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Acta Pharmaceutica Sinica B 2020年第11期10卷 2246-2257页

作者： Junjing Yin Haiqiang Cao Hong Wang Kaoxiang Sun Yaping Li zhiwen zhang School of Pharmacy Yantai UniversityYantai 264005China State Key Laboratory of Drug Research&Center of Pharmaceutics Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China Yantai Key Laboratory of Nanomedicine&Advanced Preparations Yantai Institute of Materia MedicaYantai 264000China

Hypoxia is a serious impediment to current treatments of many malignant ***,an antioxidant enzyme,is capable of decomposing endogenous hydrogen peroxide(H2O2)into oxygen for tumor reoxygenation,but suffered from in vivo instability and limited delivery to deep interior hypoxic regions in ***,a deep-penetrated nanocatalase-loading DiIC18(5,DiD)and soravtansine(Cat@PDS)were provided by coating catalase nanoparticles with PEGylated phospholipids membrane,stimulating the structure and function of erythrocytes to relieve tumor hypoxia for enhanced chemophotodynamic *** intravenous administration,Cat@PDS preferentially accumulated at tumor sites,flexibly penetrated into the interior regions of tumor mass and remarkably relieved the hypoxic status in ***,the Cat@PDS+laser treatment produced striking inhibition of tumor growth and resulted in a 97.2%suppression of lung ***,the phospholipids membrane-coated nanocatalase system represents an encouraging nanoplatform to relieve tumor hypoxia and synergize the chemophotodynamic cancer therapy.

关键词： Photodynamic therapy Tumor hypoxia Catalase Tumor penetration Drug delivery Nanoparticle Tumor targeting Cancer metastasis Cancer therapy

First-principles calculations and experimental studies of Sn-Zn alloys as negative electrode materials for lithium-ion batteries

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Rare Metals 2011年第2期30卷 160-165页

作者： RU Qiang PENG Wei zhang zhiwen HU Shejun LI Yanling Laboratory of Quantum Information Technology School of Physics and Telecommunication Engineering South China Normal University Guangzhou 510006 China

The physical characters and electrochemical properties of various phases in a Sn-Zn electrode, such as formation energy, plateau potential, specific capacity, as well as volume expansion, were calculated by the first-principles plane-wave pseudo-potential method based on the den- sity functional theory. Sn-Zn films were also deposited on copper foils by an electroless plating technique. The actual composition and chemical characters were explored by scanning electron microscopy （SEM）, X-ray diffraction （XRD）, plasma atomic emission spectrometry （ICP）, and constant current charge/discharge measurements （CC）. The results show that separation phases with tin and zinc including a small quantity of Cu6Sn5 phase were obtained, the initial lithium insertion capacity of the Sn-Zn film was 661 mAh/g, and obvious potential pla- teaus of about 0.4 V and 0.7 V were displayed, which is in accordance with the results of theoretical calculations. The capacity of the Sn-Zn film decreased seriously with the increase of cycle number.

关键词： lithium batteries electrochemical electrodes tin alloys first-principles calculations

Androgen receptor coregulator ARA267-α interacts with death receptor-6 revealed by the yeast two-hybrid

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Science China(Life Sciences) 2004年第5期47卷 442-448页

作者： MAI Tiejun WANG Xin zhang zhiwen XIN Dianqi NA Yanqun GUO Yinglu Institute of Urology First Hospital of Peking UniversityBeijing 100034China Department of General Surgery First Hospital of Peking UniversityBeijing 100034China Department of Physiology Peking UniversityBeijing 100083China

ARA267-a is a newly identified androgen receptor coactivator. In order to further elucidate its precise role in cells, using the ARA267- a fragment containing four PHD and one SET conserved domains as bait we revealed an ARA267-a-PHD-SET-interacting protein, death receptor-6 (DR6), in the yeast two-hybrid screening. DR6 is the member of TNF receptor family and has a death domain in its intracellular cytoplasmic portion (DR6cp) to mediate the cell apoptosis. The interaction between ARA267-a-PHD-SET and DR6cp was confirmed in vitro and in vivo. Our finding implied that androgen signaling pathway might cross talk with apoptosis sig-naling pathway through the interaction between ARA267-a and DR6.

关键词： ARA267-a androgen receptor androgen receptor coregulator death receptor-6 yeast two-hybrid system

Use of suppression subtractive hybridization strategy for cloning and identifying specifically expressed genes of renal cell carcinoma

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Chinese Science Bulletin 2001年第3期46卷 226-229页

作者： zhang Qiang zhang zhiwen GONG Kan XIN Dianqi NA Yanqun GUO Yinlu Department of Urology and Institute of Urology The First Affiliated Hospital of Beijing Medical UniversityBeijing 100034China Department of Physiology Beijing Medical UniversityBeijing 100083China

Using suppression subtractive hybridization, a renal cell carcinoma (RCC) cDNA subtractive library which only contains differently expressed cDNAs between human RCC and normal kidney has been constructed. 200 clones were picked out randomly to perform enzyme digest analysis, a part of them underwent sequence analysis and Northern blot to identify RCC specially expressed genes. Results showed that 190 clones contain 50-400 bp inserts respectively. Sequence analysis was performed in 10 clones. All the 10 sequences were unknown before and derived from 6 unique novel genes among which the cDNA insert RCC18 has five copies. Northern blot analysis showed that RCC18 cDNA expressed highly in RCC, but there was no signal detected in normal kidney, and the full length of RCC18 was about 2.5 kb. The constructed cDNA subtractive library of human RCC is a highly efficient one and lays the solid foundation for large-scale screening and cloning new and specific oncogenes or tumor suppressor genes of RCC. The novel

关键词： kidney neoplasms carcinoma suppression subtractive hybridization gene library.

Aberrant activity of Wnt/Frizzled signaling pathway in renal cancer cell lines

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Chinese Science Bulletin 2000年第18期45卷 1703-1707页

作者： ZANG Tong ZHUANG Liyan zhang zhiwen XIN Dianqi GUO Yinglu Institute of Urology First Teaching HospitalBeijing Medical UniversityBeijing 100034China Department of Physiology Beijing Medical UniversityBeijing 100083China

The expression of Wnt, Wnt receptor-Frizzled, and several other key components in Wnt pathway in renal cancer cell lines was studied. The result of semi-quantitative RT-PCR has shown that the expression level of Wnt5A and hFz5 mRNA were higher in renal cancer cell line (GRC-1) than in normal renal cell line (HK-2). This result has been confirmed by in situ hybridization. The expression of p-catenin protein was obviously higher in GRC-1 than in HK-2 (P<0.01), but there were no different expressions of its mRNA between 3 lines. The reasons of the overexpression of β-catenin has been investigated by means of immunocytochemistry, SSCP and so on, no mutation of β-catenin gene and APC were found. That means that the overexpression of Wnt5A/hFz5 might be the reason of overexpression of β-catenin. It was concluded that the aberrant activity of Wnt pathway might play an important role in renal cell carcinoma.

关键词： Wnt/Frizzled β-catenin signaling pathway renal cell carcinoma.

Cloning and mRNA expression of macrophage migration inhibitory factor (MIF) gene of large yellow croaker (P seudosciaena crocea)

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Acta Oceanologica Sinica 2010年第3期29卷 63-73页

作者： MAO Yong XU Bing SU Yongquan zhang zhiwen DING Shaoxiong WANG Ding WANG Jun Fisheries College of Guangdong Ocean University Zhanjiang 524025 China State Key Laboratory of Marine Environmental Science Xiamen University Xiamen 361005 China

Mammalian macrophage migration inhibitory factor （MIF） plays an important role as an indispensable mediator in the pathogenesis of inflammatory disease like septicemia, but little is known about the role of MIF homologue in fish septicemia. The authors have cloned the MIF homologue in large yellow croaker Pseudosciaena crocea （LycMIF） using RACE approach. The full-length cDNA of LycMIF was 634 bases and contained an ORF of 345 bases encoding a protein of 115 amino acid residues. As demonstrated by RT-PCR and QRT-PCR assay, MIF mRNAs were constitutively expressed in 11 selected tissues and were abundant in brain and liver. Moreover, the LycMIF transcripts in the liver and head kidney were responsive to bacteria infection and could be significantly up-regulated. Our results provide the first direct evidence that fish MIF was implicated in pathogenesis of fish vibrosis and play an important role in response to bacteria infection.

关键词： macrophage migration inhibitory factor (MIF) vibriosis large yellow croaker mRNA expression

Metallothionein expression induced by nickel accumulation in the midgut of Spodoptera litura Fabricius larvae exposed to nickel

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Chinese Science Bulletin 2007年第23期52卷 3227-3232页

作者： SUN HongXia ZHOU Qiang TANG WenCheng SHU YingHua ZOU zhiwen zhang GuRent State key laboratory for Biological Control & Institute of Entomology Sun Yat-sen University Guangzhou 510275 China

Metallothionein expression induced by nickel accumulation in the midgut of Spodoptera litura Fabri- cius larvae was investigated by exposing S. litura larvae to the artificial diets amended with different doses of nickel for 3 generations. Nickel accumulations in the midgut of 6th instar larvae were detected by Inductively Coupled Plasma-Atomic Emission Spectrometer (ICP-AES), and the induced metal- lothionein expression in the midgut of 5th and 6th instar larvae in 120 h were quantified using cad- mium-hemoglobin total saturation methods. The accumulated nickel in the midgut of the 6th instar larvae within a generation increased with the increase of the nickel doses in the treated diets and showed significant dose-dependence with the nickel doses in diets. Metallothioneins induced by nickel accumulated in the larval midgut also increased with nickel doses in diets and with the increasing stressing time in a generation. Results also suggested that the metallothionein expression was differ- ent with the time of S. litura larvae stressed by nickel.

关键词：镍元素亲金属蛋白质中肠物质积累

An Adaptive ANOVA-Based Data-Driven Stochastic Method for Elliptic PDEs with Random Coefficient

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Communications in Computational Physics 2014年第8期16卷 571-598页

作者： zhiwen zhang Xin Hu Thomas Y.Hou Guang Lin Mike Yan Computing and Mathematical Sciences California Institute of TechnologyPasadenaCA 91125USA. Pacific Northwest National Laboratory RichlandWA 99352USA.

In this paper,we present an adaptive,analysis of variance(ANOVA)-based data-driven stochastic method(ANOVA-DSM)to study the stochastic partial differential equations(SPDEs)in the multi-query *** new method integrates the advantages of both the adaptive ANOVA decomposition technique and the data-driven stochastic *** handle high-dimensional stochastic problems,we investigate the use of adaptive ANOVA decomposition in the stochastic space as an effective dimension-reduction *** improve the slow convergence of the generalized polynomial chaos(gPC)method or stochastic collocation(SC)method,we adopt the data-driven stochastic method(DSM)for speed *** essential ingredient of the DSM is to construct a set of stochastic basis under which the stochastic solutions enjoy a compact representation for a broad range of forcing functions and/or boundary *** ANOVA-DSM consists of offline and online *** the offline stage,the original high-dimensional stochastic problem is decomposed into a series of lowdimensional stochastic subproblems,according to the ANOVA decomposition ***,for each subproblem,a data-driven stochastic basis is computed using the Karhunen-Lo`eve expansion(KLE)and a two-level preconditioning optimization *** trial functions are used to enrich the stochastic basis and improve the *** the online stage,we solve each stochastic subproblem for any given forcing function by projecting the stochastic solution into the data-driven stochastic basis constructed *** our ANOVA-DSM framework,solving the original highdimensional stochastic problem is reduced to solving a series of ANOVA-decomposed stochastic subproblems using the *** adaptive ANOVA strategy is also provided to further reduce the number of the stochastic subproblems and speed up our *** demonstrate the accuracy and efficiency of our method,numerical examples are presented for one-and two-dimensional elliptic PDEs with rando

关键词： Analysis of variance stochastic partial differential equations data-driven methods Karhunen-Loeve expansion uncertainty quantification model reduction.

Mathematical Model of a Bacteria-Immunity System with the Influence of Quorum Sensing Signal Molecule

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Journal of Applied Mathematics and Physics 2016年第5期4卷 888-896页

作者： zhiwen zhang School of Sciences Xi’an University of Science and Technology Xi’an China

A recent study illustrates an exciting new element of bacteria-host interactions in which the opportunistic pathogen Pseudomonas aeruginosa quorum sensing signal molecule can modulate the host immune system. In this paper, on the basic of a model which describes the competition between bacteria and immune system, a mathematical model is proposed to investigate the modulation of quorum sensing signal molecule on immune response. It is shown that the modulation leads to a backward bifurcation and some more new dynamical behaviors. By numerical simulations, we find a fold bifurcation of equilibria, which induces three patterns of dynamical behaviors including the bistable phenomenon. Our model quantifies the modulation of quorum sensing signal molecule on the immune response, which may provide a therapy for bacteria infections.

关键词： Pseudomonas aeruginosa Quroum Sensing Modulation Bifurcation

First-principle study on NiSn_(0.5)Ti_(0.5) phase as electrode materials for lithium ion battery

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Chinese Science Bulletin 2010年第27期55卷 3113-3117页

作者： RU Qiang HU SheJun zhang zhiwen PENG Wei HOU XianHua HUANG ZhaoWen Laboratory of Quantum Information Technology School of Physics and Telecommunication Engineering South China Normal University Guangzhu 510006 China Department of Mathematics and Physics Wuyi University Jiangmen 529020 China

The electronic structure and electrochemical parameters of ternary NiSn0.5Ti0.5 phase are investigated by plane-wave pseudopotential method of the *** interstitial sites are firstly filled with lithium atoms,and then the substitution for Ni sites *** results show that the Fermi level of lithium-intercalated phase goes up with the increasing lithium *** interactions of M-M covalent bonds (M=Ni,Sn,Ti) become weak,while Li-M bonds are found to *** 0.5Ti0.5 phase is characterized with high theoretical capacity and low voltage of *** obvious volume effect from 77.15%to 189.94% would lead to the failure of the electrode material,and therefore the NiSn0.5Ti0.5 compound should be limited as low as possible in multi-element Sn-based alloy.

关键词：锂离子电池电极材料一阶锡基合金电化学参数第一性原理 TiAl基电子结构