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Identification of processed Chinese medicinal materials using DNA mini-barcoding

Chinese Journal of Natural Medicines 2017年第7期15卷 481-486页

作者： SONG Ming DONG Gang-Qiang zhang ya-qin LIU Xia SUN Wei Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences Beijing 100700 China School of Chemistry Chemical Engineering and Life Sciences Wuhan University of Technology Wuhan 430070 China Amway (China) Botanical Research and Development Center Wuxi 214145 China

Most of Chinese medicinal herbs are subjected to traditional processing procedures, including stir-frying, charring, steaming, boiling, and calcining before they are released into dispensaries. The marketing and identification of processed medicinal materials is a growing issue in the marketplace. However, conventional methods of identification have limitations, while DNA mini-barcoding, based on the sequencing of a short-standardized region, has received considerable attention as a new potential means to identify processed medicinal materials. In the present study, six DNA barcode loci including ITS2, psb A-trn H, rbc L, mat K, trnL(UAA) intron and its P6 loop, were employed for the authentication of 45 processed samples belonging to 15 species. We evaluated the amplification efficiency of each locus. We also examined the identification accuracy of the potential mini-barcode locus, of trnL(UAA) intron P6 loop. Our results showed that the five primary barcode loci were successfully amplified in only 8.89%——20% of the processed samples, while the amplification rates of the trnL(UAA) intron P6 loop were higher, at 75.56% successful amplification. We compared the mini-barcode sequences with Genbank using the Blast program. The analysis showed that 45.23% samples could be identified to genus level, while only one sample could be identified to the species level. We conclude that trnL(UAA) p6 loop is a candidate mini-barcode that has shown its potential and may become a universal mini-barcode as complementary barcode for authenticity testing and will play an important role in medicinal materials control.

关键词： DNA barcoding trnL(UAA)intron P6 loop Identification Processed medicinal materials Mini-barcode

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Population dynamics and protection strategies of Litsea coreana *** with Guizhou Province in China as an example

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Journal of Mountain Science 2023年第4期20卷 1090-1101页

作者： YUAN Gang-yi GUO Qi-qiang XIE Na zhang ya-qin LUO Si-qiong GUI qin yaO Shan Institute for Forest Resources and Environment of Guizhou College of ForestryGuizhou UniversityGuiyang550025China

Studying the dynamics of plant populations is crucial to the conservation and management of endangered *** diameter at breast height(DBH),tree height,crown width,and environmental factors of a total of 15 plots in five populations in Guizhou Province,China were measured in this *** method of substituting diameter at breast height for age was used to divide the population into 7 age *** age structure,static life table,and relationship between the population characteristics and environmental factors of the Litsea coreana *** population were ***,the time series model was used to predict the population *** showed that the age structure of population in Zhengan County(Population ZA)had an inverted Jshape with abundant seedlings and insufficient middle-aged and elderly *** age structures of populations in Daozhen County(Population DZ),Kaiyang County(KY),Meitan County(Population MT),and Xishui County(Population XS)were spindle-shaped with few young individuals and many middle-aged and elderly *** mortality rate of each population fluctuated with the increase in age class,and the populations were extremely *** survival curve was close to the Deevey III type,and the degree of deviation was related to the lack of young *** the age stages II,IV,and VI,the number of aged plants in the different populations initially increased then decreased,and population stability was difficult to *** analysis showed that the DBH,tree height and crown width of the population decreased with the increase of annual mean temperature,and the population density was restricted by soil nutrient conditions to a certain *** results of this work provide scientific basis for the conservation and sustainable utilization of natural populations of *** *** in Guizhou Province,China.

关键词： Litsea coreana var.sinensis Age structure Survival curve Time series prediction Environmental factor

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Effect of three cooking methods on nutrient components and antioxidant capacities of bamboo shoot(Phyllostachys praecox C.D. Chu et C.S. Chao)

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第9期12卷 752-759页

作者： Jin-jie zhang Rong JI ya-qin HU Jian-chu CHEN Xing-qian YE Department of Food Science and Nutrition College of Biosystem Engineering and Food ScienceZhejiang UniversityHangzhou 310029China

Three cooking methods,namely boiling,steaming,and stir-frying for 5 to 10 min,were used to evaluate the effect on nutrient components,free amino acids,L-ascorbic acid,total phenolic contents,and antioxidant capacities of bamboo shoots(Phyllostachys praecox).Results showed that boiling and stir-frying had a great effect on the nutrient components and they decreased the contents of protein,soluble sugar,and ash,and caused a great loss in the total free amino acids(decreased by 38.35% and 34.86%,respectively).Significant differences(P<0.05) in free amino acids were observed in the samples cooked by different ***-fried bamboo shoots had a high fat content which increased by 528.57% because of the addition of edible *** boiling,the L-ascorbic acid and total phenolic contents were significantly reduced,while steaming increased total phenolic content by 3.98% and stir-frying well-preserved L-ascorbic acid(78.87% of its previous content).Results of the antioxidative property study showed that stir-frying could increase antioxidant capacities of bamboo *** is concluded that stir-frying is more suitable for bamboo shoots because it could obtain the maximum retention of antioxidant capacities.

关键词： Cooking methods Bamboo shoot Nutrition Antioxidant capacities

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Clinical significance of multislice spiral CT scans in hepatic veins occlusion in Budd-Chiari syndrome

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Chinese Medical Journal 2007年第2期120卷 100-105页

作者： MENG Xiao-chun ZHU Kang-shun qin Jie zhang Jian-sheng WANG Xiao-hong ZOU yan zhang ya-qin SHAN Hong Department of Radiology Third Affiliated Hospital Sun Yat-sen University Guangzhou 510630 China

Background Budd-Chiari syndrome with hepatic vein occlusion （HVBCS） can induce severe portal hypertension and liver damage. We retrospectively analyzed hepatic CT features of HVBCS and evaluated the usefulness of triphasic enhancement of CT examinations and CT angiography （CTA） in its diagnosis. Methods Twenty-five cases with HVBCS, confirmed by digital subtraction angiography （DSA）, received a triphasic enhancement CT scan within one week before DSA. The CTA images of the relevant blood vessels were reconstructed with maximum intensity projection, volume rendering and oblique reformat techniques. Results Compared with DSA, the detection rate of transverse CT and CTA images for abnormal hepatic vein were 81.7% （58/71） and 95.8% （68/71）（X^2=7.044, P=-0.008）, for membranous obstruction were 47.4% （9/19） and 84.2% （16/19） respectively （X^2 =5.729, P=-0.017 ）, for segmental obstruction were 88.0% （22/25） and 100% （25/25） respectively （X^2=1.418, P=-0.234）. The detection rates for hepatic vein stenosis were 100% with each method. Diffuse hepatomegaly was found in all 6 cases in acute phase and 3 of 19 cases in chronic phase who had severe obstruction of three hepatic veins without patent intrahepatic collaterals. The other 16 cases in chronic phase had hepatatrophia to different extents related to the obstructed hepatic vein. All in acute phase and 15 in chronic phase presented typical patchy enhancement initially in caudate lobe and perihilar areas and enlarged with time delay. In all cases, parenchyma areas with atrophy, necrosis and congestion demonstrated lower and later enhancement. In all the parts, which had normal enhancement at least one patent outflow hepatic vein, accessory hepatic vein or collateral vessel was detected.Conclusion Dynamic enhancement CT examination by multislice spiral CT not only could improve the diagnosis of HVBCS by CTA technique, but also could noninvasively provide anatomical information and reveal damage to the hepatic parenchyma

关键词： Budd-Chiari syndrome hepatic veno-occlusion disease tomography, X-ray computed angiography

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Enhanced rate capability and cycle stability of Ti2C MXene for sodium storage through an aniline molecules welding strategy

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Tungsten 2025年第1期7卷 161-171页

作者： Hao Chen ya-qin Wang Dong-Ting zhang Bei Zhao Min-Peng Li Chen-yang Li Tian-Peng Xu Mao-Cheng Liu State Key Laboratory of Advanced Processing and Recycling of Non-Ferrous Metals Lanzhou University of Technology School of Materials Science and Engineering Lanzhou University of Technology School of Computer and Communication Technology Lanzhou University of Technology

MXenes obtained significant attention in the field of energy storage devices due to their characteristic layered structure,modifiable surface functional groups,large electrochemically active surface,and regulable interlayer ***,the self-restacking and sluggish ions diffusion kinetics performance of MXenes during the alkali metal ions insertion/extraction process severely impedes their cycle stability and rate *** paper proposes an aniline molecule welding strategy for welding p-phenylenediamine（PPDA） into the interlayers of Ti2C through a dehydration condensation *** welded PPDA molecules can contribute pillar effect to the layered structure of *** pillar effect effectively maintains the structural stability during the sodium ions insertion/extraction process and effectively expands the interlayer spacing of Ti2C from 1.16 to 1.38 nm,thereby enhancing ions diffusion kinetics performance and improving the long-term cycle *** Ti2C-PPDA demonstrates outstanding Na+storage capability,exhibiting a specific capacity of 100.2 mAh·g-1at a current density of 0.1 A·g-1over 960 cycles and delivering a remarkable rate capability 81.2 mAh·g-1at a current density of 5 A·*** study demonstrates that expanding interlayer spacing is a promising strategy to enhance the Na+storage capacity and improve long-term cycling stability,which provides significant guidance for the design of two-dimensional Na+storage materials with high-rate capability and cycle stability.

关键词： Diffusion kinetics Expanding interlayer spacing Rate capability Sodium ions storage Ti2C

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论国家治理体系现代化的公共性价值诉求

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南京师大学报（社会科学版） 2014年第4期 27-34页

作者：张雅勤南京大学政府管理学院武汉科技大学文法与经济学院

现代化的国家治理体系必然包含着价值、制度、组织与机制四种要素。其中,价值赋予了整个治理体系灵魂性根基,决定了治理发展的方向、重心与次序。因此,有必要从价值维度来探求国家治理体系现代化所蕴含的价值诉求。通过历时性回溯分析,... 详细信息

现代化的国家治理体系必然包含着价值、制度、组织与机制四种要素。其中,价值赋予了整个治理体系灵魂性根基,决定了治理发展的方向、重心与次序。因此,有必要从价值维度来探求国家治理体系现代化所蕴含的价值诉求。通过历时性回溯分析,可以看到中西方国家都呈现出"国家统治/管理体系"向"国家治理体系"转型的发展逻辑,而蕴含在这一过程中的价值导向则是一条趋向"公共性"的路径。当前,我国提出"国家治理体系现代化"的战略目标,不仅意味着我国治理制度、方式和手段的现代化变革,更昭示着治理的公共性价值从"形式"走向"实质"的历史进程。就我国实际情况而言,国家治理体系现代化应以价值与技术的统一为方法论前提,以"合作共治"为目标愿景,以社会公正为行动原则,进而谋求平衡价值与技术、权威与自由相统一的现代化治理路径。

关键词：国家治理体系公共性价值形式公共性实质公共性现代化

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一类锥约束凸向量优化问题弱有效解集非空紧性的刻画和应用

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应用数学 2010年第2期23卷 319-324页

作者：张亚琴河北交通职业技术学院土木工程系河北石家庄050091

本文研究当目标空间的控制结构为多面体锥时,锥约束凸向量优化问题的弱有效解集的非空紧性的刻画,然后将所获结果用于研究一类罚函数方法的收敛性.

关键词：向量优化弱有效解锥约束优化罚函数方法

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国家治理现代化的价值导向:“形式公共性”与“实质公共性”相统一

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江苏行政学院学报 2015年第2期 112-119页

作者：张雅勤武汉科技大学文法与经济学院武汉430065

在国家治理的意义上,现代化意味着社会管理机制和文化模式的理性化过程,无疑包含着公共性的内容,但是这种公共性在很大程度上是通过"价值中立"以及技术化、工具化追求营建出的,因而仅仅是一种"形式公共性"。显然,这种"形式公共性"是一... 详细信息

在国家治理的意义上,现代化意味着社会管理机制和文化模式的理性化过程,无疑包含着公共性的内容,但是这种公共性在很大程度上是通过"价值中立"以及技术化、工具化追求营建出的,因而仅仅是一种"形式公共性"。显然,这种"形式公共性"是一种不完整的公共性,缺少公共性的实质内涵,所以当前我国所谋求的"国家治理体系和治理能力现代化",不能仅仅从治理方式和手段等技术化方面照搬西方治理框架进行顶层设计,而应该根据全球化、后工业化时代的要求,以正义的制度为保障探寻国家治理模式从"管理"走向"合作"的实质性转型,从而实现"形式公共性"与"实质公共性"的价值融合,进而在价值与技术、权威与自由的和谐统一下推进国家治理现代化。

关键词：国家治理现代化治理价值形式公共性实质公共性

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苏格兰高尔夫球场设施管理研究

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成都体育学院学报 2006年第1期32卷 35-37,46页

作者：张雅琴深圳大学高尔夫学院广东深圳518060

通过实地调查,研究了苏格兰高尔夫球场设施的管理现状,并结合中国高尔夫球场设施现状和存在的问题进行分析。研究结果表明,苏格兰目前的管理风格仍然是建立在传统的基础之上。研究认为无论是中国还是苏格兰,要想在竞争日趋激烈的市场生... 详细信息

通过实地调查,研究了苏格兰高尔夫球场设施的管理现状,并结合中国高尔夫球场设施现状和存在的问题进行分析。研究结果表明,苏格兰目前的管理风格仍然是建立在传统的基础之上。研究认为无论是中国还是苏格兰,要想在竞争日趋激烈的市场生存,必须采用有效的商业经营原则改造传统的高尔夫球场设施管理系统,并积极参与高尔夫国内外市场竞争,不断寻求新的资源,新的合作伙伴,新的资金和新的经济增长点。

关键词：苏格兰中国高尔夫运动球场设施管理

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学校德育实效性的现实挑战和理论反思

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深圳大学学报（人文社会科学版） 2006年第3期23卷 116-119页

作者：张雅琴深圳大学高尔夫学院广东深圳518060

当前学校德育理论与实践进入了一个全新的历史时期,德育实践面临着时代挑战,如市场化进程中的物化倾向及其带来的精神困惑、教育的功利化趋向、社会价值观多样化、生存方式的虚拟化和数字化等。要提高学校德育实效,德育理论必须进行反... 详细信息

当前学校德育理论与实践进入了一个全新的历史时期,德育实践面临着时代挑战,如市场化进程中的物化倾向及其带来的精神困惑、教育的功利化趋向、社会价值观多样化、生存方式的虚拟化和数字化等。要提高学校德育实效,德育理论必须进行反思和创新。要深化对德育本质的认识,坚持以人为本的德育观;确定高校德育目标,保证和促进学生全面、协调的发展;改进高校德育的方法,推进学校德育的现代化、科学化,以提高学校德育的实效性。

关键词：学校德育实践挑战理论反思

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