The effects of low intensity of electromagnetic fields on DNA damage
作者单位：Bioelectromagnetics LaboratoryZhejiang University School of Medicine
会议名称：《第八届全国医学生物化学与分子生物学第五届全国临床应用生物化学与分子生物学2013华东六省一市生物化学与分子生物学联合学术研讨会》
会议日期：2013年
学科分类：100405[医学-卫生毒理学] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 10[医学]
基 金：supported by grants from the Ministry of Science and Technology(2011CB503702) the Natural Science Foundation of China(81102094) the Research Fund for the Doctoral Program of Higher Education of China(20110101120100)
关 键 词：Electromagnetic field DNA damage mouse embryonic fibroblasts ATM deficient γ-H2AX
摘 要：With the rapid development of electric power and wireless communication technologies over the last several decades,public concerns have been raised about possible health effects(*** cancer risk) of exposure to environmental electromagnetic fields(EMF),including extremely low frequency magnetic fields(ELF-MP) emitted from power lines and radiofrequency electromagnetic fields(RF-EMF) related with mobile *** recent study showed that RF-EMF exposure induced γH2 AX foci formation,a sensitive indicator of DNA double strand breaks(DSBs),in human skin *** this study,we have examined the effect of ELF-MF or RF-EMF exposure on DNA damage in cells from nervous *** results showed that exposure to 1800 MHz RF-EMF at SAR of 4 W/kg or 50 Hz MF at 2 mT or 1 mT for 1 h,6 h,and24 h did not induce statistically significant changes ofγH2 AX foci formation in microglia cells and U251 *** addition,we used the ataxia telangiectasia mutated gene deficient(ATM)and wide type(ATM) mouse embryonic fibroblasts(MEFs) as cell models,and found that exposure to MF under the current experimental condition did not change the γ-H2 AX foci formation in ATM MEF;however,in ATM MEF,comparing to the sham-exposure group,exposure to 2.0 mT but not 0.4 mT or 1.0 mT MF for 1 h significantly decreased the percentage of positive cells(p0.01).Our data suggest that the effects of EMF on DNA damage may depend on the cell type or the cellular genetic *** biological consequences of decreased γ-H2 AX foci formation in ATM MEF responding to 2.0 mT MF exposure await further investigations.