Continuous live cell imaging of cellulose attachment by microbes under anaerobic and thermophilic conditions using confocal microscopy

作     者：Zhi-Wu Wang Seung-Hwan Lee James G.Elkins Yongchao Li Scott Hamilton-Brehm Jennifer L.Morrell-Falvey 

作者机构：BioEnergy Science CenterBiosciences DivisionOak Ridge National LaboratoryOak RidgeTN 37831USA College of FoodAgricultural and Environmental SciencesThe Ohio State University1328 Dover RdWoosterOH 44691USA National Institute of Advanced Industrial Science and TechnologyBiomass Refinery Research CenterHiroshimaJapan Department of Forest Biomaterials EngineeringCollege of Forest and Environmental SciencesKangwon National UniversityChuncheon 200-701Korea 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2013年第25卷第5期

页      面：849-856页

核心收录：

学科分类：0830[工学-环境科学与工程（可授工学、理学、农学学位）] 081702[工学-化学工艺] 08[工学] 0817[工学-化学工程与技术] 

基　　金：supported by the BioEnergy Science Center (BESC) which is a U.S. Department of Energy Bioenergy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science 

主　　题：thermophile lipophilic dye cellulose biofuel confocal laser scanning microscopy biofilm 

摘      要：Live cell imaging methods provide important insights into the dynamics of cellular processes that cannot be derived easily from population-averaged *** the bioenergy field,much research is focused on fermentation of cellulosic biomass by thermophilic microbes to produce biofuels;however,little effort is dedicated to the development of imaging tools to monitor this dynamic biological *** is,in part,due to the experimental challenges of imaging cells under both anaerobic and thermophilic *** an imaging system is described that integrates confocal microscopy,a flow cell device,and a lipophilic dye to visualize *** to technical obstacles regarding suitable fluorescent markers,photodamage during imaging,and maintenance of environmental conditions during imaging are *** system was utilized to observe cellulose colonization by Clostridium thermocellum under anaerobic conditions at 60℃.This method enables live cell imaging of bacterial growth under anaerobic and thermophilic conditions and should be widely applicable to visualizing different cell types or processes in real time.