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Efficient delivery of clay-based nanovaccines to the mouse spleen promotes potent anti-tumor immunity for both prevention and treatment of lymphoma

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Nano Research 2021年第5期14卷 1326-1334页

作者： Ling-Xiao Zhang Ying-Bo Jia Ya-Ru Huang Hui-Na Liu Xia-Mei Sun Ting Cai Rui-Tian Liu zhi ping xu Hwa Mei Hospital University of Chinese Academy of Sciences(Ningbo No.2 Hospital)Ningbo315010China Ningbo Institute of Life and Health Industry University of Chinese Academy of SciencesNingbo315010China Australian Institute for Bioengineering and Nanotechnology The University of QueenslandSt LuciaQLD4072Australia State Key Laboratory of Biochemical Engineering Institute of Process EngineeringChinese Academy of SciencesBeijing100190China School of Chemical Engineering University of Chinese Academy of SciencesBeijing100049China

Cancer therapeutic nanovaccines are ideal tools to inhibit tumor growth and provide the body with continuous protecting immune ***,the conventional subcutaneous(SC)vaccination normally induces limited anti-tumor immune responses with low therapeutic ***,we devised clay-based nanovaccines and directly delivered them to the spleen via intravenous(IV)injection to induce the stronger anti-tumor immunity with higher efficacy for tumor prevention and *** clay,i.e.,layered double hydroxide(LDH)was prepared as nanoadjuvant with the average size from 77 to 285 nm and co-loaded with the model antigen ovalbumin(OVA)and bioadjuvant CpG to form CpG/OVA-LDH(CO-LDH)*** found that CO-LDH-215(the size of LDH was 215 nm)promoted dendritic cells to present the most antigen,and moreover showed the highest spleen enrichment(~1.67%of CO-LDH-215 enriched in the spleen at 24 h post IV injection).The in vivo immunologic data showed that CO-LDH-215 induced the most potent anti-tumor immune responses and completely prevented the growth of E.G7-OVA tumor in the mouse ***,IV injected CO-LDH-215 nanovaccine more effectively delayed tumor growth than that SC injected,largely due to the direct and quick delivery of more nanovaccines to the *** study demonstrates that the therapeutic efficacy of nanovaccines can be greatly enhanced by targeted delivery of nanovaccines to the spleen via the proper vaccination route.

关键词： cancer immunotherapy nanovaccine layered double hydroxide targeted delivery to the spleen lymphoma

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Higher Education Reform for Computer Major Students in Open and Research Environments

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计算机教育 2012年第10期 57-60页

作者： LI Xin xu Xin-shun JIA zhi-ping MENG Xiang-xu School of Computer Science and Technology Shandong University Jinan 250101 China

This paper analyzes the requirement of professional computer talents in Chinese universities,and introduces the practice in innovative educational methods taken by Shandong University in an open and research *** order to improve educational quality,we have carried out a serial of reforms,including "Four Experiences" aiming at diversifying study environments and fostering their adaptability and extending their *** are encouraged to join "Research Assistant" program and participate in scientific projects to improve their ability in research and *** also conduct "Engineering Practice" to learn latest modeling and programming *** talents characterized of solid foundation,high quality and strong practical ability are shaped through these initiatives.

关键词： Innovation Education Reform Computer Open Research

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BioClay^(TM)prolongs RNA interference-mediated crop protection against Botrytis cinerea

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Journal of Integrative Plant Biology 2022年第11期64卷 2187-2198页

作者： Jonatan Niño-Sánchez Prabhakaran T.Sambasivam Anne Sawyer Rachael Hamby Angela Chen Elizabeth Czislowski Peng Li Narelle Manzie Donald M.Gardiner Rebecca Ford zhi ping xu Neena Mitter Hailing Jin Department of Microbiology&Plant Pathology Center for Plant Cell BiologyInstitute for Integrative Genome BiologyUniversity of CaliforniaRiversideCalifornia92521USA Department of Plant Production and Forest Resources University of ValladolidPalencia 34004Spain iuFOR‐Sustainable Forest Management Research Institute University of Valladolid–INIAPalencia 34004Spain Centre for Planetary Health and Food Security Griffith UniversityNathanQueensland4111Australia School of Chemistry and Molecular Biosciences The University of QueenslandSt LuciaQueensland4072Australia Queensland Alliance for Agriculture and Food Innovation Centre for Horticultural ScienceThe University of QueenslandSt LuciaQueensland4072Australia School of Agriculture and Food Sciences The University of QueenslandSt LuciaQueensland4072Australia Australian Institute for Bioengineering and Nanotechnology The University of QueenslandSt LuciaQueensland4072Australia

One of the most promising tools for the control of fungal plant diseases is spray-induced gene silencing(SIGS).In SIGS,small interfering RNA(siRNA)or double-stranded RNA(dsRNA)targeting essential or virulence-related pathogen genes are exogenously applied to plants and postharvest products to trigger RNA interference(RNAi)of the targeted genes,inhibiting fungal growth and ***,SIGS is limited by the unstable nature of RNA under environmental *** use of layered double hydroxide or clay particles as carriers to deliver biologically active dsRNA,a formulation termed BioClay^(TM),can enhance RNA durability on plants,prolonging its activity against ***,we demonstrate that dsRNA delivered as BioClay can prolong protection against Botrytis cinerea,a major plant fungal pathogen,on tomato leaves and fruit and on mature chickpea *** increased the protection window from 1 to 3 weeks on tomato leaves and from 5 to 10 days on tomato fruits,when compared with naked *** flowering chickpea plants,BioClay provided prolonged protection for up to 4 weeks,covering the critical period of poding,whereas naked dsRNA provided limited *** research represents a major step forward for the adoption of SIGS as an eco-friendly alternative to traditional fungicides.

关键词： Bio Clay double stranded RNA layered double hydroxide RNA interference spray-induced gene silencing

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Nanobody-based bispecific T-cell engager (Nb-BiTE): a new platform for enhanced T-cell immunotherapy

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Signal Transduction and Targeted Therapy 2023年第10期8卷 4444-4447页

作者： Xiao-mei Yang xuan-dong Lin Wei Shi Shen-xia Xie Xia-ning Huang Shi-hua Yin Xiao-bing Jiang Bruce D.Hammock zhi ping xu Xiao-ling Lu Guangxi Key Laboratory of Nanobody Research Guangxi Nanobody Engineering Research CenterCollege of StomatologyHospital of StomatologyLaboratory Animal CenterSchool of Basic Medical SciencePharmaceutical CollegeGuangxi Medical UniversityNanning CityGuangxi 530021P.R.China Department of Neurosurgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan CityHubei 430022P.R.China Department of Entomology and Nematology UCD Comprehensive Cancer CenterUniversity of CaliforniaDavisCA 95616USA Australian Institute for Bioengineering and Nanotechnology The University of QueenslandBrisbaneQLD 4072Australia Institute of Biomedical Health Technology and Engineering and Institute of Systems and Physical Biology Shenzhen Bay LaboratoryShenzhen 518107P.R.China

A novel bispecific T-cell engager(BiTE)has been developed as an efficient immunotherapeutic molecule specifically bringing the T-cell and the tumor target together for enhanced *** general BiTE construct consists of two single-chain variable antibody fragments(scFvs)targeting a tumor-associated antigen(TAA)and a T-cell marker in *** binding of BiTEs to tumor antigens induces immediate T-cells’cytotoxicity against tumor cells without involving any typical costimulatory signals,specific TCR and MHC recognition.

关键词： immunotherapy tumor cytotoxicity

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Relationship　between　loss　of　heterozygosity　of　microsatellite　on　DCC　gene　and　prognosis　of　colorectal　adenocarcinoma

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World Journal of Gastroenterology 1997年第2期3卷 64-65页

作者： ZHAO Po 1, HU Ying Chuan 2, WANG De Wen 3, WANG zhi ping 3, xu Xin Zhao 4, YI ping Yong 2, GAO Ya Bing 3 and YANG Guang Hua 2

AIM To investigate the relationship between the loss of heterozygosity (LOH) of microsatellite on the Deleted in colorectal carcinoma (DCC) gene and the prognosis of colorectal adenocarcinoma. METHODS A retrospective study of 58 cases of colorectal adenocarcinoma with the follow up data and paired control normal mucosal tissues from 1983 to 1985 from files of the Department of Pathology, West China University of Medical Sciences was carried out by PCR microsatellite method. Tumors were graded as well, moderately and poorly differentiated in 16, 35 and 7 cases, and staged as Dukes′ A, B, C in 11, 30 and 17 cases, respectively. RESULTS Heterozygosity of microsatellite was lost on DCC gene in 18 of 58 (31 0%) cases of colorectal adenocarcinoma. The 5 year survival rate between the positive and negative for LOH was 44 4% and 77 5%, respectively, being significantly different ( P 0 05) and Dukes′ stage ( P >0 05) in colorectal adenocarcinoma. CONCLUSION LOH of microsatellite on DCC gene may be one of malignant markers. Combined with the traditional prognostic indices, LOH can predict the prognosis of colorectal adenocarcinoma.

关键词： colorectal neoplasms polymerase chain reaction oncogenes adenocarcinoma heterozygote prognosis

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Cloning and Sequence Analysis of Interleukin-2 Gene in Gaoyou Ducks

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Animal Husbandry and Feed Science 2010年第5期2卷 21-23页

作者： CHEN Cheng SHE Bu-hong REN Chun-zhi TAO Jian-ping xu Jin-jun College of Veterinary Medicine Yangzhou University Yangzhou 225009 China

[ Objective] To clone and analyze the intedeukin-2 （IL.2） gene in Gaoyou ducks. [ Method] With total RNA isolated from peripheral lym- phocytes of Gaoyou ducks as templates, one pair of primers was designed according to the known IL-2 gene sequence in ducks. The IL-2 DNA was amplified by the RT-PCR and inserted into pGEM-T-easy vector. Then the positive recombinant plasmid was sequenced. [ Result] The amplified target fragment was 433 bp with the correct size as theoretically expected. The open reading frame （ORF） was 423 bp encoding a precursor protein with 140 amino acids which consisted of a signal peptide composed of 21 amino acids and a mature peptide composed of 119 amino acids. The pro- tein was approximately 13.66 kDa and contained one N-glycosylation site. The sequence of IL-2 gene of Gaoyou duck had 99.8% nucleotide homol- ogy to that of Anas platyrhynchos duck, Gushi duck and Shaoxing duck, respectively. It also had 99.3% nucleotide homology to that of Guangzhou duck. [ Conclusion] The coding region of IL-2 gene of ducks is highly conserved.

关键词： Gaoyou ducks Interleukin-2 gene Sequence analysis

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Traveling Waves for an Epidemic Model with Vaccination and Nonlocal Diffusion

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Chinese Quarterly Journal of Mathematics 2015年第2期30卷 287-299页

作者： WANG zhi-ping xu Rui ZHANG Shi-hua Department of Basic Courses Shijiazhuang Mechanical Engineering College Shijiazhuang 050003China

An epidemic model with vaccination and nonlocal diffusion is proposed, and the existence of traveling wave solutions of this model is studied. By the cross-iteration scheme companied with a pair of upper and lower solutions and Schauder's fixed point theorem,sufficient conditions are obtained for the existence of a traveling wave solution connecting the disease-free steady state and the endemic steady state.

关键词： vaccination traveling wave solution nonlocal diffusion upper-lower solutions

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聚烯烃老化的时空谱--多因素耦合老化动力学研究

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高分子学报 2021年第11期52卷 1514-1522页

作者：安振华叶焱许治平杨睿清华大学化学工程系北京100084

聚烯烃的自然老化是光照、温度、湿度和氧气多种环境因素综合作用的结果,但长期以来,无法通过实验室加速得到模拟综合条件下的老化结果.本文采用自主设计开发的多因素耦合老化评价系统,可以实现光照、温度、湿度和氧气条件的可控加载,... 详细信息

聚烯烃的自然老化是光照、温度、湿度和氧气多种环境因素综合作用的结果,但长期以来,无法通过实验室加速得到模拟综合条件下的老化结果.本文采用自主设计开发的多因素耦合老化评价系统,可以实现光照、温度、湿度和氧气条件的可控加载,测定高分子材料在不同条件组合下老化时产生的气相降解产物(如CO_(2))的生成速率,以此来评价材料的稳定性.以聚丙烯(PP)和聚乙烯(PE)为典型体系,建立了二者关于温度、光照强度、湿度和氧气浓度的单因子老化速率方程,并基于此建立了PP和PE的多因素耦合老化动力学模型.通过测定不同条件下的老化速率,拟合得到了PP和PE的多因素耦合老化动力学方程,并计算得到了PP和PE的全国“老化速率地图”和逐月“老化速率时间谱”,对PP和PE的应用提供了重要的指导.进行了PP和PE对不同条件因子的敏感性分析.研究发现,PP对光照强度和湿度的敏感性显著高于PE,对氧气浓度的敏感性略高于PE,而对温度的敏感性要低于PE.

关键词：聚丙烯聚乙烯多因素耦合老化动力学

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“One stone, two birds”: Salt template enabling porosity engineering and single metal atom coordinating toward high-performance zinc-ion capacitors

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Journal of Energy Chemistry 2025年第1期100卷 637-645页

作者： Chunliu Zhu Huanyu Liang ping Li Chenglong Qiu Jingyi Wu Jingwei Chen Weiqian Tian Yue Zhu zhi Li Huanlei Wang School of Materials Science and Engineering Ocean University of China Department of Chemical and Materials Engineering University of Alberta

Zinc-ion hybrid capacitors （ZIHCs） have received increasing attention as energy storage devices owing to their low cost,high safety,and environmental ***,their progress has been hampered by low energy and power density,as well as unsatisfactory long-cycle stability,mainly due to the lack of suitable electrode *** this context,we have developed manganese single atoms implanted in nitrogen-doped porous carbon nanosheets （MnSAs/NCNs） using a metal salt template method as cathodes for *** metal salt serves a dual purpose in the synthesis process:It facilitates the uniform dispersion of Mn atoms within the carbon matrix and acts as an activating agent to create the porous *** applied in ZIHCs,the MnSAs/NCNs electrode demonstrates exceptional performance,including a high capacity of 203 m Ah g-1,an energy density of 138 Wh kg-1at 68 W kg-1,and excellent cycle stability with 91%retention over 10,000 *** calculations indicate that the introduced Mn atoms modulate the local charge distribution of carbon materials,thereby improving the electrochemical *** work demonstrates the significant potential of carbon materials with metal atoms in zinc-ion hybrid capacitors,not only in enhancing electrochemical performance but also in providing new insights and methods for developing high-performance energy storage devices.

关键词： Zinc ion hybrid capacitors Cathodes Carbon materials Heteroatoms Single atoms

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Overview of in-situ oxygen production technologies for lunar resources

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 233-255页

作者： Youpeng xu Sheng Pang Liangwei Cong Guoyu Qian Dong Wang Laishi Li Yusheng Wu zhi Wang College of Materials Science and Engineering Shenyang University of TechnologyShenyang 110870China CAS Key Laboratory of Green Process and Engineering National Engineering Research Center for Green Recycling of Strategic Metal ResourcesInstitute of Process EngineeringChinese Academy of SciencesBeijing 100190China Innovation Academy for Green Manufacture Chinese Academy of SciencesBeijing 100190China School of Chemical Engineering University of Chinese Academy of SciencesBeijing 100049China

The rich resources and unique environment of the Moon make it an ideal location for human expansion and the utilization of extraterrestrial ***,crucial for supporting human life on the Moon,can be extracted from lunar regolith,which is highly rich in oxygen and contains polymetallic *** oxygen and metal extraction can be achieved using existing metallurgical ***,the ample reserves of water ice on the Moon offer another means for oxygen *** paper offers a detailed overview of the leading technologies for achieving oxygen production on the Moon,drawing from an analysis of lunar resources and environmental *** delves into the principles,processes,advantages,and drawbacks of water-ice electrolysis,two-step oxygen production from lunar regolith,and one-step oxygen production from lunar *** two-step methods involve hydrogen reduction,carbothermal reduction,and hydrometallurgy,while the one-step methods encompass fluorination/chlorination,high-temperature decomposition,molten salt electrolysis,and molten regolith electrolysis(MOE).Following a thorough comparison of raw materials,equipment,technology,and economic viability,MOE is identified as the most promising approach for future in-situ oxygen production on the *** the corrosion characteristics of molten lunar regolith at high temperatures,along with the Moon's low-gravity environment,the development of inexpensive and stable inert anodes and electrolysis devices that can easily collect oxygen is critical for promoting MOE technology on the *** review significantly contributes to our understanding of in-situ oxygen production technologies on the Moon and supports upcoming lunar exploration initiatives.

关键词： lunar resources in-situ oxygen production space metallurgy molten lunar regolith electrolysis

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