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Biomedical and Environmental Sciences 2019年第10期32卷 755-768页

作者： ZHANG Jia Yan XIAO Xiang DONG Ying zhou xing hua School of Food and Biological Engineering Jiangsu University

Objective To further explore associated effects of Lactobacillus plantarum dy-1(LFBE) on obesity and lipid metabolism at the gene expression level, the expression of micro RNAs(mi RNAs) was investigated in the liver of high-fat diet(HFD) induced obese *** Three groups of animal models were established. Changes in mi RNA expression in the liver of each group were analyzed by microarray and RT-q PCR, complemented by bioinformatics. Palmitateinduced hepatocellular carcinoma(Hep G2) cells were used as a model to validate the *** LFBE treatment groups and HFD groups were observed to be distinctly different with respect to rates of increase in body weight and body fat percentage and triglyceride(TG) and total cholesterol(TC) levels in serum and liver. In addition, the LFBE group showed upregulation of ten mi RNAs and downregulation of five mi RNAs in the liver. Downregulation of mi R-34 a and mi R-212 was observed in the livers of the LFBE group. Gene ontology and kyoto encyelopedia of geues and genomes(KEGG)pathway analysis showed that possible target genes of the deregulated mi RNAs were significantly enriched in the adrenergic and HIF-1 signaling *** These results demonstrate that LFBE might regulate the expression of mi RNAs in order to inhibit obesity and fatty liver.

关键词： Fermented barley extract Lactobacillus plantarum dy-1 Anti-obesity Lipid metabolism miRNAs Microarray

Fermented Barley Extracts with Lactobacillus plantarum dy-1 Rich in Vanillic Acid Modulate Glucose Consumption in Human HepG2 Cells

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Biomedical and Environmental Sciences 2018年第9期31卷 667-676页

作者： ZHANG Jia Yan XIAO Xiang DONG Ying zhou xing hua School of Food and Biological Engineering Jiangsu University

Objective To investigate the effect of fermented barley extracts with Lactobacillus plantarum dy-1（LFBE） for modulating glucose consumption in HepG2 cells via miR-212 regulation. Methods Hepatocellular carcinoma（HepG2） cells were treated with palmitate. After 12 h, palmitate-induced HepG2 cells were treated with LFBE and its main components. Changes in glucose consumption, proinflammatory cytokine secretion, and miRNA-212 expression in HepG2 cells was observed. Results Treatment with LFBE rich in vanillic acid（VA） increased glucose consumption and reduced proinflammatory cytokine secretion in HepG2 cells. LFBE and VA normalized the upregulation of miR-212, which led to the upregulation of dual-specificity phosphatase-9（DUSP9）, a direct target of miR-212, at both protein and mR NA levels. Downregulation of miR-212 markedly increased glucose consumption and reduced proinflammatory cytokine secretion by enhancing DUSP9 expression. Conclusion The results showed the benefit of LFBE and miR-212 downregulation in modulating glucose consumption and reducing proinflammatory cytokine secretion by targeting DUSP9. VA in LFBE was a strong regulator of palmitate-induced abnormal glucose consumption in HepG2 cells and can be a primary mediator.

关键词： Fermented barley extract Vanillic acid Glucose consumption HepG2 Dual-specificity phosphatase-9

Antitumor Activities and Apoptosis-regulated Mechanisms of Fermented Wheat Germ Extract in the Transplantation Tumor Model of Human HT-29 Cells in Nude Mice

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Biomedical and Environmental Sciences 2015年第10期28卷 718-727页

作者： ZHANG Jia Yan XIAO Xiang DONG Ying WU Jing zhou xing hua School of Food and Biological Engineering Jiangsu University

Objective A subcutaneous transplantation tumor model of human HT-29 cells in nude mice was established to evaluate anticarcinogenic activities, and the apoptosis-regulated mechanism effect of aqueous extract of fermented wheat germ with Lactobacillus plantarum dy-1 （LFWGE）. Methods The HT-29 cells were transplanted via subcutaneous injection of 1×10^7cells into the right flank of each nude mouse. Then, nude mice were treated for 30 d with LFWGE （high-dose 2 g/kg/d; low-dose 1 g/kg/d） and for 7 d with 5-fluorouracil （5-FU, 25 mg/kg/d） by gavage and intraperitoneal injection, respectively. An inhibition of tumor growth was observed. Results Tumor volume and weights decreased significantly in both groups of nude mice treated with LFWGE. In addition, the cell apoptosis rate of the LFWGE group （2 g/kg/d, 60.2%＋4.4%; 1 g/kg/d, 58.6%＋6.9%） was significantly higher than that of the control group （11.5%＋1.6%） and 5-FU group （32.1%＋3.5%） as measured by the TUNEL assay. Moreover, the real-time fluorescent quantitative PCR and Western blot method further confirmed these enhancing apoptosis and growth inhibition effects. The involvement of LFWGE in inducing apoptosis was confirmed by the expression of Bax, Bcl-2, Caspase-3, and CyclinD1. Conclusion The results showed that LFWGE could induce subcutaneous transplantation tumor apoptosis in nude mice and could be as a natural nutrient supplements or chemopreventive agent in the treatment of human colon cancer.

关键词： Fermented wheat germ extract Nude mice Antitumor Apoptosis Western blot Human HT-29 cells

Antitumor Activities and Apoptosis-regulated Mechanisms of Fermented Barley Extract in the Transplantation Tumor Model of Human HT-29 Cells in Nude Mice

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Biomedical and Environmental Sciences 2017年第1期30卷 10-21页

作者： YAO Fang ZHANG Jia Yan XIAO Xiang DONG Ying zhou xing hua School of Food and Biological Engineering Jiangsu University Zhenjiang 212013 Jiangsu China Jiangsu Agri-animal Husbandry Vocational College Taizhou 225300 Jiangsu China

Objective A subcutaneous transplantation tumor model of human HT-29 cells was established in nude mice to study the anticarcinogenic activities and apoptosis-regulatory mechanistic effect of aqueous extract of fermented barley with Lactobacillus plantarum dy-1 （LFBE）. Methods HT-29 cells were transplanted via subcutaneous injection of 1 × 107cells into the right flank of each nude mouse. Then, nude mice were treated for 30 days with LFBE （high-dose 2 g·kg-1·d-1; low-dose 1 g·kg-1·d-1） and for 7 days with 5-fluorouracil （5-FU, 25 g·kg-1·d-1） by gavage and intraperitoneal injection, respectively. Results Tumor volume and weight decreased significantly in both groups of nude mice treated with LFBE. In addition, the cell apoptosis rate of the LFBE group was significantly higher than that of the control group and 5-FU groups as measured by the TUNEL assay. Moreover, the real-time fluorescent quantitative PCR and Western blot methods further confirmed these apoptosis-enhancing and growth-inhibiting effects. The involvement of LFBE in inducing apoptosis was confirmed by the expression of Bax, Bcl-2, caspase-3, and cyclin D1. Conclusion The results showed that LFBE could induce subcutaneous transplantation tumor apoptosis in nude mice and could be used as a natural nutrient supplement or chemopreventive agent in the treatment of human colon cancer.

关键词： Fermented barley extract Nude mice Antitumor Apoptosis Human HT-29 cells

Effects of Treatment with Different Combinations of Bisphenol Compounds on the Mortality of Caenorhabditis Elegans

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Biomedical and Environmental Sciences 2020年第2期33卷 145-149页

作者： Naomi HAMAMBA LI Jie ZHANG Xiao Wei ZHANG Cai Qin Frederick SARPONG zhou xing hua School of Food and Biological Engineering Jiangsu UniversityZhenjiang 212013Jiangsu China

Bisphenol A(BPA)is believed to affect a variety of physiological functions associated with human diseases[1].Bisphenol F(BPF)is an alternative to bisphenol *** percent of BPF is used as a monomer in the synthesis of polyvinyl chloride(PVC)and other compounds[2].Bisphenol S(BPS)is a structural analog of bisphenol A,which is widely used as a substitute in a variety of daily-use consumer goods and *** compound is often detected in indoor dust,urine,and human blood.

关键词： Effects Treatment Caenorhabditis

Determination of Fipronil and Its Metabolites in Eggs by Indirect Competitive ELISA and Lateral-flow Immunochromatographic Strip

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Biomedical and Environmental Sciences 2020年第9期33卷 731-734页

作者： zhou xing hua ZHANG Cai Qin ZHANG Xun SUN Cheng LI Jie XIAO Xiang ZHAO Yan Sheng OUYANG Qin WANG Yunu School of Food and Biological Engineering Jiangsu UniversityZhenjiang 212013JiangsuChina Jiangsu Meizheng Biological Technology Co.Ltd. Wuxi 214000JiangsuChina Jiangsu Jiangnan Biotech Co. Ltd.Zhenjiang 212013JiangsuChina

Fipronil is a phenylpyrrole insecticide that is widely used to control a variety of pests associated with crops in both agricultural and non-agricultural areas[1]. In the natural environment, fipronil can degrade to fipronil-desulfinyl, fipronil-sulfone, or fipronil-sulfide by photolysis, oxidation, or reduction,and these metabolites are more toxic to animal tissue than the parent molecule[2].

关键词： crops pests flow

茅盾文学批评中的“同化”与“顺应”——中国现代“主流”批评生成演化的微观分析

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求是学刊 2009年第1期36卷 108-113页

作者：周兴华浙江万里学院文化与传播学院浙江宁波315100

政治追求是贯穿于20世纪中国文学批评进程的一条鲜明线索。茅盾一生的文学批评活动恰好提供了中国现代"主流"批评生成演化的个人证词。在文学与政治、个人与集体、权力意志与个人思考之间,茅盾一直处在不断的同化与顺应过程中,他的批评... 详细信息

政治追求是贯穿于20世纪中国文学批评进程的一条鲜明线索。茅盾一生的文学批评活动恰好提供了中国现代"主流"批评生成演化的个人证词。在文学与政治、个人与集体、权力意志与个人思考之间,茅盾一直处在不断的同化与顺应过程中,他的批评成果不仅表现了一代知识分子不断选择、不断徘徊、不断矛盾的心路历程,也显现了"主流"批评发展的历史轨迹。

关键词： “主流”批评茅盾文学批评同化与顺应价值

结合遗传算法的LVQ神经网络在声学底质分类中的应用

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地球物理学报 2007年第1期50卷 313-319页

作者：唐秋华刘保华陈永奇周兴华丁继胜中国海洋大学海洋地球科学学院青岛266003 国家海洋局第一海洋研究所青岛266061

学习向量量化(Learning Vector Quantization,LVQ)神经网络在声学底质分类中具有广泛应用.常用的LVQ神经网络存在神经元未被充分利用以及算法对初值敏感的问题,影响底质分类精度.本文提出采用遗传算法(Genetic Algorithms,GA)优化神经... 详细信息

学习向量量化(Learning Vector Quantization,LVQ)神经网络在声学底质分类中具有广泛应用.常用的LVQ神经网络存在神经元未被充分利用以及算法对初值敏感的问题,影响底质分类精度.本文提出采用遗传算法(Genetic Algorithms,GA)优化神经网络的初始值,将GA与LVQ神经网络结合起来,迅速得到最佳的神经网络初始权值向量,实现对海底基岩、砾石、砂、细砂以及泥等底质类型的快速、准确识别.将其应用于青岛胶州湾海区底质分类识别研究中,通过与标准的LVQ神经网络的分类结果进行比较表明,该方法在分类速度以及精度上都有了较大提高.

关键词：学习向量量化遗传算法多波束测深系统底质分类

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运用大数据技术实现高校思想政治教育智能化

中南民族大学学报（人文社会科学版） 2023年第7期43卷 168-173,188页

作者：李英震周兴华大连民族大学信息与通信工程学院辽宁大连116600

大数据技术的发展为高校思想政治教育确立了全新的思维方式,提供了新时代的教育模式、方法和手段,利用大数据技术,可智能获取网络行为痕迹、智能描绘立体画像、智能构建教育资源数据库、智能精准推送教育内容,对于推进新时代高校思想政... 详细信息

大数据技术的发展为高校思想政治教育确立了全新的思维方式,提供了新时代的教育模式、方法和手段,利用大数据技术,可智能获取网络行为痕迹、智能描绘立体画像、智能构建教育资源数据库、智能精准推送教育内容,对于推进新时代高校思想政治教育创新发展具有重要作用。

关键词：大数据高校思想政治教育智能化

维普期刊数据库博看期刊

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有机太阳能电池的研究进展

合肥学院学报（自然科学版） 2009年第4期19卷 1-7页

作者：张剑锋 zhou hua-xing YOU Wei Department of Chemistry University of North Carolina at Chapel Hill Chapel Hill North Carolina 27599 USA 宁波大学材料科学与化学工程学院浙江宁波315211

回顾了近五年有机太阳能电池在电池材料和器件性能方面的研究进展.在材料方面,合成具有低带隙的化合物,使之与太阳光谱有更好的匹配,以提高对太阳光的吸收范围;在器件方面,通过使用异质结和纳米结构,使之在增加光吸收的同时保证激子的... 详细信息

回顾了近五年有机太阳能电池在电池材料和器件性能方面的研究进展.在材料方面,合成具有低带隙的化合物,使之与太阳光谱有更好的匹配,以提高对太阳光的吸收范围;在器件方面,通过使用异质结和纳米结构,使之在增加光吸收的同时保证激子的分离与有效迁移.有机聚合物太阳能电池是有机太阳能电池的发展方向,设计并合成具有低带宽和低HOMO能级的D-A型聚合物是提高其理论光能转化效率的关键.通过改进与提高电池的制作技术,充分挖掘新材料的潜力,将获得较大光能转换效率的有机太阳能电池.

关键词：太阳能电池光伏电池聚合物