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Journal of Environmental Sciences 2015年第8期27卷 212-218页

作者： Jennifer L.Morrell-Falvey James G.Elkins zhi-wu wang Bio Energy Science Center Biosciences Division Oak Ridge National Laboratory The Ohio State University ATI

This study took advantage of resorufin cellobioside as a fluorescent substrate to determine the distribution of cellulase activity in cellulosic biomass fermentation systems. Cellulolytic biofilms were found to express nearly four orders greater cellulase activity compared to planktonic cultures of Clostridium thermocellum and Caldicellulosiruptor obsidiansis, which can be primarily attributed to the high cell concentration and surface attachment. The formation of biofilms results in cellulases being secreted close to their substrates, which appears to be an energetically favorable stategy for insoluble substrate utilization. For the same reason,cellulases should be closely associated with the surfaces of suspended cell in soluble substrate-fed culture, which has been verified with cellobiose-fed cultures of C. thermocellum and C. obsidiansis. This study addressed the importance of cellulase activity distribution in cellulosic biomass fermentation, and provided theoretical foundation for the leading role of biofilm in cellulose degradation. System optimization and reactor designs that promote biofilm formation in cellulosic biomass hydrolysis may promise an improved cellulosic biofuel process.

关键词： Thermophilic Biofilm Cellulose Cellulase Biofuel

Coupling analysis of transcutaneous energy transfer coils with planar sandwich structure for a novel artificial anal sphincter

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Journal of Zhejiang University-Science C(Computers and Electronics) 2014年第11期15卷 1021-1034页

作者： Lei KE Guo-zheng YAN Sheng YAN zhi-wu wang Da-sheng LIU Institute of Medical Precision Engineering and Intelligent Systems School of Electronic Information and Electrical EngineeringShanghai Jiao Tong University

This paper presents a set of analytical expressions used to determine the coupling coefficient between primary and secondary Litz-wire planar coils used in a transcutaneous energy transfer(TET) system. A TET system has been designed to power a novel elastic scaling artificial anal sphincter system(ES-AASS) for treating severe fecal incontinence(FI), a condition that would benefit from an optimized TET. Expressions that describe the geometrical dimension dependence of self- and mutual inductances of planar coils on a ferrite substrate are provided. The effects of ferrite substrate conductivity, relative permeability, and geometrical dimensions are also considered. To verify these expressions, mutual coupling between planar coils is computed by 3D finite element analysis(FEA), and the proposed expressions show good agreement with numerical results. Different types of planar coils are fabricated with or without ferrite substrate. Measured results for each of the cases are compared with theoretical predictions and FEA solutions. The theoretical results and FEA results are in good agreement with the experimental data.

关键词： Transcutaneous energy transfer Planar spiral inductance Mutual inductance Coupling coefficient Artificial anal sphincter Fecal incontinence

Continuous live cell imaging of cellulose attachment by microbes under anaerobic and thermophilic conditions using confocal microscopy

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Journal of Environmental Sciences 2013年第5期25卷 849-856页

作者： zhi-wu wang Seung-Hwan Lee James G.Elkins Yongchao Li Scott Hamilton-Brehm Jennifer L.Morrell-Falvey BioEnergy Science Center Biosciences DivisionOak Ridge National LaboratoryOak RidgeTN 37831USA College of Food Agricultural and Environmental SciencesThe Ohio State University1328 Dover RdWoosterOH 44691USA National Institute of Advanced Industrial Science and Technology Biomass Refinery Research CenterHiroshimaJapan Department of Forest Biomaterials Engineering College of Forest and Environmental SciencesKangwon National UniversityChuncheon 200-701Korea

Live cell imaging methods provide important insights into the dynamics of cellular processes that cannot be derived easily from population-averaged *** the bioenergy field,much research is focused on fermentation of cellulosic biomass by thermophilic microbes to produce biofuels;however,little effort is dedicated to the development of imaging tools to monitor this dynamic biological *** is,in part,due to the experimental challenges of imaging cells under both anaerobic and thermophilic *** an imaging system is described that integrates confocal microscopy,a flow cell device,and a lipophilic dye to visualize *** to technical obstacles regarding suitable fluorescent markers,photodamage during imaging,and maintenance of environmental conditions during imaging are *** system was utilized to observe cellulose colonization by Clostridium thermocellum under anaerobic conditions at 60℃.This method enables live cell imaging of bacterial growth under anaerobic and thermophilic conditions and should be widely applicable to visualizing different cell types or processes in real time.

关键词： thermophile lipophilic dye cellulose biofuel confocal laser scanning microscopy biofilm

Overview of in-situ oxygen production technologies for lunar resources

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 233-255页

作者： Youpeng Xu Sheng Pang Liangwei Cong Guoyu Qian Dong wang Laishi Li Yusheng wu zhi wang College of Materials Science and Engineering Shenyang University of TechnologyShenyang 110870China CAS Key Laboratory of Green Process and Engineering National Engineering Research Center for Green Recycling of Strategic Metal ResourcesInstitute of Process EngineeringChinese Academy of SciencesBeijing 100190China Innovation Academy for Green Manufacture Chinese Academy of SciencesBeijing 100190China School of Chemical Engineering University of Chinese Academy of SciencesBeijing 100049China

The rich resources and unique environment of the Moon make it an ideal location for human expansion and the utilization of extraterrestrial ***,crucial for supporting human life on the Moon,can be extracted from lunar regolith,which is highly rich in oxygen and contains polymetallic *** oxygen and metal extraction can be achieved using existing metallurgical ***,the ample reserves of water ice on the Moon offer another means for oxygen *** paper offers a detailed overview of the leading technologies for achieving oxygen production on the Moon,drawing from an analysis of lunar resources and environmental *** delves into the principles,processes,advantages,and drawbacks of water-ice electrolysis,two-step oxygen production from lunar regolith,and one-step oxygen production from lunar *** two-step methods involve hydrogen reduction,carbothermal reduction,and hydrometallurgy,while the one-step methods encompass fluorination/chlorination,high-temperature decomposition,molten salt electrolysis,and molten regolith electrolysis(MOE).Following a thorough comparison of raw materials,equipment,technology,and economic viability,MOE is identified as the most promising approach for future in-situ oxygen production on the *** the corrosion characteristics of molten lunar regolith at high temperatures,along with the Moon's low-gravity environment,the development of inexpensive and stable inert anodes and electrolysis devices that can easily collect oxygen is critical for promoting MOE technology on the *** review significantly contributes to our understanding of in-situ oxygen production technologies on the Moon and supports upcoming lunar exploration initiatives.

关键词： lunar resources in-situ oxygen production space metallurgy molten lunar regolith electrolysis

Sustainable treatment of nitrate-containing wastewater by an autotrophic hydrogen-oxidizing bacterium

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Environmental Science and Ecotechnology 2022年第1期9卷 53-59页

作者： Yi-Zhen Chen Li-Juan Zhang Ling-Yun Ding Yao-Yu Zhang Xi-Song wang Xue-Jiao Qiao Bao-Zhu Pan zhi-wu wang Nan Xu Hu-Chun Tao Key Laboratory for Heavy Metal Pollution Control and Reutilization School of Environment and EnergyPeking University Shenzhen Graduate SchoolShenzhen518055GuangdongChina College of Health Science and Environmental Engineering Shenzhen Technology UniversityShenzhen518118GuangdongChina State Key Laboratory of Eco-hydraulic in Northwest Arid Region of China Xi'an University of TechnologyXi'an710048ShaanxiChina Department of Civil and Environmental Engineering Virginia Polytechnic Institute and State UniversityManassas20147VirginiaUSA

Bacteria are key denitrifiers in the reduction of nitrate(NO_(3)^(-)N),which is a contaminant in wastewater treatment plants(WWTPs).They can also produce carbon dioxide(CO_(2))and nitrous oxide(N2O).In this study,the autotrophic hydrogen-oxidizing bacterium Rhodoblastus ***_(2)0 was isolated for sustainable treatment of NO_(3)^(-)N in *** removal of NO_(3)^(-)N and recovery of biomass nitrogen were *** to 99%of NO_(3)^(-)N was removed without accumulation of nitrite and N2O,consuming CO_(2)of 3.25 mol for each mole of NO_(3)^(-)N *** overall removal rate of NO_(3)^(-)N reached 1.1 mg L^(-1)h^(-1)with a biomass content of approximately 0.71 g L^(-1)within 72 ***20 participated in NO_(3)^(-)N assimilation and aerobic *** from 15N-labeled-nitrate test indicated that removed NO_(3)^(-)N was assimilated into organic nitrogen,showing an assimilation efficiency of 58%.Seventeen amino acids were detected,accounting for 43%of the *** loss through aerobic denitrification was only approximately 42%of total *** study suggests that TH_(2)0 can be applied in WWTP facilities for water purification and production of valuable biomass to mitigate CO_(2)and N_(2)O emissions。

关键词： Wastewater Nitrate Hydrogen-oxidizing bacteria Autotrophic assimilation Aerobic denitrification

Repetitive transcranial magnetic stimulation in Alzheimer’s disease:effects on neural and synaptic rehabilitation

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Neural Regeneration Research 2025年第2期20卷 326-342页

作者： Yi Ji Chaoyi Yang Xuerui Pang Yibing Yan Yue wu zhi Geng Wenjie Hu Panpan Hu Xingqi wu Kai wang Department of Neurology The First Affiliated Hospital of Anhui Medical UniversityHefeiAnhui ProvinceChina Anhui Province Key Laboratory of Cognition and Neuropsychiatric Disorders HefeiAnhui ProvinceChina Collaborative Innovation Center of Neuropsychiatric Disorders and Mental Health HefeiAnhui ProvinceChina Institute of Artificial Intelligence Hefei Comprehensive National Science CenterHefeiAnhui ProvinceChina Department of Psychology and Sleep Medicine The Second Affiliated Hospital of Anhui Medical UniversityHefeiAnhui ProvinceChina

Alzheimer’s disease is a neurodegenerative disease resulting from deficits in synaptic transmission and *** Alzheimer’s disease brain tends to be hyperexcitable and hypersynchronized,thereby causing neurodegeneration and ultimately disrupting the operational abilities in daily life,leaving patients *** transcranial magnetic stimulation is a cost-effective,neuro-modulatory technique used for multiple neurological *** the past two decades,it has been widely used to predict cognitive decline;identify pathophysiological markers;promote neuroplasticity;and assess brain excitability,plasticity,and *** has also been applied to patients with dementia,because it can yield facilitatory effects on cognition and promote brain recovery after a neurological ***,its therapeutic effectiveness at the molecular and synaptic levels has not been elucidated because of a limited number of *** study aimed to characterize the neurobiological changes following repetitive transcranial magnetic stimulation treatment,evaluate its effects on synaptic plasticity,and identify the associated *** review essentially focuses on changes in the pathology,amyloidogenesis,and clearance pathways,given that amyloid deposition is a major hypothesis in the pathogenesis of Alzheimer’s *** mechanisms associated with repetitive transcranial magnetic stimulation procedures and different pathways mediating gene transcription,which are closely related to the neural regeneration process,are also ***,we discuss the outcomes of animal studies in which neuroplasticity is modulated and assessed at the structural and functional levels by using repetitive transcranial magnetic stimulation,with the aim to highlight future directions for better clinical translations.

关键词： Alzheimer’s disease amyloid deposition apoptotic mechanisms biomarker neural regeneration neurodegeneration repetitive transcranial magnetic stimulation synaptic plasticity

The Inventory for Chinese LFC in 2006

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China Foundry 2007年第3期4卷 240-242页

作者： YE Sheng-ping wu zhi-chao wang Lei National Key Mold Laboratory of Huazhong University of Science and Technology Wuhan 430074 P. R. China

1. Introduction Lost Foam Casting has been introduced into China for two decades, and it is getting more popularity in foundry industry. In China there are two associations responsible for the LFC industry and academic exchanges： Chinese Lost Foam Casting Society （CLFCS） and Chinese Association of Full Mold Casting. Each of the associations has hundreds of members respectively. In 2006, the 7th Lost Foam Casting Symposium organized by CLFCS was grandly held in Nanjing University of Aeronautics and Astronautics, and 245 domestic delegates and 14 foreign experts from USA, Canada, Germany,

关键词：中国铸造行业铸造工艺泡沫

“One stone, two birds”: Salt template enabling porosity engineering and single metal atom coordinating toward high-performance zinc-ion capacitors

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Journal of Energy Chemistry 2025年第1期100卷 637-645页

作者： Chunliu Zhu Huanyu Liang Ping Li Chenglong Qiu Jingyi wu Jingwei Chen Weiqian Tian Yue Zhu zhi Li Huanlei wang School of Materials Science and Engineering Ocean University of China Department of Chemical and Materials Engineering University of Alberta

Zinc-ion hybrid capacitors （ZIHCs） have received increasing attention as energy storage devices owing to their low cost,high safety,and environmental ***,their progress has been hampered by low energy and power density,as well as unsatisfactory long-cycle stability,mainly due to the lack of suitable electrode *** this context,we have developed manganese single atoms implanted in nitrogen-doped porous carbon nanosheets （MnSAs/NCNs） using a metal salt template method as cathodes for *** metal salt serves a dual purpose in the synthesis process:It facilitates the uniform dispersion of Mn atoms within the carbon matrix and acts as an activating agent to create the porous *** applied in ZIHCs,the MnSAs/NCNs electrode demonstrates exceptional performance,including a high capacity of 203 m Ah g-1,an energy density of 138 Wh kg-1at 68 W kg-1,and excellent cycle stability with 91%retention over 10,000 *** calculations indicate that the introduced Mn atoms modulate the local charge distribution of carbon materials,thereby improving the electrochemical *** work demonstrates the significant potential of carbon materials with metal atoms in zinc-ion hybrid capacitors,not only in enhancing electrochemical performance but also in providing new insights and methods for developing high-performance energy storage devices.

关键词： Zinc ion hybrid capacitors Cathodes Carbon materials Heteroatoms Single atoms

Photoinduced radical sulfur dioxide insertion with asymmetric cyclization of alkenes: accessing β-chiral sulfones bearing S-stereocentric cyclic sulfinamides

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Science China Chemistry 2025年第1期68卷 257-263页

作者： Jiapian Huang Chenxin wang Xu wang Xiaoyu Zhou Wei Xiao Jie wu School of Pharmaceutical and Chemical Engineering & Institute for Advanced Studies Taizhou University State Key Laboratory of Organometallic Chemistry Shanghai Institute of Organic Chemistry Chinese Academy of Sciences School of Chemistry and Chemical Engineering Henan Normal University

The generation of various β-chiral sulfones bearing chiral cyclic sulfinamides through photoredox-catalyzed sulfur dioxide insertion with asymmetric radical cyclization is described. This method features a broad substrate scope and excellent stereospecificity. Computational studies reveal that the dispersion interaction between sulfinyl group and CH2SO2Ph group plays a crucial role in the stereoselectivity control.

关键词： β-chiral sulfones chiral cyclic sulfinamides sulfur dioxide insertion asymmetric radical cyclization stereoselectivity

A New Approach to Prepare 2-Acetyl-Mono(5,6,7,8)-Nitro-l,2,3,4-Tetrahydroisoquinolincs

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Chinese Chemical Letters 2000年第10期11卷 857-860页

作者： Chong Zhao RAN, Tat zhi wu, Guo Ping wang, Met Hua XIE (Department of Medicinal Chemistry, Shanghai institute of Pharmaceutical industry, Shanghai 200437)

N-Acetyl-P. m or o-nitro-phenylethylamines and (HCHO). were treated in 60% H,SO./HOAc. via α -amidoalkylation to give 2-acetyl-mono(5, 6, 7 or 8)-nitro-l .2,3.4- tetrahydroisoquinolines. Additionally. solme interesting phenomena were observed when the comparison between 2-acetyl-5, 6. 7 or 8-nitro-l,2.3.4-tetrahydroisoqui and 2-alkylsulfonyl-5. 6 or 7nitro-l,2.3,4-tetrahydroisoquinolines was made.

关键词： α-Amidoalkylation 2-acetyl-mono (5 6. 7. 8)-nitro-l.2,3.4-tetrahydroisoqu nitro- I .2.3.4-tetrahydroisoquinolines.