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Cold Simulation of Oxygen Transfer Rate in BOF

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Journal of Iron and Steel Research International 2010年第9期17卷 7-13页

作者： WU Wei YANG Li-bin zheng cong-jie LIU Liu Central Iron and Steel Research Institute Beijing 100081 China

By measuring the mass transfer coefficient of benzoic acid between water and oil, the oxygen transfer rate in BOF bath was researched, and the influence of top and bottom blowing gas flow rate on the mass transfer between metal and slag was discussed. The results show that with increasing the bottom blowing gas flow rate under the conditions of fixed top blowing, the mass transfer rate evidently increases, and the influence of fixed top blowing on the mass transfer is 10% of bottom blowing. The stirring intensity was determined as a function of top blowing gas flow rate, bottom blowing gas flow rate, and lance height. The equation of the mass transfer coefficient between metal and slag was established. The relationship between the emulsification ratio of oil to water and the bottom blowing gas flow rate under the conditions of top and bottom blowing was obtained. The result shows that with the increase in the bottom blowing gas flow rate, the emulsification ratio increases linearly, which increases the mass transfer rate of benzoic acid between water and oil.

关键词： oxygen transfer rate slag-metal reaction cold simulation stirring intensity

Nitrogen acquisition,fixation and transfer in maize/alfalfa intercrops are increased through root contact and morphological responses to interspecies competition

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Journal of Integrative Agriculture 2021年第8期20卷 2240-2254页

作者： SHAO Ze-qiang zheng cong-cong Johannes APOSTMA LU Wen-long GAO Qiang GAO Ying-zhi ZHANG Jin-jing College of Resources and Environmental Sciences Jilin Agricultural University/Key Laboratory of Sustainable Utilization of Soil Resources in the Commodity Grain Bases in Jilin ProvinceChangchun 130118P.R.China College of Resource and Environment Engineering Jilin Institute of Chemical TechnologyJilin 132022P.R.China Forschungszentrum Jülich GmbH Institute of Bio-and Geosciences-Plant Sciences(IBG-2)Jülich 52428Germany Key Laboratory of Vegetation Ecology Northeast Normal UniversityChangchun 130024P.R.China

Nitrogen(N)fixation by legumes and nitrogen transfer to cereals have been considered as important pathways for overyielding and higher N use efficiency in cereal/legume intercropping ***,the extent to which root morphology contributes to N fixation and transfer is unclear.A two-factorial greenhouse experiment was conducted to quantify the N fixation,transfer and root morphology characteristics of the maize/alfalfa intercropping system in two consecutive years using the 15N-urea leaf labeling method,and combining two N levels with three root separation techniques.N application could inhibit N fixation and transfer in a maize/alfalfa intercropping *** of the N application level,compared with plastic sheet separation(PSS),no separation(NS)and nylon mesh separation(NNS)significantly increased the total biomass(36%)and total N content(28%),while the N fixation rate also sharply increased by 75 to 134%,and the amount of N transferred with no root barrier was 1.24–1.42 times greater than that with a mesh *** analysis(RDA)showed that the crown root dry weight(CRDW)of maize and lateral root number(LRN)of alfalfa showed the strongest associations with N fixation and *** results highlight the importance of root contact for the enhancement of N fixation and transfer via changes in root morphology in maize/alfalfa intercropping systems,and the overyielding system was achieved via increases in maize growth,at the cost of smaller decreases in alfalfa biomass production.

关键词： maize/alfalfa intercropping nitrogen fixation and transfer root morphology nitrogen utilization

Hot air treatment activates defense responses and induces resistance against Botrytis cinerea in strawberry fruit

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Journal of Integrative Agriculture 2016年第11期15卷 2658-2665页

作者： JIN Peng zheng cong HUANG Yu-ping WANG Xiao-li LUO Zi-sheng zheng Yong-hua College of Food Science and Technology Nanjing Agricultural University College of Life Science and Food Engineering Huaiyin Institute of Technology College of Biosystems Engineering and Food Science Zhejiang University

The effect of hot air（HA, 45°C, 3.5 h） treatment on reducing gray mold caused by Botrytis cinerea in strawberry fruit and the possible mechanisms were investigated. The results showed that HA treatment significantly reduced lesion diameter and enhanced activities of chitinase（CHI）, β-1,3-glucanase and phenylalanine ammonia-lyase（PAL） in strawberry fruit. Total phenolic contents were also increased by HA treatment. The activities of antioxidant enzymes including superoxide dismutase（SOD）, catalase（CAT） and ascorbate peroxidase（APX） were higher in HA treated strawberry fruit than those in control. Expression of three defense related genes such as CAT, CCR-1 allele and PLA6 was greatly induced in HA treated strawberry fruit with or without inoculation by B. cinerea. In addition, the in vitro experiment showed that HA treatment inhibited spore germination and tube growth of B. cinerea. These results suggested that HA treatment directly activated disease resistance against B. cinerea in strawberry fruit without priming response and directly inhibiting growth of B. cinerea.

关键词： strawberry hot air postharvest disease induced resistance Botrytis cinerea

Changes of paired-pulse evoked responses during the development of epileptic activity in the hippocampus

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第9期12卷 704-711页

作者： Zhou-yan FENG Xiao-jing zheng cong TIAN Yang WANG Hao-yu XING Ministry of Education Key Lab of Biomedical Engineering College of Biomedical Engineering & Instrument ScienceZhejiang UniversityHangzhou 310027China

Dysfunction of inhibitory synaptic transmission can destroy the balance between excitatory and inhibitory synaptic inputs in neurons,thereby inducing epileptic *** aim of the paper is to investigate the effects of successive excitatory inputs on the epileptic activity induced in the absence of ***-pulse orthodromic and antidromic stimulations were used to test the changes in the evoked responses in the ***(PTX),γ-aminobutyric acid(GABA) type A(GABA A) receptor antagonist,was added to block the inhibitory synaptic transmission and to establish the epileptic *** evoked population spike(PS) was recorded in the CA1 region of the *** results showed that the application of PTX induced a biphasic change in the paired-pulse ratio of PS amplitude.A short latency increase of the second PS(PS2) was later followed by a reappearance of PS2 *** type of depression was observed in both orthodromic and antidromic paired-pulse responses,whereas the GABAergic PS2 depression [called paired-pulse depression(PPD)] during baseline recordings only appeared in orthodromic-evoked *** addition,the depression duration at approximately 100 ms was consistent with a relative silent period observed within spontaneous burst discharges induced by prolonged application of *** conclusion,the neurons may ignore the excitatory inputs and intrinsically generate bursts during epileptic *** depolarization block could be the mechanisms underlying the PPD in the absence of GABA A *** distinct neuronal responses to stimulations during different epileptic stages may implicate the different antiepileptic effects of electrical stimulation.

关键词： Epilepsy Paired-pulse depression γ-Aminobutyric acid(GABA) Picrotoxin(PTX) CA1 region In-vivo

ABSTRACTS——From Journal of Iron and Steel Research(in Chinese)

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Journal of Iron and Steel Research International 2002年第2期9卷 1-6页

作者： YANG Li hong YANG Wen yuan zheng cong jie CUI Jian JIANG Xiao fang Central Iron&Steel Research Institute Baoshan Iron and Steel Group Co

The electrochemical nature of reaction between melt and slag in a closed system was worked out. Experimental results demonstrated that both the rate and reaction extent increase when the electronic conductor or voltage was applied between melt and slag. The bigger the contact area of the conductor with melts is, the faster the reaction rate is. With the increase of applied voltage which is beneficial for electron's migration between metal and slags, the rate and extent of reaction increase.

关键词： metal slag reaction electrochemistry electronic conduction

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Characteristics of rock fragments in different forest stony soil and its relationship with macropore characteristics in mountain area, northern China

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Journal of Mountain Science 2018年第3期15卷 519-531页

作者： MENG Chen NIU Jian-zhi YIN zheng-cong LUO Zi-teng LIN Xing-na JIA Jing-wei College of Soil and Water Conservation Beijing Forestry University Beijing Collaborative Innovation Center for Eco-environmental Improvement with Forestry and Fruit Trees Beijing University of Agriculture Department of Geography Texas A&M University College Station

Rock fragments have major effect on soil macropores and water movement. However, the characteristics of rock fragments and their relationship with macropore characteristics remain elusive in forest stony soils in northern mountainous area of China. The objectives of this study are to(1) use Industrial Computed Tomography(CT) scanning to quantitatively analyze rock fragment characteristics in intact soil columns in different forest lands and(2) identify the relationship between characteristics of rock fragments and that of the macropores. Intact soil columns that were 100 mm in diameter and 300 mm long were randomly taken from six local forest stony soils in Wuzuolou Forest Station in Miyun, Beijing. Industrial CT was used to scan all soil column samples, and then the scanned images were utilized to obtain the three-dimensional(3 D) images of rock fragments and macropore structures. Next, theparameters of the rock fragments and macropore structure were measured, including the volume, diameter, surface area, and number of rock fragments, as well as the volume, diameter, surface area, length, angle, tortuosity and number of macropores. The results showed that no significant difference was found in soil rock fragments content in the 10-30 cm layer between mixed forest and pure forest, but in the 0-10 cm soil layer, the rock fragments in mixed forest were significantly less than in pure forest. The number density of macropores has significant negative correlation with the number of rock fragments in the 0-10 cm soil layer, whereas this correlation is not significant in 10-20 cm and 20-30 cm soil layers. The volume density of macropore was not correlated with the volume density of rock fragments, and there is no correlation between the density of macropore surface area and the density of rock fragment surface area. Industrial CT scanning combined with image processing technology canprovide a better way to explore 3 D distribution of rock fragments in soil. The content of

关键词： Rock fragment Macropore Forest stone soil Mountain area Industrial Computed Tomography

Preparation and Characterization of Monoclonal Antibodies against VP1 Protein of Foot-and-mouth Disease Virus O/China99

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Virologica Sinica 2009年第6期24卷 566-572页

作者： Shuai SONG Tong LIN Jun-jun SHAO Shan-dian GAO Guo-zheng cong Jun-zheng DU Hui-yun CHANG National FMD Reference Laboratory Key Laboratory of Animal Virology of Ministry of Agriculture State Key Laboratory of veterinary Etiological Biology Lanzhou Veterinary Research Institute Chinese Academy of Agricutral Science Lanzhou 730046 China Guangdong Open Laboratory of Veterinary Public Healty Institute of Veterinary Medicine Guangdong Academy of Agricultural Science Guangzhou 510640 China

Monoclonal antibodies (McAbs) 1A9 and 9F12 against Foot-and-mouth disease virus (FMDV) serotype O were produced by fusing SP2/0 myeloma cells with splenocyte from the mouse immunized with O/China99. Both McAbs reacted with O/China99 but not with Asia 1, as determined by immunohistochemistry assay. The microneutralization titer of the McAbs 1A9 and 9F12 were 640 and 1 280, respectively. Both McAbs contain kappa light chains, but the McAbs 1A9 and 9F12 were IgG1 and IgM, respectively. In order to define the McAbs binding epitopes, the reactivity of these McAbs against VP1, P20 and P14 were examined using indirect ELISA, the result showed that both McAbs reacted with VP1 and P20. McAbs may be used for further studies of vaccine, diagnostic methods, prophylaxis, etiological and immunological researches on FMDV.

关键词： Foot-and-mouth disease virus (FMDV) Monoclonal antibody Neutralizing activity VP1 protein

Develope Monoclonal Antibody against Foot-and-mouth Disease Virus A Type

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Virologica Sinica 2011年第4期26卷 273-278页

作者： Tong Lin Jing Li Jun-jun Shao Guo-zheng cong Jun-zheng Du Shan-dian Gao Hui-yun Chang State Key Laboratory of Veterinary Etiological Biology National Foot and Mouth Disease Reference Laboratory Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences Lanzhou 730046 China

In order to develop an anti-FMDV A Type monoclonal antibody （mAb）, BABL/c mice were immunized with FMDV A type. Monoclonal antibodies （mAbs） 7B 11 and 8H4 against Foot-and-mouth disease virus （FMDV） serotype A were produced by fusing SP2/0 myeloma cells with splenocyte from the mouse immunized with A/AV88. The microneutralization titer of the mAbs 7Bll and 8H4 were 1024 and 512, respectively. Both mAbs contain kappa light chainS, the mAbs were IgG1. In order to define the mAbs binding epitopes, the reactivity of these mAbs against A Type FMDV, were examined using indirect ELISA, the result showed that both mAbs reacted with A Type FMDV. These mAbs may be used for further vaccine studies, diagnostic methods, prophylaxis, etiological and immunological research on FMDV. Characterization of these ncindicated that prepared anti-FMDV A mAbs had no cross-reactivity with Swine Vesicular Disease （SVD） or FMDV O, Asial and C Type antigens. Their titers in abdomen liquor were 1：5×10^6 and 1：2×10^6, respectively. 7B 11 was found to be of subtype IgGb 8H4 was classified as IgG2b subtype. The mAbs prepared in this study, are specific for detection of FMDV serotype A, and is potentially useful for pen-side diagnosis.

关键词： Foot-and-mouth disease virus (FMDV) A Type Monoclonal antibody (mAb) Neutralizing activity

Application of VP1 Protein to Develop Monoclonal Antibody against Foot-and-mouth Disease Virus Asia1 Type

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Virologica Sinica 2009年第3期24卷 215-220页

作者： Tong LIN Jun-zheng DU Jun-jun SHAO Guo-zheng cong Shuai SONG Shan-dian GAO Hui-yun CHANG Key Laboratory of Animal Virology of Ministry of Agriculture State Key Laboratory of Veterinary Etiological Biology Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Sciences Lanzhou 730046 China

In order to develop an anti-FMDV Asial type monoclonal antibody （mAb）, BABL/c mice were immunized with recombinant FMDV VP1 protein. Three mAbs, 1B8, 5El and 5E2, were then further optimized. The result indicated that prepared anti-FMDV Asial mAbs had no cross-reactivity with Swine vesicular disease （SVD） and FMDV O, A and C type antigen. Their titers in abdomen liquor were 1：5×10^6, 1：2×10^6 and 1：5×10^6, respectively. 1B8 was found to be of IgG1 subtype, 5El and 5E2 belonged to IgG2b subtype. In this study, the prepared mAbs are specific for detecting FMDV type Asia1, and is potentially useful for pen-side diagnosis.

关键词： Asial type FMDV VP1 protein Monoclonal antibody