Cloning and mRNA expression of macrophage migration inhibitory factor (MIF) gene of large yellow croaker (P seudosciaena crocea)

作     者：MAO Yong XU Bing SU Yongquan ZHANG Zhiwen DING Shaoxiong WANG Ding WANG Jun 

作者机构：Fisheries College of Guangdong Ocean University Zhanjiang 524025 China State Key Laboratory of Marine Environmental Science Xiamen University Xiamen 361005 China 

出 版 物：《Acta Oceanologica Sinica》 (海洋学报（英文版）)

年 卷 期：2010年第29卷第3期

页      面：63-73页

核心收录：

学科分类：071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 0908[农学-水产] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 090102[农学-作物遗传育种] 0710[理学-生物学] 0707[理学-海洋科学] 0817[工学-化学工程与技术] 0901[农学-作物学] 0836[工学-生物工程] 

基　　金：The National Natural Science Foundation of China under contract No. 40976096 the National Department Public Benefit Research Foundation of China under contract No. 200903029 the National High Technology Research and Development Program of China (863 Program) under contract No. 2006AA10A405 

主　　题：macrophage migration inhibitory factor (MIF) vibriosis large yellow croaker mRNA expression 

摘      要：Mammalian macrophage migration inhibitory factor （MIF） plays an important role as an indispensable mediator in the pathogenesis of inflammatory disease like septicemia, but little is known about the role of MIF homologue in fish septicemia. The authors have cloned the MIF homologue in large yellow croaker Pseudosciaena crocea （LycMIF） using RACE approach. The full-length cDNA of LycMIF was 634 bases and contained an ORF of 345 bases encoding a protein of 115 amino acid residues. As demonstrated by RT-PCR and QRT-PCR assay, MIF mRNAs were constitutively expressed in 11 selected tissues and were abundant in brain and liver. Moreover, the LycMIF transcripts in the liver and head kidney were responsive to bacteria infection and could be significantly up-regulated. Our results provide the first direct evidence that fish MIF was implicated in pathogenesis of fish vibrosis and play an important role in response to bacteria infection.