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Structure of African swine fever virus p15 reveals its dual role for membrane-association and DNA binding

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Protein & Cell 2020年第8期11卷 606-612页

作者： Dan Fu Dongming Zhao Wei Zhang Guangshun Zhang Mingyu Li Zheng Zhang Yuhui Wang Dongdong Sun Peng Jiao cheng chen Yu Guo zihe Rao State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy Nankai UniversityTianjin 300350People's Republic of China State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150001People's Republic of China School of Life Sciences Tianjin UniversityTianjin 300071People's Republic of China

Dear Editor, African swine fever (ASF) is a highly contagious disease of domestic pigs caused by African swine fever virus (ASFV),with mortality rates approaching 90%-100%.The first ASF case was described in 1921 in *** the past decades, ASF has spread from Sub-Saharan Africa to Europe and Asia, and pose a huge threat to global food *** the first ASF case reported in August 2018, more than 10 million pigs had been culled in China at an enormous economic *** risk of ASFV mutating to infect humans is extremely ***, ASF not only threatens food security but also severely strikes the world economy in many fields, such as the global supply of the blood thinner heparin,most of which is produced from ***, ASF is a strictly monitored infectious animal disease by OIE (World Organization for Animal Health), unfortunately, there is no approved drug or vaccine against this lethal viral disease.

关键词： swine fever African

Extracellular vesicles derived from neural EGFL-Like 1-modified mesenchymal stem cells improve acellular bone regeneration via the miR-25-5p-SMAD2 signaling axis

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Bioactive Materials 2022年第11期7卷 457-470页

作者： Yanhua Lan Huizhi Xie Qianrui Jin Xiaomin Zhao Yang Shi Yanyan Zhou zihe Hu Yi Ye Xiaoyuan Huang Yingjia Sun Zhuo chen Zhijian Xie Stomatology Hospital School of StomatologyZhejiang University School of MedicineClinical Research Center for Oral Diseases of Zhejiang ProvinceKey Laboratory of Oral Biomedical Research of Zhejiang ProvinceCancer Center of Zhejiang UniversityHangzhou310006China Department of Orthopaedics and Traumatology Li Ka Shing Faculty of MedicineThe University of Hong KongPokfulamHong Kong999077China

Stem cell based transplants effectively regenerate tissues;however, limitations such as immune rejection and teratoma formation prevent their application. Extracellular vesicles (EVs)-mediated acellular tissue regeneration is a promising alternative to stem cell based transplants. Although neural EGFL-like 1 (Nell1) is known to contribute to the osteogenic differentiation of bone marrow stem cells (BMSCs), it remains unknown whether EVs are involved in this process. Here, we present that EVs derived from Nell1-modified BMSCs (Nell1/EVs) have a stronger ability to promote BMSC osteogenesis owing to miR-25–5p downregulation. MiR-25–5p inhibits osteogenesis by targeting Smad2 and suppressing the SMAD and extracellular signal-related kinase 1 and 2 (ERK1/2) pathway activation. In addition, we demonstrate that the 3D-Nell1/EV-hydrogel system is beneficial for bone regeneration in vivo, probably stemming from a slow, continuous release and high concentration of EVs in the bone defect area. Thus, our results have shown the potential of Nell1/EVs as a novel acellular bone regeneration strategy. Mechanistically, the identification of miR-25-5p-SMAD2 signaling axis expands the knowledge of Nell1/EVs induced osteogenesis.

关键词： Extracellular vesicles Mesenchymal stem cells Bone regeneration Cell-free scaffolds miRNAs

Relationships between Features of EmergingAdulthood, Situated Decisions toward PhysicalActivity, and Physical Activity among CollegeStudents: The Moderating Role of ExerciseIntensity Tolerance

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International Journal of Mental Health Promotion 2023年第11期25卷 1209-1217页

作者： Jinghua chen zihe Wang Fabian Herold Alyx Taylor Jin Kuang Ting Wang Arthur F.Kramer Liye Zou Body-Brain-Mind Laboratory School of PsychologyShenzhen UniversityShenzhen518060China Beijing Chaoyang Hospital Capital Medical UniversityBeijing100020China Research Group Degenerative and Chronic Diseases MovementFaculty of Health Sciences BrandenburgUniversity of PotsdamPotsdam14476Germany School of Rehabilitation Sport and PsychologyAECC University CollegeBournemouthBH52DFUK Center for Cognitive&Brain Health Northeastern UniversityBoston02115-5005USA Beckman Institute University of IllinoisIllinoisUSA

A significant portion of emerging adults do not achieve recommended levels of physical activity (PA). Previous studies observedassociations between features of emerging adulthood and PA levels, while the potential psychological mechanisms that mightexplain this phenomenon are not fully understood. In this context, there is some evidence that situated decisions towardphysical activity (SDPA) and exercise-intensity tolerance might influence PA level. To provide empirical support for thisassumption, the current study investigated whether (i) features of emerging adulthood are linked to SDPA, which, in turn,might affect PA engagement;(ii) exercise-intensity tolerance moderate the relationship between SDPA and PA level;and (iii)SDPA is a mediator of the relationship between features of emerging adulthood and PA levels under the prerequisite thatexercise-intensity tolerance moderates the link between SDPA and PA engagement. In this study a group of 1,706 Chinesecollege students was recruited and asked to complete a set of questionnaires assessing their SDPA, PA levels, exercise-intensitytolerance, and features associated with emerging adulthood, namely Self-exploration, Instability, and Possibility. Our resultsindicated that SDPA positively predicted PA levels and this relationship became stronger when exercise-intensity tolerance wasused as a moderator. Furthermore, it was observed that individuals with a higher level of Instability and a lower level ofPossibility during emerging adulthood exhibited a lower level of SDPA. Taken together, the results of our study providefurther insights on a potential psychological mechanism linking features of emerging adulthood and physical activity.

关键词： Emerging adulthood situated decisions physical activity exercise-intensity tolerance moderated model

Preparation of the selenome-thionine derivative of tab-toxin resistance protein

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Chinese Science Bulletin 2001年第15期46卷 1304-1307页

作者： HE Hongzhen SHAO Yu chen Yuhang LIU Bingbing CAO Zhenbo JIANG Fan LIU Yiwei TANG Hong LIU Jinyuan ZHAO Nanming RAO zihe Laboratory of Structural Biology&Protein Science Laboratory of MOE School of Life Science and EngineeringTsinghua UniversityBeijing 100084China

In order to obtain phase information for the X-ray diffraction of tabtoxin resistance protein (TTR) crystal using the MAD phasing method, a selenomethionine (Se-Met) derivative of TTR was overexpressed in E. coli strain M15, with pQE-30 plasmid, through IPTG induction in M9 medium containing Se-Met. The product was purified to an estimated homogeneity of greater than 95% according to SDS-PAGE, by a Ni-NTA metal affinity followed by a Mono Q anion exchange column chromatography. The successful substitution of Se-Met for methionine (Met) was confirmed by MALDI-TOF and ESI-Quadrupole Mass Spectrometry analysis. The derivative crystal was obtained using similar conditions as those for the native.

关键词： Se-Met TTR derivative expression purification mass spectrometry.

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欧李营养价值及开发利用研究进展

中国果树 2024年第2期 1-5,25页

作者：吴春燕韩潇怡吴俊英王薇谢琛肖宝莹周子贺董新静吉林农业大学园艺学院长春130118 内蒙古赤峰市巴林左旗农牧技术推广中心

欧李是我国特有的野生灌木果树,果、仁、花、叶、根等均可利用,具有显著的经济、药用、生态和观赏价值。综述了欧李的营养价值和当前开发利用情况,探讨了开发利用过程中存在的问题并进行了展望,以期为今后欧李的综合开发利用提供参考和... 详细信息

欧李是我国特有的野生灌木果树,果、仁、花、叶、根等均可利用,具有显著的经济、药用、生态和观赏价值。综述了欧李的营养价值和当前开发利用情况,探讨了开发利用过程中存在的问题并进行了展望,以期为今后欧李的综合开发利用提供参考和理论基础。

关键词：欧李营养价值开发利用

Crystal structure of the African swine fever virus structural protein p35 reveals its role for core shell assembly

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Protein & Cell 2020年第8期11卷 600-605页

作者： Guobang Li Dan Fu Guangshun Zhang Dongming Zhao Mingyu Li Xue Geng Dongdong Sun Yuhui Wang cheng chen Peng Jiao Lin Cao Yu Guo zihe Rao State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy Nankai UniversityTianjin 300350China State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150001China School of Life Sciences Tianjin UniversityTianjin 300071China

Dear Editor,The African swine fever(ASF)is a highly contagious hemorrhagic and lethal disease in domestic *** ASF outbreaks of in many Asia countries,including China,Vietnam,Mongolia,and South Korea,have posed a huge threat to the pig *** the attempt to stop ASF from spreading further in China's Mainland,more than 10 million pigs have been culled since August *** causative agent for ASF is a DNA virus,African swine fever virus(ASFV).Although this deadly disease has been reported in Kenya nearly one hundred years ago,there has been no vaccine for protection from ASFV infection or effective treatments to cure ASF until now.

关键词： swine fever African

LiMn2O4 with hierarchical porous morphology obtain an excellent electrochemical performance

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LiMn2O4 with hierarchical porous morphology obtain an excell...

酱香轮次不同层次窖内发酵酒醅理化指标变化规律的分析研究

河南省化学会2016年学术年会

作者： zihe Li Weihua chen Liwei Mi Xiangming Feng College of chemistry and Molecular Engineering Zhengzhou University Center for Advanced Materials Research Zhongyuan University of Technology

As one of important positive materials for Li-ion batteries, LiMnO have been commercialized in recent years, and LiMnO-based batteries have been successful applied in Electric Vehicles and Hybrid Electric Vehicles. To improve the LiMnO electrochemical performance, some attention is paying to the control of morphologies, just like cubic and sphere. However, various morphologies haven’t been compared in the electrochemical field, and draw a conclusion about the cause of formulation. So, we could manipulate the key factor to template the morphology. Herein, we attempt to control p H value and surfactant for synthesizing the cubic, sphere, onion-like Mn2O3 precursor and probe their generation mechanism. Based on the prepared MnO, we fabricated the corresponding morphology spinel LiMnO by calcining LiOH and precursor MnO described above. The onion shaped LiMnO inherits an excellent morphology from a hierarchical onion sphere MnO tend to present an excellent electrochemical performance, it possesses the best rate capacity with 139 m Ah g at 0.05 C, it retention 88.6 % as the current rate rising from 0.05 C to 2C.

关键词： LiMn2O4 pH value surfactant morphology

来源： cnki会议评论

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酿酒科技 2024年第3期 123-126页

作者：张翔张福艳周平舒楠康忠媛魏立男李子和陈家好湖南武陵酒有限公司湖南常德415000 河北省固态发酵产业技术研究院河北衡水053000 河北衡水老白干酒业股份有限公司河北衡水053000

以湖南某公司酿酒车间2个班组全周期窖池为研究对象,跟踪窖内不同位置发酵酒醅理化指标的变化情况。结果表明,酒醅的水分、酸度随着轮次推进逐渐增加,淀粉含量逐渐减少;同一轮次中,上层酒醅的水分总体高于中层和下层,不同位置酒醅的酸... 详细信息

以湖南某公司酿酒车间2个班组全周期窖池为研究对象,跟踪窖内不同位置发酵酒醅理化指标的变化情况。结果表明,酒醅的水分、酸度随着轮次推进逐渐增加,淀粉含量逐渐减少;同一轮次中,上层酒醅的水分总体高于中层和下层,不同位置酒醅的酸度表现为下层>中层>上层,淀粉含量总体差异不大。

关键词：酱香型白酒理化指标变化规律

Structural basis of Zika virus helicase in recognizing its substrates

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Protein & Cell 2016年第8期7卷 562-570页

作者： Hongliang Tian Xiaoyun Ji Xiaoyun Yang Zhongxin Zhang zuoku Lu Kailin Yang cheng chen Qi Zhao Heng Chi Zhongyu Mu Wei Xie Zefang Wang Huiqiang Lou Haitao Yang zihe Rao School of Life Sciences Tianjin University Tianjin 300072 China Tianjin Intemational Joint Academy of Biotechnology and Medicine Tianjin 300457 China The State Key Laboratory of Pharmaceutical Biotechnology School of Life Sciences Nanjing University Nanjing 210023 China State Key Laboratory of Agro-Biotechnology College of Biological Sciences China Agricultural University Beijing 100193 China College of Life Sciences Nankai University Tianjin 300071 China Cleveland Clinic Lemer College of Medicine of Case Western Reserve University Cleveland OH 44195 USA Department of Molecular Biophysics and Biochemistry Yale University New Haven CT 06520 USA

The recent explosive outbreak of Zika virus （ZlKV） infection has been reported in South and Central America and the Caribbean. Neonatal microcephaly associated with ZIKV infection has already caused a public health emergency of international concern. No specific vaccines or drugs are currently available to treat ZIKV infection. The ZIKV helicase, which plays a pivotal role in viral RNA replication, is an attractive tar- get for therapy. We determined the crystal structures of ZIKV helicase-ATP-Mnz＋ and ZlKV helicase-RNA. This is the first structure of any flavivirus helicase bound to ATP. Comparisons with related flavivirus helicases have shown that although the critical P-loop in the active site has variable conformations among different species, it adopts an identical mode to recognize ATPIMn2＋. The structure of ZlKV helicase-RNA has revealed that upon RNA binding, rotations of the motor domains can cause significant conformational changes. Strikingly, although ZlKV and dengue virus （DENV） apo-helicases share conserved residues for RNA binding, their different manners of motor domain rotations result in distinct individual modes for RNA recognition. It suggests that flavivirus helicases could have evolved a conserved engine to convert chemical energy from nucleoside triphosphate to mechanical energy for RNA unwinding, but different motor domain rotations result in variable RNA recognition modes to adapt to individual viral replication.

关键词： Zika virus helicase ATP crystal structure,flavivirus