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滚子链简易拆装工具

起重运输机械 2011年第3期 90-90页

作者：韩强郑迎梅太原锅炉集团太原030021 太原重工齿轮传动设计研究所太原030024

链传动的优点是：没有滑动；工况相同时，传动尺寸比较紧凑；不需要很大的张紧力，作用在轴上的载荷较小；效率较高，可达98％；能在较恶劣的环境下使用；可用于轴距较大的场合。

关键词：拆装工具滚子链链传动尺寸比张紧力轴距

Recombinant human PDCD5 protein enhances chemosensitivities of hematologic malignancies

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Chinese Science Bulletin 2009年第21期54卷 3981-3989页

作者： WANG YanFang SHI Lin SONG QuanSheng ZHANG yingmei LOU YaXin zheng Yi MA DaLong WANG Ying KE XiaoYan Department of Hematology Peking University Third Hospital Beijing 100191 China Peking University Center for Human Disease Genomics Beijing 100191 China Department of Medical Immunology School of Basic Medical Sciences Peking University Health Science Center Beijing 100191 China Peking University Medical and Health Analysis Center Peking University Health Science Center Beijing 100191 China

PDCD5 is a novel apoptosis-related gene. Overexpression of PDCD5 facilitates apoptosis in various tumor cells. Here, we investigated the roles of recombinant human PDCD5 (rhPDCD5) protein in chemosensitivities of hematologic malignancies. The rhPDCD5 increased the in vitro cytotoxicity of daunorubicin (DNR), adriamycin (ADM) and As2O3 in three hematologic tumor cell lines. In vivo studies showed that DNR combined with rhPDCD5 significantly suppressed tumor growth of U937 xenograft nude mice compared with DNR alone. In conclusion, rhPDCD5 combined with the chemotherapeutic drug has greater efficacy than chemotherapy alone, and rhPDCD5 is a very promising chemosensitizer.

关键词： PDCD5 白蛋白血液病恶性重组肿瘤细胞凋亡柔红霉素凋亡相关基因

Distinct regulatory mechanism of immunoglobulin gene transcription in epithelial cancer cells

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Cellular & Molecular Immunology 2010年第4期7卷 279-287页

作者： Xiaohui Zhu Lina Wu Li Zhang Peng Hao Shuai Zhang Jing Huang Jie zheng Yinan Liu Wenjun Li yingmei Zhang Chunyan Zhou Youhui Zhang C Cameron Yin Xiaoyan Qiu Peking University Center for Human Disease Genomics BeijingChina Department of Biochemistry School of Basic Medical SciencePeking UniversityBeijingChina Department of Immunology Cancer InstituteChinese Academy of Medical Science and Peking Union Medical CollegeBeijingChina Department of Hematopathology University of Texas M.D.Anderson Cancer CenterHoustonTXUSA These authors contributed equally to this work

The restriction of immunoglobulin(Ig)expression to B lymphocytes is well ***,several reports have confirmed that the Ig gene can be expressed in many non-B cancer cells and/or some normal *** aim is to determine whether the Ig gene promoter can be activated in non-B cancer cells and to identify the regulatory mechanism for Ig gene *** results show that the Ig promoter of VH4-59 was activated in several non-B cancer cell ***,two novel positive regulatory elements,an enhancer-like element at 2800 to 2610 bp and a copromoter-like element at 2610 to 2300 bp,were identified in two epithelial cancer cell lines,HeLa S3 and *** octamer element(59-ATGCAAAT-39)located in the Ig promoter,a crucial element for B-cell-derived Ig gene transcription,was also very important for non-B-cell-derived Ig gene *** importantly,we confirmed that octamer-related protein-1(Oct-1),but not Oct-2,was a crucial transcriptional factor for Ig gene transcription due to its ability to bind to the octamer element of the Ig promoter in epithelial cancer *** results suggested the presence of a distinct regulatory mechanism for Ig gene expression in non-B cancer cells.

关键词： Ig gene transcription Oct-1 promoter transcription regulation VH4-59

Click CAR-T cell engineering for robustly boosting cell immunotherapy in blood and subcutaneous xenograft tumor

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Bioactive Materials 2021年第4期6卷 951-962页

作者： Hong Pan Wenjun Li Ze Chen yingmei Luo Wei He Mengmeng Wang Xiaofan Tang Huamei He Lanlan Liu Mingbin zheng Xin Jiang Ting Yin Ruijing Liang Yifan Ma Lintao Cai Guangdong Key Laboratory of Nanomedicine CAS-HK Joint Lab of BiomaterialsCAS Key Lab for Health InformaticsShenzhen Engineering Laboratory of Nanomedicine and NanoformulationsShenzhen Institutes of Advanced Technology(SIAT)Chinese Academy of SciencesShenzhen518055PR China HRYZ Biotech Co. Shenzhen518057PR China

The adoptive transfer of chimeric antigen receptor-T(CAR-T)cells has shown remarkable clinical responses in hematologic ***,unsatisfactory curative results and side effects for tumor treatment are still unsolved *** we develop a click CAR-T cell engineering strategy via cell glycometabolic labeling for robustly boosting their antitumor effects and safety in ***,paired chemical groups(N3/BCN)are separately incorporated into CAR-T cell and tumor via nondestructive intrinsic glycometabolism of exogenous Ac4GalNAz and Ac4ManNBCN,serving as an artificial *** groups anchored on cell surface strengthen the interaction of CAR-T cell and tumor via bioorthogonal click chemistry,further enhancing specific recognition,migration and selective antitumor effects of CAR-T *** vivo,click CAR-T cell completely removes lymphoma cells and minimizes off-target toxicity via selective and efficient bioorthogonal targeting in blood ***,compared to unlabeled cells,artificial bioorthogonal targeting significantly promotes the accumulation,deep penetration and homing of CAR-T cells into tumor tissues,ultimately improving its curative effect for solid *** CAR-T cell engineering robustly boosts selective recognition and antitumor capabilities of CAR T cells in vitro and in vivo,thereby holding a great potential for effective clinical cell immunotherapy with avoiding adverse events in patients.

关键词： CAR-T cell Bioorthogonal click chemistry Glycometabolic labeling Off-target effects Cell immunotherapy

Modification of alternative splicing in bovine somatic cell nuclear transfer embryos using engineered CRISPR-Cas13d

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Science China(Life Sciences) 2022年第11期65卷 2257-2268页

作者： Rui Cheng Xiaoman zheng yingmei Wang Xing Ma Xin Liu Wenjun Xu Mengyun Wang Yuanpeng Gao Xupeng Xing Chuan Zhou Hongzheng Sun Zekun Guo Fusheng Quan Jun Liu Song Hua Yongsheng Wang Yong Zhang Xu Liu College of Veterinary Medicine Northwest A&F UniversityKey Laboratory of Animal Biotechnology of the Ministry of AgricultureYangling 712100China

Animal cloning can be achieved by somatic cell nuclear transfer(SCNT),but the resulting live birth rate is relatively *** previously improved the efficiency of bovine SCNT by exogenous melatonin treatment or by overexpression of lysine-specific demethylase 4D(KDM4D)and 4E(KDM4E).In this study,we revealed abundant alternative splicing(AS)transitions during fertilization and embryonic genome activation,and demonstrated abnormal AS in bovine SCNT embryos compared with in vitro fertilized *** used the CRISPR-Cas13d RNA-targeting system to target cis-elements of ABI2 and ZNF106 pre-m RNA to modify AS,thus reducing the ratio of abnormal-isoform SCNT embryos by nearly 50%and achieving a high survival rate(11%–19%).These results indicate that this system may provide an efficient method for bovine cloning,while also paving the way for further improvements in the efficiency of SCNT.

关键词： animal cloning alternative splicing CRISPR-Cas13d bovine

Patient-specific Seizure Prediction with Scalp EEG Using Convolutional Neural Network and Extreme Learning Machine

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Patient-specific Seizure Prediction with Scalp EEG Using Con...

Automatic Seizure Prediction from Scalp EEG with Optimal Feature and Minimum Channels

第三十九届中国控制会议

作者： yingmei Qin Hailing zheng Wei Chen Qing Qin Chunxiao Han Yanqiu Che Tianjin Key Laboratory of Information Sensing & Intelligent Control School of Automation and Electrical EngineeringTianjin University of Technology and Education IEEE Department of Neurosurgery Penn State College of Medicine

Seizure prediction can allow timely preventive measures for patients with epilepsy. In this study, we propose a hybrid model consisting of convolutional neural networks（CNNs） and an extreme learning machine（ELM） to predict seizures using scalp EEG. We first covert the EEG time series on 30-s windows into 2 D spectrograms using the short-time Fourier *** we apply CNNs to these images to extract features automatically. Finally, we use the ELM to classify preictal and interictal segments. The proposed method achieves sensitivity of 95.85% and a false prediction rate of 0.045/h on the Boston Children’s Hospital-MIT scalp EEG dataset.

关键词： Seizure Prediction EEG Convolutional Neural Networks Extreme Learning Machine

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Automatic Seizure Prediction from Scalp EEG with Optimal Fea...

High-dimensional immune profiling by mass cytometry revealed immunosuppression and dysfunction of immunity in COVID-19 patients

第32届中国控制与决策会议

作者： Hailing zheng yingmei Qin Qing Qin Wei Chen Jingyu Zhang Jian Dong Tianjin Key Laboratory of Information Sensing ＆ Intelligent Control School of Automation and Electrical EngineeringTianjin University of Technology and Education

The aim of this study is to develop an efficient,reliable and automatic epileptic seizure prediction system using scalp EEG measurements with optimal feature and minimum *** data in interictal and preictal periods from the CHB-MIT dataset are used for seizure ***,the original signals are decomposed into several frequency bands using a digital wavelet transform（DWT）.Then,features including standard deviation（S）,log of amplitude（L）,quartile（Q） and coefficient of variation（CV） are ***,different combinations of feature vectors are fed into classifiers（support vector machine（SVM） and extreme learning machine（ELM））to classify the above two states（preictal and interictal states）.Performance analysis shows that the optimal feature is CV,the optimal sub-band is 16-31 Hz and the optimal EEG channel can be chosen as FP1-F7,T7-P7,FP1-F3,P3-O1 or *** comparing the classification results,ELM provides a more robust and higher overall accuracy than SVM,and the best average accuracy of both ELM and SVM can reach as high as 100%.

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Cellular & Molecular Immunology 2020年第6期17卷 650-652页

作者： Wenjing Wang Bin Su Lijun Pang Luxin Qiao yingmei Feng Yabo Ouyang Xianghua Guo Hongbo Shi Feili Wei Xiaogang Su Jiming Yin Ronghua Jin Dexi Chen Beijing Institute of Hepatology Beijing Youan HospitalCapital Medical University100069 BeijingChina Beijing Precision Medicine and Transformation Engineering Technology Research Center of Hepatitis and Liver Cancer 100069 BeijingChina Center for Infectious Diseases Beijing Youan HospitalCapital Medical University100069 BeijingChina

The outbreak of coronavirus disease 2019(COVID-19)caused by the new virus SARS-CoV-2 has been announced as a public health emergency of international concern.1–3 The clinical features of patients with COVID-19 range from common fever and cough to other rare symptoms,such as diarrhea and *** disease can progress quickly,and 2–3%of patients die within a short time,which is generally due to multiple organ failure.4–7 Clinically,COVID-19 patients are classified into mild,moderate,severe,and critical cases.5–7 The immune response against SARS-CoV-2 is probably linked to the severity of ***,zheng et al.8 showed that elevated levels of T-cell exhaustion and reduced functional diversity of T cells in peripheral blood may predict severe progression in COVID-19 patients;however,a more comprehensive understanding of the pathology of SARS-CoV-2 infection remains to be ***,we profiled immune cellular components using mass cytometry(CyTOF)to analyze the peripheral blood mononuclear cells(PBMCs)from patients with differences in disease progression by comparing with the PBMCs from healthy donors(HDs).

关键词： patients immunity elevated

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昆明市县区收入差距的时空演进及其成因分析

地域研究与开发 2011年第1期30卷 74-78页

作者：李正武友德舒劲吴映梅云南师范大学旅游与地理科学学院昆明650092

以昆明市各县区为研究单位,对收入差距问题作了实证分析。采用统计法和建立绝对β-收敛模型测度了县区收入差距,描述其时序过程和空间演替特征;利用Theil指标法揭示区域差距的构成与来源,并运用回归法探讨其影响因素。结论证实,昆明市... 详细信息

以昆明市各县区为研究单位,对收入差距问题作了实证分析。采用统计法和建立绝对β-收敛模型测度了县区收入差距,描述其时序过程和空间演替特征;利用Theil指标法揭示区域差距的构成与来源,并运用回归法探讨其影响因素。结论证实,昆明市区域差异演进并没有偏离收敛性假说:县区收入差距以2003年为拐点呈阶段性律动,总体差距趋小而梯度间差距和城乡间差距深化,社会消费、储蓄、财政支出是其影响因素,而普遍认为有促进作用的市场因素在概念模型分析中却不显著。为此,发挥市场力量势在必行。

关键词：县区收入差距时空演进收敛性假说差异分解影响因素昆明市