Characterization of the chemosensory protein EforCSP3 and its potential involvement in host location by Encarsia formosa

作     者：WANG Ke HE Yan-yan ZHANG You-jun GUO Zhao-jiang XIE Wen WU Qing-jun WANG Shao-li WANG Ke;HE Yan-yan;ZHANG You-jun;GUO Zhao-jiang;XIE Wen;WU Qing-jun;WANG Shao-li

作者机构：Institute of Vegetables and FlowersChinese Academy of Agricultural SciencesBeijing 100081P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2023年第22卷第2期

页      面：514-525页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学] 

基　　金：supported by the National Natural Science Foundation of China (31772172) the earmarked fund for China Agriculture Research System (CARS25) the Beijing Key Laboratory for Pest Control and Sustainable Cultivation of Vegetables 

主　　题：Encarsia formosa chemosensory protein expression profiles fluorescence binding assay molecular docking 

摘      要：Chemosensory proteins(CSPs) perform several functions in *** study performed the gene expression,ligand-binding,and molecular docking assays on the EforCSP3 identified in the parasitoid wasp Encarsia formosa,to determine whether EforCSP3 functions in olfaction,especially in host location and host *** results showed that EforCSP3 was highly expressed in the female head,and its relative expression was much higher in adults than in other developmental *** fluorescence binding assays suggested that the EforCSP3 exhibited high binding affinities to a wide range of host-related volatiles,among which dibutyl phthalate,1-octene,β-elemene,and tridecane had the strongest binding affinity with EforCSP3,besides α-humulene and β-myrcene,and should be assessed as potential *** structure modeling and molecular docking predicted the amino acid residues of EforCSP3possibly involved in volatile binding.α-Humulene and β-myrcene attracted *** in a previous study and exhibited strong binding affinities with EforCSP3 in the current *** conclusion,EforCSP3 may be involved in semiochemical reception by ***.