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Epigenetic regulation of Wnt signaling pathway gene SRY-related HMG-box 17 in papillary thyroid carcinoma

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Chinese Medical Journal 2012年第19期125卷 3526-3531页

作者： LI Jing-yi HAN Chao zheng Li-li GUO Ming-zhou Center for Mammoplasty and Breast Reconstruction Plastic Surgery Hospital Peking Union Medical College Chinese Academy of Medical Sciences Beijing 100144 China Department of Endocrinology and Metabolism the First Affiliated Hospital of Zhengzhou University Zbengzhou Henan 450052 China Department of Gastroenterology & Hepatology Chinese People's Liberation Army General Hospital Beijing 100853 China

Background SRY-related HMG-box 17 （SOX17） encodes a member of the SOX （SRY-related HMG-box） family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. Recently, it was considered as a tumor suppressor gene to inhibit canonical Wnt/β-catenin signaling pathway in several malignancies. However, the function of SOX17 in thyroid cancer was unknown. Therefore, we investigated the epigenetic changes and the function of SOX17 in thyroid cancer. Methods The methylation status of the promoter region of SOX17 was detected using methylation-specific PCR in 63 papillary thyroid carcinoma （PTC） tissue, 10 normal thyroid tissue, and two thyroid cancer cell lines. Semi-quantitative RT-PCR was used to assess mRNA expression of SOX17 before and after 5-aza-2＇-deoxycytidine treatment in thyroid cancer cell lines. Expression of SOX17 and β-catenin were detected by immunohistochemistry in PTC and adjacent tissue. Luciferase reporter assay, colony formation, transfection, and Western blotting were employed to analyze the effect of SOX17 on thyroid cancer cell proliferation and the function of SOX17 in the Wnt signal pathway. Results Loss of SOX17 expression was correlated to the promoter region hypermethylation in thyroid cancer cell lines. Re-expression of SOX17 was found in TPC-1 cell line after 5-aza-2＇-deoxycytidine treatment. In primary thyroid cancer, 60.3% （38/63） were methylated and 39.7% （25/63） unmethylated. But no methylation was found in noncancerous thyroid tissues. Methylation of SOX17 was associated reversely with β-catenin expression in the cytoplasm or nucleus significantly in the PTC （P 〈0.05）. Colony formation was inhibited by re-expression of SOX17 in TPC-1 cells. SOX17 suppressed the Wnt signaling pathway and the HMG domain was essential for this effect. Conclusions SOX17 was frequently methylated in human PTC. Loss of SOX17 expression was induced by promoter region hypermethylation. SOX17 inhibited

关键词： DNA methylation SRY-related HMG-box 17 papillary thyroid carcinoma Wnt signaling pathway tumor suppressor gene

Solubilization of Rock Phosphate in Liquid Culture by Fungal Isolates from Rhizosphere Soil

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Pedosphere 2005年第4期15卷 532-538页

作者： WANG Guang-hua zhou De-Rui YANG Qian JIN Jian LIU Xiao-Bing Department of Applied Chemistry Harbin Institute of Technology Harbin 150001 （China） Northeast Institute of Geography and Agricultural Ecology Chinese Academy of Sciences Harbin 150050 （China）

Rock phosphate （RP） is a low efficiency P fertilizer that is directly applied to the soil and can be solubilized by phosphate-solubilizing microorganisms （PSMs） in fermentation or soil conditions. This study investigated dynamic solubilization of 2 concentrations of rock phosphate in a liquid culture with different dosages of glucose by two fungal isolates,Aspergillus niger P39 and Penicillium oxalicum P66, from soybean and wheat rhizosphere soil. Although during the 20 day culture period A. niger P39 had a stronger ability to acidify the culture media than P. ozalicum P66, soluble P concentrations at glucose dosages of 30 and 50 g L^-1 with RP of 15 g L^-1 in the culture solution were much higher by P. oxalicum P66. The greater effectiveness of P. oxalicum P66 compared to A. niger P39 in the solubilization of RP was strongly associated with the production of organic acids. This study suggested that for RP solubilization the type rather than the concentration of PSM-produced organic acids was more important.

关键词： Aspergillus niger organic acids Penicillium oxalicum phosphate solubilization rock phosphate

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基于免疫遗传算法的车间天车调度仿真模型

系统工程理论与实践 2013年第1期33卷 223-229页

作者：郑忠周超陈开重庆大学材料科学与工程学院重庆400050

研究了多机多任务的车间天车调度问题,提出了一种结合免疫遗传算法的仿真模型解决方案.该方案建立了能反映天车实际工作环境运行特征的仿真模型,根据天车作业跨的工位与天车位置关系进行模型空间抽象,用可变的天车任务优先级来解决天车... 详细信息

研究了多机多任务的车间天车调度问题,提出了一种结合免疫遗传算法的仿真模型解决方案.该方案建立了能反映天车实际工作环境运行特征的仿真模型,根据天车作业跨的工位与天车位置关系进行模型空间抽象,用可变的天车任务优先级来解决天车运行过程中空间约束导致的多机多任务冲突;仿真模型用于评估各种调度方案,免疫遗传算法则使调度方案在不断的迭代中持续优化.以某钢厂一主作业跨的天车调运任务问题制定天车调度方案进行模型检验,对求解的可行天车任务分配方案,进行比较分析,说明了模型方法的有效性与工程应用的可行性.

关键词：多机多任务天车调度仿真模型免疫遗传算法

Enhancement of beneficial microbiomes in plant-soil continuums through organic fertilization:Insights into the composition and multifunctionality

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Soil Ecology Letters 2024年第3期6卷 109-120页

作者： Wen-Jing Li Xin-Yuan zhou Xin-Li An Li-Juan Li Chen-Shuo Lin hua Li Hong-Zhe Li Key Lab of Urban Environment and Health Institute of Urban EnvironmentChinese Academy of SciencesXiamen 361021China University of Chinese Academy of Sciences Beijing 100049China State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Institute of EnvironmentResourceSoil and FertilizersZhejiang Academy of Agricultural SciencesHangzhou 310021China

Soil microbiomes play a crucial role in maintaining ecological functions and are of great importance for soil *** of them could bring benefits to plants for growth *** numerous studies have focused on specific beneficial bacteria and their interactions with soils and plants,we still lack a comprehensive understanding of beneficial communities in plant–soil continuums and their responses to agricultural *** address this gap,we carried out a microcosm experiment using 16S rRNA amplicon sequencing to explore the effects of organic fertilization on beneficial communities in plant–soil continuums and assess their potential *** findings reveal that organic fertilization had a positive impact on the beneficial functionality of bacterial communities in plant–soil *** improvement was primarily attributed to the optimized soil physicochemical conditions resulting from organic ***,organic fertilization increased the complexity of bacterial co-occurrence networks in both soils and the *** taxa in the endosphere undergone a shift of functions toward pathogen suppression as the result of organic ***,this study revealed that plants exhibited a preference for internalizing beneficial bacteria over other type of *** also provided new insights for evaluating the multifunctionality of microbiomes,and found that the functionality of beneficial communities in plant–soil continuums is enhanced by organic *** these findings suggested that organic fertilization can be an effective strategy for maintaining plant and soil health.

关键词： plant–soil continuums beneficial bacteria agricultural activities multifunctionality endosphere

Genesis of the Gold Deposit in the Indus-Yarlung Tsangpo Suture Zone,Southern Tibet:Evidence from Geological and Geochemical Data

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Acta Geologica Sinica(English Edition) 2017年第3期91卷 947-970页

作者： ZHANG Xiong DENG Xueguo YANG Zhusen HOU Zengqian zheng Yuanchuan LIU Yingchao ZHAO Xiaoyan XU Bo PEI Yingru zhou Jinsheng ZHAO Miao YUAN Jianfei School of Earth sciences and Resources China University of GeosciencesBeijing MLR Key Laboratory of Metallogeny and Mineral Assessment Institute of Mineral ResourcesChinese Academy of Geological Sciences Institute of Metallurgical Geology and Exploration Sichuan Institute of Geology Chinese Academy of Geological Sciences ARC Centre of Excellence for Core to Crust Fluid System(CCFS) and GEMOC Department Of Earth and Planetary SciencesMacquarie University

The Nianzha gold deposit, located in the central section of the Indus-Yarlung Tsangpo suture （IYS） zone in southern Tibet, is a large gold deposit （Au reserves of 25 tons with average grade of 3.08 g/t） controlled by a E-W striking fault that developed during the main stage of Indo-Asian collision （-65-41 Ma）. The main orebody is 1760 m long and 5.15 m thick, and occurs in a fracture zone bordered by Cretaceous diorite in the hanging wall to the north and the Renbu tectonic melange in the footwall to the south. High-grade mineralization occurs in a fracture zone between diorite and ultramafic rock in the Renbu tectonic melange. The wall-rock alteration is characterized by silicification in the fracture zone, serpentinization and the formation of talc and magnesite in the uitramafic unit, and chloritization and the formation of epidote and calcite in diorite. Quartz veins associated with Au mineralization can be divided into three stages. Fluid inclusion data indicate that the deposit formed from H20-NaCl-organic gas fluids that homogenize at temperatures of 203℃-347℃ and have salinities of 0.35wt%-17.17wt% NaCI equivalent. The quartz veins yield δ18Ofluid values of 0.15‰-10.45‰, low δDv-SMow values （-173%o to -96%o）, and the δ13C values of-17.6‰ to -4.7‰, indicating the ore-forming fluids were a mix of metamorphic and sedimentary orogenic fluids with the addition of some meteoric and mantle-derived fluids. The pyrite within the diorite has δ34SV-CDT values of -2.9‰-1.9‰（average -1.1‰）, 206pb/204pb values of 18.47- 18.64, 207pb/204pb values of 15.64-15.74, and 208pb/204pb values of 38.71-39.27, all of which are indicative of the derivation of S and other ore-forming elements from deep in the mantle. The presence of the Nianzha, Bangbu, and Mayum gold deposits within the IYS zone indicates that this area is highly prospective for large orogenic gold deposits. We identified three types of mineralization within the IYS, namely Bangbu-type accretionary, Mayum-type micro

关键词： geology gold mineralization Nianzha Deposit Indus-Yarlung Tsangpo suture zone Tibet

Attenuated plasmodium sporozoite expressing MAGE-A3 induces antigen-specific CD8+ T cell response against lung cancer in mice

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Cancer Biology & Medicine 2019年第2期16卷 288-298页

作者： Dong zhou Hong zheng Quanxing Liu Xiao Lu Xufeng Deng Li Jiang Bing Hou Yong Fu Feng Zhu Yan Ding Wenyue Xu Jigang Dai Department of Thoracic Surgery Xinqiao Hospital Third Military Medical University Chongqing 400037 China Department of Cardiothoracic Surgery The First People's Hospital of Zunyi Zunyi 563000 China Department of Pathogenic Biology Third Military Medical University Chongqing 400037 China

Objective: Cancer vaccines that rely on tumor antigen-specific CD8^+ T cell responses, are promising anti-cancer adjuvant immunotherapies. This study investigated whether genetically attenuated Plasmodium sporozoite(GAS) could be used as a novel vector to induce antigen-specific CD8^+ T cell responses against lung ***: We constructed GAS/MAGE-A3, a recombinant GAS engineered to express the lung cancer-specific antigen, melanomaassociated antigen 3(MAGE-A3), and assessed its therapeutic effects against lung ***: Robust parasite-specific CD8αlow CD11ahigh and CD49dhigh CD11ahigh CD4^+ T cell responses as well as a MAGE-A3-specific CD8^+ T cell response were induced in GAS/MAGE-A3-immunized mice. Adoptive transfer of GAS/MAGE-A3-induced CD8^+ T cells from HLA-A2 transgenic mice into lung cancer-bearing nude mice inhibited tumor growth and prolonged ***: These findings demonstrate that GAS/MAGE-A3 induces a strong MAGE-A3-specific CD8^+ T cell response against lung cancer in vivo, and indicate that GAS is a novel and efficacious antigen delivery vector for antitumor immunotherapy.

关键词： Vaccine lung cancer genetically attenuated sporozoites MAGE-A3 malaria

Depressive Symptoms and Sleep Duration as Risk Factors for Breast Cancer—China,2020

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China CDC weekly 2023年第15期5卷 333-339页

作者： Liyuan Liu Heling Bao Fei Wang Lixiang Yu Shu Cong Fei zhou Yujuan Xiang Shuya huang Chao zheng Liwen Fang Linhong Wang Zhigang Yu Department of Breast Surgery The Second Hospital of Shandong UniversityJinan CityShandong ProvinceChina Institute of Translational Medicine of Breast Disease Prevention and Treatment Shandong UniversityJinan CityShandong ProvinceChina Institute of Medical Information Chinese Academy of Medical Sciences&Peking Union Medical CollegeBeijingChina National Center for Chronic and Noncommunicable Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijingChina

Summary What is already known about this topic?Psychological and lifestyle factors are known to potentially play a significant role in the development of breast ***,current evidence-based studies present controversial... 详细信息

关键词： breast Breast Cancer

Association between body mass index and the risk of bleeding in elderly patients with non-valvular atrial fibrillation taking dabigatran:a cohort study

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Journal of Geriatric Cardiology 2020年第4期17卷 193-201页

作者： Ming-Hui LI Li-hua HU Yu-Rong XIONG Yu YU Wei zhou Tao WANG Ling-Juan ZHU Xi LIU Hui-Hui BAO Xiao-Shu CHENG Department of Cardiovascular Medicine the Second Affiliated Hospital of Nanchang UniversityJiangxiChina Center for Prevention and Treatment of Cardiovascular Diseases the Second Affiliated Hospital of Nanchang UniversityJiangxiChina Center of Cardiovascular Inner Mongolia People’s HospitalHohhotInner MongoliaChina

Background Uncertainty remains regarding the association between body mass index(BMI)and the risk of bleeding in patients with non-valvular atrial fibrillation(NVAF).We aimed to investigate the association between BMI and the risk of bleeding in elderly NVAF patients taking *** A total of 509 elderly NVAF patients,who were being treated at twelve centers in China from February 2015 to December 2017 and taking dabigatran,were *** exposure and outcome variables were BMI at baseline and bleeding events within the subsequent six months,*** proportional hazards regression analysis was used to evaluate the association between BMI and the risk of ***,the Cox proportional hazards regression with cubic spline functions and smooth curve fitting was *** During the six-month follow-up,50 participants experienced *** 1 kg/m^2 increase in BMI was associated with a 12%increased risk of bleeding(P=0.021).Compared to those with BMI values in Tertile 1(25.3 kg/m^2)were 2.71(95%CI:1.02–7.17)and 3.5(95%CI:1.21–8.70),*** Ptrend-value was significant in all *** adjusted smooth curve showed a linear association between BMI and *** of the stratified variables showed significant effect modification on the association between BMI and bleeding(Pinteraction>0.05).Conclusions BMI was significantly and positively associated with the risk of bleeding in elderly NVAF patients treated with dabigatran.

关键词： Atrial fibrillation Bleeding Body mass index Dabigatran

High-Efficiency InGaN/GaN Nanorod Arrays by Temperature Dependent Photoluminescence

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Chinese Physics Letters 2013年第7期30卷 247-250页

作者： WANG Wen-Jie CHEN Peng YU Zhi-Guo LIU Bin XIE Zi-Li XIU Xiang-Qian WU Zhen-Long XU Feng XU zhou hua Xue-Mei ZHAO Hong HAN Ping SHI Yi ZHANG Rong zheng You-Dou Jiangsu Provincial Key Laboratory of Advanced Photonic and Electronic Materials and School of Electronic Science and Engineering Nanjing UniversityNanjing 210093 Nanjing University Institute of Optoelectronics at Yangzhou Yangzhou 225009

We report on the photoluminescent characteristics of InGaN/GaN multiple quantum well(MQW)nanorod arrays with high internal quantum *** InGaN/GaN MQWs are grown by metalorganic chemical vapor deposition on c-plane sapphire substrates,and then the MQW nanorod arrays are fabricated by using inductively coupled plasma etching with self-assembled Ni nanoparticle mask with low-damage etching *** typical diameter of the nanorods is from 200 nm to 300 nm and the length is around 800 nm,which almost is dislocation *** room temperature,an enhancement of 3.1 times in total integrated photoluminescence intensity is achieved from the MQW nanorod arrays,in comparison to that of the as-grown MQW *** on the temperature-dependent photoluminescence measurements,the internal quantum efficiency of the nanorod structure is 59.2%,i.e.,1.75 times of as-grown MQW structure(33.8%).Therefore,the nanorod structure with a significant reduction of defects can be a very promising candidate for highly efficient light emitting devices.

关键词： InGaN/GaN technique. Efficiency