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淀粉分子量调控技术研究进展

现代食品科技 2024年第4期40卷 350-362页

作者：张思捷张芯蕊冯志强汪桢王璐阳黄继红河南大学农学院省部共建作物逆境适应与改良国家重点实验室河南开封475004 中原食品实验室河南漯河462300 三全食品股份有限公司河南郑州450001 许昌学院食品与药学院河南许昌461000

天然淀粉是由绿色植物经过光合作用合成的终产物之一,是农作物收获器官的主要储存性多糖之一,也是支撑现代工业发展的重要原料之一。淀粉分子量是研究淀粉分子结构的重要基础参数,也是决定淀粉性质及其应用的重要依据。通过对淀粉分子... 详细信息

天然淀粉是由绿色植物经过光合作用合成的终产物之一,是农作物收获器官的主要储存性多糖之一,也是支撑现代工业发展的重要原料之一。淀粉分子量是研究淀粉分子结构的重要基础参数,也是决定淀粉性质及其应用的重要依据。通过对淀粉分子量进行调控,可以改善天然淀粉的诸多性能,例如热稳定性、凝胶强度、流变学特性等,使其能够更好地应用于医药、化学、食品工业等领域,成为近年来的研究热点。该文从物理、化学、生物技术三个方面对淀粉分子量调控研究进行了总结分析,并探讨了不同调控技术的作用机制及分子量变化对淀粉物化性质和应用产生的影响。提出淀粉分子量调控的新思路,并对未来发展方向进行展望,旨在为研究淀粉分子量及其应用提供理论依据。

关键词：淀粉分子量调控技术性质变化应用

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Towards understanding the correlation between UO_2^(2+) extraction and substitute groups in 2,9-diamide-1,10-phenanthroline

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Science China Chemistry 2018年第10期61卷 1285-1292页

作者： xinrui zhang Liyong Yuan Zhifang Chai Weiqun Shi Laboratory of Nuclear Energy Chemistry Institute of High Energy Physics Chinese Academy of Sciences School for Radiological and Interdisciplinary Sciences (RAD-X) and Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions Soochow University

2,9-Diamide-1,10-phenanthroline(DAPhen)ligands represent a new family of tetradentate extractants given their strong affinity to actinides and the CHON *** this family,N,N′-diethyl-N,N′-ditolyl-2,9-diamide-1,10-phenanthroline(Et-TolDAPhen),initially reported by us,exhibits excellent selectivity towards actinides(U,Th,Am,Pu)over lanthanides and thus can be potentially applied in the group actinide extraction(GANEX)process for the group separation of *** this article,by tailoring the lengths of alkyl chains,we synthesized other four DAPhen ligands with different substitute groups in the diamide moieties,and characterized the relationship between properties and substitute groups of DAPhen *** extraction results show that three of the ligands exhibit high performance in UO_2^(2+)extraction from an acidic solution and the extracted UO_2^(2+)can be easily stripped by only using ultrapure *** titration confirms that UO_2^(2+)combined with all the four ligands in 1:1 *** extended X-ray absorption finestructure(EXAFS)study shows that six donor atoms comprise the first equatorial shell of the UO_2^(2+)ions bonded by the DAPhen ligands,among which two nitrogen and two oxygen atoms are from the DAPhen ligand,while other two oxygen atoms are from one nitrate *** article promises to provide basic data for assessing the feasibility of this kind of DAPhen ligands applied in actinides separation from nuclear wastes.

关键词： 2,9-diamide-1,10-phenanthroline UO2^2+ extraction UV-Vis titration EXAFS

基于双主动全桥双向DC/DC变换器的混合单移相调制方案

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电源学报 2024年第1期22卷 32-40页

作者：李宇张鑫蕊税朝会黄剑琪龙鹏张津华贵州万峰电力股份有限公司兴义562400 国网湖北直流运检公司宜昌443000

对双主动全桥DAB(dual active bridge)双向DC/DC变换器的调制方案进行了研究,DAB变换器的主要优势在于具有对称结构、双向潮流能力、宽软开关区域和灵活的控制能力等特点。控制这种拓扑结构最简单的方法是通过控制变换器原副边全桥之间... 详细信息

对双主动全桥DAB(dual active bridge)双向DC/DC变换器的调制方案进行了研究,DAB变换器的主要优势在于具有对称结构、双向潮流能力、宽软开关区域和灵活的控制能力等特点。控制这种拓扑结构最简单的方法是通过控制变换器原副边全桥之间的移相角来控制功率传输的方向和大小。然而在轻载条件下,当变换器的输入或输出电压变化较大时,会产生大量的无功功率,同时部分开关管的零电压开关ZVS(zero voltage switching)操作会丢失而直接导致转换效率变低。因此,为了提高DAB变换器的效率,提出了一种混合单移相调制PSM(phase shift modulation)方案,在保持控制简单的基础上,通过减小电感电流有效值,扩大软开关的范围,提高了变换器的效率。首先,通过让拓展移相EPS(extended phase shift)、双移相DPS(dual phase shift)以及三移相TPS(triple phase shift)调制方案中的可控变量相等,从而形成了4种不同的PSM方案。接着,对这些调制方案进行了稳态特征的比较分析,包括传输功率容量、电感电流水平以及软开关性能等。在此基础上,提出了一种混合PSM方案。最后,通过搭建实验平台验证了所提出调制方案的有效性和正确性。

关键词：双主动全桥变换器软开关特性电感电流有效值单移相调制方案

Study on enzymatic hydrolysis of soybean β-conglycinin using alkaline protease from Bacillus subtilis ACCC 01746 and antigenicity of its hydrolysates

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Grain & Oil Science and Technology 2021年第1期4卷 18-25页

作者： Haicheng Yin xinrui zhang Jin Huang College of Biological Engineering Henan University of TechnologyZhengzhou 450001China

Due to its beneficial health effects,the use of soybean protein has shown a continuous increase,but concerns regarding the allergenicity of soybean antigenic protein have also *** study aimed to evaluate the hydrolytic effects of a non-commercial alkaline protease isolated from the Bacillus subtilis ACCC 01746 on soybeanβ-conglycinin and the allergenicity of its *** protease of the strain was separated by precipitation method of organic solvents,and theβ-conglycinin was separated by alkali-solution and acid-isolation and purified by use of gel *** the degree of hydrolysis(DH)and inhibition rate as evaluation indexes,the enzymatic hydrolysis parameters ofβ-conglycinin was optimized by single factor and L_(9)(3^(4))orthogonal tests,so as to explore the effect of the protease on the hydrolysis degree and the antigenicity ofβ-conglycinin *** results showed that the native enzyme existed as an 18.3 kDa monomer with a 430 U/g maximum *** purity ofβ-conglycinin was 84.8%.The single-factor test results showed that DH showed the oppostie trendency with the inhibition rate,and the increase of protein concentration causedmonotone increasing and monotone decreasing of the inhibition rate and the DH,and the optimal protein concentration was 30 mg/*** optimization results showed that pH had the largest impacts on both DH and the inhibition rate,followed by enzyme dosage,hydrolysis temperature and hydrolysis *** the optimum hydrolysis conditions of protein concentration 30mg/mL,enzymedosage0.7%,hydrolysis time40min,temperature 55°C and pH8.5,the DH reached the highest of 76.28%,and the inhibition rate was the lowest of 27.03%,which was reduced greatly compared with that before *** results suggested that alkaline protease appeared to show a relatively high effeciency in lowering soybean allergenicity,making it possible to produce low-allergenicity soybean protein.

关键词： Alkaline protease Bacillus subtilis ACCC 01746 β-conglycinin Hydrolysis degree Antigenicity

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基于测试代价的三支邻域属性约简算法

计算机应用研究 2024年第3期41卷 836-841页

作者：张欣蕊万仁霞岳晓冬陈瑞典北方民族大学数学与信息科学学院银川750021 上海大学计算机工程与科学学院上海200444 福建弘扬软件股份有限公司健康大数据研究院福州350002

针对粗糙集属性约简时很少考虑属性自身的测试代价等问题,提出了一种基于测试代价的三支邻域属性约简算法。算法根据各属性在邻域分辨矩阵中出现的频次和比例来计算属性重要性,并结合属性自身的测试代价来构造性价比指标,以此指导属性... 详细信息

针对粗糙集属性约简时很少考虑属性自身的测试代价等问题,提出了一种基于测试代价的三支邻域属性约简算法。算法根据各属性在邻域分辨矩阵中出现的频次和比例来计算属性重要性,并结合属性自身的测试代价来构造性价比指标,以此指导属性的甄选。三支决策方法被用于划分属性集,为属性的约简处理提供数据支撑。在7个UCI公共数据集上进行对比实验,结果表明,该算法可得到比对比算法更小的属性约简集合,在分类精度不降低的情况下,该算法具有更少的运行时间和更小的测试代价。基于财政收入的预测应用实例进一步证明了所提算法的有效性和实用性。

关键词：邻域粗糙集邻域分辨矩阵属性约简测试代价三支决策

大鼠脂肪间充质干细胞及其外泌体的蛋白组学对比分析

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中国组织工程研究 2025年第13期29卷 2683-2689页

作者：张鑫瑞韩悦雷蕾刘建宇耿承奎云南中医药大学第二临床医学院云南中医药大学呈贡校区云南省昆明市650000 昆明医科大学附属延安医院骨科云南省昆明市650000

背景:从蛋白组学方面分析脂肪间充质干细胞及其来源外泌体促进组织修复的研究较多,但缺少二者之间的蛋白组学对比分析。目的:对脂肪间充质干细胞及其外泌体进行蛋白组学分析。方法:分离培养大鼠脂肪间充质干细胞,从细胞上清液中提取外... 详细信息

背景:从蛋白组学方面分析脂肪间充质干细胞及其来源外泌体促进组织修复的研究较多,但缺少二者之间的蛋白组学对比分析。目的:对脂肪间充质干细胞及其外泌体进行蛋白组学分析。方法:分离培养大鼠脂肪间充质干细胞,从细胞上清液中提取外泌体并进行鉴定;运用DIA蛋白组学分析大鼠脂肪间充质干细胞及其外泌体差异表达蛋白,对差异表达蛋白进行GO和KEGG分析。结果与结论:①脂肪间充质干细胞以长梭形为主,类似成纤维样;②经BCA法测定外泌体悬液的蛋白质量浓度为7.66 mg/mL;③外泌体呈典型的茶杯型,可见双层膜的囊泡结构,中央为低电子密度成分,外泌体粒径分布峰值为112.2 nm,浓度为7.5×10^(11)粒子/mL,平均直径在70-140 nm之间;④外泌体有表面标记蛋白CD9、TSG101高表达;⑤GO分析脂肪间充质干细胞及其外泌体的差异表达蛋白主要集中在细胞外基质和突触等位置,与离子结合和核糖体的结合能力相关,在细胞黏附和翻译过程中最为显著;⑥KEGG通路分析结果显示,差异表达蛋白主要参与细胞外基质受体交互、核糖体和细胞因子受体相互作用等,并且与胆固醇和甲状腺等多种代谢性疾病有关。

关键词：脂肪间充质干细胞外泌体蛋白组学对比分析组织修复

Growth of Single-walled Carbon Nanotubes on Substrates Using Carbon Monoxide as Carbon Source

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Chemical Research in Chinese Universities 2021年第5期37卷 1125-1129页

作者： ZHAO Xue zhang xinrui LIU Qidong zhang Zeyao LI Yan Beijing National Laboratory for Molecular Science Key Laboratory for the Physics and Chemistry of NanodevicesState Key Laboratory of Rare Earth Materials Chemistry and ApplicationsCollege of Chemistry and Molecular EngineeringPeking UniversityBeijing100871P.R.China Peking University Shenzhen Institute Shenzhen518057P.R.China College of Engineering Peking UniversityBeijing100871P.R.China PKU-HKUST ShenZhen-HongKong Institution Shenzhen518057P.R.China

The growth of single-walled carbon nanotubes(SWCNTs)on substrates has attracted great interests because of the potential applications in various *** monoxide(CO)was used as the carbon source for the growth of SWCNTs on silicon *** or oriented SWCNTs can be produced by varying the CO flow *** the flow rate of CO was as low as 20 sccm(sccm:standard cubic centimeter per minute).

关键词： SWCNTs Carbon walled

Enzymatic kinetics ofβ-conglycinin using alkaline protease from Bacillus subtilis ACCC 01746 and analysis of antigenicity of hydrolyzed peptide

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Grain & Oil Science and Technology 2021年第3期4卷 141-148页

作者： Haicheng Yin xinrui zhang Zhixiang Yang Jin Huang College of Biological Engineering Henan University of TechnologyZhengzhou 450001China

β-Conglycinin,the main protein of soybean,is a key allergen that causes soybean allergies,and hydrolysis is usually applied to lower its *** evaluated the enzymolysis characters ofβ-conglycinin from the perspective of enzymolysis kinetics using alkaline protease from *** ACCC 01746.A dynamic model describing the hydrolysis ofβ-conglycinin was proposed using the initial substrate concentration,enzyme dosage(enzyme to substrate ratio)and hydrolysis time as variables to illustrate the kinetic behavior of enzymatic *** hydrolysis of soybeanβ-conglycinin was carried out at 60 g/L protein concentration,0.6%enzyme dosage,55℃ and pH 8.5 to observe the peptides with anti-enzymatic *** hydrolysates were gradually fractionated by ultrafiltration through cut-off membranes with molecular weights of 40,30,20,and 10 kDa,and their antigenicities were evaluated using indirect competitive enzyme-linked immunosorbent *** results showed that the degree of hydrolysis(DH)ofβ-conglycinin decreased as theβ-conglycinin concentration(S0)increased,but increased with enzyme dosage(E0)***,the enzymatic hydrolysis ofβ-conglycinin followed the first-order kinetics *** hydrolysis rate(V)was(527.89C_(E0)-2.5533C_(S0))exp(-0.022DH),the DH-hydrolysis time was 45.454ln[1+(11.614C_(E0)/C_(S0)-0.0562)t],and the correlated kinetic constants k2 and kd were 527.89 min^(−1)and 8.6126 min^(−1),*** hydrolysis behavior ofβ-conglycinin varied considerably among theα',α,andβ*** hydrolysis rates were observed for theα'andαsubunits compared to theβ*** relative molecular weights of the intercepted peptides from the hydrolysates were 14.8-40.1 kDa,and the antigenicity of the peptides with smaller molecular weight was reduced,but not removed ***,the alkaline protease from the strain appeared to effectively reduce the allergenicity ofβ-***,it is possible to produce less allergenic soybean

关键词： B.subtilis ACCC 01746 Alkaline protease β-Conglycinin Enzymatic kinetics Antigenicity

妊娠诊断方法及再次人工授精方案对奶牛繁殖效率的影响研究进展

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中国畜牧杂志 2024年第9期60卷 43-47页

作者：孙国瀚张馨蕊郑浩周佳敏陶金忠宁夏大学动物科技学院宁夏银川750021

奶牛养殖业发展是我国畜牧业规模化进程的重要部分,近年来,我国奶牛养殖业设施规模持续扩大,产奶量逐年升高,但繁殖效率低导致的奶牛总体产量不足等问题仍限制产业发展。随着繁殖技术的进步,通过合理的妊娠诊断方法对奶牛妊娠状态进行... 详细信息

奶牛养殖业发展是我国畜牧业规模化进程的重要部分,近年来,我国奶牛养殖业设施规模持续扩大,产奶量逐年升高,但繁殖效率低导致的奶牛总体产量不足等问题仍限制产业发展。随着繁殖技术的进步,通过合理的妊娠诊断方法对奶牛妊娠状态进行检测并结合再次人工授精方案,可以有效降低奶牛的胚胎损失率,提高奶牛的妊娠率,进而有效提高奶牛繁殖效率,创造更多经济效益。本文总结了奶牛常用的妊娠诊断方法及再次人工授精方案对奶牛后续繁殖效率的影响,以期为奶牛繁殖技术的应用和繁殖效率的提高提供理论参考。

关键词：妊娠诊断人工授精奶牛繁殖效率