Identification of processed Chinese medicinal materials using DNA mini-barcoding

作     者：SONG Ming DONG Gang-Qiang ZHANG Ya-Qin LIU Xia SUN Wei 

作者机构：Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences Beijing 100700 China School of Chemistry Chemical Engineering and Life Sciences Wuhan University of Technology Wuhan 430070 China Amway (China) Botanical Research and Development Center Wuxi 214145 China 

出 版 物：《Chinese Journal of Natural Medicines》 (中国天然药物（英文版）)

年 卷 期：2017年第15卷第7期

页      面：481-486页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by the Major Scientific and Technological Special Project for“Significant New Drugs Creation(No.2014ZX09304307) the Key Projects in he National Science and Technology Pillar Program(No.2011BAI07B08) 

主　　题：DNA barcoding trnL(UAA)intron P6 loop Identification Processed medicinal materials Mini-barcode 

摘      要：Most of Chinese medicinal herbs are subjected to traditional processing procedures, including stir-frying, charring, steaming, boiling, and calcining before they are released into dispensaries. The marketing and identification of processed medicinal materials is a growing issue in the marketplace. However, conventional methods of identification have limitations, while DNA mini-barcoding, based on the sequencing of a short-standardized region, has received considerable attention as a new potential means to identify processed medicinal materials. In the present study, six DNA barcode loci including ITS2, psb A-trn H, rbc L, mat K, trnL(UAA) intron and its P6 loop, were employed for the authentication of 45 processed samples belonging to 15 species. We evaluated the amplification efficiency of each locus. We also examined the identification accuracy of the potential mini-barcode locus, of trnL(UAA) intron P6 loop. Our results showed that the five primary barcode loci were successfully amplified in only 8.89%——20% of the processed samples, while the amplification rates of the trnL(UAA) intron P6 loop were higher, at 75.56% successful amplification. We compared the mini-barcode sequences with Genbank using the Blast program. The analysis showed that 45.23% samples could be identified to genus level, while only one sample could be identified to the species level. We conclude that trnL(UAA) p6 loop is a candidate mini-barcode that has shown its potential and may become a universal mini-barcode as complementary barcode for authenticity testing and will play an important role in medicinal materials control.