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Synthesis of Coumarin-functionalized Calix[4]arene and Selective Recognition for L-Tryptophan

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Chinese Chemical Letters 2006年第5期17卷 575-577页

作者： Jian quan LU li zhang Ting quan SUN Gui Xiang WANG ling Yan WU Hubei Key Laboratory of Bioanalytical Technique Hubei Normal University Huangshi 435002

5,11,17,23-Tetra-tert-butyl-25, 27-bis（7＇-methoxycoumarin-3＇-methyloxy）-26, 28-dihydroxycalix[4]arene was synthesized and characterized. The derivative could recognize L-tryptophan with a 1：1 complexing ratio and an apparent equilibrium constant of 111 L mol^-1 in DMF acetonitrile as solvent by UV-visible and fluorescent spectrophotometry.

关键词： Calixarene synthesis recognition L-tryptophan.

Crystal Structure and Theoretical Calculation of N-[4-(2-Methoxyphenoxy)]phenyl Acetanilide

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Chinese Journal of Structural Chemistry 2010年第7期29卷 1077-1083页

作者： zhang Cheng-Gang li quan WANG Bi-Qin ZHAO Ke-Qing College of Chemistry and Material Science Sichuan Normal University Key Laboratory of Advanced Functional Materials Sichuan Higher Education System Chengdu 610066 China

N-[4-（2-Methoxyphenoxy）]phenyl acetanilide has been synthesized and its structure was determined by X-ray diffraction analysis with a = 0.6152（4）, b = 1.1728（4）, c = 1.2367（4） nm, α = 91.34（3）, β = 93.22（4）, γ = 102.61（3）°, V = 0.8688（7） nm3 and Dc = 1.274 Mg/m3. Theoretical investigations on the title compound were carried out by full optimization on its structural unit at the B3LYP/6-31G＊＊ level. And ultimately, the infrared, Raman spectra, electronic absorption spectra, and the relationships between temperature and thermodynamic functions （Hm, Sin, Cpm） were obtained.

关键词： phenyl acetanilide crystal structure spectra thermodynamic properties

Embryo-chondrocyte expressed gene 1,downregulating hypoxiainducible factor 1α,is another marker of lung tumor hypoxia

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Acta Pharmacologica Sinica 2007年第4期28卷 549-558页

作者： li zhang Qing-quan li Department of Respiratory Disease,Renmin Hospital,Wuhan University,Wuhan 430060,China Department of Respiratory Disease Renmin Hospital Wuhan University Wuhan China

Aim:To explore the mechanism of differentiated embryo-chondrocyte expressedgene 1 (DEC 1) in the lung adenocarcinoma A549 cell line and its relationship withhypoxia-inducible factor 1α(HIF-1α).Methods:DEC 1 and HIF-1α protein levelswere detected in lung adenocarcinoma tissues by immunohistochemistry.A549cells were cultured at *** assay was used to determine the effect of *** apoptotic analysis was determined by flow ***-PCR andimmunocytochemistry were carried out to observe the DEC 1 and HIF-1α mRNAand protein ***-1α mRNA and protein levels were detected in thestably transfected pcDNA-DEC 1+/-A549 cells by RT-PCR and Western ***1 mRNA and protein levels were detected in the stably transfected pcDNA-DEC 1+/-A549 cells with or without H1F-1α antisense oligonucleotide treatmentby RT-PCR and Western ***:DEC1 was specifically located in 40(48.8%) lung adenocarcinoma *** results of the MTT test showed thegrowth of the stably transfected pcDNA-DEC+ A549 cells was higher than thoseof A549 cells and the stably transfected pcDNA-DEC-A549 *** alsoinduced the apoptosis of A549 cells and the stably transfected pcDNA-DEC+/-A549 *** increased the mRNA and protein levels of DEC1 and HIF-1α.Both HIF-1α mRNA and protein levels decreased in the stably transfected pcDNA-DEC+cells,but HIF-1α antisense oligonucleotide had no effect on DEC1 in thestably transfected pcDNA-DEC+/-A549 ***:DEC 1 is a marker oftumor ***1 downregulates the HIF-1α at both mRNA and protein levelsat hypoxia in lung adenocarcinoma A549 cells.

关键词： DECI lung adenocarcinoma hypoxia HIF-1

An Adaptive Learning Method for the Generation of Fuzzy Inference System from Data

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自动化学报 2008年第1期34卷 80-87页

作者： zhang li-quan SHAO Cheng Institute of Advanced Control Technology Dalian University of Technology Dalian 116024 P.R. China College of Electric and Information Engineering Beijing University of Civil Engineering and Architecture Beijing 100044 P.R. China

Designing a fuzzy inference system(FIS)from data can be divided into two main phases:structure identification and parameter ***,starting from a simple initial topology,the membership functions and system rules are defined as specific ***,to speed up the convergence of the learning algorithm and lighten the oscillation,an improved descent method for FIS generation is ***, the convergence and the oscillation of the algorithm are system- atically ***,using the information obtained from the previous phase,it can be decided in which region of the in- put space the density of fuzzy rules should be enhanced and for which variable the number of fuzzy sets that used to partition the domain must be ***,this produces a new and more appropriate ***,the proposed method is applied to the problem of nonlinear function approximation.

关键词：自适应学习模糊推论系统数据处理非线性函数逼近梯度演化信度

Identification of Differentially Expressed Genes During Ethylene Climacteric of Melon Fruit by Suppression Subtractive Hybridization

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Journal of Integrative Agriculture 2013年第8期12卷 1431-1440页

作者： GAO Feng NIU Yi-ding HAO Jin-feng BADE Rengui zhang li-quan HASIAgula Inner Mongolia Key Laboratory of Herbage & Endemic Crop Biotechnology/College of life Sciences Inner Mongolia University

Melon （Cucumis melo L.） is an important horticultural crop worldwide. Ethylene regulates the ripening process and affects the ripening rate. To screen genes that are differentially expressed at the burst of ethylene climacteric in melon fruit, we performed suppression subtractive hybridization （SSH） to generate forward and reverse libraries, for which we sequenced 439 and 445 clones, respectively. Our BLAST analysis showed that the genes from the 2 libraries were involved in metabolism, signal transduction, cell structure, transcription, translation, and defense. Six genes were analyzed by qRT-PCR during the differential developmental stage of melon fruit. Our results provide new insight into the understanding of climacteric ripening of melon fruit.

关键词： Cucumis melo ethylene climacteric fruit ripening differentially expressed mRNA gene expression suppressionsubtractive hybridization

A maize bundle sheath defective mutation mapped on chromosome 1 between SSR markers umc1395 and umc1603

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Journal of Integrative Agriculture 2015年第10期14卷 1949-1957页

作者： PAN Yu CHEN Xu-qing XIE Hua DENG Lei li Xiang-long zhang Xiao-dong HAN li-xin YANG Feng-ping XUE Jing zhang li-quan Beijing Agro-Biotechnology Research Center Beijing Academy of Agricultural and Forestry Science Key Laboratory of Horticulture Science for Southern Mountainous Regions Ministry of Education/College of Horticulture and Landscape Architecture Southwest University Bioengineering College Chongqing University

The bsd-pg（bundle sheath defective pale green） mutant is a novel maize mutation, controlled by a single recessive gene, which was isolated from offspring of maize plantlets regenerated from tissue callus of the maize inbred line 501. The characterization was that the biogenesis and development of the chloroplasts was mainly interfered in bundle sheath cells rather than in mesophyll cells. For mapping the bsd-pg, an F2 population was derived from a cross between the mutant bsd-pg and an inbred line Xianzao 17. Using specific locus amplified fragment sequencing（SLAF-Seq） technology, a total of 5 783 polymorphic SLAFs were analysed with 1 771 homozygous alleles between maternal and paternal parents. There were 49 SLAFs, which had a ratio of paternal to maternal alleles of 2：1 in bulked normal lines, and three trait-related candidate regions were obtained on chromosome 1 with a size of 3.945 Mb. For the fine mapping, new simple sequence repeats（SSRs） markers were designed by utilizing information of the B73 genome and the candidate regions were localized a size of 850 934 bp on chromosome 1 between umc1603 and umc1395, including 35 candidate genes. These results provide a foundation for the cloning of bsd-pg by map-based strategy, which is essential for revealing the functional differentiation and coordination of the two cell types, and helps to elucidate a comprehensive understanding of the C4 photosynthesis pathway and related processes in maize leaves.

关键词： maize bsd-pg SLAF SSR assossiation analysis fine mapping

Martensitic Transformation and Magnetic-Field-Induced Strain in Magnetic Shape Memory Alloy NiMnGa Melt-Spun Ribbon

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Journal of Materials Science & Technology 2005年第2期21卷 211-214页

作者： Shihai GUO, Yanghuan zhang, Jianliang li, Baiyun quan, Yan QI and Xinlin WANG Department of Functional Material Research, Central Iron and Steel Research Institute, Beijing 100081, China Dept. of Functional Mat. Res. Central Iron and Steel Res. Inst. Beijing 100081 China

A magnetic shape memory alloy with nonstoichiometric Ni50Mn27Ga23 was prepared by using melt-spinning technology. The martensitic transformation and the magnetic-field-induced strain (MFIS) of the polycrystalline melt-spun ribbon were investigated. The experimental results showed that the melt-spun ribbons underwent thermal-elastic martensitic transformation and reverse transformation in cooling and heating process and exhibited typical thermo-elastic shape memory effect. However the start temperature for martensitic transformation decreased from 286 K for as-cast alloy to 254 K for as-quenched ribbon and Curie temperature remains approximately constant. A particular internal stress induced by melt-spinning resulted in the formation of a texture structure in the ribbons, which made the ribbons obtain larger martensitic transformation strain and MFIS. The internal stress was released substantially after annealing, which resulted in a decrease of MFIS of the ribbons.

关键词： Shape memory alloy Ferromagnetism Melt-spinning Martensitic transformation Magnetic-field-induced strain

Primary Reconstruction of Completely Biologic Tissue Engineered Blood Vessel and Related Basic Research

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生物医学工程学杂志 2005年第S1期22卷 51-52页

作者： Xiu-Hua zhang Yan zhang Yu-quan li Xiang-Qun YANGlin zhang Hui-Long HUANG(Institute of Biomedical Engineering, The Second Military Medical University, Shanghai 200433,China)

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基于完备模糊规则集的混沌时间序列预测

系统仿真学报 2006年第6期18卷 1593-1596,1600页

作者：张立权邵诚大连理工大学信息与控制研究中心大连116024

基于数据挖掘思想,使用置信度度量和改进的梯度下降法,提出一种新的构造完备模糊规则集的方法来建模和预测混沌时间序列。所提方法通过确定最优输出模糊子集的质心和模糊规则的置信度度量,能够推理数据未覆盖区域的空缺规则,并构造一个... 详细信息

基于数据挖掘思想,使用置信度度量和改进的梯度下降法,提出一种新的构造完备模糊规则集的方法来建模和预测混沌时间序列。所提方法通过确定最优输出模糊子集的质心和模糊规则的置信度度量,能够推理数据未覆盖区域的空缺规则,并构造一个完备的模糊规则集,进而解决了混沌时间序列的可预测问题。仿真结果表明新方法是有效和准确的。它能很好地辨识系统的特征,并且提供了一种混沌时间序列预测的新方法。

关键词：完备模糊规则集置信度混沌梯度下降法