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Journal of Computer Science & Technology 2021年第2期36卷 361-374页

作者： Xia-An Bi zhao-xu xing Rui-Hui xu Xi Hu College of Information Science and Engineering Hunan Normal UniversityChangsha 410006China Hunan Provincial Key Laboratory of Intelligent Computing and Language Information Processing Hunan Normal UniversityChangsha 410006China

As an emerging research field of brain science,multimodal data fusion analysis has attracted broader attention in the study of complex brain diseases such as Parkinson's disease(PD).However,current studies primarily lie with detecting the association among different modal data and reducing data *** data mining method after fusion and the overall analysis framework are *** this study,we propose a weighted random forest(WRF)model as the feature screening *** interactions between genes and brain regions are detected as input multimodal fusion features by the correlation analysis *** implement sample classification and optimal feature selection based on WRF,and construct a multimodal analysis framework for exploring the pathogenic factors of *** experimental results in Parkinson's Progression Markers Initiative(PPMI)database show that WRF performs better compared with some advanced methods,and the brain regions and genes related to PD are *** fusion of multi-modal data can improve the classification of PD patients and detect the pathogenic factors more comprehensively,which provides a novel perspective for the diagnosis and research of *** also show the great potential of WRF to perform the multimodal data fusion analysis of other brain diseases.

关键词： multimodal fusion feature Parkinson's disease pathogenic factor detection sample classification weighted random forest model

牦牛妊娠早、中期胎盘组织中STAB1的表达、分布及功能分析

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农业生物技术学报 2024年第4期32卷 785-794页

作者：魏倩倩张全伟张博皓白旭赵兴绪张勇甘肃农业大学生命科学技术学院甘肃省动物生殖生理及繁殖调控重点实验室甘肃农业大学动物医学院

哺乳动物胎盘发育对子宫内环境调节及妊娠维持至关重要。胎盘发育不良或异常可引发多种妊娠疾病。稳定素蛋白-1(stabilin-1, STAB1)参与调节动物多个器官发育，但在牦牛(Bos grunniens)胎盘中的作用及调节机制尚不完全清楚。本研究旨在... 详细信息

哺乳动物胎盘发育对子宫内环境调节及妊娠维持至关重要。胎盘发育不良或异常可引发多种妊娠疾病。稳定素蛋白-1(stabilin-1, STAB1)参与调节动物多个器官发育，但在牦牛(Bos grunniens)胎盘中的作用及调节机制尚不完全清楚。本研究旨在探究牦牛妊娠早、中期胎盘组织中STAB1分布、表达规律及其潜在功能。采集牦牛妊娠早、中期胎盘组织，通过苏木精-伊红(hematoxylin-eosin, HE)染色、免疫组织化学(immunohistochemistry, IHC)染色、免疫荧光(immunofluorescence, IF)染色、qRT-PCR和Western blot研究牦牛妊娠早、中期胎盘组织中STAB1表达特点及分布规律；利用数据非依赖性模式(dataindependent acquisition, DIA)蛋白质组学数据，预测分析牦牛胎盘中STAB1互作蛋白及潜在生物学功能。结果表明，STAB1蛋白主要定位于牦牛胎盘滋养层巨细胞和单核滋养层细胞胞质；与妊娠早期胎盘相比，牦牛妊娠中期胎盘组织中STAB1基因及蛋白表达显著下调(P<0.05)；生物信息学分析显示，STAB1可能通过细胞黏附、钙离子结合和质膜组成等途径调控牦牛妊娠早、中期胎盘动态发育。综上，STAB1通过调节牦牛胎盘滋养层细胞的功能参与妊娠早、中期胎盘发育和妊娠维持。本研究为STAB1在牦牛胎盘发育中的功能及机制提供参考。

关键词：胎盘稳定素蛋白-1(STAB1) DIA蛋白质组学生物信息学分析

Comparative Proteomic Analysis of Plasma from Clinical Healthy Cows and Mastitic Cows

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Agricultural Sciences in China 2009年第10期8卷 1263-1269页

作者： YANGYong-xin zhao xing-xu ZHANG Yong College of Veterinary Medicine Gansu Agricultural University Lanzhou 730070 P.R.China Institute of Animal Husbandry and Veterinary Medicine Anhui Academy of Agricultural Sciences Hefei 230031 P.R. China

The current research presents the protein changes in plasma from healthy dairy cows and clinical mastitic cows using two- dimensional gel electrophoresis （2-DE）. After staining with silver nitrate and Coomassie Blue, differential expression proteins were detected by PDQuest 7.4 software, and then subjected to ion trap mass spectrometer equipped with a Surveyor HPLC System, differential spots of protein were identified. Three protein spots that originated from preparation gels were identified to be two proteins. Overall, haptoglobin precursor was up-regulated in cows infected with clinical mastitis and could be a mastitis-associated diagnostic marker, whereas SCGB 2A1 （secretoglobin, family 2A, member 1） was down-regulated protein. Plasma protein expression patterns were changed when cows were infected with mammary gland inflammation; it suggests that analysis of differential expression protein might be useful to clarify the mechanisms involved in the pathophysiology, and find new diagnostic markers of mastitis and potential protein targets for treatment.

关键词： dairy cows clinical mastitis plasma proteome

牦牛STAG3基因克隆及其在不同年龄段牦牛睾丸中的表达定位

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农业生物技术学报 2024年第2期32卷 344-354页

作者：石军陈文丽白旭李建富袁宝赵兴绪张全伟甘肃农业大学生命科学技术学院甘肃省动物生殖生理及繁殖调控重点实验室甘肃农业大学动物医学院

基质抗原3(stromal antigen 3, STAG3)是黏连蛋白复合体的重要成分之一，在动物卵泡发育、卵巢早衰和癌症发生等生物学过程中发挥重要调节作用，其在牦牛(Bos grunniens)生殖和发育过程中的作用尚不明确。本研究以不同岁龄(2, 4, 6和8岁... 详细信息

基质抗原3(stromal antigen 3, STAG3)是黏连蛋白复合体的重要成分之一，在动物卵泡发育、卵巢早衰和癌症发生等生物学过程中发挥重要调节作用，其在牦牛(Bos grunniens)生殖和发育过程中的作用尚不明确。本研究以不同岁龄(2, 4, 6和8岁龄)牦牛睾丸组织为材料，通过反转录PCR(reverse transcription-PCR, RT-PCR)克隆STAG3基因编码区序列(CDS)，利用STRING蛋白质互作数据库(STRING:functional protein association networks)和基因本体(Gene Ontology, GO)预测分析STAG3互作蛋白及生物学功能；通过苏木精-伊红(hematoxylin-eosin, HE)染色观察不同年龄牦牛睾丸组织形态结构；免疫组织化学(immunohistochemistry, IHC)染色和qRT-PCR分析STAG3分布及表达规律。结果显示，牦牛STAG3基因CDS区全长3 679 bp，开放阅读框为1 659 bp，编码552个氨基酸，蛋白无跨膜结构区，可能参与第一次减数分裂、联会复合和染色体浓缩等生物学过程；STAG3蛋白在各年龄段牦牛睾丸组织中均有表达，主要定位于初级精母细胞和次级精母细胞；2岁龄牦牛睾丸组织STAG3表达最高，且随年龄增长表达逐渐降低。结果表明，STAG3在动物进化中较为保守，各年龄段牦牛睾丸组织均有表达，其可能在牦牛生殖过程中发挥重要作用。本研究为解析牦牛生殖过程中STAG3功能及调控机制提供了参考依据。

关键词：牦牛基质抗原3(STAG3) 基因克隆生物信息学分析

芍药苷对乳腺炎小鼠乳腺组织氧化应激损伤的信号通路研究

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中国预防兽医学报 2024年第8期46卷 847-855页

作者：刘馨鸿郭超宋冰白敏汪湛东赵泓彰王琼张延英赵兴绪甘肃农业大学动物医学院甘肃兰州730070 甘肃中医药大学甘肃兰州730000 甘肃省实验动物行业技术中心甘肃兰州730000

为探究芍药苷通过何种通路对乳腺炎小鼠氧化应激损伤的保护作用及作用机制,本研究选取产后6 d的BALB/c雌鼠,随机分为阴性对照组、模型组、芍药苷低剂量组(10 mg/kg)、芍药苷中剂量组(20 mg/kg)、芍药苷高剂量组(40 mg/kg)和地塞米松阳... 详细信息

为探究芍药苷通过何种通路对乳腺炎小鼠氧化应激损伤的保护作用及作用机制,本研究选取产后6 d的BALB/c雌鼠,随机分为阴性对照组、模型组、芍药苷低剂量组(10 mg/kg)、芍药苷中剂量组(20 mg/kg)、芍药苷高剂量组(40 mg/kg)和地塞米松阳性对照组(5 mg/kg),每组10只,除阴性对照组外,其余小鼠均采用乳导管注入脂多糖(LPS)的方法建立乳腺炎模型,分别于造模前14 h、2 h以及造模后6 h对小鼠给药干预。造模结束24 h后剖检各组小鼠,观察乳腺组织形态变化,制备各组小鼠乳腺组织病理切片观察其病理变化。结果显示,与阴性对照组相比,模型组小鼠乳区红肿并伴有出血点,乳腺组织病变观察发现乳腺腺泡细胞内炎性细胞浸润明显,腺泡形态改变,表明乳腺炎小鼠模型可用于后续实验。取各组小鼠乳腺组织,采用荧光探针法测定小鼠乳腺组织中活性氧自由基(ROS)含量,采用免疫荧光组织化学技术测定小鼠乳腺组织中4-羟基壬烯酸(4-HNE)蛋白的表达水平,采用生化试剂盒检测小鼠乳腺组织中超氧化物歧化酶(SOD)、丙二醛(MDA)及谷胱甘肽过氧化物酶(GSH-Px)的含量,采用荧光定量PCR(RT-qPCR)和western blot分别检测小鼠乳腺组织中Nrf2信号通路关键分子核因子-红细胞2相关因子2(Nrf 2)、血红素氧合酶1(HO-1)、谷氨酸-半胱氨酸连接酶(GCLC)、醌氧化还原酶(NQO1)mRNA的转录及蛋白表达水平。结果显示,与阴性对照组相比,模型组小鼠乳腺组织中ROS、MDA含量及4-HNE蛋白表达水平均极显著升高(P<0.01),GSH-Px和SOD酶活性及含量均极显著降低(P<0.01),Nrf2、HO-1、GCLC和NQO1 mRNA的转录水平均显著或极显著上调(P<0.05或P<0.01),其蛋白表达水平与mRNA转录水平趋势基本一致;与模型组相比,各给药组小鼠乳腺组织病理损伤明显减轻,小鼠乳腺组织中ROS、MDA含量以及4-HNE蛋白表达水平显著或极显著降低(P<0.05或P<0.01),GSH-Px、SOD含量极显著或显著升高(P<0.01或P<0.05);Nrf2、HO-1、GCLC和NQO1mRNA的转录水平均显著或极显著上调(P<0.05或P<0.01),其蛋白表达水平与mRNA转录水平趋势基本一致。综上所述,本研究证实芍药苷能够治疗小鼠乳腺炎及小鼠乳腺组织氧化损伤,且高剂量芍药苷的治疗作用最佳,其具体作用机制与芍药苷显著激活宿主细胞中Nrf2信号通路拮抗氧化应激损伤有关,该结果为芍药苷在动物乳腺炎预防和治疗中的应用提供了科学依据。

关键词：乳腺炎芍药苷 Nrf2信号通路氧化应激

妊娠中期和后期牦牛胎盘组织中ELANE表达、定位及功能分析

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农业生物技术学报 2024年第10期32卷 2275-2284页

作者：陈文丽张博皓李建富袁宝周斌张勇赵兴绪张全伟甘肃农业大学生命科学技术学院甘肃省动物生殖生理及繁殖调控重点实验室甘肃农业大学动物医学院

哺乳动物妊娠和分娩过程与胎盘炎症发生和发展密切，炎症反应过早或免疫失衡可导致妊娠终止或流产。中性粒细胞弹性蛋白酶(elastase, neutrophil expressed, ELANE)在炎症发生和发展过程中发挥重要作用。本研究旨在探究妊娠中期和后期牦... 详细信息

哺乳动物妊娠和分娩过程与胎盘炎症发生和发展密切，炎症反应过早或免疫失衡可导致妊娠终止或流产。中性粒细胞弹性蛋白酶(elastase, neutrophil expressed, ELANE)在炎症发生和发展过程中发挥重要作用。本研究旨在探究妊娠中期和后期牦牛(Bos grunniens)胎盘组织中ELANE定位、表达规律及潜在功能。采集妊娠中期和后期牦牛胎盘组织(n=3/组)，通过苏木精-伊红(hematoxylin-eosin, HE)染色、免疫组织化学(immunohistochemistry, IHC)染色、免疫荧光(immunofluorescence, IF)染色、实时荧光定量PCR (real-time fluorescence quantitative PCR, qRT-PCR)和蛋白免疫印迹(Western blot)分析ELANE的定位及表达；基于数据非依赖型模式(data-independent acquisition, DIA)蛋白质组学数据预测牦牛胎盘中ELANE潜在生物学功能。HE显示妊娠中期牦牛胎盘组织可见大量滋养层巨细胞和单核滋养层细胞；妊娠后期滋养层细胞数目减少。IHC和IF表明，ELANE蛋白主要定位于牦牛胎盘滋养层巨细胞和单核滋养层细胞胞质。qRT-PCR和Western blot显示，与妊娠中期相比，妊娠后期牦牛胎盘组织ELANE基因及其蛋白表达显著上调(P<0.01)。生物信息学分析发现，ELANE可能通过吞噬作用、细胞外空间的组成、丝氨酸型内肽酶活性和与细胞因子结合等途径调控妊娠中期和后期牦牛胎盘的生长发育。综上，ELANE可能通过调节牦牛胎盘滋养层细胞的功能调控胎盘动态发育和妊娠维持。本研究为深入探讨牦牛胎盘组织ELANE功能提供参考。

关键词：胎盘中性粒细胞弹性蛋白酶(ELANE) 表达定位数据非依赖型模式(DIA)蛋白质组学

Differential Proteomic Analysis of Carbon Ion Radiation in Sheep Sperm

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Journal of Integrative Agriculture 2013年第9期12卷 1629-1637页

作者： HE Yu-xuan LI Hong-yan ZHANG Yong HE Jian-hua ZHANG Hong zhao xing-xu College of Veterinary Medicine Gansu Agricultural University Department of Medical Physics Institute of Modern PhysicsChinese Academy of Sciences Key Laboratory of Heavy Ion Radiation Medicine Chinese Academy of Sciences Key Laboratory of Heavy Ion Radiation Medicine of Gansu Province University of Chinese Academy of Sciences Lanzhou Agricultural Science and Technology Research Promotion Center

This study is first to investigate proteomic changes in sheep sperm induced by carbon ion radiation using two-dimensional electrophoresis （2-DE） analysis in the project of breeding a new variety of sheep. Differential expression proteins were detected using the PDQuest 8.0 software after staining with Coomassie blue. Valid spots were then analyzed through liquid chromatography tandem mass spectrometry （LC-MS/MS）. Among the 480 total protein spots displayed in 2-D gels, 6 specific protein spots were observed in sperm gels. A search against protein sequences in the National Center for Biotechnology Information databases （NCBI） indicated that differentially expressed proteins correspond to two proteins, identified to be enolase and transcription factor AP-2-alpha （TFAP-2c0. The two proteins were up-regulated in the irradiated sperm. To the best of our knowledge, this study is the first to identify proteomic changes induced by carbon ion radiation in sheep sperm. The analysis of differential expression protein may be useful in identifying new breeding markers in sheep reproduction and in clarifying the mechanisms involved in irradiation or space breeding.

关键词： sheep sperm protein two-dimensional polyacrylamide gel electrophoresis analysis proteome carbon ionradiation irradiation breeding

A STAMP-Game model for accident analysis in oil and gas industry

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Petroleum Science 2024年第3期21卷 2154-2167页

作者： Huixing Meng xu An Daiwei Li Shijun zhao Enrico Zio xuan Liu Jinduo xing State Key Laboratory of Explosion Science and Safety Protection Beijing Institute of TechnologyBeijing100081China School of Emergency Management and Safety Engineering China University of Mining and Technology(Beijing)Beijing100083China Energy Department Politecnico di MilanoVia La Masa 34Milan20156Italy Mines Paris PSL Centre de Recherche sur les Risques et les CrisesSophie Antipolis06904France School of Mechanical-Electronic and Vehicle Engineering Beijing University of Civil Engineering and ArchitectureBeijing100044China

Accidents in engineered systems are usually generated by complex socio-technical *** is beneficial to investigate the increasing complexity and coupling of these factors from the perspective of system *** on system and control theories,System-Theoretic Accident Model and Processes(STAMP)is a widely recognized approach for accident *** this paper,we propose a STAMP-Game model to analyze accidents in oil and gas storage and transportation *** in accident analysis by STAMP can be regarded as players of a *** theory can,thus,be adopted in accident analysis to depict the competition and cooperation between ***,we established a game model to study the strategies of both supervisory and supervised *** obtained results demonstrate that the proposed game model allows for identifying the effectiveness deficiency of the supervisory entity,and the safety and protection altitudes of the supervised *** STAMP-Game model can generate quantitative parameters for supporting the behavior and strategy selections of the supervisory and supervised *** quantitative data obtained can be used to guide the safety improvement,to reduce the costs of safety regulation violation and accident risk.

关键词： Accident analysis STAMP System engineering Gametheory Oil and gas storage and transportation systems

Ionizing Radiation-Induced RPL23a Reduction Regulates Apoptosis via RPL11-MDM2-p53 Pathway in Mouse Spermatogonia

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Biomedical and Environmental Sciences 2021年第10期34卷 789-802页

作者： HE Yu xuan GUO Yi Xiao ZHANG Yong HU Jun Jie DONG Wei Tao DU Xiang Hong zhao xing xu College of Veterinary Medicine Gansu Agricultural UniversityLanzhou 730070GansuChina Department of Radiation Oncology Gansu Provincial HospitalLanzhou 730000GansuChina

Objective The expression patterns of ribosomal large subunit protein 23 a(RPL23 a)in mouse testes and GC-1 cells were analyzed to investigate the potential relationship between RPL23 a expression and spermatogonia apoptosis upon exposure to *** Male mice and GC-1 cells were irradiated with X-ray,terminal dUTP nick end-labelling(TUNEL)was performed to detect apoptotic spermatogonia in *** rate and cell cycle phase of GC-1 cells were analyzed with flow *** interactions were detected by Immunoprecipitation and protein localization as studied by *** and real-time PCR were applied to analyze to protein and gene *** Ionizing radiation(IR)increased spermatogonia apoptosis,the expression of RPL11,MDM2 and p53,and decreased RPL23 a expression in mice spermatogonia in vivo and in ***23 a knockdown weakened the interaction between RPL23 a and RPL11,leading to p53 ***,knockdown and IR decreased RPL23 a that induces spermatogonia apoptosis via RPL23 a-RPL11-MDM2-p53 pathway in GC-1 *** These results suggested that IR reduced RPL23 a expression,leading to weakened the RPL23 a-RPL11 interactions,which may have activated p53,resulting in spermatogonia *** results provide insights into environmental and clinical risks of radiotherapy following exposure to IR in male *** graphical abstract was available in the web of ***.

关键词： Ionizing radiation Testis Spermatogonia apoptosis

Dynamic plugging regulating strategy of pipeline robot based on reinforcement learning

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Petroleum Science 2024年第1期21卷 597-608页

作者： xing-Yuan Miao Hong zhao College of Mechanical and Transportation Engineering China University of PetroleumBeijing102249China

Pipeline isolation plugging robot (PIPR) is an important tool in pipeline maintenance operation. During the plugging process, the violent vibration will occur by the flow field, which can cause serious damage to the pipeline and PIPR. In this paper, we propose a dynamic regulating strategy to reduce the plugging-induced vibration by regulating the spoiler angle and plugging velocity. Firstly, the dynamic plugging simulation and experiment are performed to study the flow field changes during dynamic plugging. And the pressure difference is proposed to evaluate the degree of flow field vibration. Secondly, the mathematical models of pressure difference with plugging states and spoiler angles are established based on the extreme learning machine (ELM) optimized by improved sparrow search algorithm (ISSA). Finally, a modified Q-learning algorithm based on simulated annealing is applied to determine the optimal strategy for the spoiler angle and plugging velocity in real time. The results show that the proposed method can reduce the plugging-induced vibration by 19.9% and 32.7% on average, compared with single-regulating methods. This study can effectively ensure the stability of the plugging process.

关键词： Pipeline isolation plugging robot Plugging-induced vibration Dynamic regulating strategy Extreme learning machine Improved sparrow search algorithm Modified Q-learning algorithm