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Interferon-α enhances sensitivity of human osteosarcoma U2OS cells to doxorubicin by p53-dependent apoptosis

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Acta Pharmacologica Sinica 2007年第11期28卷 1835-1841页

作者： Xiang-wei YUAN~2,Xiao-feng ZHU~(3,5),Xiu-fang HUANG~4,Pu-yi SHENG~2,Ai-shan HE~2,Zi-bo YANG~2,Rong DENG~3,Gong-kanFENG~3,Wei-ming LIAO~(2,5)~2 department of Orthopedic Surgery,The First Affiliated Hospital,sun yat-sen university,guangzhou 510080,china ~3 State Key Laboratoryof Oncology in South china,Cancer Center,sun yat-sen university,guangzhou 510060,china ~4 department of Pathology,The AffiliatedJiangmen Hospital,sun yat-sen university,Jiangmen 529030,china department of Orthopedic Surgery The First Affiliated Hospital Sun Yat-sen University Guangzhou 510080 China State Key Laboratory of Oncology in South china Cancer Center Sun Yat-sen University Guangzhou 510060 China department of Pathology The Affiliated Jiangmen Hospital Sun Yat-sen University Jiangmen 529030 China

Aim:To determine whether interferon-α(IFNα) can enhance doxorubicinsensitivity in osteosarcoma cells and its molecular ***:Cellviability was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide *** was studied using Flow cytometry analysis,Hoechst33258 staining,DNA fragmentation assay,as well as the activation ofcaspase-3 and poly (ADP-ribose) *** expression was detectedby Western *** dependence of p53 was determined using ***:IFNα increased doxorubicin-induced cytotoxicity to a muchgreater degree through apoptosis in human osteosarcoma p53-wild U2OS cells,but not p53-mutant MG63 ***α markedly upregulated p53,Bax,Mdm2,andp21,downregulated Bcl-2,and activated caspase-3 and PARP cleavage in re-sponse to doxorubicin in U2OS ***,the siRNA-mediated silencing ofp53 significantly reduced the IFNα/doxorubicin combination-induced cytotoxic-ity and PARP ***:IFNα enhances the sensitivity of humanosteosarcoma U2OS cells to doxorubicin by p53-dependent *** propercombination with IFNα and conventional chemotherapeutic agents may be a ra-tional strategy for improving the treatment of osteosarcoma with functional p53.

关键词： Interferon-α doxorubicin apoptosis p53 osteosarcoma

Thermal preconditioning protected cerebellar granule neurons of rats by modulating HSP70 expression

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Acta Pharmacologica Sinica 2004年第4期25卷 60-63页

作者： Li-jun CHEN, Xing-wen SU, Peng-xin QIU, Yi-junHUANG, Guang-mei YAN2 department of Pharmacology, zhongshan medicalcollege, sun yat-sen university, guangzhou 510089, china department of Pharmacology Zhongshan Medical College Sun YatSen University Guangzhou 510089

AIM: To explore the possibility that expressions of different heat shock proteins (HSPs) are specifically involved in the protection of thermal preconditioning against apoptosis of cerebellar granule neurons (CGNs) induced by repolarization. METHODS: Western blotting was used to detect expressions of HSP27, HSP70, and HSP90 in- duced by thermal preconditioning (TP) in CGNs; reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expression level of HSP70 mRNA; apoptosis of CGNs was induced by switching culture medium containing KCl 25 mmol/L to one containing KCl 5 mmol/L. RESULTS: No expression of HSP27 in cerebellar granule neurons was observed with TP at 44 oC. Expression of HSP90 was obvious in CGNs both without and with TP at 44 oC for different periods. Expression of HSP70 protein in CGNs was lower with TP at 44 oC for 5 min, but it increased gradually after the period was prolonged to 30, 60, or 90 min. HSP70 mRNA was detected after TP 44 oC for 30, 60, and 90 min and increased gradually with time. HSP70 antisense oligodeoxynucleotides 10 μmol/Lfor 72 h inhibited the protective effects of TP at 44 oC on apoptosis of CGNs induced by repolarization. CONCLUSION: HSP70 is involved in protective effects of thermal preconditioning on apoptosis in cerebellar granule neurons induced by repolarization.

关键词： thermal preconditioning heat heat shock proteins cerebellum neurons apoptosis antisense oligodeoxynucleotides Western blotting

Biorthogonal multiple wavelets generated by vector refinement equation

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Science china Mathematics 2007年第7期50卷 1015-1025页

作者： Song LI~(1+) Jun XIAN~2 1 department of Mathematics,Zhejiang university,Hangzhou 310027,china 2 department of Mathematics,sun yat-sen university,guangzhou 510275,china 1. department of Mathematics Zhejiang University Hangzhou 310027 China 2. department of Mathematics Sun Yat-Sen University Guangzhou 510275 China

Biorthogonal multiple wavelets are generated from refinable function vectors by using the multiresolution analysis. In this paper we provide a general method for the construction of compactly supported biorthogonal multiple wavelets by refinable function vectors which are the solutions of vector refinement equations of the form $$\varphi (x) = \sum\limits_{\alpha \in \mathbb{Z}^s } {a(\alpha )\varphi (Mx - \alpha ), x \in \mathbb{R}^s } ,$$ where the vector of functions ? = (? 1, …, ? r)T is in $(L_2 (\mathbb{R}^s ))^r ,a = :(a(\alpha ))_{\alpha \in \mathbb{Z}^s } $ is a finitely supported sequence of r × r matrices called the refinement mask, and M is an s × s integer matrix such that lim n→∞ M ?n = 0. Our characterizations are in the general setting and the main results of this paper are the real extensions of some known results.

关键词： refinement equation biorthogonal multiple wavelets refinable function vector 42C40 41A10 41A15 41A25

OCULO-CEREBRO-RENAL SYNDROME (LOWE'S SYNDROME)

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眼科学报 1991年第1期7卷 40-42,46页

作者： Youzhao Chen zhongshan Ophthalmic Center sun yat-sen university of medical Sciences guangzhou, china Guoxiang Pang department of Ophthalmology Peking Union medical college Hospital Beijing, china zhongshan Ophthalmic Center Sun Yat-sen University of Medical Sciences Guangzhou China.

Oculo-cerebro-renal syndrome (Lowe's syndrome) is characterized by mental and motor retardation, cataract, glaucoma and renal abnormalities. It is an X-linked recessive metabolic disease. Two brothers suffering from Lowe's syndrome are reported. Their mother with lenticular opacities and peculiar facial appearance is in concordance with the obligate carrier. The ocular changes and heridity are discussed.

关键词： oculo-cerebro-renal syndrome Lowe’s syndrome congenital cataract carrier female X-R heredity metabolic abnomalities.

Experimental study of gene therapy with angiostatin gene in pancreatic cancer

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Hepatobiliary & Pancreatic Diseases International 2002年第3期1卷 452-457页

作者： Sheng-Li Nie Shi-Zhen Yuan the department of Gastroenterology, sun yat-sen Memorial Hospital, medical college, sun yat-sen university, guangzhou 510120, china department of Gastroenterology Sun Yat-Sen Memorial Hospital Medical College Sun Yat-Sen University Guangzhou 510120 China. victorynie@***

Objective: To study the effects of angiostatin (AS) gene mediated by liposome on human pancreatic cancer cell line SW1990. Methods: Angiostatin gene was cloned into the eu- karyotic expression vector pRC/CMV. The recombi- nant of pRC/CMV-AS was introduced into the pan- creatic cancer cell line, SW1990. The mechanism of anti-tumor was studied and tested. Results: The eukaryotic expression vector pRC/ CMV-AS was identified by the restriction digest. pRC/CMV-AS was stably integrated into the target cells and expressed by Western blot and drug-sensi- tivity tests, and inhibited the vascular endothelial cells proliferation in vitro. In addition, the effects of the angiostatin vector on reducing the volume of tumors implanted in nude mouse models were also noted. Conclusion: This study demonstrated that the recom- binant pRC/CMV-AS mediated by liposome may play a potential role in the treatment of pancreatic cancer in the future.

关键词： angiostatin gene therapy pancreatic cancer vector angiogenesis liposome antiangiogenesis dormancy therapy

IMPROVED DEM-CFD MODEL AND VALIDATION:A CONICAL-BASE SPOUTED BED SIMULATION STUDY

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Journal of Hydrodynamics 2010年第3期22卷 351-359页

作者： RONG Liang-wan ZHAN Jie-min department of Applied Mechanics and Engineering Sun Yat-sen University Guangzhou 510275 China department of Applied Mechanics and Engineering Sun Yat-sen University Guangzhou 510275 China Guangdong Province Key Laboratory of Coastal Ocean Engineering Sun Yat-sen University Guangzhou 510275China

An improved and efficient DEM-CFD approach is developed for spouted beds. A nonlinear Discrete Element Method （DEM）, with a concept of spring, dash-pot and friction slider, is used for tracing the movement of each individual particle. The gas flow is described by a set of reorganized governing equations. Two phases are coupled through contributions due to effects of porosity, viscosity and drag. All equations are solved with the commercial package Fluent with an implementation of User Defined Functions （UDF）. To validate the improved model, a two-dimensional conical-base spouted bed is chosen as a case study. An unstructured mesh system is adopted instead of regular grid system. The simulation also takes the Saffman force and Magnus effect into account. The calculation results show good agreement with the experimental observations which are taken from the literature.

关键词： Discrete Element Method (DEM) spouted bed Fluent User Defined Functions (UDF) unstructured mesh

Muscarinic Receptor Agonists Protect Cultured Bovine Trabecular Meshwork Cells against Apoptosis Induced by Dexamethasone

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眼科学报 2004年第1期20卷 42-47页

作者： Yajuan Gu1,Shujun Zeng2,PengXin Qiu2,Yuping Wu2,Dawei Peng2,Guangmei Yan11department of Pharmacology, zhongshan medical college, sun yat-sen university, guangzhou 510089, china2zhongshan Ophthalmic Center, sun yat-sen university, guangzhou 510060, china department of Pharmacology Zhongshan Medical College Sun Yat-sen University Guangzhou 510089 China.

Purpose:To study whether muscarinic receptor agonists can protect cultured bovine trabecular meshwork cells against apoptosis induced by ***:The third to fifth passags of bovine trabecular meshwork cells were grown to confluence and incubated for 1～14 days in growth media with dexamethasone or pretreatment of pilocarpine or *** cultures were evaluated for apoptosis by phase-contrast microscopy, fluorescence microscopy, DNA laddering and flow cytometric ***:Dexamethasone (0.24～0.96 mmol·L-1) induced apoptosis of trabecular mes-hwork cells in a dose and time-dependent *** 0.48 mmol·L-1 dexametha-sone-treatment, 1.84 mmol·L-1 of pilocarpine or 2.74 mmol·L-1 of carbachol added could significantly reduce apoptotic ***:Muscarinic receptor agonists can protect cultured bovine trabecular meshwork cells against apoptosis induced by dexamethasone. Eye Science 2004;20:42-47.

关键词： apoptosis dexamethasone pilocarpine carbachol trabecular meshwork glaucoma

Reflection of plane electromagnetic waves from the surface of a perfect conductor moving in an arbitrary direction

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Chinese Science Bulletin 2000年第17期45卷 1564-1569页

作者： HE Qicai & HUANG Yaoxiong1. department of Physics, sun yat-sen university of medical Sciences, guangzhou 510089, china 2. Institute of Biomedical Engineering, Ji’nan university, guangzhou 510632, china Correspondence should be addressed to Huang Yaoxiong department of Physics Sun Yat-sen University of Medical Sciences Guangzhou China Institute of Biomedical Engineering Ji’nan University Guangzhou China

The reflection of plane electromagnetic waves (TE wave and TM wave) from a perfect conductor which moves in an arbitrary direction is investigated. Based on Maxwell’s equations and the boundary conditions for moving boundary, the relation between the field vectors of reflected and incident waves, and the reflection coefficient are derived. The energy balance between incident and reflected waves, the force exerted by electromagnetic waves to the moving conductor are also discussed and some new conclusions are suggested for notice.

关键词： plane electromagnetic wave moving conductor reflection coefficient radiation pressure.

Effects　of　He－Ne　laser　irradiation　at　acupuncture　points　on　steroid　hormone　receptors　of　uteri　in　rabbits

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Chinese Journal of Lasers 1994年第4期B3卷 369-375页

作者： HUANG Ping CAI Shangda QIU Yiguang HUANG Wenxin (department of Biology, department of Biochemistry,sun yat-sen university of medical Sciences, guangzhou 510089, china) zhongshan Univ of medical Sciences Guangzhou China

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关键词： He-Ne laser acupoint hormone receptor