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ZF-RNase-5,a zebrafish ortholog of angiogenin,regulates yolk extension through a p53-dependent apoptosis pathway

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ZF-RNase-5,a zebrafish ortholog of angiogenin,regulates yolk...

华东六省一市生物化学与分子生物学学会2014年学术交流大会暨浙江省第十一届学会会员代表大会

作者： Yixing Zhai Xinyuan zhao Jinghao Sheng guangdi Chen zhao Ou Zhengping Xu Research Center of Molecular Medicine Zhejiang University School of Medicine Key Laboratory for Molecular Animal Nutrition Ministry of EducationZhejiang University College of Animal Sciences

Recently,five zebrafish ortholog of human angiogenin(hANG)genes,which known as ZF-RNase-1/2/3/4/5 respectively,have been identified and *** these five members,the ZF-RNase-5 is the most similar to hANG in three-dimensional structure,thermodynamic behavior and biological *** ZF-RNase-5 is strongly expressed during the whole embryonic/larval development,the unique functions of ZF-RNase-5 in zebrafish development remain to be ***,we investigated the temporal and spatial expression patterns of ZF-RNase-5 at all stage of development by real-time quantitative PCR and in situ *** with previous research,ZF-RNase-5 expression level continues to increase throughout the whole development *** situ hybridization analyses revealed that ZF-RNase-5 widely expressed in the whole embryos before 8 hours post fertilization(hpf),then displayed significantly tissue specific expression pattern which were located in the anterior-posterior embryonic axis close to the ventral of the trunk,yolk syncytial layer(YSL)cells around the yolk ball and yolk extension,and primary head and around primary neural tube from 16 hpf to 24 *** examine the functions ofZF-RNase-5 on early developmental embryos,we knocked down the ZF-RNase-5 translation using two specific morpholino ***-RNase-5morphants exhibited severe yolk extension defects,which are observed since the last stage of yolk extension formation from 21 hpf to 24 ***-injection of ZF-RNase-5 mRNA with morpholino partially rescued the morphological ***,ZF-RNase-5-deficientembryos hardly hatch out and survive until 4-5 days post *** assay suggested that the abnormalities of ZF-RNase-5 morphants could be due to cell apoptosis in YSL cells that results in defective of yolk extension ***,apoptosis-related p53 pathway is significantly activated in ZF-RNase-5 *** data showed that the p53 target genes p21 and bax w

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平滑ARA~*算法在智能车辆路径规划的应用

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机械科学与技术 2017年第8期36卷 1272-1275页

作者：赵鑫胡广地西南交通大学机械工程学院成都630031

A~*算法是一种经典的启发式搜索算法,广泛应用于智能车辆的路径规划问题。但A~*算法效率低,不具有实时性。针对A~*算法的缺点,改进得到一种高效、实时的路径搜索算法ARA~*,ARA~*算法首先在一个松弛的约束条件下快速搜索到一条次优路径;... 详细信息

A~*算法是一种经典的启发式搜索算法,广泛应用于智能车辆的路径规划问题。但A~*算法效率低,不具有实时性。针对A~*算法的缺点,改进得到一种高效、实时的路径搜索算法ARA~*,ARA~*算法首先在一个松弛的约束条件下快速搜索到一条次优路径;然后在规划时间内逐渐加强约束条件,利用已搜索过的节点信息连续改进次优解,直到找到最优解或规划时间结束。其次,针对ARA~*算法得到的路径存在折线多、转折次数多等问题,对ARA~*算法得到的路径进行基于关键点的平滑处理。给出了平滑ARA~*算法流程,分析对比了各自的特点,通过栅格地图路径规划的MATLAB仿真结果验证了理论分析,同时仿真结果也说明平滑ARA~*算法的高效性、实时性。

关键词：路径规划 A^*算法平滑ARA^*算法

The effects of RF-EMF on genome stability of nervous system

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The effects of RF-EMF on genome stability of nervous system

华东六省一市生物化学与分子生物学学会2014年学术交流大会暨浙江省第十一届学会会员代表大会

作者： Liling Su Xinyuan zhao Chuan Sun Zhengping Xu guangdi Chen Bioelectromagnetics Laboratory Zhejiang University School of Medicine

Currently,there are about 7 billion mobile subscriptions around the world which accounts for96%of the population,and the people are exposed to the Radio Frequency Electromagnetic Fields(RF-EMF)emited by the mobile phone day and ***,public concerns are dramatically raised during the past *** on the epidemiological finding about the association between mobile phone use and brain tumors,the International Agency for Research on Cancer(IARC)has classified RF-EMF as human 2B ***,the data available from laboratory studies neither provide consistent corroborating evidence nor suggest mechanisms of action to support or clarify this epidemiological *** propose to investigate the weak biological effects induced by low-intensity of RF-EMF exposure using systemic strategies with more sensitive *** this study,we adopted H2AX foci formation,the most sensitive method currently available for detecting DNA double stand breaks(DSB)in cells,to systematically observe the effect of GSM 1800 MHz RF-EMF at SAR of 4.0 W/kg on the main experimental cell models of nervous system,including neuroblastoma cells(SH-SY5Y),human glioma cells(A172 and U251),primary cerebral cortex neurons,and microglia *** exposed for 1 h,6h or 24 h,the cells were subjected toγH2AX foci formation *** data analyses revealed there were no significant differences between the sham and RF-EMF exposure groups in all the examined cells,suggesting the RF-EMF exposure under current condition does not induce significant DNA DSB damage.

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跨临界CO_(2)两相流可调式喷射器的流动模拟和性能分析

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化工学报 2023年第11期74卷 4515-4526页

作者：陈壮刘光弟曾宏轩赵红霞山东大学能源与动力工程学院山东济南250061 西安交通大学能源与动力工程学院陕西西安710049

作为CO_(2)制冷/热泵系统重要的节能部件,喷射器的工作能力对系统性能的提升有着重要影响。尽管CO_(2)喷射器的结构简单,但其内部流动十分复杂,包含非平衡相变、跨声速和马赫波等物理现象。为此,本研究建立了用于CO_(2)两相流模拟的均... 详细信息

作为CO_(2)制冷/热泵系统重要的节能部件,喷射器的工作能力对系统性能的提升有着重要影响。尽管CO_(2)喷射器的结构简单,但其内部流动十分复杂,包含非平衡相变、跨声速和马赫波等物理现象。为此,本研究建立了用于CO_(2)两相流模拟的均相弛豫模型(HRM),通过实验数据验证了模型的可靠性,并以此开展了对可调式喷射器的数值研究,分析了有无探针、探针位置和工况变化对可调式喷射器性能和内部流动的影响。研究结果表明,探针增加了一次流的不可逆损失,同时也改变了喷嘴出口一次流的膨胀状态;通过探针调整喷嘴喉部面积,提高了喷射器适应不同工况运行的能力,使喷射器在不同工况下保持最佳的运行状态;与固定式喷射器相比,可调式喷射器效率平均提升10.74%。

关键词：可调式喷射器 CO_(2) 均相弛豫模型两相流计算流体力学

The effects of 50 Hz magnetic fields on DNA damage in human lens epithelial cells

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The effects of 50 Hz magnetic fields on DNA damage in human ...

华东六省一市生物化学与分子生物学学会2014年学术交流大会暨浙江省第十一届学会会员代表大会

作者： Xinyuan zhao Liling Su Chuan Sun Yan Zhang guangdi Chen Zhengping Xu Bioelectromagnetics Laboratory Zhejiang University School of Medicine

With the development of electric power industry and widely using of electronic products,public concerns have been raised about possible health effects of occupational and environmental exposure to extremely low frequency magnetic fields(ELF MF).Epidemiological studies have suggested that exposure to environmental ELF MF may be associated with increased risks of different diseases,such as cancer,Alzheimer’s diseaseand adverse birth outcome.A large number of laboratory studies,including in vivo and in vitro studies,have been conducted to investigate the biological effects induced by low intensity ELF MF exposure and the underlying *** them,the effects of ELF MF exposure on cellular genotoxicity have been widely ***,despite of studies for many years,the results on the genotoxicity of ELF MF exposure are *** investigate the effect of electromagnetic fields(EMF)on genome stability in human lens epithelial cells,the cells were exposed to 50 Hz sinusoidal MF at 1.0mT or 2.0 mT for 1 h,6 h,and 24 ***γ-H2AX foci formation assay was used to detect DNA double-strand breaks in the *** alkaline comet assay was performed to detect DNA *** to the control group,exposure to 50 Hz MF at 1.0 mT did not significantly increase the average number ofγ-H2AX foci per cell and the percentage of positive cells under any of exposure ***,exposure to 50 Hz MF at 2.0 mT for 1 h,6 h or 24 h did not significantly increase theγ-H2AX foci *** results suggest that exposure to 50 Hz MF up to 2.0 mT did not induce DNA double-strandbreaks in *** alkaline comet assay analyses showed that the MF exposure at 1.0 mT or 2.0mT for 24 h did not significantly increase DNA fragments when comparing withcontrol *** conclusion,our data suggest that 50 Hz MF exposure does not induce DNA damage in human lens epithelial cells under current exposure conditions.

关键词：

50 Hz magnetic fields induces G2/M cell cycle arrest in human choriocarcinoma cell line JAR cells

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50 Hz magnetic fields induces G2/M cell cycle arrest in huma...

华东六省一市生物化学与分子生物学学会2014年学术交流大会暨浙江省第十一届学会会员代表大会

作者： Chuan Sun Xiaoxia Wei Liling Su Xinyuan zhao guangdi Chen Zhengping Xu Bioelectromagnetics Laboratory Zhejiang University School of Medicine

The intensity of environmental electromagnetic fields(EMF)is increasing fast,and public concerns have been raised about the possible health hazards related to the exposure of *** epidemiological studies show that exposure to extremely low frequency magnetic fields(ELF-MF)is associated with adverse effects on *** vivostudies showalso indicate that ELF-MF exposure affects reproductive ability in *** DNA integrity is crucial for the cellular function of trophoblast cells,which maintain the normal reproductive ***,it remains unclear whether ELF-MF exposure influence the DNA integrity of trophoblast *** the present work,the humanchoriocarcinoma JAR cells were exposed for 24 h to 50 Hz MF at 2.0 *** alkalinecomet assay was used to evaluate DNA damages(e.g.,DNA strand breaks,DNA fragments).The cell cycle progression was evaluated by flow cytometry *** a result,the 50 Hz MF exposure for 24 h significantly increased the DNA fragments in JAR cells,and arrested the cells at G2/M *** determine which type of DNA damage was induced by 50 Hz MF exposure,the neutral comet assay and yH2AX(the phosphorylation form of histone H2AX)foci formation assay were applied to detect DNA double strand breaks(DSBs)in JAR *** assays revealed that 50 Hz MF exposure did not *** results suggest that 50 Hz MF exposure increases DNA single strand breaks(SSBs).In conclusion,our data indicate that 50 Hz MF exposure induce DNA single but not double strand ***,whether the 50 Hz MF exposure-induced G2/M cell cycle arrest in JAR cells is caused by SSBs await further studies.

关键词：

键合技术在微机械Golay-cell红外探测器中的应用

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仪器仪表学报 2006年第8期27卷 936-939页

作者：赵林林徐晨赵慧霍文晓沈光地北京工业大学电子信息与控制工程学院北京市光电子技术实验室北京100022

介绍多种硅片键合技术及其在基于高莱盒(Golay-cell)原理的微机械红外探测器中的应用。对多种键合方法在该器件中的实验结果进行比较,确定了现阶段最优的键合方法,即采用局部电场屏蔽方法的阳极键合方法,键合成功率在90%以上,并初步实... 详细信息

介绍多种硅片键合技术及其在基于高莱盒(Golay-cell)原理的微机械红外探测器中的应用。对多种键合方法在该器件中的实验结果进行比较,确定了现阶段最优的键合方法,即采用局部电场屏蔽方法的阳极键合方法,键合成功率在90%以上,并初步实现了器件的标准化制作。

关键词：微电子机械系统高菜盒红外探测器键合

Fluorescent AIE dots encapsulated organically modified silica (ORMOSIL) nanoparticles for two-photon cellular imaging

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中国科学：化学 2013年第9期43卷 1214-1214页

作者： ZHU ZhenFeng zhao XinYuan QIN Wei CHEN guangdi QIAN Jun XU ZhengPing Centre for Optical and Electromagnetic Research Zhejiang Provincial Key Laboratory for Sensing Technologies Zhejiang University Bioelectromagnetics Laboratory School of Medicine Zhejiang University Department of Chemistry The Hong Kong University of Science and Technology

2,3-Bis(4-(phenyl(4-(1,2,2-triphenylvinyl)phenyl)amino)phenyl)fumaronitrile(TPE-TPA-FN or TTF),which possesses aggregation-induced emission(AIE)characteristic,is doped in organically modified silica(ORMOSIL)*** increasing the weight ratio of TTF to the precursor of silica nanoparticles(the quantities of the precursors were kept the same),the fluorescence intensity of nanoparticles increased correspondingly,due to the formation of larger AIE dots in the cores of ORMOSIL *** fluorescent and biocompatible nanoprobes were then utilized for in vitro imaging of HeLa ***-photon fluorescence microscopy clearly illustrated that the nanoparticles have the capacity of nucleus permeability,as well as cytoplasm staining towards tumor *** experimental results may offer a promising method for fast and bright fluorescence imaging,as well as bio-molecule/drug delivery to cell nucleus.

关键词：二氧化硅纳米粒子荧光强度细胞成像有机改性双光子硅纳米颗粒封装 HeLa细胞

A hormesis-like effect of 1800 MHz radio frequency electromagnetic fields on DNA damage in mouse embryonic fibroblasts

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A hormesis-like effect of 1800 MHz radio frequency electroma...

中国生物化学与分子生物学会2016年全国学术会议

作者： Chuan Sun Xiaoxia Wei Yue Fei Liling Su Xinyuan zhao guangdi Chen Zhengping Xu Bioelectromagnetics Laboratory Zhejiang University School of Medicine Institute of Environmental Health Zhejiang University School of Medicine Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases Zhejiang University

Radiofrequency electromagnetic fields(RF-EMFs)have been classified by the International Agency for Research on Cancer as possible carcinogens for humans;however,this conclusion is based on limited epidemiological findings and lacks solid support from experimental *** particular,there are no consistent data regarding the genotoxicity of ***

关键词： RF-EMFs Mouse embryonic fibroblasts ATM hormesis-like effect