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Skp2-RNAi suppresses proliferation and migration of gallbladder carcinoma cells by enhancing p27 expression

World Journal of Gastroenterology 2013年第30期19卷 4917-4924页

作者： Bin Zhang Lin-Hua ji Wei Liu gang Zhao zhi-Yong Wu Department of General Surgery Renji HospitalShanghai Jiaotong University School of Medicine

AIM:To explore the role of S-phase kinase-associated protein-2(Skp2)in gallbladder carcinoma and to identify whether depletion of Skp2 by Skp2-RNAi could attenuate proliferation and migration of gallbladder ***:Skp2-RNAi was transduced into cells of the gallbladder carcinoma cell line GBC-SD,using a lentiviral *** effect of Skp2-RNAi on the proliferation,migration,invasion and cell cycle of GBC-SD cells was studied using in vitro assays for cell proliferation,colony formation,wound healing and cell *** expression of Skp2 and p27 was detected by real-time polymerase chain reaction and Western *** effect of Skp2-RNAi on the proliferation of GBC-SD cells in vivo was investigated by tumorigenicity experiments in nude ***:Lentivirus-mediated RNAi reduced the expression of Skp2 in cultured *** expression of the p27 protein increased along with the down-regulation of Skp2,although no significant difference was found in p27 mRNA *** cytometry revealed that Skp2-RNAi transfection significantly increased the proportion of cells in the S phase and significantly decreased the proportion of cells in the G 2 /M *** significant difference in the frequency of cells in the G0/G1 phase was *** results from the cell proliferation,colony formation and wound healing assays revealed that Skp2-RNAi transfection markedly inhibited the proliferation and migration of GBC-SD cells in ***,tumorigenicity experiments showed that suppression of Skp2 significantly decreased the weights of the tumors(0.56 ± 0.11 and 0.55 ± 0.07 g in the control and Scr-RNAi groups vs 0.37 ± 0.09 and 0.35 ± 0.08 g in the Skp2-RNAi-L and Skp2-RNAi-H groups).CONCLUSION:The expression of Skp2 in GBC-SD cells was inhibited following Skp2-RNAi *** of the Skp2 gene inhibited proliferation,migration and invasiveness of GBC-SD cells by mechanisms dependent on enhanced expression of the p27 protein.

关键词： Gallbladder carcinoma S-phase kinaseassociated protein-2 p27 Gene therapy Cell cycle

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CELL AND MOLECULAR BIOMECHANICS: PERSPECTIVES AND CHALLENGES

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Acta Mechanica Solida Sinica 2011年第1期24卷 27-51页

作者： Baohua ji gang Bao Department of Applied Mechanics Beijing Institute of Technology Beijing 100081 China Department of Biomedical Engineering Georgia Institute of Technology and Emory University Atlanta GA 30332 USA

As an intriguing interdisciplinary research field, cell and molecular biomechanics is at the cutting edge of mechanics in general and biomechanics in particular. It has the potential to provide a quantitative understanding of how forces and deformation at tissue, cellular and molecular levels affect human health and disease. In this article, we review the recent advances in cell and molecular biomechanics, examine the available computational and experimental tools, and discuss important issues including protein deformation in mechanotransduction, cell deformation and constitutive behavior, cell adhesion and migration, and the associated models and theories. The opportunities and challenges in cell and molecular biomechanics are also discussed. We hope to provide readers a clear picture of the current status of this field, and to stimulate a broader interest in the applied mechanics community.

关键词： molecular biomechanics cell mechanics protein mechanics cell adhesion cell migration mechanotransduction

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涉及跟骨关节面的粉碎性骨折的手术治疗

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实用骨科杂志 2010年第3期16卷 184-186页

作者：白志刚聂继平李昶马志新内蒙古医学院第二附属医院手足外科内蒙古呼和浩特010030

目的探讨应用可塑性解剖型钛钢板内固定治疗累及跟骨后距关节面粉碎性骨折的疗效。方法83例96个足跟骨后关节面粉碎性骨折患者,均采用切开复位可塑性解剖钛钢板固定。其中急诊手术44足,择期手术52足。术后随访时间18～36个月。结果近期... 详细信息

目的探讨应用可塑性解剖型钛钢板内固定治疗累及跟骨后距关节面粉碎性骨折的疗效。方法83例96个足跟骨后关节面粉碎性骨折患者,均采用切开复位可塑性解剖钛钢板固定。其中急诊手术44足,择期手术52足。术后随访时间18～36个月。结果近期并发皮缘部分坏死9足,其中急诊手术发生4足,发生率9.1%(4/44);择期手术组发生5足,发生率9.6%(5/52)。远期治疗效果按美国骨科协会足踝外科分会(AOFAS)踝后足评分标准进行功能评分,优54足,良26足,可16足,优良率83.3%(80/96)。经统计学分析急诊手术与择期手术的治疗效果比较无统计学意义(P>0.05),但在不同骨折类型之间治疗结果的差异有统计学意义(P<0.05)。结论可塑性解剖钛钢板内固定是一种治疗跟骨后距关节面粉碎性骨折的有效方法,急诊手术与择期手术比较对疗效无影响,骨折类型是影响疗效的重要因素。

关键词：跟骨骨折骨折固定术解剖钛板

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Oxygen content and magnetic properties of composites La_(0.75)Sr_(0.25)MnO_(3±δ) dcalcined at different temperatures

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Rare Metals 2014年第4期33卷 452-458页

作者： Deng-Hui ji Xue Hou Gui-De Tang Zhuang-zhi Li Deng-Lu Hou Ming-gang Zhu Hebei Advanced Thin Films Laboratory Department of PhysicsHebei Normal University Division of Functional Materials Research Central Iron & Steel Research Institute

The effects of the calcination temperature on the oxygen content and magnetic properties of the nano- crystalline perovskite manganite Lao.75Sro.25MnO3±δ pre- pared by the sol-gel method were investigated. The highest temperatures at which the samples were calcined were 973, 1073, 1273, and 1473 K. The X-ray diffraction （XRD） analyses indicate that all the samples have only a single phase with the R3c perovskite structure. As the calcination temperature and calcination time increase, the oxygen content in the samples increases from being deficient to being in excess of that in the stoichiometric formula. The crystallite size also increases from 23 to 283 nm. Magnetic measurements indicate that the sample calcined at 1073 K has the highest Curie temperature. This is owing to the fact that the crystallite cores of this sample have stoichiometric oxygen content. The dependence of the Curie temperature and the saturation magnetization on the calcination tem- perature are successfully explained.

关键词： Perovskite manganite Magnetic property Oxygen content Sol-gel method

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Smooth particle hydrodynamics modeling of cutting force in milling process of TC4

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Advances in Manufacturing 2019年第4期7卷 364-373页

作者： Xiao-Guang Guo Ming Li zhi-gang Dong Rui-Feng Zhai Zhu-ji jin Ren-Ke Kang Key Laboratory for Precision and Non-traditional Machining Technology of Ministry of Education Dalian University of TechnologyDalian 116024LiaoningPeople's Republic of China

Milling is one of the main methods for processing titanium *** present,the complex process of milling is usually simulated by finite element method,which often has problems in mesh distortion and mesh ***,a meshless three-dimensional milling simulation model was established for TC4 titanium alloy using the smooth particle hydrodynamics(SPH)***,the established SPH model was analyzed by the LS-DYNA software,and the stress distribution,temperature field,and cutting force during milling were studied under specific ***,the cutting force was simulated under different cutting parameters and the effects of these parameters on the cutting force were ***,based on a series of cutting force experiments,the accuracy of the simulation model was *** study proves the feasibility of SPH method in the simulation of titanium alloy milling process and provides novel methods for investigating the processing mechanism and optimizing the processing technology of titanium alloys.

关键词： TC4 titanium Smooth particle hydrodynamics(SPH)-Milling Cutting temperature Cutting force

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Deep forest

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National Science Review 2019年第1期6卷 74-86页

作者： zhi-Hua Zhou ji Feng National Key Laboratory for Novel Software Technology Nanjing University

Current deep-learning models are mostly built upon neural networks, i.e. multiple layers of parameterized differentiable non-linear modules that can be trained by backpropagation. In this paper, we explore the possibility of building deep models based on non-differentiable modules such as decision trees. After a discussion about the mystery behind deep neural networks, particularly by contrasting them with shallow neural networks and traditional machine-learning techniques such as decision trees and boosting machines,we conjecture that the success of deep neural networks owes much to three characteristics, ***-by-layer processing, in-model feature transformation and sufficient model complexity. On one hand,our conjecture may offer inspiration for theoretical understanding of deep learning; on the other hand, to verify the conjecture, we propose an approach that generates deep forest holding these characteristics. This is a decision-tree ensemble approach, with fewer hyper-parameters than deep neural networks, and its model complexity can be automatically determined in a data-dependent way. Experiments show that its performance is quite robust to hyper-parameter settings, such that in most cases, even across different data from different domains, it is able to achieve excellent performance by using the same default setting. This study opens the door to deep learning based on non-differentiable modules without gradient-based adjustment, and exhibits the possibility of constructing deep models without backpropagation.

关键词： deep forest deep learning machine learning ensemble methods decision trees

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Mycoplasma leachii causes polyarthritis in calves via the blood route but is not associated with pneumonia

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Journal of Integrative Agriculture 2018年第11期17卷 2536-2545页

作者： CHANG ji-tao WANG Guan-bo ZHANG Yue WANG Fang jiANG zhi-gang YU Li Division of Livestock Infectious Diseases/State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research InstituteChinese Academy of Agricultural Sciences

Mycoplasma leachiiwas initially isolated from arthritic calves in South Queensland, Australia, and its ability to cause clinical polyarthritis in calves was demonstrated by experimental infection. However, the source of M. leachii infection in calves and its means of spreading are not well known. In this study, one-month-old calves were inoculated with cultures of M. leachii or joint fluid （collected from M. leachii-infected calves） through the intraarticular, intravenous, intratracheal, intranasal or oral routes. Multidisciplinary procedures, including clinical assessment, etiology assessment, pathology and immunohistochemistry （IHC）, were used to evaluate the pathogenicity of M. leachfi in calves and to elucidate the transmission route of M. leachii infection in calves. The results showed that all calves inoculated intraarticularly with cultured GN407 or joint fluid and two-thirds of the calves inoculated intravenously with joint fluid developed severe polyarthritis, and the M. leachfi antigen was detected in the joints of all infected calves by IHC and PCR. In contrast, calves inoculated with cultured M. leachfi or joint fluid through the intratracheal, intranasal or oral routes did not show any M. leachfi infection in the clinical assessment, etiology assessment, or pathology and IHC results. These results indicated that polyarthritis caused by M. leachii in calves is transmitted via the blood route; however, this disease is not transmitted through the respiratory or digestive routes. In addition, the M. leachii antigen was not detected in the lungs of all the inoculated calves using IHC and PCR, indicating that M. leachfi is not associated with pneumonia, even in the calves inoculated through the respiratory duct. These findings are important information for the prevention and control of calf polyarthritis caused by M. leachfi.

关键词： Mycoplasma leachii calf polyarthritis transmission via blood

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Effect of Dy substitution on the microstructure and magnetic properties of nanograin Nd-Fe-B single-phase alloys

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International Journal of Minerals,Metallurgy and Materials 2010年第3期17卷 335-339页

作者： zhi-an Chen ji Luo zhi-meng Guo School of Materials Science and Engineering University of Science and Technology Beijing Beijing 100083 China

Nanocrystalline single-phase alloys with the nominal compositions （at%） of Nd12.3-xDyxFe79.7Zr0.8Nb0.8Cu0.4B6.0 （x=0, 0.5, 1.5, and 2.5） were prepared by melt-spinning and subsequent annealing. X-ray diffraction analysis shows that the as-spun ribbons were mainly com- posed of the amorphous phase. A slight content of Dy stabilizes the amorphous phase during annealing treatment. The grain size becomes smaller and the coercivity of the annealed ribbon is gradually improved with the increase of Dy content. Excessive Dy is harmful to the remanence. It is found that no intergramtlar phase exists between the grains by high-resolution transmission electron microscopy, and the grain boundaries are crystallographically coherent in the optimally annealed sample. The optimum magnetic properties of remanence （Jr=1.09 T）, coercivity （Hei=1048 kA/m）, and maximum magnetic energy product （（BH）m=169.5 kJ/m3） are obtained from the x=0.5 ribbon in a post heat-treated state （700℃, 10 min）.

关键词： NdFeB magnets dysprosium nanocrystalline amorphous grain size magnetic properties

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A Novel Controllable Hybrid-Anode AlGaN/GaN Field-Effect Rectifier with Low Operation Voltage

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Chinese Physics Letters 2012年第10期29卷 174-177页

作者： WANG zhi-gang CHEN Wan-Jun ZHANG Bo LI Zhao-ji State Key Laboratory of Electronic Thin Films and Integrated Devices University of Electronic Science and Technology of ChinaChengdu 610054

A novel controllable hybrid-anode AlGaN/GaN field-effect rectifier(HA-FER)with low operation voltage(LOV)is *** mechanism can be explained by the field-controlled energy band *** model reveals that the electric field in the AlGaN layer alters the energy band to result in a variation of the two-dimensional electron gas(2DEG)at AlGaN/GaN interface;the field can be changed by the thickness d of the AlGaN layer and the applied *** the d reduces below the critical thickness,the 2DEG vanishes and then the channel is pinched ***,the threshold voltage of HA-FER can be designed as low as 0 V leading to LOV(<1 V).The analytical characteristic of the HA-FER is calculated and validated by the simulated *** results also demonstrate that the forward properties of HA-FER are superior to the conventional SBD due to the high Schottky barrier.

关键词： AlGaN/GaN AlGaN Schottky

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Onboard real time modeling of aircraft engines with Hammerstein-Wiener representation

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航空动力学报 2014年第10期29卷 2499-2506页

作者： WANG ji-qiang YE zhi-feng HU Zhong-zhi jiangsu Province Key Laboratory of Aerospace Power System College of Energy and Power EngineeringNanjing University of Aeronautics and Astronautics

An identification-based approach for aircraft engine modeling using the nonlinear HammersteinWiener representation was ***-Wiener modeling for both limited flight envelope and extended flight envelope was *** shows that the resulting model can be valid over 10%variation of rotational speed of the engine,compared with those linear models that are only valid over 3%—5%change of rotational *** is further demonstrated that the proposed method can be utilized over large envelope up to 20% variation of rotational speed of the *** fundamental idea is to use nonlinear models to extend the feasible/valid region rather than those linear *** may consequently simplify the switching logic in the onboard digital control *** is often overlooked in aircraft engine control community,but has been emphasized in the research.

关键词： aircraft engines engine modeling onboard real time modeling system identification Hammerstein-Wiener representation

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