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Hormonal Regulation of Leaf Morphogenesis in Arabidopsis

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Journal of Integrative Plant Biology 2007年第1期49卷 75-80页

作者： Lin-Chuan Li Ding-Ming Kang zhang-liang chen Li-Jia Qu National Laboratory for Protein Engineering and Plant Genetic Engineering Peking-Yale Joint Research Center for Plant Molecular Genetics and AgroBiotechnology College of Life Sciences Peking University Beijing 100871 China Key Laboratory of Crop Genomics and Genetic Improvement of Ministry of Agriculture/Key laboratory of Crop Genetic Improvement of Beijing City College of Agronomy and Biotechnology China Agricultural University Beijing 100094 China

Leaf morphogenesis is strictly controlled not only by intrinsic genetic factors, such as transcriptional factors, but also by environmental cues, such as light, water and pathogens. Nevertheless, the molecular mechanism of how leaf morphogenesis is regulated by genetic programs and environmental cues is far from clear. Numerous series of events demonstrate that plant hormones, mostly small and simple molecules, play crucial roles in plant growth and development, and in responses of plants to environmental cues such as light. With more and more genetics and molecular evidence obtained from the model plant Arabidopsis, several fundamental aspects of leaf morphogenesis including the initiation of leaf primordia, the determination of leaf axes, the regulation of cell division and expansion in leaves have been gradually unveiled. Among these phytohormones, auxin is found to be essential in the regulation of leaf morphogenesis.

关键词： auxin curly leaf hormonal regulation leaf morphogenesis leaf shape

Key technologies for construction of Jinping traffic tunnel with an extremely deep overburden and a high water pressure

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Journal of Modern Transportation 2011年第2期19卷 94-103页

作者： Shougen chen Heng zhang Xinrong TAN liang chen School of Civil Engineering Southwest Jiaotong University Chengdu 610031 China

Jinping traffic tunnel is one of the deepest traffic tunnels in the world with a maximum overburden of 2 375 m and the overburden over 73% of its total length is larger than 1 500 m. The tunnel is 17.5 km long and designed to provide a shortcut road between two hydropower stations： Jinping I and Jinping II of the Jinping Hydropower Project, located on Yalong River, liangshan State, Sichuan Province, China. The tunnel is so deep that building any shafts is impossible. The construction starts from both ends （east and west ends）, and the construction length from the west end is 10 km with a blind heading. This paper deals with an overview of this project and analysis of the engineering features, as well as key technologies developed and applied during the construction, including geological prediction, rock burst prevention under a super high in-situ stress, sealing of groundwater with a high pressure and big flow rate, ventilation for a blind heading of 10 km, wet spraying of shotcrete at zones of rock burst and rich water, etc. The application of the new technologies to the construction achieved a high quality tunnel within the contract period.

关键词： key technologies Jinping traffic tunnel extremely deep overburden high water pressure

The Arabidopsis Anaphase-Promoting Complex/Cyclosome Subunit 1 is Critical for Both Female Gametogenesis and Embryogenesis

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Journal of Integrative Plant Biology 2013年第1期55卷 64-74页

作者： Yanbing Wang Yingnan Hou Hongya Gu Dingming Kang zhang-liang chen Jingjing Liu Li-Jia Qu State Key Laboratory of Protein and Plant Gene Research College of Life Sciences Peking University College of Agronomy and Biotechnology China Agricultural University The National Plant Gene Research Center

Anaphase-promoting complex/cyclosome （APC/C）, a multisubunit E3 ligase, plays a critical role in cell cycle control, but the functional characterization of each subunit has not yet been completed. To investigate the function of APC1 in Arabidopsis, we analyzed four mutant alleles of APC1, and found that mutation in APC1 resulted in significantly reduced plant fertility, accumulation of cyclin B, and disrupted auxin distribution in embryos. The three mutant alleles apcl-1, apcl-2 and apcl-3 shared variable defects in female gametogenesis including degradation, abnormal nuclear number, and disrupted polarity of nuclei in the embryo sac as well as in embryogenesis, in which embryos were arrested at multiple stages. All of these defects are similar to those previously identified in apc4. The mutant apcl-4, in which the T-DNA was inserted after the transmembrane domain at the C-terminus, showed much more severe phenotypes; that is, most of the ovules were arrested at the one-nucleate female gametophyte stage （stage FG1）. In the apcl apc4 double mutants, the fertility was further reduced by one-third in apcl-ll＋ apc4-1/＋, and in some cases no ovules even survived in siliques of apcl-4/＋ apc4-1/＋. Our data thus suggest that APC1, an essential component of APC/C, plays a synergistic role with APC4 both in female gametogenesis and in embryogenesis.

关键词： Arabidopsis anaphase-promoting complex/cyclosome (APC/C) APC1 APC4 female gametogenesis embryogenesis synergistic role

Keyhole approach surgery for petroclival meningioma

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Chinese Medical Journal 2006年第16期119卷 1339-1342页

作者： ZHU Wei MAO Ying ZHOU liang-fu zhang Rong chen liang Department of Neurosurgery Shanghai Neurosurgical Center Huashan Hospital of Fudan University Shanghai 200040 China

Background In China, the feasibility of keyhole approach in surgical treatment of petroclival meningioma has not been well evaluated. This report summarized our experience in 25 patients with petroclival meningioma who had been treated with keyhole approach surgery. Methods From July 2000 to July 2005, 25 patients with petroclival meningioma were subjected to resection via subtemporal, retrosigmoid or combined keyhole approaches. The extent of tumor resection was evaluated by MRI 3 months after surgery, and postoperative complications were investigated. Results The maximum diameter of tumors ranged from 2 to 7 cm （mean, 4.5 cm）. Gross total resection （GTR） was achieved in 14 patients, giving a GTR rate of 56%. Subtotal resection （STR） was carried out in 8 patients and partial resection in 3. Thirteen patients kept normal neurological status, whereas others suffered from cranial nerve deficits （Ⅶ, Ⅶ, Ⅲ and lower CN）. One patient died in the postoperative period. Conclusions Keyhole approach surgery, especially the combined keyhole approach is suitable for the treatment of petroclival meningioma. It provides easy and quick access to the supra- and infratentorial juxta-clival region without drilling of the petrous bone. Complications related to the approach can be minimized.

关键词： keyhole surgery petroclival meningioma subtemporal approach retrosigmoid approach

基于简便约束粒子群优化算法的空空导弹μ-PID控制器设计

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兵工学报 2017年第1期38卷 89-96页

作者：张民陈亮陈欣南京航空航天大学自动化学院江苏南京211106

针对现有约束粒子群优化(PSO)算法存在的算法复杂、应用范围受限、优化效果不佳等缺陷,提出一种新型约束粒子群算法。该算法采用目标函数替换的方法将约束优化问题转化为非约束优化问题,具有简便易用的优点。通过典型测试函数测试并和... 详细信息

针对现有约束粒子群优化(PSO)算法存在的算法复杂、应用范围受限、优化效果不佳等缺陷,提出一种新型约束粒子群算法。该算法采用目标函数替换的方法将约束优化问题转化为非约束优化问题,具有简便易用的优点。通过典型测试函数测试并和其他具有代表性的约束PSO算法进行对比,表明该算法在处理约束优化问题上的优越性。为了验证该算法应用于工程的可行性,以样例导弹纵向模型为对象,针对经典Raytheon控制结构,采用该算法设计了μ-PID控制器。仿真结果表明,样例导弹控制器可以在满足多种时域指标的同时具有良好的鲁棒性能,达到了设计指标要求,验证了所提出算法的有效性。

关键词：飞行器控制、导航技术空空导弹粒子群优化约束优化鲁棒性

Discovery of ultrahigh-pressure magnesite-bearing garnet lherzolite (>3.8 GPa) in the Altyn Tagh, Northwest China

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Chinese Science Bulletin 2002年第11期47卷 881-886页

作者： LIU liang, SUN Yong, XIAO Peixi, CHE Zicheng, LUO Jinhai, chen Danling, WANG Yan, zhang Anda, chen liang & WANG YongheKey Laboratory of Continental Dynamics of the Ministry of Education, Northwest University, Xi’an 710069, China The Northwest Institute of Key Laboratory of Continental Dynamics of the Ministry of Education Northwest University Xi’an China The Northwest Institute of Geology and Mineral Resources Xi’an China

Magnesite-bearing garnet Iherzolite from the Altyn Tagh, associated with garnet pyroxenite, and garnet-bearing felsic gneiss, crops out as lenses in Proterozoic gneiss about 100 km east of the Jianggelasayi River. The garnet Iherzolite, together with the eclogite in western Jianggelasayi, composes a high-pressure to ultrahigh-pressure metamorphic belt in the southern margin of the Altyn Tagh. Parageneses of minerals from magnesite-bearing garnet Iherzolite indicate that the rock evolved a multi-stage metamorphism. The peak-stage metamorphism produces an assemblage of Grt+Ol+Opx+Cpx±Mgs, in which A12O3 content of Opxis very low (0.30-0.66wt%). The calculated P-T condition of the peak stage is *** and 880-970°C, some exsolution rods of clinopyroxene and rutile occur in the Grt and magnesite is rimed by dolomite and orthopyroxene, all implying that the peak stage was a UHP metamorphic process. Together with regional geological studies, isotopic dating and the discovery of coesite inclusions in

关键词： garnet lherzolite magnesite UHP metamorphism Altyn Tagh Mountains.

Assessment of coastal vulnerability to environmental change in Jiangsu coastal plain

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Journal of Geographical Sciences 2001年第1期11卷 24-33页

作者： YANG Gui-shan, SHI Ya-feng, zhang chen, liang Hai-tang (Nanjing Institute of Geography and Limnology, CAS, Nanjing 210008, China) CAS Nanjing Institute of Geography and Limnology Nanjing China (GRID:grid.9227.e) (ISNI:***)

Assessment of coastal vulnerability to future environmental change has been emphasized in coastal nations or regions. The Jiangsu coastal plain, located to the north of the Yangtze River Delta in China, is most vulnerable to sea level rise and exacerbating coastal hazards. This paper develops the method of delimiting vulnerable scope and assessing coastal vulnerability through field observations and sampling and by applying remote sensing and GIS, which are suitable for great river delta and coastal plains with large area, relative complex micro-geomorphology and the protection of seawall. Applying this method, the coastal vulnerability of the Jiangsu coastal plain to relative sea level rise (approximately 50 cm up to the year 2050) and exacerbating storm surges have been assessed. The results show that, up to the year 2050, the Jiangsu coastal plain will probably lose 12.8 % of tidal flats (about 5.8x104 hm2) and 7.9 % of cultivated land (about 7.2x104 hm2). Meanwhile, 2.0 % of population, 3.8 % of original value of fixed assets, 3.2% of GDP (Gross Domestic Product), 40.3 % of salt industry and 5.8 % of aquiculture respectively will be affected due to coastal environmental change.

关键词： coastal vulnerability assessment vulnerable scope coastal environmental change Jiangsu coastal plain

Transgene inheritance and quality improvement by expressing novel HMW glutenin subunit (HMW-GS) genes in winter wheat

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Chinese Science Bulletin 2003年第8期48卷 771-776页

作者： zhang Xiaodong, liang Rongqi, chen Xqing, YANG Fengping & zhang Liquan Beijing Agro-Biotechnology Research Center, Beijing Academy of Ag-ricultural & Forestry Science, Beijing 100089, China Correspondence should be addressed to zhang Xiaodong (e-mail: zhangxd@***) Beijing Agro-Biotechnology Research Center Beijing Academy of Agricultural & Forestry Science China

The expression vector pBPC30, which carries the high molecular weight glutenin subunit (HMW-GS) 1Dx5 and 1Dy10 genes, was transferred into hexaploid winter wheat cv. Jinghua No. 1, Jing411 and Jingdong No. 6 ex-plants of immature embryos and immature inflorescence by particle bombardment. A large number of resistant trans-genic plants were obtained under the selection of herbicide bialaphos or phosphinothricin (PPT). Confirmed transgenic plants of T0 generation showed successful integration of HMW-GS genes and bar gene into the wheat genome. T1 generation of transgenic plants can resist 20150 mg/L PPT. Protein analysis of T2 seed by SDS-PAGE showed that HMW-GS 1Dx5 and 1Dy10 genes were well expressed in offspring seed of transgenic lines by co-expression with or substitution of endogenous 1Dx2 or 1Dy10. In one transgenic line, TG3-74, a new protein band between endogenous pro-tein subunits 7 and 8 (marked as 8*) of glutenin appeared, but endogenous subunit 8 (encoded by 1By8 gene) was ab-sent. Analysis of gluten rheological quality on seed proteins of 102 T3 plants showed that the sedimentation value of 5 transgenic lines (44.249.0 mL) was remarkably improved, 59.6%64.3% higher than that of wild type Jinghua No. 1 and Jingdong No. 6, similar to bread wheat Cheyenne (48.0 mL). Analysis of dough rheological properties of transgenic lines showed that the dough stable time of 5 transgenic lines range from 16 to 30 min, whereas the dough stable time of wild type was only between 37 min. Our research suggests that introducing novel HMW-GS genes into wheat is an effi-cient way to improve its bread-making quality.

关键词：转基因技术麦谷蛋白冬小麦基因表达

Magneto‑Dielectric Synergy and Multiscale Hierarchical Structure Design Enable Flexible Multipurpose Microwave Absorption and Infrared Stealth Compatibility

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Nano-Micro Letters 2025年第2期17卷 401-416页

作者： chen Li Leilei liang Baoshan zhang Yi Yang Guangbin Ji School of Electronic Science and Engineering Nanjing UniversityNanjing 210093People’s Republic of China College of Materials Science and Technology Nanjing University of Aeronautics and AstronauticsNanjing 210016People’s Republic of China

Developing advanced stealth devices to cope with radar-infrared(IR)fusion detection and diverse application scenarios is increasingly demanded,which faces significant challenges due to conflicting microwave and IR cloaking mechanisms and functional integration ***,we propose a multiscale hierarchical structure design,integrating wrinkled MXene IR shielding layer and flexible Fe_(3)O_(4)@C/PDMS microwave absorption *** top wrinkled MXene layer induces the intensive diffuse reflection effect,shielding IR radiation signals while allowing microwave to pass ***,the permeable microwaves are assimilated into the bottom Fe_(3)O_(4)@C/PDMS layer via strong magneto-electric *** theoretical and experimental optimization,the assembled stealth devices realize a near-perfect stealth capability in both X-band(8–12 GHz)and long-wave infrared(8–14μm)wavelength ***,it delivers a radar cross-section reduction of−20 dB m^(2),a large apparent temperature modulation range(ΔT=70℃),and a low average IR emissivity of ***,the optimal device demonstrates exceptional curved surface conformability,self-cleaning capability(contact angle≈129°),and abrasion resistance(recovery time≈5 s).This design strategy promotes the development of multispectral stealth technology and reinforces its applicability and durability in complex and hostile environments.

关键词： Microwave absorption Radar-infrared compatible stealth Wrinkled MXene Magneto-dielectric synergy Multifunction