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The Inhibitory Effect of Extracts From Fructus lycii and Rhizoma polygonati on in vitro DNA Breakage by Alternariol

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Biomedical and Environmental Sciences 1996年第1期9卷 67-70页

作者： XU DAO-SONG KONG TIAN-QING AND MA JIAN-QUAN (department of Biochemistry, sun yat-sen university of medical Sciences, guangzhou, 510089, Guangdong Province, china) department of Biochemistry Sun Yat-Sen University of Medical Sciences Guangzhou China.

Alternariol caused DNA single-strand breakage. Conversion of the closed circular double-stranded supercoiled DNA (pBR 322) to the nicked circular form and linear form was used to investigate the effect of extracts of some Chinese medical herbs on DNA nicking induced by alternariol. Some substances in the extracts of Rhizoma polygonati (RP) and Fructus lycii (FL) were shown to protect DNA from the attack by *** substance in the RP may bind to plasmid DNA, and this binding reduces the electrophoretic mobility of DNA. These results indicate that substances from FL and RP may be used as DNA protectors. It is possible that they play an important role in preventing cancer.

关键词： DNA The Inhibitory Effect of Extracts From Fructus lycii and Rhizoma polygonati on in vitro DNA Breakage by Alternariol

Measurement of Episcleral Venous Pressure in Normal Chinese People

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眼科学报 2000年第3期16卷 172-175页

作者： Yimin Zhong, Dawei Pengzhongshan Ophthalmic Center, sun yat-sen university of medical Sciences, guangzhou 510060, china zhongshan Ophthalmic Center Sun Yat-sen University of Medical Sciences Guangzhou 510060 China

Purpose: To measure episcleral venous pressure (EVP) in normal Chinese people. Methods: EVP was measured in 110 eyes of 74 subjects distributed in seven age groups from 10 to 80 years using episcleral venomanometer (EV-310). The differences in EVP values were analyzed with age and either ***: EVP values of the 110 eyes appeared as normal distribution. The value x±SD was (9. 11±1. 22) mmHg, and the 95% normal value range was 6. 73 mmHg ~ 11. 50 mmHg. No statistically significant difference of EVP was found in the seven age groups. And there was no statistically significant difference of EVP between the right and left eyes. Conclusions: The value x ?SD of EVP in Chinese normal eyes is 9. 11 mmHg±1.22 mmHg. The normal value of EVP does not vary with age and either eye. Eye Science 2000; 16: 172- 175.

关键词：巩膜外层静脉压巩膜外层静脉检测仪正常值

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Rieger Syndrome

眼科学报 1998年第1期14卷 52-56页

作者： Juan Deng, Ningli Wangzhongshan Ophthalmic Center, sun yat-sen university of medical Sciences, guangzhou 510060, china zhongshan Ophthalmic Center Sun Yat-sen University of Medical Sciences Guangzhou 510060 China

Purpose: To discuss the clinic features of Rieger syndrome, the reasons of making wrong diagnosis , the way of treatment, and the research progress of its molecular characterization and gene mapping of this ***: Two cases of Rieger syndrome which affected a patient and his daughter were studied. Multiple clinical examinations including photography of anterior segment, go-nioscopy and fundus, Humphrey perimetry, A-scan ultrasonography, multiple tonometry in a day and chromosome examination were performed. Most importantly, ultrasonic biomicro-scope(UBM) was first used to show the abnormalities of anterior segment in this syndrome. Results: Gonioscopic examination revealed many mesoderm tissues remained and some parts of the iris adhered to cornea . In addition to hypoplasia of cornea, iris and chamber angle, UBM showed that there was also hypoplasia of ciliary body. The result of the chomosome examination indicated ***: Rieger syndrome is an autosomal-dominated disorder with

关键词：眼病染色体异常分了生物学 Riegr综合症超生波生物显微镜 ubm

An Experimental Study on Homoharringtonine Liposome and Glaucoma Filtration Surgery

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眼科学报 1999年第1期15卷 51-54,50页

作者： Dawei Peng, Kerning Yu, Shujun Zeng, Yongping Li Jianxian Lin, Yuping Wu, Wenbing Zhouzhongshan Ophthalmic Center , sun yat-sen university of medical Sciences , guangzhou 510060 , china department of Pharmacology, sun yat-sen university of medical Sciences , guangzhou 510089, china zhongshan Ophthalmic Center Sun Yat-sen University of Medical Sciences Guangzhou 510060 China

Purpose: To observe the inhibitory action of homoharringtonine liposome during the healing process of wounds in the filtering sitesMethods: posterior sclerectomies were performed in 14 rabbits. Postoperatively one eye of each rabbit received subconjunctival injections of HH liposome and fellow eye received saline injection in a randomized masked ***; Fourteen days after operation the IOP of experimental eyes reduced significantly (P < 0.01) as compared with the controlled eyes, and the number of remaining filtering blebs increased noticeably (P< 0.05). Pathohistological examination revealed that the number of fibroblasts per square micron in the filtering sites and the thickness of the scars in the center of the filtering sites of the experimental eyes were less than those of the controlled eyes. No serious ocular toxic and side effects were *** : This experiment suggests that homoharringtonine liposome can markedly inhibit the scar formation of filtering sites after glaucoma

关键词：滤光手术青光眼脂质体

The spinal CCL2/CCR2 signaling mediates morphine antinociceptive tolerance in rats

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The spinal CCL2/CCR2 signaling mediates morphine antinocicep...

Percutaneous radiofrequency ablation approach through the spleen:initial case report for pancreatic tail gastrinoma

中国生理学会第23届全国会员代表大会暨生理学学术大会

作者： Chun-Mei ZHAO~1,Yu CHEN~2,Fen HU~1,Jian-Qiang FENG~1,Yu CUI~(1,*) 1 department of physiology,zhongshan medical college,sun yat-sen university,guangzhou 510080,china 2 department of Anesthesiology,First Facilitated Hospital,sun yat-sen university,guangzhou 510080,china

＜正＞Background:The chemokine CCL2 and its receptor CCR2 have been suggested to contribute to peripheral and central sensitization in neuropathic ***,whether CCL2/CCR2 signaling pathway mediates the development of morphine antinociceptive tolerance is poorly ***:Our findings showed that intrathecal chronic morphine for 7 days resulted in continuous and significant decrease in morphine analgesia, indicating the development of morphine *** with the behavioral data,western blotting result showed that chronic morphine exposure unregulated the CCL2 expression in neurons of spinal *** addition, double immunofluorescence result revealed that CCL2 expression was localized in neurons,but not in astrocyte or *** uncover the role of CCL2 in morphine tolerance,CCL2 neutralizing antibody or antagonist of CCL2 receptor（CCR2）,RS 102895 was injected intrathecally before each morphine *** found that CCL2 neutralizing antibody or RS102895 significantly blocked the development of morphine antinociceptive tolerance. Furthermore,RS 102895 also attenuated the expression of the phosphorylated p38（p-p38） MAPK in the microglia of spinal ***,blocking the activity of CCL2 or CCR2 with neutralizing antibody or RS 102895 respectively has no effect on morphine analgesia following the development of ***:The present study provided new evidence that the spinal neuronal CCL2/CCR2 played an important role in mediating the development but not maintenance of morphine ***2/CCR2 might be a new target for preventing the development of morphine antinociceptive tolerance.

关键词： morphine tolerance CCL2 CCR2 p38 MAPK

来源： cnki会议评论

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Chinese Journal of Cancer 2010年第9期29卷 836-841页

作者： Pei-Hong Wu1,2, Chang-Chuan Pan1,2, Zi-Lin Huang1,2, Wang Li1,2, Ming Zhao1,2, Zhi-Wei Zhou1,3 1 State Key Laboratory of Oncology in South china, guangzhou, Guangdong 510060, P.R.china 2 department of medical Imaging & Interventional Radiology, sun yat-sen university Cancer Center, guangzhou, Guangdong 510060, P.R.china 3 department of Abdominal Oncology, sun yat-sen university Cancer Center, guangzhou, Guangdong 510060, P.R.china department of medical Imaging& Interventional Radiology Sun Yat-s en University Cancer Center State Key Laboratory of Oncology in South China Guangdong Guangzhou 510060 department of Abdominal Oncology Sun Yat-sen University Cancer Center State Key Laboratory of Oncology in South China Guangdong Guangzhou 510060

Gastrinoma has a low incidence, and the pancreas-originated gastrinoma is *** gastrinoma patients with liver metastases have poor prognosis and short *** treatment to reduce the tumor burden helps to improve symptoms and slows down tumor progression for patients with unresectable *** report a case of pancreatic tail gastrinoma with unresectable liver *** patient received a comprehensive minimally invasive interventional treatment, that is, chemoembolization and radiofrequency ablation for liver metastases, and percutaneous transplenic radiofrequency ablation combined with radioactive 125I seed implantation for pancreatic tail *** patient was followed up for more than 20 months, and showed no clear evidence of tumor *** explored the safety and feasibility of percutaneous transplenic radiofrequency ablation for unresectable pancreatic tail *** transplenic approach allow more indications for minimally invasive therapy and provides a new treatment option not only for patients with unresectable pancreatic tail tumor but also for patients refusing surgery.

关键词：射频消融胃泌素病例报告尾部局部治疗手术切除肿瘤病介入治疗

Activation of PI3-K/Akt pathway for thermal preconditioning to protect cultured cerebellar granule neurons against low potassium-induced apoptosis

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Acta Pharmacologica Sinica 2007年第2期28卷 173-179页

作者： Lin CAO~(2,3) De-xiong CAO~3 Xing-wen SU~2 Li-jun CHEN~2 Ai-lin LIU~2 Wei-jian JIAN~2 Yu-ping WU~2 Peng-xin QIU~2 Guang mei YAN~(2,4)2 department of Pharmacology,zhongshan medical School,sun yat-sen university,guangzhou 510080,china 3 department of Anesthesiology The Second Affiliated Hospital,sun yat-sen university,guangzhou 510120,china departmentofPharmacology ZhongshanMedicalSchoolSunYat-senUniversityGuangzhou510080China departmentofAnesthesiology TheSecondAffiliatedHospitalSunYat-senUniversityGuangzhou510120China

Aim:This study was designed to investigate whether the activation of thephosphatidylinositol 3-kinase(PI3-K)/Akt pathway is required for thermal pre-conditioning to protect rat cerebellar granule neurons(CGN)against apoptosisinduced by low potassium,and to explore the possibility of a link between theupregulated heat shock protein(HSP)70 expression and Akt activation in theacquisition of neuroprotection induced by thermal ***:CGN cultured for 8 d in vitro were switched to 5K medium for 24 h after thermalpreconditioning(TP;43.5 ℃ for 90 min,then 37 ℃ for 1 h).To study the role of thePI3-K/Akt pathway,a PI3-K inhibitor,LY294002(20 μmol/L)was added into thecultures 1 h before TP.3-(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bro-mide(MTT)assay and fluorescein diacetate staining were used to determine *** 33258 staining and agar gel electrophoresis were used to testthe morphological and biological characters of *** blot analysis wasemployed to detect the levels of phospho-Akt,phospho-glycogen synthase ki-nase 3β(GSK3β) Akt,GSK3β,and ***:TP protected CGN againstapoptosis induced by low ***294002 inhibited the neuroprotectiveeffect on CGN induced by *** induced a robust activation of Akt and theinactivation of GSK3β via ***,the activation of the PI3-K/Aktpathway by TP persisted for 24 h in the 5K ***294002(20 μmol/L)failedto inhibit the upregulated HSP70 expression induced by ***:Theactivation of the PI3-K/Akt pathway is required for TP to protect CGN againstapoptosis induced by low potassium,but the neuroprotective effect by Akt acti-vation is not mediated through the downstream induction of HSP70 expression.

关键词： thermal preconditioning heat cerebellum neurons apoptosis LY294002 phospho Akt phospho-GSK3β HSP70

Pluripotent Embryonic Stem Cells Developed into Medulloepithelioma in Nude Mice Eyes

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Eye Science 2002年第1X期18卷 37-44页

作者： Yongping Li , Xiufeng Zhong , Jianhua Yan , Jianxian Lin, Song Tan Xuan Wu, Shulong Li, Guanguang Feng, Yuzhen Yi zhongshan Ophthalmic Center, sun yat-sen university, guangzhou 510060, china department of Pathophysiology, sun yat-sen university, guangzhou zhongshan Ophthalmic Center Sun Yat-sen University Guangzhou 510060 China

Purpose: The pluripotent embryonic stem cells can differentiate into various kinds offormal tissues. There is no previous report on the differentiation of embryonic stem cellin the intraocular environment. In this paper, the authors tried to investigate theintraocular growth character of mice embryonic stem cells in nude ***: Murine embryonic stem cells were cultured and maintained in anundifferentiated state in vitro. They were transplanted into the right eyes of 20 nude miceby microinjection under operating microscope. Animal eye observation, light microscopeand immunohistochemical examinations were ***: Two to three days after transplantation, small pieces of gray-white materialcould be viewed in the vitreous cavity. Between the 15th and 20th day, the gray-whitemass grew into the anterior chamber in 4 nude mice eyes. Then, the mass at the anteriorchamber extended extraocularly. On the 30th day, a remarkable proptosis was observedin two of the four nude mice. In 6 to 45 days, the mice were executed for morphologicalexamination which showed the following typical structures: (1) Undifferentiated cellswith prominent nucleolius. (2) Flexner-Wintersteiner-like rosettes. (3) Medulloepithe-lioma-like structure: the cells were arranged in sheets, cords, tubes, and cysts. (4) Large,spindle-or astrocyte-like cells. (5) Cartilage-like structure. Immunohistochemically, mostof the cells were highly positive in NSE staining and a few cells were moderately positivein GFAP ***: Both animal eye findings and morphologic examinations certificated thatthe transplanted embryonic stem cells could grow in the eyes of nude mice anddifferentiate into intraocular medulloepithelioma.

关键词：胚胎干细胞移植髓上皮瘤眼肿瘤

Laminar-specific synaptic plasticity in mouse visual cortex

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Laminar-specific synaptic plasticity in mouse visual cortex