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转录组分析调控桃采后冷害和硬度的候选主效基因

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农业生物技术学报 2024年第4期32卷 751-761页

作者：张志刚陈玉峰王长江徐晋姜延伟郑亚琴刘振宁陈守兵刘一华徐蒙临沂大学农林科学学院邹城市自然资源和规划局

冷敏性果实采后低温贮藏容易造成果实冷害，程序性降温(low-temperature conditioning, LTC)处理在减缓桃(Prunus persica)等冷敏性果实冷害中具有显著效果，但是贮藏前期果实会发生轻微软化。本研究通过加权基因共表达网络分析(weighte... 详细信息

冷敏性果实采后低温贮藏容易造成果实冷害，程序性降温(low-temperature conditioning, LTC)处理在减缓桃(Prunus persica)等冷敏性果实冷害中具有显著效果，但是贮藏前期果实会发生轻微软化。本研究通过加权基因共表达网络分析(weighted gene co-expression network analysis, WGCNA)发现了与冷害和硬度表型高度相关的基因模块。分别对模块基因进行主效转录因子鉴定和调控网络构建，发现桃WRKY转录因子PpWRKY43和生长素响应因子(auxin response factor, ARF) PpARF1可能通过调控冷调节蛋白(cold-regulated protein, COR) PpCOR413-2和脂氧合酶(lipoxygenase 5, LOX5) PpLOX5参与果实采后冷害调控，NAC转录因子PpNAC5可能通过调控桃果胶裂解酶2(P. persica pectate lyase 2, PpePL2)参与桃果实采后软化。本研究结果为桃果实采后保鲜减损研究提供基础资料。

关键词：桃采后冷害加权基因共表达网络分析(WGCNA) 硬度

Serum hepatitis B virus RNA is a predictor of HBeAg seroconversion and virological response with entecavir treatment in chronic hepatitis B patients

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World Journal of Gastroenterology 2019年第6期25卷 719-728页

作者： Hao Luo Xia-Xia Zhang Li-hua Cao Ning Tan Qian Kang Hong-Li Xi Min Yu Xiao-yuan Xu Department of Infectious Diseases Peking University First Hospital Department of Gastroenterology Capital Medical University Beijing Tiantan Hospital Department of Infectious Diseases the Third Hospital of Qinhuangdao

BACKGROUND Characteristics of alterations of serum hepatitis B virus(HBV) RNA in different chronic hepatitis B(CHB) patients still cannot be fully explained. Whether HBV RNA can predict HBeAg seroconversion is still *** To investigate whether HBV RNA can predict virological response or HBeAg seroconversion during entecavir(ETV) treatment when HBV DNA is *** The present study evaluated 61 individuals who were diagnosed and treated with long-term ETV monotherapy at the Department of Infectious Diseases of Peking University First Hospital(China) from September 2006 to December ***, 30 treatment-naive individuals were included. Serum HBV RNA were extracted from 140 μL serum samples at two time points. Then they were reverse transcribed to cDNA with the HBV-specific primer. The product was quantified by real-time quantitative PCR(RT-PCR) using TAMARA probes. Statistical analyses were performed with IBM SPSS *** Level of serum HBV RNA at baseline was 4.15 ± 0.90 log10 copies/mL. HBV RNA levels showed no significant difference between the virological response(VR)and partial VR(PVR) groups at baseline(P = 0.940). Serum HBV RNA significantly decreased among patients who achieved a VR during ETV therapy(P < 0.001). The levels of HBV RNA in both HBeAg-positive patients with seroconversion group and those with no seroconversion increased after 24 wk of treatment. Overall, HBV RNA significantly but mildly correlated to HBsAg(r =0.265, P = 0.041), and HBV RNA was not correlated to HBV DNA(r = 0.242, P =0.062). Furthermore, serum HBV RNA was an independent indicator for predicting HBeAg seroconversion and virological response. HBeAg seroconversion was more likely in CHB patients with HBV RNA levels below4.12 log10 copies/mL before *** The level of serum HBV RNA could predict HBeAg seroconversion and PVR during treatment. In the PVR group, the level of serum HBV RNA tends to be increasing.

关键词： Chronic hepatitis B Hepatitis B virus RNA Virological response HBeAg seroconversion Entecavir

Detachment-Independent Cationic-Dipeptide Beacons:Reduced False-Negative Signal and Accelerated Fluorescent Lighting in Cell Imaging

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Journal of Analysis and Testing 2022年第1期6卷 19-26页

作者： Guang-Wei Fu Guo-Yan Luo Xiao-Xuan Li zhen Zou Yan-Li Lei Zhi-He Qing Jue-Wen Liu Rong-hua Yang Hunan Provincial Key Laboratory of Cytochemistry School of Chemistry and Chemical EngineeringChangsha University of Science and TechnologyChangsha 410114China College of Chemistry and Chemical Engineering Hunan Normal UniversityChangsha 410081China Department of Chemistry Waterloo Institute for NanotechnologyUniversity of WaterlooWaterlooON N2L 3G1Canada

Nanocarriers have been developed to deliver DNA probes into cells for imaging analysis and improving their nuclease ***,we found obvious lowered sensitivity and even false-negative results in the traditional DNA detachment-dependent fluorescent lighting ***,we developed a detachment-independent fluorescent lighting mechanism integrated with cationic dipeptide nanoparticles(CDNs).CDNs displayed little quenching effect on fluorophores labeled on probes and improved the nuclease resistance of *** contrast to the traditional beacon,the fluorescence lighting was significantly accelerated without the need of desorption of the recognition products from the nanosurface,avoiding false-negative results from non-specific conformational *** work will open up new thoughts to improve the sensing performance of DNA probes in cell imaging,such as speed and sensitivity.

关键词： Cationic dipeptide nanoparticles DNA probe False-negative signal Fluorescence Cell imaging

Discharge characteristic of very high frequency capacitively coupled argon plasma

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Chinese Physics B 2021年第9期30卷 329-334页

作者： Gui-Qin Yin Jing-Jing Wang Shan-Shan Gao Yong-Bo Jiang Qiang-hua yuan Key Laboratory of Atomic and Molecular Physics&Functional Materials of Gansu Province College of Physics and Electronic EngineeringNorthwest Normal UniversityLanzhou 730070China

The discharge characteristics of capacitively coupled argon plasmas driven by very high frequency discharge are *** mean electron temperature and electron density are calculated by using the Ar spectral lines at different values of power(20 W-70 W)and four different frequencies(13.56 MHz,40.68 MHz,94.92 MHz,and 100 MHz).The mean electron temperature decreases with the increase of power at a fixed *** mean electron temperature varies non-linearly with frequency increasing at constant *** 40.68 MHz,the mean electron temperature is the *** electron density increases with the increase of power at a fixed *** the cases of driving frequencies of 94.92 MHz and 100 MHz,the obtained electron temperatures are almost the same,so are the electron ***-in-cell/Monte-Carlo collision(PIC/MCC)method developed within the Vsim 8.0 simulation package is used to simulate the electron density,the potential distribution,and the electron energy probability function(EEPF)under the experimental *** sheath width increases with the power *** EEPF of 13.56 MHz and 40.68 MHz are both bi-Maxwellian with a large population of low-energy *** EEPF of 94.92 MHz and 100 MHz are almost the same and both are nearly Maxwellian.

关键词： very high frequency discharges capacitively coupled plasma particle-in-cell/Monte-Carlo collisions

Characterization of dual enzyme resulted from bicistronic expression of two β-glucanases in porcine cells

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Journal of Integrative Agriculture 2015年第4期14卷 732-740页

作者： ZHANG Xian-wei LI Zi-cong MENG Fan-ming WANG De-hua LIU De-wu HE Xiao-yan SUN Yue BAI Yin-shan WU zhen-fang National Engineering Research Center for Breeding Swine Industry South China Agricultural University Wen's Research Institute Guangdong Wen's Food Group Co.Ltd.

Many animal feed grains contain high β-glucan in the cell wall. Pigs do not secret β-glucanase to degrade the β-glucan in their feed. The indigestible β-glucan not only blocks the release of nutrients from the grain cell wall, but also increases the digesta viscosity in the gastrointestinal tract of pigs. Therefore, dietary β-glucan significantly inhibits nutrient digestion and absorption in pigs. Transgenic expression of β-glucanase in the digestive tract of pigs may offer a solution to solve this problem. In the current study, four artificial codon-optimized β-glucanases genes was prepared and expressed in porcine cells. Only p Bg A and p Egx showed high activity in transfected pig kidney cells. To improve the p H range and p H stability of β-glucanase, the two β-glucanases, p Bg A and p Egx, were co-expressed in pig kidney cells and salivary gland cells by Linker A3 or 2A peptide. The resulting dual enzymes of p Bg A3 p Eg and p Bg2 Ap Eg showed significantly enlarged p H range and significantly increased p H stability, as compared to parental enzymes. These results provide useful data for future study on increasing the feed digestibility of pigs by transgenic expression of β-glucanase in their salivary glands.

关键词： β-glucanase bicistronic pig feed digestibility salivary gland cells transgenic

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不同形态硫对木榄吸收土壤汞的影响

生态学杂志 2016年第6期35卷 1525-1530页

作者：袁俊张玲靳明华丁振华厦门大学环境与生态学院福建厦门361100

硫在汞(Hg)的生物地球化学循环中发挥着重要作用。为了解不同价态硫(S、Na_2SO_4、Na_2S)对红树植物吸收Hg的影响,本文以红树植物木榄(Bruguiera gymnorrhiza(L.)Lamk)为研究对象,在受汞污染土壤中添加不同价态(S、Na_2SO_4、Na_2S)和浓... 详细信息

硫在汞(Hg)的生物地球化学循环中发挥着重要作用。为了解不同价态硫(S、Na_2SO_4、Na_2S)对红树植物吸收Hg的影响,本文以红树植物木榄(Bruguiera gymnorrhiza(L.)Lamk)为研究对象,在受汞污染土壤中添加不同价态(S、Na_2SO_4、Na_2S)和浓度(0、0.1%、1%)的硫,通过土培实验,研究木榄各器官中总Hg和Me Hg的富集特征以及硫对植物吸收汞和甲基汞的影响。结果显示:3种不同价态的硫及其化合物能够提高木榄茎对Hg的吸收,但对根和叶吸收Hg的影响比较复杂且无规律性。硫形态对木榄吸收Me Hg的影响主要在于根部,施硫能提高根中Me Hg的含量。在根部中,硫含量与Hg、Me Hg存在极显著性的正相关性(P0.05)。

关键词：木榄汞甲基汞硫相关性

Application of multi-component joint inversion in oil and gas exploration:A case study of reservoir and gas saturation prediction of the Xujiahe formation in the PLN area of the central Sichuan Basin

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Applied Geophysics 2020年第5期17卷 879-889,905页

作者： Wang Dong He zhen-hua Wang Xu-Ben Li Le Yang Hai-Tao Fu Zhi-Guo Wang Hong-Yan The college of geophysics Chengdu University of TechnologyChengdu 610059China BGP Inc. China National Petroleum CorporationZhuozhou 072750China Mountain Geophysical Technology Test Center CNPC Chengdu 610213China

Multi-component seismic exploration is an important technique in the utilization of P-waves and converted S-waves for oil and gas *** has unique advantages in the structural imaging of gas zones,reservoir prediction,lithology,and gas-water identifi cation,and the development direction and degree of ***-component joint inversion is one of the most important steps in multi-component *** this paper,starting from the basic principle of multi-component joint inversion,the diff erences between the method and single P-wave inversion are ***,the technique is applied to the PLN area of the Sichuan Basin,and the P-wave impedance,S-wave impedance,and density are obtained based on multi-component joint *** the velocity and lithology,porosity,and gas saturation fi tting formulas,prediction results are calculated,and the results are ***,multi-component joint inversion and single P-wave inversion are compared in eff ective reservoir *** results show that multi-component joint inversion increases the constraints on the inversion conditions,reduces the multi-solution of a single P-wave inversion,and is more objective and reliable for the identification of reservoirs,effectively improving the accuracy of oil and gas reservoir prediction and development.

关键词： multi-component joint inversion lithology identifi cation porosity gas saturation reservoir prediction

湖南省细菌耐药监测网2012-2021年血标本分离细菌耐药性监测报告

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中国感染控制杂志 2024年第8期23卷 921-931页

作者：袁红霞蒋静陈丽华付陈超李晨李艳明宁兴旺刘君石国民唐曼娟邬靖敏杨怀德郑铭周杰英任南吴安华黄勋郴州市第一人民医院检验医学中心湖南郴州423000 中南大学湘雅三医院检验科湖南长沙410013 中南大学湘雅医院医院感染控制中心湖南长沙410008 湖南省细菌耐药监测网办公室湖南长沙410008 浏阳市中医医院检验科湖南浏阳410300 中南大学湘雅医院检验科湖南长沙410008 湖南中医药大学第一附属医院医学检验与病理中心湖南长沙410011 湘潭市中心医院检验科湖南湘潭411100 长沙市中心医院检验科湖南长沙410004 长沙市第一医院检验科湖南长沙410005 张家界市人民医院检验科湖南张家界427000 湖南省医院感染管理质量控制中心湖南长沙410008 国家老年疾病临床医学研究中心(湘雅医院) 湖南长沙410008

目的了解湖南省血标本分离细菌的分布及耐药性变迁,为临床血流感染初始经验诊治提供依据。方法收集湖南省细菌耐药监测网2012—2021年各成员单位上报数据,细菌耐药监测方法参照全国细菌耐药监测网(CARSS)技术方案执行,应用WHONET 5.6软... 详细信息

目的了解湖南省血标本分离细菌的分布及耐药性变迁,为临床血流感染初始经验诊治提供依据。方法收集湖南省细菌耐药监测网2012—2021年各成员单位上报数据,细菌耐药监测方法参照全国细菌耐药监测网(CARSS)技术方案执行,应用WHONET 5.6软件和SPSS 27.0软件对血标本分离细菌及药敏试验结果进行分析。结果2012—2021年湖南省细菌耐药监测网成员单位血标本共分离细菌207054株,其中革兰阳性菌107135株(51.7%)、革兰阴性菌99919株(48.3%);2012—2021年居前6位的病原菌无变化,居首位的为大肠埃希菌(51537株,24.9%),其次分别为表皮葡萄球菌(29115株,14.1%)、金黄色葡萄球菌(17402株,8.4%)、肺炎克雷伯菌(17325株,8.4%)、铜绿假单胞菌(4010株,1.9%)和鲍曼不动杆菌(3598株,1.7%)。耐甲氧西林金黄色葡萄球菌(MRSA)的检出率由2015年的30.3%下降至2021年的20.7%,耐甲氧西林凝固酶阴性葡萄球菌(MRCNS)的检出率呈逐年上升趋势(57.9%~66.8%)。未发现对万古霉素、利奈唑胺和替考拉宁耐药的葡萄球菌。革兰阴性菌中,大肠埃希菌、肺炎克雷伯菌构成比分别为43.9%~53.9%、14.2%~19.5%,呈上升趋势(均P<0.001);铜绿假单胞菌、鲍曼不动杆菌的构成比分别为3.6%~5.1%、3.0%~4.5%,呈逐年下降趋势(均P<0.001)。2012—2021年大肠埃希菌对亚胺培南、厄他培南的耐药率分别为1.0%~2.0%、0.6%~1.1%,呈下降趋势(均P<0.001)。肺炎克雷伯菌对美罗培南、厄他培南的耐药率分别为7.4%~13.7%、4.8%~6.4%,呈下降趋势(均P<0.001)。铜绿假单胞菌、鲍曼不动杆菌对碳青霉烯类抗生素的耐药率分别为7.1%~15.6%、34.7%~45.7%,对碳青霉烯类抗生素的耐药性趋势相对稳定,但与2012—2016年相比均有下降。2012—2021年大肠埃希菌对第三代头孢菌素的耐药率为41.0%~65.4%,呈逐年下降趋势。结论湖南省血标本革兰阴性杆菌构成比逐年增高,耐碳青霉烯类革兰阴性杆菌的检出率近5年相对稳定,凝固酶阴性葡萄球菌检出率呈下降趋势。

关键词：血培养临床分离菌病原菌血流感染耐药性湖南省细菌耐药监测网

不同光环境照度下平板电脑屏幕亮度与观看适宜性主观评价实验研究

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中国照明电器 2024年第5期 55-59页

作者：钟浩袁安朋罗震黎嘉诚蔡纯郭滨刚蒋婷权薇深圳市计量质量检测研究院广东深圳518055 深圳市联控智能科技有限公司广东深圳518002 深圳市光科全息技术有限公司广东深圳518052

平板电脑普遍具有适宜亮度随光环境亮度自动调整的功能,不适宜的屏幕亮度容易让人眼产生视疲劳,甚至影响视觉健康。本文通过组织志愿者对不同光环境照度下平板电脑适宜亮度的眼睛适宜性主观感受适宜范围进行了研究,得出人眼在不同光环... 详细信息

平板电脑普遍具有适宜亮度随光环境亮度自动调整的功能,不适宜的屏幕亮度容易让人眼产生视疲劳,甚至影响视觉健康。本文通过组织志愿者对不同光环境照度下平板电脑适宜亮度的眼睛适宜性主观感受适宜范围进行了研究,得出人眼在不同光环境照度下的可舒适观看平板电脑的适宜亮度适宜范围限值,为相关方评价平板电脑产品的显示亮度适宜性提供技术依据。

关键词：平板电脑屏幕显示亮度适宜范围视觉健康

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