Develope Monoclonal Antibody against Foot-and-mouth Disease Virus A Type

作     者：Tong Lin Jing Li Jun-jun Shao Guo-zheng Cong Jun-zheng Du Shan-dian Gao Hui-yun Chang 

作者机构：State Key Laboratory of Veterinary Etiological Biology National Foot and Mouth Disease Reference Laboratory Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences Lanzhou 730046 China 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2011年第26卷第4期

页      面：273-278页

核心收录：

学科分类：090603[农学-临床兽医学] 090602[农学-预防兽医学] 09[农学] 0906[农学-兽医学] 

基　　金：State Key Projects of Transgene Program(2011ZX08011-004 2009ZX 08007- 008B 2009ZX08006-002B) 

主　　题：Foot-and-mouth disease virus (FMDV) A Type Monoclonal antibody (mAb) Neutralizing activity 

摘      要：In order to develop an anti-FMDV A Type monoclonal antibody （mAb）, BABL/c mice were immunized with FMDV A type. Monoclonal antibodies （mAbs） 7B 11 and 8H4 against Foot-and-mouth disease virus （FMDV） serotype A were produced by fusing SP2/0 myeloma cells with splenocyte from the mouse immunized with A/AV88. The microneutralization titer of the mAbs 7Bll and 8H4 were 1024 and 512, respectively. Both mAbs contain kappa light chainS, the mAbs were IgG1. In order to define the mAbs binding epitopes, the reactivity of these mAbs against A Type FMDV, were examined using indirect ELISA, the result showed that both mAbs reacted with A Type FMDV. These mAbs may be used for further vaccine studies, diagnostic methods, prophylaxis, etiological and immunological research on FMDV. Characterization of these ncindicated that prepared anti-FMDV A mAbs had no cross-reactivity with Swine Vesicular Disease （SVD） or FMDV O, Asial and C Type antigens. Their titers in abdomen liquor were 1：5×10^6 and 1：2×10^6, respectively. 7B 11 was found to be of subtype IgGb 8H4 was classified as IgG2b subtype. The mAbs prepared in this study, are specific for detection of FMDV serotype A, and is potentially useful for pen-side diagnosis.