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A unique role of the pyrimidine de novo synthesis enzyme ODCase in Lysobacter enzymogenes

Journal of Integrative Agriculture 2024年第9期23卷 3066-3077页

作者： Mingming Yang Yunxiao Tan Jiabing Ma Yingjia zhao Xia Yan Nana Wang Pingping Wang jiaqi Tan Suilong Ai Xiaofei Liang Bangshuai Chang Obadah E.A.Yousif Chao zhao Bo Wang Guoliang Qian Lili Huang State Key Laboratory for Crop Stress Resistance and High-Efficiency Production Northwest A&F UniversityYangling 712100China College of Plant Protection Northwest A&F UniversityYangling 712100China College of Life Sciences Northwest A&F UniversityYangling 712100China College of Natural Resources and Environment Northwest A&F UniversityYangling 712100China Shaanxi Provincial Tobacco Corporation of China National Tobacco Corporation Xi’an 710061China Institute of Plant Protection Jiangsu Academy of Agricultural SciencesNanjing 210014China College of Plant Protection Laboratory of Plant ImmunityNanjing Agricultural UniversityNanjing 210095China

Bacterial species of the genus Lysobacter are environmentally ubiquitous with strong antifungal biocontrol ***-stable antifungal factor(HSAF)secreted by the biocontrol bacterium Lysobacter enzymogenes OH11 has broad-spectrum and highly efficient antifungal *** the biosynthetic regulations of HSAF would lay an important foundation for strain engineering toward improved HSAF *** this work,we demonstrate that Le0752,an orotidine-5´-phosphate decarboxylase enzyme(ODCase)catalyzing a pivotal step of the UMP de novo biosynthesis pathway,is vital for HSAF-mediated antimicrobial activities and growth of *** OH11,but not for twitching *** gene regulates the production of HSAF by affecting the expression of lafB,a key gene in the HSAF biosynthesis operon,through the transcription factor ***,bioinformatics analysis revealed that Le0752 belongs to the Group III ODCases,whereas its homologs in the closely related genera Xanthomonas and Stenotrophomonas belong to Group I,which contains most ODCases from Gram-positive bacteria,Gram-negative bacteria and ***,the Group I ODCase PXO_3614 from the Xanthomonas oryzae *** PXO99A strain complemented the Le0752 mutant in regulating HSAF-mediated antagonistic ***,these results highlight the important requirement of de novo pyrimidine biosynthetic enzymes for antibiotic HSAF production in ***,which lays an important foundation for improving HSAF production via metabolic flow design and for dissecting the regulatory functions of bacterial ODCases.

关键词： ODCase pyrimidine de novo synthesis secondary metabolite HSAF Lysobacter enzymogenes

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Femtosecond laser 3D printed micro objective lens for ultrathin fiber endoscope

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Fundamental Research 2024年第1期4卷 123-130页

作者： Bozhe Li Changrui Liao Zhihao Cai Jie Zhou Cong zhao Liqing Jing jiaqi Wang Cong Xiong Lei Xu Ying Wang Yiping Wang Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education/Guangdong Province College of Physics and Optoelectronic EngineeringShenzhen UniversityShenzhen 518060China Shenzhen Key Laboratory of Photonic Devices and Sensing Systems for Internet of Things Guangdong and Hong Kong Joint Research Centre for Optical Fibre SensorsShenzhen UniversityShenzhen 518060China School of Electronic and Communication Engineering Shenzhen Polytechnic UniversityShenzhen 518055China

The most important optical component in an optical fiber endoscope is its objective *** achieve a high imaging performance level,the development of an ultra-compact objective lens is thus the key to an ultra-thin optical fiber *** this work,we use femtosecond laser 3D printing to develop a series of micro objective lenses with different optical *** imaging resolution and field-of-view performances of these printed micro objective lenses are investigated via both simulations and *** the first time,multiple micro objective lenses with different fields of view are printed on the end face of a single imaging optical fiber,thus realizing the perfect integration of an optical fiber and objective *** work demonstrates the considerable potential of femtosecond laser 3D printing in the fabrication of micro-optical systems and provides a reliable solution for the development of an ultrathin fiber endoscope.

关键词： Femtosecond laser Two-photon polymerization 3D printing Micro objective lens Fiber endoscope

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Neural stem cell-derived exosomes regulate cell proliferation,migration,and cell death of brain microvascular endothelial cells via the miR-9/Hes1 axis under hypoxia

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Animal Models and Experimental Medicine 2024年第1期7卷 24-35页

作者： Xiaojun Deng Xiaoyi Hu Shang Wang Hui zhao Yaqin Wei jiaqi Fu Wenhui Wu Jinming Liu Caicai Zhang Lili Wang Ping Yuan Department of Critical Care Medicine Shanghai Sixth People's HospitalShanghai Jiao Tong UniversityShanghaiChina Department of Cardio-Pulmonary Circulation Shanghai Pulmonary HospitalSchool of MedicineTongji UniversityShanghaiChina Institute of Bismuth Science University of Shanghai for Science and TechnologyShanghaiChina Department of Physiology Hainan Medical University HaikouHainanChina Department of Clinical Medical Laboratory Center Hangzhou Red Cross HospitalZhejiangChina

Background:Our previous study found that mouse embryonic neural stem cell(NSC)-derived exosomes(EXOs)regulated NSC differentiation via the miR-9/Hes1 ***,the effects of EXOs on brain microvascular endothelial cell(BMEC)dysfunction via the miR-9/Hes1 axis remain ***,the current study aimed to determine the effects of EXOs on BMEC proliferation,migration,and death via the miR-9/Hes1 ***:Immunofluorescence,quantitative real-time polymerase chain reaction,cell counting kit-8 assay,wound healing assay,calcein-acetoxymethyl/propidium iodide staining,and hematoxylin and eosin staining were used to determine the role and mechanism of EXOs on ***:EXOs promoted BMEC proliferation and migration and reduced cell death under hypoxic *** overexpression of miR-9 promoted BMEC prolifera-tion and migration and reduced cell death under hypoxic ***,miR-9 downregulation inhibited BMEC proliferation and migration and also promoted cell ***1 silencing ameliorated the effect of amtagomiR-9 on BMEC proliferation and migration and cell *** structures were observed in the regions of the hippocampus and cortex in hypoxia-induced ***,EXO treatment improved cerebrovascular ***:NSC-derived EXOs can promote BMEC proliferation and migra-tion and reduce cell death via the miR-9/Hes1 axis under hypoxic ***,EXO therapeutic strategies could be considered for hypoxia-induced vascular injury.

关键词： brain microvascular endothelial cells exosomes Hes1 miR-9 neural stem cells

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软腭及咽部肌肉神经解剖研究进展

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中华整形外科杂志 2022年第11期38卷 1310-1314页

作者：赵佳琪吴镝中国医学科学院北京协和医学院整形外科医院整形一科北京100144

该文综述了软腭及咽部肌肉神经解剖相关的研究进展。软腭部肌肉分为腭帆张肌、腭帆提肌、腭咽肌、腭舌肌及悬雍垂肌。其中, 腭帆张肌受三叉神经的下颌神经分支支配;大多数学者认为其他软腭肌由迷走神经、舌咽神经形成的咽丛分支支配, ... 详细信息

该文综述了软腭及咽部肌肉神经解剖相关的研究进展。软腭部肌肉分为腭帆张肌、腭帆提肌、腭咽肌、腭舌肌及悬雍垂肌。其中, 腭帆张肌受三叉神经的下颌神经分支支配;大多数学者认为其他软腭肌由迷走神经、舌咽神经形成的咽丛分支支配, 面神经及腭小神经亦参与其中。咽肌可分为咽缩肌和咽提肌, 咽缩肌包括咽上缩肌、咽中缩肌及咽下缩肌;咽提肌包括茎突咽肌、腭咽肌和咽鼓管咽肌。咽上缩肌及咽中缩肌被认为由咽丛分支支配, 而咽下缩肌则由咽丛及喉部神经支配;茎突咽肌由舌咽神经分支支配, 而腭咽肌和咽鼓管咽肌则由咽丛分支支配。了解腭咽部肌肉的神经支配有助于减少腭裂修复术中的神经损伤, 有助于了解腭咽闭合不全所致的吞咽、发音等障碍的相关机制, 并指导神经源性吞咽困难及部分睡眠呼吸暂停综合征的治疗。

关键词：咽肌软腭肌解剖学神经支配

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Effects of Dietary Changes on the Gut Microbiota of Cynops orientalis

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Asian Herpetological Research 2024年第2期15卷 63-72页

作者： Yuting WANG Yuning XIE Siyu WU Wenwen ZHANG Xiner CHENG Zixuan LI Fangfei HAN Jiayi SHI Yingying SHI Zhirong HE Chunna ZHANG Yixin JIANG Na zhao jiaqi LI Supen WANG College of Life Sciences Anhui Normal UniversityWuhu 241000AnhuiChina Nanjing Institute of Environmental Sciences Ministry of Ecology and Environment of ChinaNanjing 210042JiangsuChina School of Ecology and Environment Anhui Normal UniversityWuhu 241002AnhuiChina Key Laboratory of Zoological Systematics and Evolution Institute of ZoologyChinese Academy of SciencesBeijing 100101China

The gut microbiota plays a pivotal role in the maintenance of health for amphibians,and it has been fully recognized,but the effectiveness of various influencing factors has not yet been fully *** this association should be considered while the amphibian order Caudata is facing a severe situation of population decline and extinction,there is little understanding of the association between diets and the diversity of gut microbiota in the amphibian order ***,we conducted an extensive analysis of the gut microbiota of Cynops orientalis fed different diets using functional prediction and 16S rRNA amplicon sequencing ***,we found that wild individuals had greater gut microbial diversity and richness in comparison to captive ***,we identified the bacterial taxa associated with diets and observed differences in the relative abundance of gut microbiota among people on various ***,we have a predictive comprehension of the selection and adaptative significance of shared core ASVs in the gut microbiota in maintaining the healthy survival of *** in a large-scale spatiotemporal *** study emphasizes how diets alter the gut microbiota of Caudata and offers fresh perspectives on the conservation and captive management of species in Caudata.

关键词： 16S rRNA Caudata core microbiota diet gut microbiota

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作为媒介的设计

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装饰 2023年第3期 12-16页

作者：胡飞赵佳琦同济大学设计创意学院

本文旨在重新探讨设计与媒介的关系。首先通过梳理媒介发展简史来理解媒介性,包括意义性、感知性和交互性,然后分别对设计之物、设计话语和设计思维的媒介性进行分析,再从媒介的三个特性和媒介的受众来审视设计。研究表明:设计之物、设... 详细信息

本文旨在重新探讨设计与媒介的关系。首先通过梳理媒介发展简史来理解媒介性,包括意义性、感知性和交互性,然后分别对设计之物、设计话语和设计思维的媒介性进行分析,再从媒介的三个特性和媒介的受众来审视设计。研究表明:设计之物、设计话语和设计思维都在不同程度上具有媒介性,设计可以也应该成为沟通设计界内、外部的媒介,为更多的学科和社会实践提供启发。

关键词：媒介性设计之物设计话语设计思维

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在荆棘丛中开辟道路——哈萨克族诗人阿拜的启蒙主义思想及其局限

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西域研究 2006年第1期 104-108页

作者：范学新赵嘉麒江苏苏州大学文学院新疆伊犁师范学院

阿拜的启蒙主义思想自成体系,以“破民族固陋”为起点,以“立民族品格”为主要目的,倡导民主与科学,追求民族新生。然而,受到主观与客观条件的制约,阿拜粗砾直率的呐喊,单一的思想启蒙,使他的启蒙道路不可避免地陷入孤独与失望之中,不... 详细信息

阿拜的启蒙主义思想自成体系,以“破民族固陋”为起点,以“立民族品格”为主要目的,倡导民主与科学,追求民族新生。然而,受到主观与客观条件的制约,阿拜粗砾直率的呐喊,单一的思想启蒙,使他的启蒙道路不可避免地陷入孤独与失望之中,不得不借助宗教来宣扬自己的科学主张,他的启蒙工作只能始于呐喊而终于彷徨。

关键词：启蒙主义批判精神民族品格阿拜哈萨克族诗歌

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光驱动C1转换到高附加值化学品的研究进展

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高等学校化学学报 2020年第4期41卷 604-615页

作者：李振华施润赵家琦张铁锐中国科学院理化技术研究所光化学转化与光电材料重点实验室北京100190 华中师范大学化学学院武汉430079

阐述了光驱动C1化学的最新研究进展,分别对光驱动费托合成、水煤气变换、二氧化碳加氢、甲烷重整和甲醇重整制氢的研究进行了综述,提出了当前研究存在的问题及发展方向.

关键词：光驱动催化 C1化学高附加值化学品费托合成二氧化碳加氢

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Direct and rapid detection of serum amino acid and monoamine neurotransmitters to assist the diagnosis of panic disorder

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Science China Chemistry 2024年

作者： Shiwen Liu Xuerui Wan Meng zhao jiaqi Wang Weilan Wu Linlin You Yonggui Yuan Qian Xu Rong Gao Key Laboratory of Environmental Medicine Engineering Ministry of Education School of Public Health Southeast University Key Laboratory of Modern Toxicology of Ministry of Education Department of Hygienic Analysis and Detection School of Public HealthNanjing Medical University Department of Psychosomatics and Psychiatry Zhongda Hospital School of Medicine Southeast University

The measurement of amino acid and monoamine neurotransmitters（NTs） in serum plays a central role in the identification and monitoring of clinical panic disorder（PD）. However, the strong polarity and chemical heterogeneity of NTs make direct extraction and rapid detection extremely challenging. Herein, we developed a strategy that integrated solid phase extraction based on magnetic Fe3O4coated with polydopamine（Fe3O4@PDA） and direct analysis of mass spectrometry in real time（DART-MS） for quantification of 10 NTs. We systematically investigated the optimal conditions of this strategy to achieve good accuracy and precision. Compared with existing magnetic solid phase extraction methods for NTs detection in biological samples, this strategy could adsorb more targets while having similar adsorption efficiencies, and the total process time was reduced by an average of 69.42%. We rapidly determined the metabolic abnormality of 6 types of NTs in the serum of PD patients and demonstrated their diagnostic accuracy. In addition, an outstanding feature of PD in which tryptophan was more metabolized by the KYN pathway and the 5-hydroxytryptamine pathway was inhibited could be elucidated by this strategy. Our results highlighted the potential ability of Fe3O4@PDA coupled with DART-MS to assist in the clinical diagnosis of PD.

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基于有限长线接触的直齿轮弹流润滑数值模拟

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东南大学学报（自然科学版） 2015年第4期45卷 696-700页

作者：欧阳天成陈南赵家琦王攀东南大学机械工程学院南京211189 广西大学土木工程学院南宁530004

针对具有旋转与轴向2个方向速度的胶印机窜墨棍直齿轮润滑问题,提出一种基于有限长线接触的直齿轮弹性流体动压润滑的数值模拟方法.首先,对模型进行几何分析,并运用有限长弹流理论建立了包含2个方向速度的控制方程,如Reynolds方程、油... 详细信息

针对具有旋转与轴向2个方向速度的胶印机窜墨棍直齿轮润滑问题,提出一种基于有限长线接触的直齿轮弹性流体动压润滑的数值模拟方法.首先,对模型进行几何分析,并运用有限长弹流理论建立了包含2个方向速度的控制方程,如Reynolds方程、油膜几何方程、润滑油黏度、密度方程和载荷平衡方程;然后,对控制方程进行无量纲化以提高数值计算效率和求解的收敛性;最后,采用有限差分法对具有强非线性的控制方程进行离散化,并采用多重网格法进行数值求解.数值计算结果表明,该方法能有效获得直齿轮不同工况下的润滑油膜压力、膜厚分布,同时还可得到相应的油膜摩擦系数,为研究具有2个方向速度的直齿轮摩擦问题提供了一种有效途径.

关键词：二维速度有限长线接触直齿轮弹流润滑多重网格法

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