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3D digital image microscope system-assisted vasovasostomy and vasoepididymostomy in rats

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Asian Journal of Andrology 2021年第4期23卷 396-399页

作者： Peng Li Na-Chuan Liu Er-Lei zhi Chen-Cheng Yao zhi-Liang Zhao zhi-yong yu Qi-Meng Li yu-Hua Huang Jie-Chang Ju Wen-Bin Huang Husanjan Rozi zhi-yong Ji San-Wei Guo Ru-Hui Tian Zheng Li Shanghai General Hospital Shanghai Jiao Tong University School of MedicineShanghai 200080China Gansu Provincial Hospital of Traditional Chinese Medicine Lanzhou 730050China Weifang Traditional Chinese Hospital Weifang 250200China The Third Affiliated Hospital of Wenzhou Medical University Wenzhou 325000China Zhengzhou Central Hospital Affiliated with Zhengzhou University Zhengzhou 450007China Wenzhou Integrated Traditional Chinese Wenzhou 325000China Kashgar District Second People's Hospital Kashgar 844000China Shanghai General Hospital of Nanjing Medical University Shanghai 200080China Shanghai Tenth People's Hospital Tongji UniversityShanghai 200072China

Optimal vision and ergonomics are essential factors contributing to the achievement of good results during *** three-dimensional(3D)digital image microscope system with a better 3D depth of field can release strain on the surgeon's neck and back,which can improve outcomes in *** report a randomized prospective study of vasoepididymostomy and vasovasostomy using a 3D digital image microscope system(3D-DIM)in rats.A total of 16 adult male rats were randomly divided into two groups of 8 each:the standard operating microscope(SOM)group and the 3D-DIM *** outcomes measured included the operative time,real-time postoperative mechanical patency,and anastomosis ***,a user-friendly microscope score was designed to evaluate the ergonomic design and equipment characteristics of the *** were no differences in operative time between the two *** real-time postoperative mechanical patency rates were 100.0%for both *** percentage of vasoepididymostomy anastomosis leakage was 16.7%in the SOM group and 25.0%in the 3D-DIM group;however,no vasovasostomy anastomosis leakage was found in either *** terms of the ergonomic design,the 3D-DIM group obtained better scores based on the surgeon's feelings;in terms of the equipment characteristics,the 3D-DIM group had lower scores for clarity and higher scores for flexibility and *** on our randomized prospective study in a rat model,we believe that the 3D-DIM can improve surgeon comfort without compromising outcomes in male infertility reconstructive microsurgery,so the 3D-DIM might be widely used in the future.

关键词： microsurgery three-dimensional digital image microscope system vasoepididymostomy vasovasostomy

Use of packed-fiber solid-phase extraction for sample clean-up and preconcentration of vitamin B_(12) before determination

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Chinese Chemical Letters 2009年第12期20卷 1491-1494页

作者： Fang Fang Xue Jun Kang zhi yong Liu yu Qin Ma Zhong Ze GU State Key Laboratory of Bioelectronics Southeast University Nanjing 210096 China

A rapid and simple preconcentration step applying packed-fiber solid-phase extraction columns has been investigated to vitamin B12. The extraction performance of the new method was investigated preliminarily on vitamin functional drink. The analysis used a reversed-phase C18 column, with a photo-diode array detector at 220 nm. The samples were preconcentrated with packed-fiber solid-phase extraction columns. Good linearity was observed in vitamin functional drink. The repeatability of extraction performance, expressed as relative standard deviations, was from 3.5% to 4.3%. The limit of detection （LOD） is 5 ng mL^-1 （S/N = 3）. Finally, the method had been applied for the determination of vitamin B12 in vitamin functional drink.

关键词： Vitamin B12 Nanofibers Packed-fiber solid-phase extraction Preconcentration

Impact of Soil Fumigation Practices on Soil Nematodes and Microbial Biomass

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Pedosphere 2004年第3期14卷 387-393页

作者： CAO zhi—Ping yu yong—Li CHEN Guo-Kang R.DAWSON Department of Ecology and Ecological Engineering College of Resources and Environmental ScienceChina Agricultural UniversityBeijing 100094(China).

This study was designed to understand the impact of methyl bromide (MB) (CH3Br) and its alternatives on both free-living and root-knot nematodes in the soil. A randomized complete block experiment with six treatments and 4 replicates (each replicate in a separate greenhouse) was established in Qingzhou, Shandong Province, China. In addition to MB and untreated control (CK) treatments there were four alternative soil fumigation practices including MB+virtually impermeable films (VIF), metam sodium (MS), MS +VIF and soil solarization combined with selected biological control agents (SS+BCA). Two tomato (Lycopersicum esculentum Mill.) cultivars, cv. Maofen-802 from the Xian Institute of Vegetable Science, China, and cv. AF179 Brillante from the Israeli Hazera Quality Seeds, were selected as test crops. The results indicated that Rhabditidae was the most dominant population with percentage abundance as high as 85% of the total number of identified free-living nematodes, followed by that of Cephalobidae. Methyl bromide and its alternatives except for the non-chemical SS+BCA treatment controlled the target pest, root-knot nematodes. Also, the impact of the three chemical alternatives on free-living nematode number and functional group abundance was similar to the impact associated with a typical methyl bromide application. Chemical fumigation practices, especially that with MB, significantly reduced the number of nematodes in the soil and simultaneously significantly reduced the number of nematode genera thereby reducing nematode diversity. All the four soil chemical fumigation activities decreased soil microbial biomass and had an obvious initial impact on microorganism biomass. Furthermore, both plant-parasitic and fungivore nematodes were positively correlated with soil microbial biomass.

关键词： methyl bromide microbial biomass nematode soil fumigation

Rare isolated extra-hepatic bile duct injury: A case report

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World Journal of Clinical Cases 2021年第20期9卷 5661-5667页

作者： Jian Zhao Yan-Li Dang Jia-Ming Lin Chun-Hai Hu zhi-yong yu Department of Hepatobiliary Surgery The Affiliated Hospital of Yunnan University(The Second People's Hospital of Yunnan Province)Kunming 650000Yunnan ProvinceChina Department of Obstetrics The First People's Hospital of Yunnan ProvinceKunming 650000Yunnan ProvinceChina

BACKGROUND Extra-hepatic bile duct injury(EHBDI)is very rare among all blunt abdominal *** to literature statistics,it only accounts for 3%-5%of abdominal injuries,most of which are combined *** EHBDI is more rare,with a special injury mechanism,clinical presentation and treatment strategy,so missed diagnosis easily *** SUMMARY We report a case of unexplained abdominal effusion and jaundice following blunt abdominal trauma in our *** which,surgical exploration of the case was performed and a large amount of bile leakage in the abdominal cavity was *** obvious abdominal organ damage or bile duct rupture was *** was terminated after the common bile duct indwelled with a T *** 2 wk,a T-tube angiography revealed the lesion in the common bile duct pancreatic segment,confirming isolated *** 2 mo later,the T tube was pulled out with re-examined magnetic resonance cholangiopancreatography,indicating narrowing of the common bile duct injury,with no special treatment due to no clinical symptoms and no abnormality in the current *** This case was featured by intraoperative bile leakage and no *** type of rare isolated EHBDI is prone to missed and delayed diagnosis due to its atypical clinical manifestations and imaging *** is still the main treatment,and the indications and principles of bile duct injury repair must be followed.

关键词： Bile duct Injury Blunt Abdominal trauma Diagnosis Case report

Risk factors of choledocholithiasis formation after liver transplantation

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Hepatobiliary & Pancreatic Diseases International 2013年第2期12卷 215-217页

作者： zhi-yong yu Min Zhang yun-Sheng Qin Xiao-Ping Zhou Ming-yue Cai Song-Feng yu Qing-Hong Ke Shu-Sen Zheng Division of Hepatobiliary and Pancreatic Surgery Key Laboratory of Combined Multi-organ Transplantation Ministry of Public Health and Key Laboratory of Organ Transplantation Zhejiang Province First Affiliated Hospital Zhejiang University School of Medicine

Systematic study of risk factors for biliary stone post-liver transplantation is rarely performed. To investigate the risk factor of choledocholithiasis formation after liver transplantation, we c onducted a case-control study. Fourteen patients were selected into a study group. The stones of the bile duct of the patients were confirmed and treated successfully by endoscopic retrograde cholangiopancreatography. For univariate analysis, we selected carefully some potential risk factors such as cold ischemia time, warm ischemia time, and biliary stricture. The results revealed that cold ischemia time and biliary stenosis were significant predictors. But multivariate analysis revealed that only biliary stenosis was a significant risk factor. In conclusion, biliary stenosis is a risk factor of bile duct stones formation after liver transplantation. Endoscopic retrograde cholangiopancreatography is effective and safe in the diagnosis or treatment of bile duct stones after liver transplantation.

关键词： liver transplantation endoscopic retrograde cholangiopancreatography choledocholithiasis biliary tract diseases common bile duct stone gallstones

Diagnostic value of contrast-enhanced intraoperative Doppler sonography for cerebral arteriovenous malformations compared with angiography

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Chinese Medical Journal 2010年第20期123卷 2812-2815页

作者： XU Hong-zhi QIN zhi-yong GU yu-xiang ZHOU Ping CHEN Xian-cheng Department of Neurosurgery Huashan HospitalFudan University Shanghai Neurosurgical Center Shanghai 200040China

Background Intraoperative Doppler sonography has been used in the neurosurgical operating room for the localization and description of cerebral arteriovenous malformations （AVMs）. This study aimed to investigate the clinical value of contrast-enhanced intraoperative Doppler sonography, including its ability to assess the location and identify of feeding arteries in patients with AVMs and to compare this method with *** Twenty-three patients with cerebral AVMs who were diagnosed using angiography, were examined with contrast-enhanced intraoperative Doppler sonography. As an echo-enhancing agent, Sulphur Hexafluoride Microbubbles for Injection （＂SonoVue＂） was administered intravenously in all patients. Sonogram results were reviewed and correlated with angiographic findings. For statistical analysis, the Wilcoxon signed-rank test was *** Angiography identified 20 AVM lesions in the anterior or middle fossa and 3 in the posterior ***-enhanced intraoperative Doppler was somewhat less sensitive for only detecting 21/23 （91.3%） of the AVM lesions. Additionally, contrast-enhanced intraoperative Doppler slightly underestimated AVM size compared with angiographic findings but showed feeding arteries with sufficient acoustic properties. In 15 patients, angiography revealed a coincidental blood supply from another intracranial vessel, which was missed by contrast-enhanced intraoperative Doppler *** In a limited group of patients with AVMs, contrast-enhanced intraoperative Doppler sonography was a less sensitive but useful and simple method for the detection of AVMs in contrast to angiography. No specific untoward effects were attributed to the use of ＂SonoVue＂ as a contrast-enhancing substance.

关键词： arteriovenous malformations intraoperative ultrasonography ultrasound contrast agent

中国高频资产价格对经济增长影响效应及作用路径研究——基于多元混频数据M-MIDAS模型的分析

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内蒙古大学学报（自然科学版） 2017年第4期48卷 386-393页

作者：毛志勇于扬内蒙古财经大学统计与数学学院呼和浩特010070

构建了六种混频数据M-MIDAS模型研究中国高频资产价格对低频经济增长影响效果及预测能力.结果表明:当滞后阶数变动到30阶时,以两参数贝塔(Beta)权重函数构建的MMIDAS模型拟合效果及样本内预测结果最优,Beta-M-MIDAS模型能够提取更多高... 详细信息

构建了六种混频数据M-MIDAS模型研究中国高频资产价格对低频经济增长影响效果及预测能力.结果表明:当滞后阶数变动到30阶时,以两参数贝塔(Beta)权重函数构建的MMIDAS模型拟合效果及样本内预测结果最优,Beta-M-MIDAS模型能够提取更多高频变量股票价格的日数据信息.高频资产价格股票价格、房地产价格对中国经济增长会产生显著正延迟效应,其中房地产价格的影响效应大于股票价格的影响效应.

关键词： M-MIDAS模型资产价格经济增长

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肝再生研究的相关进展和现状

世界华人消化杂志 2016年第30期24卷 4193-4199页

作者：马铎喻智勇昆明医科大学第四附属医院普通外科二科云南省昆明市650021

肝脏具有强大的再生潜能,根据肝脏损伤的程度不同,肝脏表现出不同的再生方式.目前对肝再生的研究取得了一定的成果,对于肝再生的细胞分子生物学机制的研究已经比较深入.最近生物力学因素在肝再生中的作用逐渐受到人们的重视.肝再生过程... 详细信息

肝脏具有强大的再生潜能,根据肝脏损伤的程度不同,肝脏表现出不同的再生方式.目前对肝再生的研究取得了一定的成果,对于肝再生的细胞分子生物学机制的研究已经比较深入.最近生物力学因素在肝再生中的作用逐渐受到人们的重视.肝再生过程除了肝细胞本身的增殖外,还涉及到肝脏干细胞的增殖和分化.但是确切的肝再生机制还未完全清楚.这篇综述将从肝再生的模式、肝再生的细胞分子生物学机制、力学因素对再生的影响以及干细胞在肝再生中的作用这4个方面对肝再生的相关研究进行总结回顾,了解肝再生的相关研究进展,对肝再生认识的不断深入,也为临床上肝脏相关疾病的诊治提供新的思路.

关键词：肝再生部分肝切除术细胞分子生物学干细胞生物力学

非T细胞来源细胞因子在小鼠心脏移植急性排斥反应模型中的表达

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中华医学杂志 2006年第1期86卷 26-30页

作者：梁廷波俞志勇郑树森浙江大学医学院附属第一医院器官移植中心杭州310003

目的研究急性排斥反应性细胞因子在小鼠心脏移植中的表达状况,进一步阐明器官移植急性排斥反应的复杂发生机制。方法采用C57BL/6和Balb/c近交系小鼠,建立小鼠腹腔异位心脏移植模型。随机分为Balb/c-Balb/c同基因对照组(A组)和C57BL/6-Ba... 详细信息

目的研究急性排斥反应性细胞因子在小鼠心脏移植中的表达状况,进一步阐明器官移植急性排斥反应的复杂发生机制。方法采用C57BL/6和Balb/c近交系小鼠,建立小鼠腹腔异位心脏移植模型。随机分为Balb/c-Balb/c同基因对照组(A组)和C57BL/6-Balb/c急性排斥反应组(B组)两组,每组26只。分别于移植术后1、3、5、7 d各处死5只小鼠留取移植心脏标本,采用RT-PCR及W estern印迹方法动态检测组织中IL-15、IL-2、TNF-α及IFN-γ的表达状况,同时行HE染色检测。另外,A、B两组各保留6只小鼠设为亚组,观察移植心脏存活时间。结果A组移植心均获长期存活(>100 d),B组移植心存活时间为8.0 d±0.9 d(t=251.95,P<0.01)。A组各个时间点移植心均未发现排斥现象,B组术后第3天开始出现排斥反应。A组各时间点IL-15 mRNA与TNF-αmRNA呈弱表达,IL-2 mRNA与IFN-γmRNA无表达。B组IL-15 mRNA、TNF-αmRNA、IL-2 mRNA及IFN-γmRNA均明显升高,于第5天达到高峰。A组各时间点可见IL-15、TNF-α、IFN-γ和IL-2蛋白的低水平表达;B组IL-15与IL-2自术后第3天始、TNF-α与IFN-γ自术后第1天始,蛋白表达水平开始升高。结论多种细胞因子,包括非T细胞来源细胞因子,参与了同种异体急性排斥反应的发生。

关键词：移植物排斥心脏移植小鼠细胞因子