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Roles of Salicylic Acid-responsive Cis-acting Elements and W-boxes in Salicylic Acid Induction of VCH3 Promoter in Transgenic Tobaccos

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Acta Biochimica et Biophysica Sinica 2006年第1期38卷 46-52页

作者： Hai-Yan LI~(1,2),Wei WEI~(2&),and Yu LI~(1*) ~1 Institute of Mycology,jilin agricultural university,changchun 130118,china ~2 college of biotechnology,jilin agricultural university,changchun 130118,china Institute of Mycology Jilin Agricultural University Changchun 130118 China college of biotechnology Jilin Agricultural University Changchun 130118 China

A salicylic acid (SA)-inducible VCH3 promoter was recently identified from grapevine (Vitisarnurensis) that contains two inverse SA-responsive cis-acting elements and four *** furtherdemonstrate the roles of these elements,four fragments with lengths from-1187,-892,-589,-276 to+7 bp were fused with the β-glucuronidase (GUS) reporter géne and transferred to Nicotiana tobacum,together with another four VCH3 promoter fragments with mutation in the two inverse *** functions,of each promoter fragment were-examined by analysis of GUS activity in thetransgenic tobacco root treated with *** GUS activity was shown in the roots of transgenictobaccos with the VCH3 (-1187)-GUS construct containing two SA-responsive cis-acting elements andfour ***,GUS activity directed by the VCH3 (-892)-GUS construct,containing one SA cis-acting element and four W-boxes,was reduced by up to 35% compared with that in tobaccos transformedwith the VCH3 (-1187)-GUS construct,indicating that the SA cis-acting element plays an important role inSA induction of the VCH3 *** the m2VCH3 (-1187)-GUS nor the mVCH3 (-892)-GUSconstruct,with mutation on the SA-responsive elements,abolished the expression of GUS activity,demon-strating that the W-boxes in the VCH3 promoter are also involved in SA *** arialysis ofGUS activity directed by each of the eight VCH3 promoter fragments showed that GUS was expressedspecifically in vascular *** was concluded that both the SA-responsive cis-acting elements and the W-boxes are important for the SA induction of the VCH3 *** promoter might have a potential usein plant genetic engineering.

关键词： VCH3 promoter salicylic acid(SA)-responsive cis-acting element W-box site-directed mutagenesis SA induction transgenic tobacco

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牛分枝杆菌MPB63基因的克隆及其在大肠杆菌中的表达

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生物化学与生物物理进展 2005年第2期32卷 129-132页

作者：姜秀云王春凤王春芳何昭阳吉林农业大学生物技术学院吉林农业大学动物科技学院长春130118 吉林农业大学动物科技学院

以牛分枝杆菌Vallee111染色体DNA为模板,以MPB63成熟蛋白基因特异性引物进行PCR扩增,获得约400 bp的DNA片段. 通过T-A克隆技术,将PCR产物克隆至pGEM-T Vector中,成功地构建出克隆载体pGEM-T-63. 以BamHⅠ和EcoRⅠ双酶切pGEM-T-63和pET28... 详细信息

以牛分枝杆菌Vallee111染色体DNA为模板,以MPB63成熟蛋白基因特异性引物进行PCR扩增,获得约400 bp的DNA片段. 通过T-A克隆技术,将PCR产物克隆至pGEM-T Vector中,成功地构建出克隆载体pGEM-T-63. 以BamHⅠ和EcoRⅠ双酶切pGEM-T-63和pET28a(+),并将纯化的MPB63基因亚克隆至pET28a (+)中,构建出原核表达载体pET28a-63. 将pET28a-63转化至感受态***21(DE3)中,经IPTG诱导和SDS-聚丙烯酰胺凝胶电泳分析,可见约18 ku外源蛋白带. 蛋白质印迹分析发现,该蛋白质具有牛分枝杆菌抗原性,从而为进一步研究MPB63的亚单位疫苗及DNA疫苗奠定基础.

关键词：牛分枝杆菌 MPB63基因克隆原核表达

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STRATEGY OF MAIZE′S CONCENTRATING TO ADVANTAGE AREAS IN jilin PROVINCE

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Chinese Geographical Science 2003年第4期13卷 378-382页

作者： JIANG Hui-ming,GU Li-li(Managerial college,jilin agricultural university,changchun 130118,P. R. china) Managerial college Jilin Agricultural University Changchun P. R. China

jilin Province is one of the main grain-producing provinces of china,which has dominant position in maize production,by the view of its advantages in policy,location,breed and market. And after entering WTO,some measures have been taken to enhance maize competitive ability. But there are some difficulties in concentrating production to maize advantaged areas. This paper expounds the basis that jilin Province becomes the advantage area of maize,analyzes the problems and puts forward the supporting policy. Some strategic measures are proposed,as developing comparable advantages,carrying out the strategy of un-equilibrium development and cultivating advantaged product areas of maize to rapidly improve the international competitive ability and productivity of maize in jilin Province,cast the agricultural predicament off and promote the agricultural development into a new stage.

关键词： maize advantage area concentration jilin province

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BAF Complex Is Closely Related to and Interacts with NF1/CTF and RNA Polymerase Ⅱ in Gene Transcriptional Activation

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Acta Biochimica et Biophysica Sinica 2005年第7期37卷 440-446页

作者： Li-Hui ZHAO~(1,2) Xue-Qing BA~1 Xiao-Guang WANG~3 Xiao-Juan ZHU~1 Li WANG~1 Xian-Lu ZENG~1 1 Institute of Genetics and Cytology,Northeast Normal university,changchun 130024,china 2 college of Agronomy,jilin agricultural university,changchun 130118,china 3 Department of Biology,changchun Normal college,changchun 130032,china Institute of Genetics and Cytology Northeast Normal UniversityChangchun 130024 China college of Agronomy Jilin Agricultural UniversityChangchun 130118 China Department of Biology Changchun Normal CollegeChangchun 130032 China

Brg-or hBrm-associated factor (BAF) complexes,a chromatin-remodeling complex family ofmammalian cells,facilitate transcriptional activity by remodeling nucleosome ***1 is the coresubunit of Brg-associated factor *** the present study,we investigated the spatial relationshipbetween Brg1 and nuclear factor 1 (NF1/CTF) and RNA polymerase Ⅱ (RNAP Ⅱ) upon gene transcriptionalactivation in vivo by employing immuno-gold *** data showed that Brg1 was closely co-localizedwith NF1/CTF and RNAP Ⅱ in HeLa ***,the co-immunoprecipitation assay further revealedthat Brg1 can be isolated together with NF1/CTF and RNAP Ⅱ in the ConA-stimulated,but not the resting,T *** combined results suggested that BAF complexes can interact with NF1/CTF and RNAPⅡ,and this interaction is closely dependent on the activation of gene transcription.

关键词： BAF complex NF1/CTF RNAP Ⅱ gene transcription

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Arnold's theorem on properly degenerate systems with the Russmann nondegeneracy

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Science china Mathematics 2005年第12期48卷 1656-1669页

作者： HAN Yuecai & LI Yong college of Mathematics, jilin university, changchun 130012, china college of Mathematics Jilin University Changchun China

In this paper, we investigate the persistence of invariant tori for the nearly in-tegrable Hamiltonian system H(x,y) = h(y) + εp(x,y) + ε2Q(x,y), where h(y) and εp(x,y) satisfy the Russmann non-degenerate condition. Mainly we overcome the difficulties that the order of the parameter E in the perturbation ε2Q(x,y) is not enough and that the measure estimate involves in parts of frequencies with small parameter.

关键词： Arnold’s theorem the Russmann non-degenerate condition.

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A software cascading faults model

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Science china(Information Sciences) 2011年第11期54卷 2454-2458页

作者： LIU YanHeng 1,2,LIU XueLian 3 & WANG Jian 1,2 1 Key Laboratory of Symbolic Computation and Knowledge Engineering of Ministry of Education,jilin university,changchun 130012,china 2 college of Computer Science and Technology,jilin university,changchun 130012,china 3 college of Software,jilin university,changchun 130012,china Key Laboratory of Symbolic Computation and Knowledge Engineering of Ministry of Education Jilin University Changchun China college of Computer Science and Technology Jilin University Changchun China college of Software Jilin University Changchun China

Functions and call relations are extracted into nodes and edges,respectively,by which a novel topological model is *** directional edges and the corresponding weight values express the call relations and tightness *** introducing two concepts of function fault-tolerant capability and software fault intensity and by designing a allocation rule on fault-tolerant capability,a cascading fault model is built to explore fault propagation *** on practical software networks show that a weak fault intensity,a small number of initial faults,and a strong fault-tolerant capability can slow down the spreading *** functions with more call relations and more closer tightness contribute more to the stability of the whole system.

关键词： software fault tolerant cascading faults fault-tolerant capability fault intensity

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Mitochondrial DNA analysis of ancient Sampula population in Xinjiang

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Progress in Natural Science:Materials International 2007年第8期17卷 927-933页

作者： Xie Chengzhi~(1,2) Li Chunxiang~2 Cui Yinqiu~(1,2) Cai Dawei~(1,2) Wang Haijing~2 Zhu Hong~1 Zhou Hui~(1,2**) (1.Research Center for Chinese Frontier Archaeology,jilin university,changchun 130012,china 2.college of Life Science,jilin university,changchun 130023,china) Research Center for Chinese Frontier Archaeology Jilin University Changchun 130012 China college of Life Science Jilin University Changchun 130023 China

The archaeological site of Sampula cemetery was located about 14 km to the southwest of the Luo County in Xinjiang Khotan,china,belonging to the ancient Yutian kingdom.14C analysis showed that this cemetery was used from 217 *** 283 A.D. Ancient DNA was analyzed by 364 bp of the mitochondrial DNA hypervariable region I （mtDNA HVR-I）,and by six restriction fragment length polymorphism （RFLP） sites of mtDNA coding *** successfully extracted and sequenced intact stretches of maternally inher- ited mtDNA from 13 out of 16 ancient Sampula *** analysis of mtDNA haplogroup distribution showed that the ancient Sampula was a complex population with both European and Asian *** joining network of U3 sub-haplogroup and multi-dimen- sional scaling analysis all showed that the ancient Sampula had maternal relationship with Ossetian and Iranian.

关键词： ancient DNA mitochondrial DNA Sampula ancient populations

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In situ preparation of Nanoparticles/polymer composites

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Science china(Technological Sciences) 2008年第11期51卷 1886-1901页

作者： SUN HaiZhu & YANG Bai State Key Lab of Supramolecular Structure and Materials, college of Chemistry, jilin university, changchun 130012, china college of Chemistry, Northeast Normal university, changchun 130024, china State Key Lab of Supramolecular Structure and Materials College of Chemistry Jilin University Changchun China college of Chemistry Northeast Normal University Changchun China

Nanoparticle (NP) is the matter between molecule and bulk material. It has attracted much attention in catalysis, optoelectronics and biology due to its unique physical and chemical properties. Incorporation of these NPs into the polymer matrix is one of the best methods to display their special functions, which not only stabilize the NPs but also realize the functional assembly of NPs and polymers. However, reali- zation of this idea depends largely on the compatibility of NPs and polymers as well as the interaction between them. Therefore, many methods have been developed to prepare the composites of NPs and polymers in order to obtain the function ex- pected. In this review, we mainly focus on the combination of in situ method with other methods to synthesize different functional one-dimension, two-dimension as well as bulk composites, which has been recently developed by our group. The most striking character of our method is the excellent compatibility between NPs and polymers which ensures a homogeneous distribution of NPs in the polymer matrix. The existence of the polymer network makes the NPs more stable, and is significant for displaying their functions.

关键词： in situ nanoparticle polymer composite function

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Properties of meso-tetrakis (4-n-alkanoyloxyphenyl) porphyrin liquid crystal Co and Ni complexes

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Science china Chemistry 2007年第4期50卷 488-493页

作者： LIU Wei1,2 & SHI TongShun2 1 college of Materials Science and Engineering, Shandong university of Technology, Zibo 255049, china 2 Department of Chemistry, jilin university, changchun 130023, china college of Materials Science and Engineering Shandong University of Technology Zibo China Department of Chemistry Jilin University Changchun China

Six series of meso-tetrakis (4-n-alkanoyloxyphenyl) porphyrin Co and Ni complexes (12 kinds) were reported. Nine of the compounds were found to exhibit liquid crystal properties and display a hexagonal columnar discotic columnar (Colh) phase. Molecular structure of all synthesized compounds was confirmed by IR, UV, MS, 1H NIVR, and elemental analysis. These liquid crystalline compounds have been studied by cyclic voltammetry, luminescence, and surface photovoltage spectroscopy.

关键词： porphyrin liquid crystal cyclic voltammetry luminescence surface photovoltage spectroscopy

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Integrative multi-omics approaches reveal that Asian cultivated rice domestication influences its symbiotic relationship with arbuscular mycorrhizal fungi

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Pedosphere 2024年第2期34卷 315-327页

作者： Lei TIAN jilin WANG Hongping CHEN Weiqiang LI Lam-Son Phan TRAN Chunjie TIAN Key Laboratory of Mollisols Agroecology State Key Laboratory of Black Soils Conservation and UtilizationNortheast Institute of Geography and AgroecologyChinese Academy of SciencesChangchun 130102China Rice Research Institute Jiangxi Academy of Agricultural SciencesNanchang 330200China Institute of Genomics for Crop Abiotic Stress Tolerance Department of Plant and Soil ScienceTexas Tech UniversityLubbock TX 79409USA

Potential changes in the symbiotic relationship between rice(Oryza sativa)and microorganisms have occurred during the domestication of Asian cultivated rice(***)from common wild rice(Oryza rufipogon)and in response to global climate change,along with evolving adaptations to the *** potential genes may express differently or dominate the symbiotic relationships between arbuscular mycorrhizal fungi(AMF)and plants,which may be beneficial to rice *** date,research on this important topic has been *** this study,we aimed to examine the symbiotic relationships of Asian common wild and cultivated rice species with *** conducting a comparative metagenomic analysis of the rhizospheres of wild and cultivated rice species,we identified differences in Rhizophagus intraradices-related genes associated with wild and cultivated rice,as well as functional genes of ***,we obtained root-related genes associated with AMF from transcriptome data of rice *** results collectively suggest that ***-related genes in the rhizosphere of wild rice may be more conducive to its ***,bacteria from the Nitrosomonadaceae and Nitrospiraceae families identified in the rhizosphere of wild rice exhibited positive correlations with ***-related genes with protein identifiers 1480749 and1871253,which may indicate that nitrobacteria can enhance the functions of *** in association with wild ***,in a case study using comparative transcriptome analysis of root samples obtained from ***-inoculated wild and cultivated rice plants,we found significantly higher expression levels of the strigolactone pathway-related genes DWARF3(D3)and DWARF14(D14)in ***-inoculated common wild rice than in ***-inoculated cultivated *** study provides a theoretical basis for identifying the effects of domestication on mycorrhizal symbiosis-related genes,which could be promoted in wi

关键词： Rhizophagus intraradices root-related gene symbiotic effect strigolactone wild rice

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