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A bHLH transcription factor,CsSPT,regulates high-temperature resistance in cucumber

Horticultural Plant Journal 2024年第2期10卷 503-514页

作者： Yonggui Liang Chenyu Yang Fangyan Ming Bingwei Yu Zhihua Cheng Yixi Wang zhengkun qiu Xiaolan Zhang Bihao Cao Shuangshuang Yan Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(South China) Ministry of Agriculture and Rural AffairsGuangdong Vegetable Engineering and Technology Research CenterCollege of HorticultureSouth China Agricultural UniversityGuangzhouGuangdong 510642China State Key Laboratories of Agrobiotechnology Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable CropsMOE Joint Laboratory for International Cooperation in Crop Molecular BreedingChina Agricultural UniversityBeijing 100193China

High-temperature stress threatens the growth and yield of crops. Basic helix-loop-helix(bHLH) transcription factors(TFs) have been shown to play important roles in regulating high-temperature resistance in plants. However, the bHLH TFs responsible for high-temperature tolerance in cucumbers have not been identified. We used transcriptome profiling to screen the high temperature-responsive candidate bHLH TFs in cucumber. Here, we found that the expression of 75 CsbHLH genes was altered under high-temperature stress. The expression of the CsSPT gene was induced by high temperatures in TT(Thermotolerant) cucumber plants. However, the Csspt mutant plants obtained by the CRISPR-Cas9 system showed severe thermosensitive symptoms, including wilted leaves with brown margins and reduced root density and cell *** Csspt mutant plants also exhibited elevated H_(2)O_(2) levels and down-regulated photosystem-related genes under normal ***, there were high relative electrolytic leakage(REC), malondialdehyde(MDA), glutathione(GSH), and superoxide radical(O_(2)^(·-)) levels in the Csspt mutant plants, with decreased Proline content after the high-temperature treatment. Transcriptome analysis showed that the photosystem and chloroplast activities in Csspt mutant plants were extremely disrupted by the high-temperature stress compared with wildtype(WT) plants. Moreover, the plant hormone signal transduction, as well as MAPK and calcium signaling pathways were activated in Csspt mutant plants under high-temperature stress. The HSF and HSP family genes shared the same upregulated expression patterns in Csspt and WT plants under high-temperature conditions. However, most bHLH, NAC, and bZIP family genes were significantly down-regulated by heat in Csspt mutant plants. Thus, these results demonstrated that CsSPT regulated the high-temperature response by recruiting photosynthesis components, signaling pathway molecules, and transcription factors. Our results pro

关键词： Cucumber bHLH CsSPT Photosynthesis High temperature

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Analysis of Vitamin P Content and Inheritance Models in Eggplant

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Horticultural Plant Journal 2020年第4期6卷 240-246页

作者： Riyue Dong Bingwei Yu Shuangshuang Yan zhengkun qiu Jianjun Lei Changming Chen Ye Li Bihao Cao College of Horticulture South Agricultural UniversityGuangzhou 510642China Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in South China Ministry of AgricultureChina Harbin Academy of Agricultural Science Harbin 150070China

The eggplant(Solanum melongena L.)is rich in vitamin P(also known as rutin),which is beneficial for *** this study,the vitamin P contents of 53 eggplant lines were measured via UV *** results showed that the vitamin P content differed significantly among the eggplant lines and the actual contents of vitamin P in fresh fruit pulp and fresh peel were 0.18–0.82mg·g^−1 and 0.42–2.32mg·g^−1,***,themixed major gene and polygene inheritance modelmethod was used for the inheritance analysis of the vitamin P content in F2 populations of three hybridization *** analysis demonstrated that the vitamin P content of these eggplant lines was a quantitative *** or two major genes were the contributor,and the genetic effect was an additive-dominance *** high vitamin P content trait was regulated by the main gene and followed an additive *** heritability of the major genes controlling the high vitamin P content was low,while the heritability of the major gene that controls the low vitamin P content trait was *** results provide a theoretical basis for the breeding of eggplants with high vitamin P content.

关键词： eggplant vitamin P hybridization inheritance

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Identification of Regulatory DNA Elements Using Genome-wide Mapping of DNase I Hypersensitive Sites during Tomato Fruit Development

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Molecular Plant 2016年第8期9卷 1168-1182页

作者： zhengkun qiu Ren Li Shuaibin Zhang Ketao Wang Meng Xu Jiayang Li Yongchen Du Hong YU Xia Cui Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture Sino-Dutch Joint Laboratory of Horticultural Genomics Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences Beijing 100081 China State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing) Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China These authors contributed equally to this article.

Development and ripening of tomato fruit are precisely controlled by transcriptional regulation, which de- pends on the orchestrated accessibility of regulatory proteins to promoters and other c/s-regulatory DNA elements. This accessibility and its effect on gene expression play a major role in defining the develop- mental process. To understand the regulatory mechanism and functional elements modulating morpholog- ical and anatomical changes during fruit development, we generated genome-wide high-resolution maps of DNase I hypersensitive sites （DHSs） from the fruit tissues of the tomato cultivar ＂Moneymaker＂ at 20 days post anthesis as well as break stage. By exploring variation of DHSs across fruit development stages, we pinpointed the most likely hypersensitive sites related to development-specific genes. By detecting binding motifs on DHSs of these development-specific genes or genes in the ascorbic acid biosynthetic pathway, we revealed the common regulatory elements contributing to coordinating gene transcription of plant ripening and specialized metabolic pathways. Our results contribute to a better understanding of the regulatory dynamics of genes involved in tomato fruit development and ripening.

关键词： DNase I hypersensitive sites cis-regulatory element gene expression fruit development tomato

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不同逆境胁迫处理对茄子品质指标的影响

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北方园艺 2023年第11期 15-21页

作者：蒋艺邱正坤颜爽爽曹必好华南农业大学园艺学院农业农村部华南地区园艺作物生物学与种质创制重点试验室广东省蔬菜工程技术研究中心广东广州510642 湖南省永州市农业科学研究所湖南永州425000

以“华研5号”茄子为试材,采用基质栽培法,研究了不同逆境(干旱、高温、盐胁迫)处理下茄子的维生素P、可溶性糖、可溶性蛋白质和花青素含量等品质指标变化,以期为生产优质茄子提供参考依据。结果表明:150 mmol·L^(-1)低盐胁迫可以提高... 详细信息

以“华研5号”茄子为试材,采用基质栽培法,研究了不同逆境(干旱、高温、盐胁迫)处理下茄子的维生素P、可溶性糖、可溶性蛋白质和花青素含量等品质指标变化,以期为生产优质茄子提供参考依据。结果表明:150 mmol·L^(-1)低盐胁迫可以提高茄子果实的维生素P含量,降低花青素、可溶性蛋白质和可溶性糖含量;10%PEG 6000轻度干旱胁迫能够提高果实的维生素P、可溶性蛋白质和花青素含量升高,降低可溶性糖含量;40℃高温胁迫可以提高茄子果实的维生素P和可溶性蛋白质含量,降低花青素和可溶性糖含量。

关键词：茄子逆境因子品质指标维生素P

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茄子青枯病抗性相关的E3泛素连接酶基因的筛选及鉴定

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园艺学报 2023年第10期50卷 2271-2287页

作者：王亦栖颜爽爽余炳伟甘雨薇邱正坤朱张生陈长明曹必好华南农业大学园艺学院农业农村部华南地区园艺作物生物学与种质创制重点实验室广东省蔬菜工程技术研究中心广州510642

茄子在生产中易受青枯病侵袭。为挖掘茄子青枯病抗性基因,前期以茄子高抗青枯病自交系和感病自交系为试材进行转录组分析,获得4个与调控青枯病抗性相关的E3泛素连接酶基因,分别命名为SmSP1、SmSPL2、SmDDA1a1和SmDDA1a2。序列分析发现,4... 详细信息

茄子在生产中易受青枯病侵袭。为挖掘茄子青枯病抗性基因,前期以茄子高抗青枯病自交系和感病自交系为试材进行转录组分析,获得4个与调控青枯病抗性相关的E3泛素连接酶基因,分别命名为SmSP1、SmSPL2、SmDDA1a1和SmDDA1a2。序列分析发现,4个E3连接酶的结构域在7个物种中较为保守,SmSP1及SmSPL2属RING型E3连接酶,SmDDA1a1和SmDDA1a2属CRLDDB型E3的底物受体。亚细胞定位表明,除SmDDA1a2定位于细胞核,SmSP1、SmSPL2和SmDDA1a1定位于细胞核及其他细胞部位。4个基因在茄子抗感材料的根、茎及叶中均有表达,叶中最高。青枯菌及外源激素可诱导4个基因的表达,且在抗性材料中的表达量高于感病材料。水杨酸可诱导SmSP1的表达,茉莉酸甲酯可诱导SmSPL2、SmDDA1a1和SmDDA1a2的表达,乙烯利可诱导4个基因的表达。VIGS基因沉默结果显示,在抗性材料中沉默SmSP1和SmDDA1a2后,导致植株青枯病抗性下降,沉默SmSPL2和SmDDA1a1后,对植株青枯菌抗性没有影响。以上结果表明,SmSP1和SmDDA1a2可能参与茄子青枯病抗性的调控。

关键词：茄子青枯病 E3泛素连接酶表达分析功能鉴定

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Identification of regulatory DNA elements using genome-wide mapping of DNase I hypersensitive sites during tomato fruit development

Identification of regulatory DNA elements using genome-wide ...

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2016年全国植物生物学大会

作者： zhengkun qiu Ren Li Shuaibin Zhang Ketao Wang Meng Xu Jiayang Li Yongchen Du Hong Yu Xia Cui Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture Sino-Dutch Joint Laboratory of Horticultural GenomicsInstitute of Vegetables and FlowersChinese Academy of Agricultural Sciences State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing) Institute of Genetics and Developmental BiologyChinese Academy of Sciences

Development and ripening of tomato fruit are precisely controlled by transcriptional regulation,which depends on the orchestrated accessibility of regulatory proteins to promoters and other cis-regulatory DNA elements. This accessibility and its effect on gene expression play a major role in defining the developmental process. To understand the regulatory mechanism and functional elements modulating morphological and anatomical changes during fruit development,we generated genomewide high-resolution maps of DNase I hypersensitive sites(DHSs) from the fruit tissues of the tomato cultivar ?Moneymaker? at 20 days post anthesis as well as break stage. By exploring variation of DHSs across fruit development stages,we pinpointed the most likely hypersensitive sites related to development-specific genes. By detecting binding motifs on DHSs of these development-specific genes or genes in the ascorbic acid biosynthetic pathway,we revealed the common regulatory elements contributing to coordinating gene transcription of plant ripening and specialized metabolic pathways. Our results contribute to a better understanding of the regulatory dynamics of genes involved in tomato fruit development and ripening.

关键词： DNase I hypersensitive sites cis-regulatory element gene expression fruit development tomato

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兼具求解速度与隐私性的复杂供热管网等值简化模型

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电力系统保护与控制 2024年第10期52卷 105-117页

作者：张磊王满康叶婧向紫藤陶千惠杨子龙周正坤邱元城三峡大学电气与新能源学院湖北宜昌443002 中国长江电力股份有限公司湖北宜昌443002

电热能源系统模型维度高将会导致求解难度较大。为此,提出一种兼具求解速度与隐私性的复杂供热管网等值简化模型。首先,基于热网拓扑结构特点,提出可对供热管网进行灵活化简的供热管网拓扑简化方法,并根据简化后的管道结构和参数建立复... 详细信息

电热能源系统模型维度高将会导致求解难度较大。为此,提出一种兼具求解速度与隐私性的复杂供热管网等值简化模型。首先,基于热网拓扑结构特点,提出可对供热管网进行灵活化简的供热管网拓扑简化方法,并根据简化后的管道结构和参数建立复杂供热管网等值简化模型。其次,充分考虑热电联产(combined heat and power,CHP)机组以热定电模式和热网热惯性对旋转备用容量的影响,建立了系统旋转备用模型。基于此,建立了考虑旋转备用约束的电热能源系统调度模型。最后,采用信息间隙决策理论(information gap decision theory, IGDT)处理系统成本与风电不确定度之间的关系。算例结果分析了供热管网拓扑简化方法在不同简化程度下的简化效果,验证了所提模型适用于电热能源系统优化调度,有效地减少优化模型的求解时间。

关键词：电热能源系统拓扑简化旋转备用隐私性风电不确定性

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辣椒红色素及其生物合成的分子机理研究进展

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园艺学报 2023年第3期50卷 669-684页

作者：雷建军朱张生陈长明陈国菊曹必好郑婕吴昊肖艳辉邱正坤颜爽爽韶关学院英东生物与农业学院广东韶关512005 华南农业大学园艺学院农业农村部华南地区园艺作物生物学与种质创制重点实验室广州510642 广东省岭南现代农业实验室广州510642

辣椒红色素包括辣椒红素和辣椒玉红素,是类胡萝卜素生物合成途径的后期产物,是红色辣椒成熟果实品质的重要组成部分,并具有广泛的应用价值。近年来辣椒红色素及其生物合成的分子生物学机理研究取得了重要进展。本文中较系统地介绍了辣... 详细信息

辣椒红色素包括辣椒红素和辣椒玉红素,是类胡萝卜素生物合成途径的后期产物,是红色辣椒成熟果实品质的重要组成部分,并具有广泛的应用价值。近年来辣椒红色素及其生物合成的分子生物学机理研究取得了重要进展。本文中较系统地介绍了辣椒红色素的作用、影响因素、分子标记、基因定位、基因表达、生物合成路径、结构基因的功能鉴定、生物合成和降解、储存调控、利用基因工程技术进行生物生产等方面的研究进展。

关键词：辣椒辣椒红素辣椒玉红素生物合成分子机理保健功能

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Mechanism analysis of solvent selectivity and energy-saving optimization in vapor recompression-assisted extractive distillation for separation of binary azeotrope

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Chinese Journal of Chemical Engineering 2022年第6期35卷 271-279页

作者： Xiaomin qiu Yuanyuan Shen zhengkun Hou Qi Wang Zhaoyou Zhu Yinglong Wang Jingwei Yang Jun Gao College of Chemical Engineering Qingdao University of Science and TechnologyQingdao 266042China College of Chemical and Environmental Engineering Shandong University of Science and TechnologyQingdao 266590China

Octane and p-xylene are common components in crude gasoline,so their separation process is very important in petroleum *** azeotrope and near azeotrope are often separated by extractive distillation in industry,which can realize the recovery and utilization of *** this work,the vapor–liquid equilibrium experiment was used to obtain the vapor–liquid equilibrium properties of the difficult separation system,and on this basis,the solvent extraction mechanism was *** mechanism of solvent separation plays a guiding role in selecting suitable solvents for industrial *** interaction energy,bond length and charge density distribution of p-xylene with solvent are calculated by quantum chemistry *** quantum chemistry calculation results and experiment results showed that N-formylmorpholine is the best solvent among the alternative solvents in the *** work provides an effective and complete solvent screening process from phase equilibrium experiments to quantum chemical *** extractive distillation simulation process with N-formylmorpholine as solvent is designed to separate octane and *** addition,the feasibility and effectiveness of the intensified vapor recompression assisted extraction distillation are also *** the extractive distillation process,the vapor recompression-assisted extraction distillation process is globally *** with basic process,the total annual cost can be reduced by 43.2%.This study provides theoretical guidance for extractive distillation separation technology and solvent selection.

关键词： Quantum chemical calculation Vapor liquid equilibria Distillation Energy saving Separation

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Energy,exergy,economic and environmental comprehensive analysis and multi-objective optimization of a sustainable zero liquid discharge integrated process for fixed-bed coal gasification wastewater

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Chinese Journal of Chemical Engineering 2023年第6期58卷 341-354页

作者： Yanli Zhang zhengkun Hou Dong Yao Xiaomin qiu Hongru Zhang Peizhe Cui Yinglong Wang Jun Gao Zhaoyou Zhu Limei Zhong College of Chemical Engineering Qingdao University of Science and TechnologyQingdao 266042China College of Chemical and Environmental Engineering Shandong University of Science and TechnologyQingdao 266590China

For a long time,China's regional water resource imbalance has restricted the development of coal chemical industry,and it is imperative to achieve zero liquid discharge(ZLD).Therefore,the game relationship between technical indicators,costs and emissions in ZLD process of fixed-bed coal gasification wastewater treatment process should be explored in *** to the accurate model,the simulation for ZLD of fixed-bed coal gasification wastewater treatment process is established,and this process is assessed from the perspective of thermodynamics,economy,and *** total energy consumption of ZLD process before optimization is 4.032×10^(8)*** results of exergy analysis show exergy destruction of ZLD process is 94.55%.For economic and environmental results,the total annual cost is 1.892×10^(7)USD·a^(-1)and the total environmental impact is 4.782×10^(-8).The total energy consumption of the optimal six-step ZLD process based on multi-objective optimization is 4.028×10^(8)*** CO_(2)content in the treated wastewater is 0.1%.This study will have an important role in promoting the establishment of the ZLD process for coal chemistry industry.

关键词： Waste water Case study Energy Exergy Economy Environment

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