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Lack of fitness cost and inheritance of resistance to abamectin based on the establishment of a near-isogenic strain of Tetranychus urticae

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Journal of Integrative Agriculture 2023年第6期22卷 1809-1819页

作者： zhang Yan TIAN Tian zhang Kun zhang you-jun WU Qing-jun XIE Wen GUO Zhao-jiang WANG Shao-li State Key Laboratory of Vegetable Biobreeding Institute of Vegetables and FlowersChinese Academy of Agricultural SciencesBeijing 100081P.R.China College of Agriculture Yangtze UniversityJingzhou 434025P.R.China Sanya Nanfan Research Institute Hainan UniversitySanya 572024P.R.China

Many populations of the two-spotted spider mite, Tetranychus urticae Koch, have developed high levels of resistance to the pesticide abamectin in China and other countries. This study developed a near-isogenic line to understand better the inheritance, cross-resistance, and fitness costs associated with abamectin resistance in the field population of T. urticae in China. We introduced the trait that confers extremely high abamectin resistance in a field-collected population of T. urticae into a susceptible laboratory strain(IPP-SS) to generate an abamectin-resistant near-isogenic line(NIL-Aba).This process was carried out through multiple backcrossing to IPP-SS and via parthenogenesis and abamectin screening. Compared with IPP-SS, the NIL-Aba strain had a 25 147-fold resistance to abamectin and a high level of cross-resistance to bifenthrin(288.17-fold), an intermediate level to emamectin benzoate(42.57-fold), and low levels to bifenazate, chlorfenapyr, cyflumetofen, cyenopyrafen, and cyetpyrafen with resistance ranging from 3.18-to *** it had no cross-resistance to profenofos. The resistance to abamectin in NIL-Aba was autosomal, incompletely dominant, and polygenic. Based on two sex life table parameters, no fitness cost was found in NIL-Aba. Establishing the NIL-Aba strain provides a reliable basis for an in-depth study of abamectin resistance in T. urticae. New information on toxicological characteristics and fitness cost should facilitate the management of abamectin resistance in field populations of T. urticae.

关键词： two-spotted spider mite abamectin resistance near-isogenic strain inheritance pattern fitness cost

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Characterization of the chemosensory protein EforCSP3 and its potential involvement in host location by Encarsia formosa

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Journal of Integrative Agriculture 2023年第2期22卷 514-525页

作者： WANG Ke HE Yan-yan zhang you-jun GUO Zhao-jiang XIE Wen WU Qing-jun WANG Shao-li Institute of Vegetables and Flowers Chinese Academy of Agricultural SciencesBeijing 100081P.R.China

Chemosensory proteins(CSPs) perform several functions in *** study performed the gene expression,ligand-binding,and molecular docking assays on the EforCSP3 identified in the parasitoid wasp Encarsia formosa,to determine whether EforCSP3 functions in olfaction,especially in host location and host *** results showed that EforCSP3 was highly expressed in the female head,and its relative expression was much higher in adults than in other developmental *** fluorescence binding assays suggested that the EforCSP3 exhibited high binding affinities to a wide range of host-related volatiles,among which dibutyl phthalate,1-octene,β-elemene,and tridecane had the strongest binding affinity with EforCSP3,besides α-humulene and β-myrcene,and should be assessed as potential *** structure modeling and molecular docking predicted the amino acid residues of EforCSP3possibly involved in volatile binding.α-Humulene and β-myrcene attracted *** in a previous study and exhibited strong binding affinities with EforCSP3 in the current *** conclusion,EforCSP3 may be involved in semiochemical reception by ***.

关键词： Encarsia formosa chemosensory protein expression profiles fluorescence binding assay molecular docking

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Comparative Analysis of Population Genetic Structure in Bemisia tabaci(Gennadius) Biotypes B and Q Based on ISSR Marker

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Agricultural Sciences in China 2008年第11期7卷 1348-1354页

作者： CHU Dong WAN Fang-hao XU Bao-yun WU Qing-jun zhang you-jun High-Tech Research Center Shandong Academy of Agricultural Sciences Jinan 250100 P.R.China Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences Beijing 100081 P.R. China The State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Scienees Beijing 100081 P.R. China

Bemisia tabaci （Gennadius） biotypes B and Q are two invasive biotypes in the species complex. The comparison of the population genetic structure of the two biotypes is of significance to show their invasive mechanism and to their control. The intersimple sequence repeats （ISSR） marker was used to analyze the 16 B-biotype populations and 4 Q-biotype populations worldwide with a Trialeurodes vaporariorum population in Shanxi Province, China, and a B. tabaci non-B/Qbiotype population in Zhejiang Province, China, was used as control populations. The analysis of genetic diversity showed that the diversity indexes of biotype Q including Nei＇s gene diversity index, Shannon informative index, and the percentage of polymorphic loci were higher than those of biotype B. The high genetic diversity of biotype Q might provide the genetic basis for the excellent ecological adaptation. Cluster analysis suggested that the ISSR could not be used in the phylogenetic analysis though it could easily distinguish the biotypes of B. tabaci. The difference of the population genetic structure between the biotype B and the biotype Q exists based on the ISSR marker. Meanwhile, the results suggested that the molecular marker has its limitation in the phylogenetic analysis among the biotypes of B. tabaci.

关键词： invasive mechanism Bemisia tabaci biotype B Bemisia tabaci biotype Q genetic structure ISSR

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CRISPR/Cas9-based functional characterization of Px ABCB1 reveals its roles in the resistance of Plutella xylostella(L.)to Cry1Ac,abamectin and emamectin benzoate

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Journal of Integrative Agriculture 2023年第10期22卷 3090-3102页

作者： OUYANG Chun-zheng YE Fan WU Qing-jun WANG Shao-li Neil CRICKMORE ZHOU Xu-guo GUO Zhao-jiang zhang you-jun State Key Laboratory of Vegetable Biobreeding Deapartment of Plant ProtectionInstitute of Vegetables and FlowersChinese Academy of Agricultural SciencesBeijing 100081P.R.China Guangdong Laboratory for Lingnan Modern Agriculture Guangzhou 510642P.R.China School of Life Sciences University of SussexBrighton BN19QGUK Department of Entomology University of KentuckyLexingtonKY 40546-0091USA

The identification of functional midgut receptors for pesticidal proteins produced by Bacillus thuringiensis(Bt)is critical for deciphering the molecular mechanism of Bt resistance in *** expression of the PxABCB1 gene was previously found to be associated with Cry1Ac resistance in the diamondback moth,Plutella xylostella(L.).To directly validate the potential receptor role of PxABCB1 and its contribution to Bt Cry1Ac toxicity in ***,we used CRISPR/Cas9 to generate a homozygous knockout ABCB1KO strain with a 5-bp deletion in exon 3 of its *** ABCB1KO strain exhibited a 63-fold resistance to Cry1Ac toxin compared to the parental DBM1Ac-S ***,the ABCB1KO strain also exhibited significant increases in susceptibility to abamectin and emamectin *** changes in susceptibility to various other Bt Cry proteins or synthetic insecticides were *** knockout strain exhibited no significant fitness ***,our study indicates that PxABCB1 can protect the insect against avermectin insecticides on one hand,while on the other hand it facilitates the toxic effect of the Bt Cry1Ac *** results of this study will help to inform integrated pest management approaches against this destructive pest.

关键词： Bacillus thuringiensis Plutella xylostella CRISPR/Cas9 ABCB1 bioinsecticide resistance

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Genetic Differentiation of Different Geographical Populations of Bemisia tabaci (Gennadius) Complex

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Agricultural Sciences in China 2007年第6期6卷 696-705页

作者： CHU Dong LIU Guo-xia FAN Zhong-xue TAO Yun-li zhang you-jun High-Tech Research Center Shandong Academy of Agricultural Sciences Jinan 250100 P.R.China Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China

Bemisia tabaci （Gennadius） is a species complex, which includes different geographical populations with genetic differentiation. The recent progress on the genetic differentiation of various geographical populations of B. tabaci complex was introduced. The genetic differentiation was further analyzed on the basis of the sequences of mtDNA COI and rDNA ITS1 recorded in the world＇s GenBank. Five groups are defined on the basis of mtDNA COI and rDNA ITS1, including the Asia group, America group, Africa group, Australia group, and Biotype B/Mediterranean/Middle East/ Northern Africa/Biotype Ms group. There are several ungrouped geographical classifications, such as the Uganda population, Ivory Coast population, and Taiwan population. Geographical isolation may be the most important factor that contributed to the genetic differentiation of various geographical populations of B. tabaci. Many populations with biological advantages invaded new regions and caused severe economic losses within human activity. It is necessary to strengthen the research of B. tabaci biotype to prevent the spread of invaded populations and the invasion of potentially dangerous populations.

关键词： Bemisia tabaci (Gennadius) geographical populations mtDNA COI rDNA ITS 1 genetic differentiation

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A survey on the off-target effects of insecticidal double-stranded RNA targeting the Hvβ'COPI gene in the crop pest Henosepilachna vigintioctopunctata through RNA-seq

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Journal of Integrative Agriculture 2022年第9期21卷 2665-2674页

作者： Lü Jing Satyabrata NANDA CHEN Shi-min MEI Yang HE Kang QIU Bao-li zhang you-jun LI Fei PAN Hui-peng Key Laboratory of Bio-pesticide Innovation and Application of Guangdong Province South China Agricultural UniversityGuangzhou 510642P.R.China MS Swaminathan School of Agriculture Centurion University of Technology and ManagementParalakhemundi 761211India Institute of Insect Sciences College of Agriculture and BiotechnologyZhejiang UniversityHangzhou 310058P.R.China Department of Plant Protection Institute of Vegetables and FlowersChinese Academy of Agricultural SciencesBeijing 100081P.R.China

The specificity of the double-stranded RNA(dsRNA) used in the RNA interference(RNAi) technique is crucial for the success of sequence-specific gene silencing. Currently, RNAi-mediated insect control is a trending research ***, the off-target effects of the dsRNA in RNAi are a major concern. In this study, the ds Hvβ’COPI(coat protein complex I, β’ subunit)-treated and untreated transcriptomes of the 28-spotted potato lady beetle(Henosepilachna vigintioctopunctata) were compared to understand its off-target gene silencing effects. The RNA-seq results revealed that 63 and 44 differentially expressed genes(DEGs) were upregulated and downregulated, respectively, in the ds Hvβ’COPI treated group as compared with the control. Validation of the differential expressions of some selected DEGs via reverse transcription-quantitative PCR(RT-qPCR) analysis confirmed the reliability of the transcriptome analysis results. Further downstream analysis revealed that there were no genes homologous with Hvβ’COPI in H. vigintioctopunctata. Additionally,no genes with a >11 bp continuous match with ds Hvβ’COPI were found in the H. vigintioctopunctata transcriptome. Six genes(Hvcitron, Hvhelicase, Hvtransposase, Hvserine, Hvdynein, and Hv E3 ubiquitin) were selected to examine the offtarget activity of ds Hvβ’COPI based on their potential involvement in various H. vigintioctopunctata metabolic *** severity of silencing these six off-target genes was evaluated by employing RNAi. The RNAi results confirmed the downregulation of the expression of all six genes, although there was no significant lethality. The findings of this study will be helpful in the risk analysis of future RNAi-mediated pest control experiments.

关键词： RNAi dsRNA off-target effects RNA-seq transcriptome analysis

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T形开槽三角形贴片双模微带带通滤波器

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北京理工大学学报 2011年第6期31卷 695-698页

作者：张友俊杨磊上海海事大学信息工程学院上海201306

基于传统三角形双模贴片微带带通滤波器,提出了一种新型的贴片上开槽的等腰三角形双模带通滤波器.在等腰三角形贴片谐振器内部挖出一个T形槽,并对其进行了优化,优化后的滤波器能在通带两侧都产生衰减极点,提高了阻带的抑制能力,同时保... 详细信息

基于传统三角形双模贴片微带带通滤波器,提出了一种新型的贴片上开槽的等腰三角形双模带通滤波器.在等腰三角形贴片谐振器内部挖出一个T形槽,并对其进行了优化,优化后的滤波器能在通带两侧都产生衰减极点,提高了阻带的抑制能力,同时保证了滤波器的小型化.对该结构进行仿真,仿真结果表明通带的中心频率为5GHz,与实测结果相一致.

关键词：微带带通滤波器双模三角形贴片滤波器

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《道行般若经》中特殊的“而”字考察

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古汉语研究 2021年第4期 108-115,128页

作者：张幼军湖南师范大学文学院湖南长沙410081

《道行般若经》译出早在东汉,其中有些"而"字的用法有别于常。其特殊用法包括句法和词法两方面。句法方面主要表现在置于特殊的句法位置,包括主谓之间、动宾之间和"于……"后;词法方面主要表现在用于特殊的词语中,包括用在词语"无而"和... 详细信息

《道行般若经》译出早在东汉,其中有些"而"字的用法有别于常。其特殊用法包括句法和词法两方面。句法方面主要表现在置于特殊的句法位置,包括主谓之间、动宾之间和"于……"后;词法方面主要表现在用于特殊的词语中,包括用在词语"无而"和"何而"中。

关键词：《道行般若经》 “而” 特殊用法

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论人类学的经济学

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广西民族学院学报（哲学社会科学版） 2005年第6期27卷 47-53页

作者：张有隽广西民族学院民族学人类学研究所

经济人类学是人类学中与经济学对话的部分。它以人类的经济行为为研究对象,但与经济学对经济的理解不同。由于经济是嵌合在社会制度中的,经济人类学必须从人类全盘的风俗和制度中来着手自己的研究。它的领域比较广泛,不仅描述人们的生... 详细信息

经济人类学是人类学中与经济学对话的部分。它以人类的经济行为为研究对象,但与经济学对经济的理解不同。由于经济是嵌合在社会制度中的,经济人类学必须从人类全盘的风俗和制度中来着手自己的研究。它的领域比较广泛,不仅描述人们的生产、交换、分配和消费方式,以及这些系统如何组织、动作,还包括建立理论来描述制度脉络和影响,经济运作的动力等。

关键词：人类学经济学经济人类学制度脉络

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基于信号干扰理论的差分带通滤波器

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吉林大学学报（工学版） 2017年第3期47卷 1003-1008页

作者：张友俊刘晓元上海海事大学信息工程学院上海201306

基于信号干扰理论,提出了一种差分宽带带通滤波器结构。由于该滤波器结构具有互补对称性,使该滤波器在差模激励时表现为带通滤波器,在共模激励时表现为带阻滤波器。另外,共模激励时,输入/输出端口之间有两条电长度不同的传输路径,使得... 详细信息

基于信号干扰理论,提出了一种差分宽带带通滤波器结构。由于该滤波器结构具有互补对称性,使该滤波器在差模激励时表现为带通滤波器,在共模激励时表现为带阻滤波器。另外,共模激励时,输入/输出端口之间有两条电长度不同的传输路径,使得共模信号在整个通带范围内得到很好的抑制。试验结果表明:差模通带中心频率f0为7.85GHz;最大回波损耗低于-25dB;3dB相对带宽为61%(5.5~10.2GHz);通带内插入损耗最小可达0.2dB。在差模通带内,共模抑制最小可达-20dB,其中-20dB抑制共模阻带带宽可覆盖5.5~10.2GHz,实测结果与仿真结果吻合。

关键词：电磁学差分带通滤波器信号干扰理论差模共模

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