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Transiently Expressed Short Hairpin RNA Targeting 126 kDa Protein of Tobacco Mosaic Virus Interferes with Virus Infection

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Acta Biochimica et Biophysica Sinica 2006年第1期38卷 22-28页

作者： Ming-Min ZHAO~(1,2),De-Rong AN~(1*),Jian ZHAO~2,Guang-Hua HUANG~3,Zu-Hua HE~4,and Jiang-Ye CHEN~3 ~1 college of Plant Protection,Northwest Science and Technology university of agriculture and Forestry,Yangling 712100,china ~2 college of agriculture,yangtze university,jingzhou 434025,china ~3 State Key Laboratory of Molecular Biology,Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences,Shanghai 200031,china ~4 State Key Laboratory of Plant Molecular Genetics,Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences,Shanghai 200032,china college of Plant Protection Northwest Science and Technology University of Agriculture and Forestry Yang ling 712100 China college of agriculture Yangtze University Jingzhou 434025 China State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China State Key Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China

RNA-interference (RNAi) silences gene expression by'guiding mRNA degradation in asequence-specific *** interfering RNA (siRNA),an intermediate of the RNAi pathway,has beenshown to be very effective in inhibiting virus infection in mammalian cells and cultured plant ***,wereport that Agrobacterium tumefaciens-mediated transient expression of short hairpin RNA (shRNA) couldinhibit tobacco mosaic virus (TMV) RNA accumulation by targeting the gene encoding the replication-asso-ciated 126 kDa protein in intact plant *** results indicate that transiently expressed shRNA efficientlyinterfered with TMV *** interference observed is sequence-specific,and time-and *** expressed shRNA corresponding to the TMV 126 kDa protein gene did not inhibit cucumbermosaic virus (CMV),an unrelated *** order to interfere with TMV accumulation in tobaccoleaves,it is essential for the shRNA constructs to be infiltrated into the same leaves as TMV *** support the view that RNAi opens the door for novel therapeutic procedures against virus *** propose that a combination of the RNAi technique and Agrobacterium-mediated transient expressioncould be employed as a potent antiviral treatment in plants.

关键词： tobacco mosaic virus 126 kDa protein RNA interference short hairpin RNA

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Electrophoretic Coating of Hydroxyapatite on Pyrolytic Carbon Using Glycol as Dispersion Medium

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Journal of Wuhan university of Technology(Materials Science) 2008年第3期23卷 293-297页

作者：高林 college of Life Science and Technology Huazhong University of Science and Technology college of Chemistry and Environmental Engineering Yangtze UniversityJingzhou 434023China

Hydroxyapatite （HA） coatings on pyrolytic carbon were produced via electrophoretic deposition （EPD） using glycol and ethanol as dispersion medium respectively. The effect of the solubility of HA in the dispersion medium on crack occurrence and adherence of the coating was investigated by means of scanning electronic microscope （SEM） and atomic absorption spectrometer （AAS）. The results show that the solubility of HA in glycol is higher than that in ethanol. The usage of glycol as a dispersion medium can reduce the possibility of crack formation and enhance the adhesive strength between the coating and the carbon substrate. The green coatings can be sintered under vacuum at 1 000 ℃ whether the coatings are obtained using ethanol or glycol as dispersion medium. No HA decomposition was observed up to 1 000 ℃ by vacuum sintering by X-ray diffraction （XRD） analysis.

关键词： hydroxyapatite electrophoretic deposition coatings dispersion medium glycol

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Dual-targeting AAV9P1-mediated neuronal reprogramming in a mouse model of traumatic brain injury

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Neural Regeneration Research 2024年第3期19卷 629-635页

作者： jingzhou Liu Xin Xin Jiejie Sun Yueyue Fan Xun Zhou Wei Gong Meiyan Yang Zhiping Li Yuli Wang Yang Yang Chunsheng Gao State Key Laboratory of Toxicology and Medical Countermeasures Beijing Institute of Pharmacology and ToxicologyBeijingChina Academy of Medical Engineering and Translational Medicine Medical CollegeTianjin UniversityTianjinChina

Traumatic brain injury results in neuronal loss and glial scar *** neurons and eliminating the consequences of glial scar formation are essential for treating traumatic brain *** reprogramming is a promising strategy to convert glial scars to neural ***,previous studies have reported inconsistent *** this study,an AAV9P1 vector incorporating an astrocyte-targeting P1 peptide and glial fibrillary acidic protein promoter was used to achieve dual-targeting of astrocytes and the glial scar while minimizing off-target *** results demonstrate that AAV9P1 provides high selectivity of astrocytes and reactive ***,neuronal reprogramming was induced by downregulating the polypyrimidine tract-binding protein 1 gene via systemic administration of AAV9P1 in a mouse model of traumatic brain *** summary,this approach provides an improved gene delivery vehicle to study neuronal programming and evidence of its applications for traumatic brain injury.

关键词： AAV9P1 astrocytes astrocyte-to-neuron conversion GFAP promoter glial scar induced neurons neuronal reprogramming P1 peptide PTBP1 traumatic brain injury

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Sensitive Determination of DNA by RLS Enhancement of Metal Ions

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Wuhan university Journal of Natural Sciences 2004年第2期9卷 234-238页

作者： Huang Jian-ping 1,2, Chen Fang 1, Ai Xin-ping 1, He Zhi-ke 1 1. college of Chemistry and Molecular Sciences, Wuhan university, Wuhan 430072, Hubei, china 2. Department of Environmental Engineering, Hubei Agricultural college, jingzhou 434025, Hubei, china college of Chemistry and Molecular Sciences Wuhan University Wuhan Hubei China Department of Environmental Engineering Hubei Agricultural College Jingzhou Hubei China

The interactions between metal ions and DNA have been studied by the resonance light scattering (RLS) spectra. In the acidic condition, the RLS signals of metal ions, especially the transition metal ions in group IB and IIB, were increased by DNA. And it is found that the enhancement of RLS signals is linear to the concentration of DNA, so the RLS method for DNA determination was proposed in the presence of Cu2+. On the optimum conditions, the linear range and the detect limit of ctDNA is 4×10?8–4×10?6 g· mL?1 and 1. 13×10?8 g·mL?1, respectively. The proposed method is successfully applied to determine the extracted plasmid DNA ofBacillus subtilis DB104. Key words resonance light scattering - DNA - metal ions CLC number O 657.3 Foundation item: Supported by National Natural Science Foundation of china (20275028) and the National Key Basic Research and Development Program (973 Program 2002CB211800).Biography: Huang Jian-ping(1964-), male, Associate professor, research direction: bioanalysis.

关键词： resonance light scattering DNA metal ions

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Mechanism of production increasing of coalbed gas for the pinnate horizontal well and sensitivity effect of parameters on gas production

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Chinese Science Bulletin 2005年第S1期50卷 163-170页

作者： ZHANG Dongli & WANG Xinhai Sinopec Exploration & Production Research Institute, Beijing 100083, china college of Geoscience of yangtze university, jingzhou 434023, china Sinopec Exploration & Production Research Institute Beijing China college of Geoscience of yangtze university Jingzhou China

The mechanism of production increasing for the pinnate horizontal well is analyzed with the simulation technique. It is pointed out that the whole horizontal well-bore spreading widely and evenly in coal layers makes the stratum pressure drop evenly and fast, which increases the chance of desorption and diffusion and area controlled. This is the basic reason that the pinnate horizontal well can increase production. How such stratum parameters as permeability, Langmuir constants and adsorption time constant affect the gas production for the pinnate horizontal well is also studied with numerical simulations. Either for the vertical well or the pinnate horizontal well, bigger stratum permeability, steeper relative permeability curve, smaller residual gas saturation, higher gas content and stratum pressure are more favorable for gas production increasing. Langmuir constants decide the change of desorption quantity and influence the gas production through changing the extent of steep of isothermal adsorption curves in the pressure dropping area. The adsorption time constant only influences the time that gas production arrives at the maximum value for the vertical well, but it almost has no influence on the gas production for the pinnate horizontal well because the equivalent adsorption time constant is reduced.

关键词： coalbed gas pinnate horizontal well mechanism numerical simulation sensitivity effect.

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Cadmium and Zinc Accumulation in Maize Grain as Affected by Cultivars and Chemical Fixation Amendments

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Pedosphere 2011年第5期21卷 650-656页

作者： GUO Xiao-Fang WEI Ze-Bin WU Qi-Tang QIU Jin-Rong ZHOU Jian-Li Key Laboratory of Ecological agriculture of Ministry of agriculture of china College of Natural Resources and Environ- ment South China Agricultural University Guangzhou 510642 (China) South china Institute of Environmental Sciences Ministry of Environmental Protection Guangzhou 510655 (China) college of agriculture Yangtze University Jingzhou 434025(China)

A field experiment was conducted to investigate the effects of soil amendments(lime,nano-Si foliar solution and used diatomite) on the growth and metal uptake of three maize(Zea mays L.) cultivars grown in a Cd and Zn-contaminated acidic *** addition of lime significantly increased the maize grain yields and decreased the concentrations of Zn and Cd in the grains and shoots of maize when compared with the *** the three maize cultivars,Yunshi-5 accumulated the lowest amounts of Cd and Zn in the *** concentrations of Zn and Cd in the grain of Yunshi-5 conformed to the Chinese feed *** data revealed that a combination of low metal-accumulating maize and chemical fixation could effectively provide a barrier to prevent metals from entering the human food chain.

关键词： grain yield heavy metal low metal-accumulating cultivar Zea mays L.

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Differentially Expression of Tual,a Tubulin-encoding Gene,during Flowering of Tea Plant Camellia sinensis (L.) *** Using cDNA Amplified Fragment Length Polymorphism Technique

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Acta Biochimica et Biophysica Sinica 2006年第9期38卷 653-662页

作者： Wan-Ping FANG~(1,2) Chang-Jun JIANG~1* Mei YU~1 Ai-Hua YE~1 Zhao-Xia WANG~(1,3) 1 Key Laboratory of Tea Biochemistry and Biotechnology,Ministry of agriculture,Anhui Agricultural university,Hefei 230036,china 2 college Of Horticulture,Nanjing agriculture university,Nanjing 210095,china 3 Auhui Institute of Education,Hefei 230036,china Key Laboratory of Tea Biochemistry and Biotechnology Ministry of Agriculture Anhui Agricultural UniversityHefei 230036 China college Of Horticulture Nanjing Agriculture UniversityNanjing 210095 China Auhui Institute of EducationHefei 230036 China

The complementary DNA （cDNA） amplified fragment length polymorphism technique wasused to isolate transcript-derived fragments corresponding to genes involved in the flowering of tea *** sequence analysis of an approximately 300 bp differential fragment amplified by primer combi-nation E11M11revealed 80%-84% similarity to the corresponding part of an α-tubulin gene of other *** complete cDNA sequence of this α-tubulin was cloned by the rapid amplification of cDNA ends technique;its full length is 1537 bp and contains an open reading frame of 450 amino acid residues with two N-glycosylation sites and four protein kinase C phosphorylation *** deduced amino acid sequences didshow significant homology to the α-tubulin from other plants that has been reported to be a pollen-specificprotein and could be correlated with plant cytoplasm-nucleus-interacted male *** named this completecDNA *** nucleotide and amino acid sequence data of Tual have been recorded in the GenBanksequence database with the accession *** Tual gene was cloned into the pET-32aexpression system and expressed in Escherichia coli BL21trxB（DE3）.The molecular weight of expressedprotein was deduced to be approximately 49 *** blot analysis was used to identify the temporalexpression of Tual in tea *** studies of the effect of Tual protein on pollen tube growth indicatedthe Tual solution obviously promoted the growth of tea pollen tube.

关键词： cDNA-AFLP Camellia sinensis flower bud α-tubulin Western blot pollen

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The Growth of Latex Particles during the VAc/BA Copolymerization

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Journal of Wuhan university of Technology(Materials Science) 2006年第3期21卷 86-89页

作者：秦少雄 School of Chemical and Energy Engineering South China University of TechnologyGuangzhou 510640 ChinaCollege of Chemistry and Environment Engineering Yangtze UniversityJingzhou 434100China

The semi-continuous seeded emulsion eopolymerization of vinyl acetate and butyl acrylate was carried out with hydroxyethyl cellulose as a colloid stabilizer. The morphology of the latex particle and the relationship between the reaction time and the average particle diameter and/or the conversion ratio during the polymerization were invstiguted. The experimental results shaw that the morphology of the latex particle possesses the stable sterie construction. In the seeded polymerizution, the average particle diameter of latex decreased while the conversion ratio increased. At the second term of the emulsion copolymerization （the growth stage of particle size）, the latex particle average diameter increased with copolymerization continuously, but the instantaneous conversation ratio was not large, so it was very necessaO to properly prolong the time during the holding temperature stage.

关键词： hydroxyethyl cellulose VAc/ BA latex average particle diameter conversion ratio

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EFFECTS OF TEMPERATURE ON OXYGEN CONSUMPTION AND AMMONIA EXCRETION BY SOLENAIA OLEIVORA

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水生生物学报 2006年第6期30卷 697-701页

作者： XU Qiao-Qing1, LIU Jun2 and HUANG Hua-Wei1 (1. Animal Science college of yangtze university, jingzhou 434025, china 2. Continuous Education college of yangtze university, jingzhou 434025, china) Animal Science college of yangtze university Jingzhou 434025 Continuous Education college of yangtze university Jingzhou 434025

The effects of water temperature on oxygen consumption rate and ammonia excretion rate of Solenaia oleivora were studied in the laboratory. The results showed that, under controlled conditions and ambient temperatures 15—30℃, the relationship between oxygen consumption rate (O) [mg/h] and dry weight of soft tissue (W) [g] can be represented by an allometric equation O=aW b, while the relationship between ammonia excretion rate (N) [μg/h] and dry weight of soft tissue (W) [g] follows also an allometric equation N=cW b. It is indicated that both metabolic rates are correlated positively with water temperature. High temperature can reduce the level of protein metabolism. The linear regression among oxygen consumption rate (O), temperature (T) and dry weight of soft tissue (W) can be described by the equation O=-0.6513+0.0532T+0.1073W, and for ammonia this relation is N=32.1626-1.0566T+1.3222W, the multiple relation coefficient was 0.9642 and 0.8921, respectively.

关键词： Solenaia oleivora Temperature Oxygen consumption rate Ammonia excretion rate

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Coupling analysis of unsteady seepage and stress fields in discrete fractures network of rock mass in dam foundation

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Science china(Technological Sciences) 2011年第S1期54卷 133-139页

作者： CHAI JunRui 1,2 & XU WeiSheng 2 1 college of Hydraulic and Environmental Engineering,china Three Gorges university,Yichang 443002,china 2 college of Hydropower Engineering,Xi’an university of Technology,Xi’an 710048,china college of Hydraulic and Environmental Engineering China Three Gorges University Yichang China college of Hydropower Engineering Xi’an University of Technology Xi’an China

The drag force of water flow through single fracture and the coupling characteristics of seepage and stress in single fracture surface are analyzed,and a three dimensional model of coupled unsteady seepage and stress fields is *** model is used to the analysis of foundation rock mass of a high *** the coupling effects are considered,the changes of boundary heads have less influence on the inner head of rock mass,and the strong permeability of main fractures *** the coupling effects are not considered,the fractures distribution affects the inner head more *** the upstream water head declines,the inner head of dam foundation slightly declines and the hydraulic gradient distribution becomes smoother.A bigger upstream water level declining velocity has a stronger lag effect,meanwhile the values of stress components change more *** the upstream water level declining velocity directly affects the stability of rock mass in dam foundation and we should take into account the above factors to make sure the safety of the dam during reservoir level fluctuation period.

关键词： hydraulic engineering unsteady seepage stress coupling fractures network rock mass

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