Embryo-chondrocyte expressed gene 1,downregulating hypoxiainducible factor 1α,is another marker of lung tumor hypoxia
Embryo-chondrocyte expressed gene 1,downregulating hypoxiainducible factor 1α,is another marker of lung tumor hypoxia作者机构：Department of Respiratory Disease Renmin Hospital Wuhan University Wuhan China
出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))
年 卷 期：2007年第28卷第4期
页 面：549-558页
核心收录：
学科分类：1007[医学-药学(可授医学、理学学位)] 1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：DECI lung adenocarcinoma hypoxia HIF-1
摘 要：Aim:To explore the mechanism of differentiated embryo-chondrocyte expressedgene 1 (DEC 1) in the lung adenocarcinoma A549 cell line and its relationship withhypoxia-inducible factor 1α(HIF-1α).Methods:DEC 1 and HIF-1α protein levelswere detected in lung adenocarcinoma tissues by immunohistochemistry.A549cells were cultured at *** assay was used to determine the effect of *** apoptotic analysis was determined by flow ***-PCR andimmunocytochemistry were carried out to observe the DEC 1 and HIF-1α mRNAand protein ***-1α mRNA and protein levels were detected in thestably transfected pcDNA-DEC 1+/-A549 cells by RT-PCR and Western ***1 mRNA and protein levels were detected in the stably transfected pcDNA-DEC 1+/-A549 cells with or without H1F-1α antisense oligonucleotide treatmentby RT-PCR and Western ***:DEC1 was specifically located in 40(48.8%) lung adenocarcinoma *** results of the MTT test showed thegrowth of the stably transfected pcDNA-DEC+ A549 cells was higher than thoseof A549 cells and the stably transfected pcDNA-DEC-A549 *** alsoinduced the apoptosis of A549 cells and the stably transfected pcDNA-DEC+/-A549 *** increased the mRNA and protein levels of DEC1 and HIF-1α.Both HIF-1α mRNA and protein levels decreased in the stably transfected pcDNA-DEC+cells,but HIF-1α antisense oligonucleotide had no effect on DEC1 in thestably transfected pcDNA-DEC+/-A549 ***:DEC 1 is a marker oftumor ***1 downregulates the HIF-1α at both mRNA and protein levelsat hypoxia in lung adenocarcinoma A549 cells.
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