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Origin and phylogenetic analysis of Tibetan Mastiff based on the mitochondrial DNA sequence

Journal of Genetics and Genomics 2008年第6期35卷 335-340页

作者： Qifa Li Zhenshan Liu Yinxia Li Xingbo Zhao liyan Dong Zengxiang Pan Yuanrong Sun Ning Li Yinxue Xu zhuang Xie Laboratory of Animal Reproductive Genetics and Molecular Evolution College of Animal Science and Technology Nanjing Agricultural University Nanjing 210095 China State Key Laboratory for Agribiotechnology China Agricultural University Beijing 100094 China

At present, the Tibetan Mastiff is the oldest and most ferocious dog in the world. However, the origin of the Tibetan Mastiff and its phylogenetic relationship with other large breed dogs such as Saint Bernard are unclear. In this study, the primers were designed accord- ing to the mitochondrial genome sequence of the domestic dog, and the 2,525 bp mitochondrial sequence, containing the whole sequence of Cytochrome b, tRNA-Thr, tRNA-Pro, and control region of the Tibetan Mastiff, was obtained. Using grey wolves and coyotes as out- groups, the Tibetan Mastiff and 12 breeds of domestic dogs were analyzed in phylogenesis. Tibetan Mastiff, domestic dog breeds, and grey wolves were clustered into a group and coyotes were clustered in a group separately. This indicated that the Tibetan Mastiff and the other domestic dogs originated from the grey wolf, and the Tibetan Mastiff belonged to Carnivora, Canidae, Canis, Canis lupus, Canis lupus familiaris on the animal taxonomy. In domestic dogs, the middle and small breed dogs were clustered at first; German Sheepdog, Swedish Elkhound, and Black Russian Terrier were clustered into one group, and the Tibetan Mastiff, Old English Sheepdog, Leonberger, and Saint Bernard were clustered in another group. This confirmed the viewpoint that many of the famous large breed dogs worldwide such as Saint Bernard possibly had the blood lineage of the Tibetan Mastiff, based on the molecular data. According to the substitution rate, we concluded that the approximate divergence time between Tibetan Mastiff and grey wolf was 58,000 years before the present （YBP）, and the approximate divergence time between other domestic dogs and grey wolf was 42,000 YBP, demonstrating that the time of origin of the Tibetan Mastiff was earlier than that of the other domestic dogs.

关键词： Tibetan Mastiff domestic dog mitochondrial DNA origin taxonomic status phylogenetic relationship

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基于ESP-32的环网柜智能锁具系统

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大众科学 2024年第19期45卷 7-9页

作者：庄丽燕杨伟财泉州亿兴电力工程建设有限公司丰泽配电分公司福建泉州362008

在电力网络中,环网柜承担着连接环网线路、增强电力供应的稳定性与可靠性的关键角色,对保障电力系统的安全与稳定至关重要.环网柜的门锁安全是保障环网柜安全的极其关键的一环.目前环网柜普遍使用的是传统的机械锁,这种锁具只要持有相... 详细信息

在电力网络中,环网柜承担着连接环网线路、增强电力供应的稳定性与可靠性的关键角色,对保障电力系统的安全与稳定至关重要.环网柜的门锁安全是保障环网柜安全的极其关键的一环.目前环网柜普遍使用的是传统的机械锁,这种锁具只要持有相应的钥匙,任何人都能够轻易地打开,这无疑对电力系统的安全性构成了潜在的威胁.为解决此问题,介绍了一种基于ESP-32的环网柜智能锁具系统,该锁具可通过蓝牙通信技术,实现锁具的无线控制,消除了物理钥匙丢失或被复制的风险;同时,智能锁具系统可以设置多级访问权限,只有经过授权的用户才能解锁,从而有效防止了未授权访问;另外,系统还能够记录每次开锁的时间和用户信息,为电力系统的安全管理提供了可靠的数据支持,通过智能化管理,提高了智能锁安全性.

关键词：环网柜智能锁蓝牙 ESP-32

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Application of Pyroligneous Acid in the Prevention and Control of Brown Blotch Disease in Pleurotus ostreatus

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Agricultural Science & Technology 2016年第9期17卷 2103-2105,2170页

作者： Yanyan XU liyan ZHENG zhuang LI Jie LI College of Biology and Food Science Hebei Normal University for Nationalities College of Life Science Hebei Normal University

In the present study, agar diffusion test and field trials were carried out using a mushroom （Pleurotus ostreatus） culti- var Shouyanping as the experimental material to determine the antibacterial activity of refined pyroligneous acid prepared from almond shell against Pseudomonas tolaasfi and its effects on mycelial growth of mushroom. The results revealed that 1.25%, 2.50% and 5.00% of refined pyroligneous acid obviously inhibited the occurrence of brown blotch disease in mushroom, but also greatly promoted mushroom mycelial growth. Therefore, 1.25% and 2.50% of refined pyroligneous acid can be used in fields to prevent brown blotch disease, and 5.00% refined pyroligneous acid can be used to control the incidence of brown blotch disease when it widely occurs in fields.

关键词： Almond shell pyroligneous acid Pleurotus ostreatus Brown blotch disease Pseudomonas tolaasii

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人力资本流动、空间溢出与经济增长:新经济地理学的视角

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东南学术 2022年第3期 148-158页

作者：郭丽燕庄惠明福建商学院工商管理学院福建商学院国际经贸学院

当前,随着人才政策的不断推行、户籍制度的不断改革、高铁等交通网络的不断完善,中国的人力资本要素发生了大规模的跨区域空间流动。人力资本空间流动对挖掘人力资本红利、促进经济增长具有重要意义。我国地级市单元间的经济增长及人力... 详细信息

当前,随着人才政策的不断推行、户籍制度的不断改革、高铁等交通网络的不断完善,中国的人力资本要素发生了大规模的跨区域空间流动。人力资本空间流动对挖掘人力资本红利、促进经济增长具有重要意义。我国地级市单元间的经济增长及人力资本流动存在显著的空间相关性,人力资本流动的直接经济增长效应和空间溢出增长效应较为显著,且人力资本流动所伴随的知识或技术的空间溢出效应对区域经济增长的贡献高于其直接效应;西部地区人力资本流动的空间溢出效应对区域经济增长的贡献明显大于东部地区和中部地区。在开发利用有限人力资本要素的同时,应大力推进人力资本在区域间的自由、充分、高效流动,充分发挥人力资本流动的空间溢出效应,以此推动我国经济增长。

关键词：人力资本流动经济增长新经济地理学空间溢出

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基于混合消能减震技术的组合结构独柱高架站台抗震性能研究

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建筑结构学报 2019年第5期40卷 50-62页

作者：许立言聂鑫庄亮东陶慕轩清华大学土木工程系北京100084 清华大学土木工程安全与耐久教育部重点实验室北京100084 中国铁道科学研究院高速铁路轨道技术国家重点实验室北京100081 清华大学北京市钢与混凝土组合结构工程技术研究中心北京100084

结合高性能组合结构形式以及混合消能减震体系的双重技术优势,研发了适用于独柱长悬臂式高架站台结构的抗震体系。采用有限元分析软件MSC. MARC分别对该体系进行了强震作用下的静力弹塑性分析和动力弹塑性时程分析。分析结果表明基于MSC... 详细信息

结合高性能组合结构形式以及混合消能减震体系的双重技术优势,研发了适用于独柱长悬臂式高架站台结构的抗震体系。采用有限元分析软件MSC. MARC分别对该体系进行了强震作用下的静力弹塑性分析和动力弹塑性时程分析。分析结果表明基于MSC. MARC二次开发的纤维模型和采用宏观本构的一维非线性弹簧模型能高效准确地模拟站台结构的非线性动力特征以及损伤破坏规律。混合消能减震技术可显著提高结构的刚度和承载力,在降低主体构件耗能的同时增强结构的整体耗能能力。在体系层面,混合消能减震技术可明显改善结构刚度分布不均匀带来的不利影响,有效地降低结构的整体变形和层间位移角;在构件层面,消能减震体系能减少或避免主体构件的出铰,有效地降低主要承重构件的截面应力水平,降低悬臂梁所承受的扭矩。值得注意的是,在混合消能减震体系中,由于结构的各个薄弱环节均得到了加强,未受到消能减震构件保护的其他结构构件的地震响应可能会被放大。

关键词：组合结构独柱高架站台混合消能减震技术有限元分析抗震性能

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Characterization of humoral immune responses against SARS-CoV-2 accessory proteins in infected patients and mouse model

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Virologica Sinica 2024年第3期39卷 414-421页

作者： Yuming Li Yanhong Tang Xiaoqian Wang Airu Zhu Dongdong Liu Yiyun He Hu Guo Jie Zheng Xinzhuo Liu Fengyu Chi Yanqun Wang Zhen zhuang Zhaoyong Zhang Donglan Liu Zhao Chen Fang Li Wei Ran Kuai Yu Dong Wang liyan Wen Jianfen Zhuo Yanjun Zhang Yin Xi Jingxian Zhao Jincun Zhao Jing Sun School of Public Health Shandong First Medical University&Shandong Academy of Medical SciencesJi'nan250117China Key Laboratory of Emerging Infectious Diseases in Universities of Shandong Shandong First Medical University&Shandong Academy of Medical SciencesJi'nan250117China State Key Laboratory of Respiratory Disease National Clinical Research Center for Respiratory DiseaseNational Center for Respiratory MedicineGuangzhou Institute of Respiratory HealthThe First Affiliated Hospital of Guangzhou Medical UniversityGuangzhouGuangdong510182China Hunan Provincial People's Hospital The First Affiliated Hospital of Hunan Normal UniversityHunan Normal UniversityChangsha410005China Guangzhou National Laboratory GuangzhouGuangdong510005China Shanghai Institute for Advanced Immunochemical Studies School of Life Science and TechnologyShanghai Tech UniversityShanghai201210China Institute for Hepatology National Clinical Research Center for Infectious DiseaseShenzhen Third People's HospitalThe Second Affiliated HospitalSchool of MedicineSouthern University of Science and TechnologyShenzhen518005China

Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the causative agent of COVID-19,encodes several accessory proteins that have been shown to play crucial roles in regulating the innate immune ***,their expressions in infected cells and immunogenicity in infected humans and mice are still not fully *** study utilized various techniques such as luciferase immunoprecipitation system(LIPS),immunofluorescence assay(IFA),and western blot(WB)to detect accessory protein-specific antibodies in sera of COVID-19 *** antibodies to proteins 3a,3b,7b,8 and 9c can be detected by LIPS,but only protein 3a antibody was detected by IFA or *** against proteins 3a and 7b were only detected in ICU patients,which may serve as a marker for predicting disease ***,we investigated the expression of accessory proteins in SARS-CoV-2-infected cells and identified the expressions of proteins 3a,6,7a,8,and *** also analyzed their ability to induce antibodies in immunized mice and found that only proteins 3a,6,7a,8,9b and 9c were able to induce measurable antibody productions,but these antibodies lacked neutralizing activities and did not protect mice from SARS-CoV-2 *** findings validate the expression of SARS-CoV-2 accessory proteins and elucidate their humoral immune response,providing a basis for protein detection assays and their role in pathogenesis.

关键词： SARS-CoV-2 Accessory protein Humoral immune responses COVID-19 Mouse model

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人力资本流动、高新技术产业集聚与经济增长

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南开经济研究 2020年第6期 163-180页

作者：郭丽燕黄建忠庄惠明华侨大学经济与金融学院 362021 福建商学院工商管理学院 350012 上海对外经贸大学国际经贸学院 201620 福建商学院国际经贸学院 350012

本文旨在考察人力资本流动、高新技术产业集聚对经济增长的影响及其内在机制。在理论分析的基础上,本文以我国省际单元为研究对象,考虑经济体之间的相互关联和经济增长惯性,运用空间动态面板模型进行了实证检验。研究结果显示:我国地区... 详细信息

本文旨在考察人力资本流动、高新技术产业集聚对经济增长的影响及其内在机制。在理论分析的基础上,本文以我国省际单元为研究对象,考虑经济体之间的相互关联和经济增长惯性,运用空间动态面板模型进行了实证检验。研究结果显示:我国地区经济增长存在明显的空间依赖和空间相关性;人力资本流动、高新技术产业集聚均对地区经济增长有显著的正向促进作用,且二者在推动经济增长的过程中相互作用,进一步促进地区经济增长;考虑不同的空间权重、模型设定偏误和互为因果关系的内生性后,模型估计结果依然稳健。

关键词：人力资本流动高新技术产业集聚经济增长空间动态面板

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奈达铂化疗同步三维适形放疗治疗中晚期食管癌的临床观察

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胃肠病学 2010年第8期15卷 486-488页

作者：汪丽燕李滨庄丽维哈尔滨医科大学附属第四医院消化内科

食管癌常规放疗疗效欠佳，局部复发是造成放疗失败的主要原因。目的：探讨奈达铂（NDP）化疗同步三维适形放疗（3-DCRT）治疗中晚期食管癌的近期疗效、近期毒副反应和远期疗效。方法：75例经病理证实的中晚期食管癌患者随机分成放疗组... 详细信息

食管癌常规放疗疗效欠佳，局部复发是造成放疗失败的主要原因。目的：探讨奈达铂（NDP）化疗同步三维适形放疗（3-DCRT）治疗中晚期食管癌的近期疗效、近期毒副反应和远期疗效。方法：75例经病理证实的中晚期食管癌患者随机分成放疗组（37例，6 MV-X线或15 MV-X线常规放疗，2.0 Gy/次，5次/周，6~7周）和同期放化组（38例，放射治疗始即同步行NDP化疗，30~40 mg，静脉滴入，1次/周，共6次）。治疗结束后3个月评价近期疗效和近期毒副反应，随访结束后评价远期疗效。结果：同期放化组的总有效率高于放疗组，差异具有统计学意义（78.9%对51.4%，P＜0.05）；放疗组和同期放化组的2级及其以上、3级及其以上放射性食管炎发生率差异不具有统计学意义（40.5%对50.0%、10.8%对18.4%，P＞0.05）。同期放化组的1、3年局部控制率（63.6％对73.0％，43.3%对49.2%，P＜0.05）和1、3年生存率（69.8％对49.8％、41.3％对30.2%，P＜0.05）均显著高于放疗组。结论：DNP化疗同步放疗治疗中晚期食管癌的近、远期疗效优于放疗组，且患者能耐受近期毒副反应，因此具有一定临床应用前景。

关键词：食管肿瘤奈达铂放射治疗治疗结果

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Multi-platform omics analysis reveals molecular signature for COVID-19 pathogenesis,prognosis and drug target discovery

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Signal Transduction and Targeted Therapy 2021年第5期6卷 1535-1545页

作者： Yuming Li Guixue Hou Haibo Zhou Yanqun Wang Hein Min Tun Airu Zhu Jingxian Zhao Fei Xiao Shanwen Lin Dongdong Liu Dunrong Zhou Lang Mai Lu Zhang Zhaoyong Zhang Lijun Kuang Jiao Guan Qiushi Chen liyan Wen Yanjun Zhang Jianfen Zhuo Fang Li Zhen zhuang Zhao Chen Ling Luo Donglan Liu Chunke Chen Mian Gan Nanshan Zhong Jincun Zhao Yan Ren Yonghao Xu State Key Laboratory of Respiratory Disease National Clinical Research Center for Respiratory DiseaseGuangzhou Institute of Respiratory Healththe First Affiliated Hospital of Guangzhou Medical UniversityGuangzhouChina BGI-Shenzhen ShenzhenChina The Sixth Affiliated Hospital of Guangzhou Medical University Qingyuan People's HospitalQingyuanGuangdongChina HKU-Pasteur Research Pole School of Public HealthLi Ka Shing Faculty of MedicineThe University of Hong KongHong Kong SARChina Department of Infectious Diseases Guangdong Provincial Key Laboratory of Biomedical ImagingGuangdong Provincial Engineering Research Center of Molecular ImagingThe Fifth Affiliated HospitalSun Yat-sen UniversityZhuhaiGuangdongChina Yangjiang People's Hospital YangjiangGuangdongChina Yangjiang Center for Disease Control and Prevention YangjiangGuangdongChina Guangzhou Eighth People's Hospital of Guangzhou Medical University GuangzhouGuangdongChina Guangzhou Customs District Technology Center GuangzhouChina

Disease progression prediction and therapeutic drug target discovery for Coronavirus disease 2019(COVID-19)are particularly important,as there is still no effective strategy for severe COVID-19 patient ***,we performed multi-platform omics analysis of serial plasma and urine samples collected from patients during the course of *** analyses of these omics data revealed several potential therapeutic targets,such as ANXA1 and *** changes in plasma indicated dysregulation of macrophage and suppression of T cell functions in severe patients compared to those in non-severe ***,we chose 25 important molecular signatures as potential biomarkers for the prediction of disease *** prediction power was validated using corresponding urine samples and plasma samples from new COVID-19 patient cohort,with AUC reached to 0.904 and 0.988,*** conclusion,our omics data proposed not only potential therapeutic targets,but also biomarkers for understanding the pathogenesis of severe COVID-19.

关键词： pathogenesis urine drug

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Analysis of IGF2 mRNA expression and its methylation status between cattle yaks and their parents

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Progress in Natural Science:Materials International 2009年第9期19卷 1063-1069页

作者： Zhenshan Liu a,b,1, Qifa Li a,1, Qingbo Zhang a, Xuguang Qu a, liyan Dong a, Chamba Yang-zom c, zhuang Xie a,*, Honglin Liu a a Laboratory of Animal Reproductive Genetics and Molecular Evolution, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China b Institute of Animal Husbandry and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, China c College of Agriculture and Animal Husbandry, Tibet University, Linzhi 860000, China Laboratory of Animal Reproductive Genetics and Molecular Evolution College of Animal Science and Technology Nanjing Agricultural University Nanjing 210095 China Institute of Animal Husbandry and Veterinary Medicine Shandong Academy of Agricultural Sciences Jinan 250100 China College of Agriculture and Animal Husbandry Tibet University Linzhi 860000 China

Cattle yaks are an F1 hybrid between cattle and yaks and exhibit significant hybrid vigor. However, cattle yaks are sterile males. To study the epigenetic aspects of this reproductive isolation, IGF2 mRNA expression in cattle yaks and their parents and the patterns of the IGF2 differentially methylated regions (DMR) were examined in blood and testes. The results showed that IGF2 expression in cattle yaks’ testes was lower than in their parents (P 0.05). Our study showed that IGF2 plays an important role in bovine spermato-genesis and might be involved in cattle-yak male sterility. The methylation level of the IGF2 DMR was irrelevant to the lower expression of IGF2 in cattle yaks; other factors perhaps play roles in its expression.

关键词： Cattle yak Male sterility IGF2 gene mRNA expression DNA methylation

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