Rui SUN~(1,2*), Ai Ling LI~(2*), Hai Ming WEI~1, Zhi Gang TIAN~(1,2,**) ~1school of life sciences, university of science and technology of china, Hefei 230027, anhui, china. ~2Shandong Cancer Biotherapy Center, Shandong Academy of Medical science, Jinan 250062, Shandong, china. 2Shandong Cancer Biotherapy Center Shandong Academy of Medical Science Jinan 250062 Shandong China. 2Shandong Cancer Biotherapy Center Shandong Academy of Medical Science Jinan 250062 Shandong China. 1school of life sciences University of Science and Technology of China Hefei 230027 Anhui China. 2Shandong Cancer Biotherapy Center Shandong Academy of Medical Science Jinan 250062 Shandong China.

We have previously shown a critical role of prolactin (PRL) during maturation and anti-tumor effects of murine natural killer (NK) cells in vitro and in vivo. We extended that study by exploring the ability of human NK cell lines (NK-92 and YT cell) to express PRL receptor (PRL-R) and to respond to PRL stimulation in vitro. Both human NK cell lines constitutively expressed PRL-R on membrane and mRNA transcripts, NK-92 cells contained higher level of PRL-R than YT cells, which correlated to the enhanced capacity of the cells to proliferate and to lyse target cells in response to PRL stimulation in the presence of trace amount of IL-2 or IL-15 in vitro. Two differences between IL-2 and IL-15 in functioning on human NK cells were for the first time observed. PRL synergized with IL-15 to improve proliferation of NK cells in a dose-dependent manner without double peak manifesting like IL-2. Although PRL enhanced the cytotoxicity of IL-2 or IL-15 activated NK cells, it exerted the function through up-regulati

关键词： prolactin receptor NK cell interleukin-2 interleukin-15.

A NOTE ON THE CONSISTENCY OF LS ESTIMATES IN LINEAR MODELS

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Chinese Annals of Mathematics,Series B 2001年第4期22卷 471-474页

作者： CHEN XIRU The Graduate school at Beijing, university of science and technology of china, Beijing 100039, china. The Graduate school at Beijing University of Science and Technology of China Beijing 100039 China

It is well known that when the random errors are iid. with finite variance, the week and the strong consiStency of LS estimate of multiple regression coefficients are equivalent. This note, by constructing a counter-example, shows that this equivalence no longer holds true in case that the random errors possess only the r-th moment with 1≤5 T ＜ 2.

关键词： Linear regression model Consistency LS estimate

Bell violation for the thermal states of an XXZ spin chain with Dzialoshiski-Moriya interaction

在线全文

学校读者我要写书评

暂无评论

science china.Physics,Mechanics & Astronomy) 2010年第11期53卷 2074-2079页

作者： MA XiaoSan 1 & WANG AnMin 2 1 school of Electric Engineering and Information,anhui university of technology,Maanshan 243002,china.2 Department of Modern Physics,university of science and technology of china,Hefei 230026,china 1. school of Electric Engineering and Information Anhui University of Technology Maanshan 243002 China2. Department of Modern Physics University of Science and Technology of China Hefei 230026 China

The effect of Dzialoshiski-Moriya (DM) interaction on the violation of Bell inequality for thermal states of interacting qubits via a two-qubit XXZ spin chain is *** results imply that the DM interaction and anisotropy taking a large positive value can enhance the Bell violation and improve the threshold temperatures of *** the comparison between the Bell violation and thermal entanglement,we find that the threshold temperatures of thermal entanglement are higher than those of the Bell *** implies that some states are entangled but the Bell inequality is not violated.

关键词： Bell violation threshold temperatures DM interaction

cDNA cloning,sequence analysis,and recombinant expression of akitonin beta,a C-type lectin-like protein from Agkistrodon acutus

在线全文

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2004年第03期25卷 118-123页

作者： Xiang-dong ZHA1,2,Jing LIU1,Kang-sen XU3 1 school of life science,university of science and technology,Hefei 230026 school of life science,anhui university,2 Hefei 230039 National Institute for Control of Pharmaceuticals and Biological Products,Beijing 100050,{12.3 school of life science University of Science and Technology school of life science Anhui University National Institute for Control of Pharmaceuticals and Biological Products Beijing 100050 China

AIM: To clone the cDNA of a new member of snake venom C-type lectin-like proteins, to study its structure- function relationships and to achieve its recombinant production. METHODS: PCR primers were designed based on the homology and cDNA was amplified by RT-PCR using total RNA from snake venom gland as the template. The PCR products were cloned into the plasmid pGEM-T and sequenced. The deduced protein sequence was analyzed with some bioinformatic programs. A recombinant expression plasmid was constructed using pBAD- TOPO as vector and transformed into *** TOP10 competent cells. RESULTS: A novel cDNA sequence encod- ing akitonin β was found and accepted by GenBank (accession number AF387100). Akitonin β consists of a typical carbohydrate recognition domain (CRD) of C-type lectins, and it is homologous with other snake venom C-type lectin-like proteins. It was predicted to be a platelet antagonist. Upon induction with arabinose rAkitonin β express- ing in E coli was achieved at a high level (superior to 150 mg/L). The recombinant fusion protein exhibited inhibitory activities on rat platelet aggregation in vitro. CONCLUSION: A new member of snake venom C-type lectin-like proteins was discovered and characterized, and an efficient recombinant expression system was estab- lished for its production.

关键词： snake venoms cDNA akitonin C-type lectin-like proteins recombinant proteins structure- activity relationship

Identification and genetic mapping of four novel genes that regulate leaf development in Arabidopsis

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Cell Research 2000年第4期10卷 325-335页

作者： SUN YUE WEI ZHANG FENG LING LI YING LI GUO TIAN LEI LIU HAI HUANG Shanghai Institute of Plant Physiology, Chinese Academy of science, 300 Fenglin road, Shanghai 200032, china.school of life sciences, university of science and technology of china. 1Shanghai Institute of Plant Physiology Chinese Academy of Science 300 Fenglin Road Shanghai 200032 China 1Shanghai Institute of Plant Physiology Chinese Academy of Science 300 Fenglin Road Shanghai 200032 China 1Shanghai Institute of Plant Physiology Chinese Academy of Science 300 Fenglin Road Shanghai 200032 China 2school of life sciences University of Science and Technology of China. P.O.Box 4 Hefei Anhui 230027 China 1Shanghai Institute of Plant Physiology Chinese Academy of Science 300 Fenglin Road Shanghai 200032 China 3Shanghai Research Center of life sciences Chinese Academy of Sciences 320 Yueyang Road Shanghai 200031 China

Molecular and genetic characterizations of mutants have led to a better understanding of many developmental processes in the model system Arabidopsis thaliana. However, the leaf development that is specific to plants has been little studied. With the aim of contributing to the genetic dissection of leaf development, we have performed a large-scare screening for mutants with abnormal leaves. Among a great number of leaf mutants we have generated by T-DNA and transposon tagging and ethylmethae sulfonate (EMS) mutagenesis, four independent mutant lines have been identified and studied genetically. Phenotypes of these mutant lines represent the defects of four novel nuclear genes designated LL1 (LOTUS LEAF 1), LL2 (LOTUS LEAF 2), URO (UPRIGHT ROSETTE), and EIL (ENVIRONT CONDITION INDUCED LESION). The phenotypic analysis indicates that these genes play important roles during leaf development. FOr the further genetic analysis of these genes and the map-based cloning of LL1 and LL2, we have mapped these genes to chromosome regions with an efficient and rapid mapping method.

关键词： Arabidopsis leaf mutant leaf development genetic mappin?

TYPE X MONOTONE SYSTEMS WITH AN ORDER-INCREASING INVARIANT FUNCTION

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Chinese Annals of Mathematics,Series B 1996年第3期17卷 335-342页

作者： JIANG JIFA(Department of Mathematics, The university of science and technology of china, Hefei 230036, china.) Department of Mathematics University of Science and Technology of China Hefei 230036 China

The author considers the asymptotic behavior of solutions of a class of ordinary dmerentialequations x = F(x) in the nonnegative orthant R7. Suppose that F(o) = o, Fi(x1,..., xn) isnondecreasing in xk for all k≠ i and that F possesses an order-increasing invarian *** it is shown that every bounded solution to such a system converges to a single equilibrium.

关键词： Type K monotone system Monotone flow Invariant function Equilibrium Convergence

Transcriptional and posttranscriptional regulation of a signaling pathway in GABAergic D motor neurons of ***

在线全文

学校读者我要写书评

暂无评论

Transcriptional and posttranscriptional regulation of a sign...