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Transition of One Mode-Phase at the Crisis and Onset of Spatiotemporal Chaos

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Chinese Physics Letters 2001年第2期18卷 178-180页

作者： HE Kai-Fen zhang hai-yun Key Laboratory in University for Radiation Beam Technology and Materials Modification Beijing Normal UniversityBeijing 100875 Institute of Low Energy Nuclear Physics Beijing Normal UniversityBeijing 100875 Beijing Radiation Center Beijing 100875

In our previous work[***.80(1998)696]we investigated a crisis-induced transition from temporal chaos to spatiotemporal chaos(STC)due to a collision between the unstable orbit of the carrier saddle steady wave(SSW)and the attractor of its perturbation wave(PW).In this letter,we find that at the crisis one PW mode-phase experiences a state *** variations of PW mode-phases are governed by the linear dispersion as well as two competitive effects arising from the system nonlinearity:(1)interaction between the SSW and the PW(SP);(2)self-nonlinearity of the PW(PP).With the increasing importance of system nonlinearity we find that before the crisis for all PW modes the SP effects dominate and the mode-phases vibrate chaotically;while after the crisis for one PW mode the PP effect surpasses that of SP,in which case the phase transits to a state of combination of chaotic whirling and vibrating,which is different from the case of weak *** the meantime,the PW partial wave is free from the trapping of its carrier,and can be driven or damped chaotically depending on the phase difference relative to the carrier SSW,causing the STC motion after the crisis.

关键词： nonlinearity chaotic chaos

复合营养补充剂对犬猫采食性能、血液指标、免疫性能及抗氧化功能的影响

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饲料研究 2024年第1期47卷 137-142页

作者：张云海刘公言胡婉静吕悦任启峰孙海涛崔凯刘策山东省农业科学院畜牧兽医研究所济南市宠物科技重点实验室山东济南2501001 青岛农业大学动物科技学院山东青岛2661092 广州麦宝生物科技有限公司广东广州510000

试验旨在研究复合营养补充剂对犬猫免疫性能及抗氧化功能的影响。选取20只成年健康临清狮猫和20只成年健康中华田园犬,各随机分为对照组和试验组,每组10个重复。试验组将5 g复合营养补充剂与60 mL饮水混合补饲,对照组不进行补饲。正式... 详细信息

试验旨在研究复合营养补充剂对犬猫免疫性能及抗氧化功能的影响。选取20只成年健康临清狮猫和20只成年健康中华田园犬,各随机分为对照组和试验组,每组10个重复。试验组将5 g复合营养补充剂与60 mL饮水混合补饲,对照组不进行补饲。正式试验期30 d。结果显示,与对照组相比,试验组犬猫末重、平均日采食量无显著差异(P>0.05),试验组犬平均日饮水量显著降低(P<0.05),试验组犬猫平均水分总摄入量均显著提高(P<0.05)。与对照组相比,试验组猫血液平均血红蛋白含量、平均红细胞体积以及球蛋白含量显著提高(P<0.05),试验组犬血液粒细胞数显著提高(P<0.05);试验组犬猫血清超氧化物歧化酶(SOD)活性以及白细胞介素-6(IL-6)含量均显著提高(P<0.05);试验组猫血清免疫球蛋白D(IgD)含量显著提高(P<0.05),试验组犬血清免疫球蛋白A(IgA)以及免疫球蛋白G(IgG)含量显著提高(P<0.05);试验组犬血清丙二醛含量显著降低(P<0.05)。试验组猫粪便评分显著高于对照组(P<0.05)。研究表明,复合营养补充剂能够促进犬猫饮水,提高犬猫机体的抗氧化能力和免疫力。

关键词：复合营养补充剂犬猫血液指标免疫性能抗氧化功能

Carbon and Nitrogen Storage in Inner Mongolian Grasslands: Relationships with Climate and Soil Texture

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Pedosphere 2014年第3期24卷 391-398页

作者： HE Nian-Peng WANG Ruo-Meng zhang yun-hai CHEN Quan-Sheng Key Laboratory of Ecosystem Network Observation and Modeling Institute of Geographic Sciences and Natural Resources Research Chinese Academy of Sciences State Key Laboratory of Vegetation and Environmental Change Institute of Botany Chinese Academy of Sciences

Understanding the spatial variability of soil carbon （C） storage and its relationship with climate and soil texture is critical for developing regional C models and for predicting the potential impact of climate change on soil C storage. On the basis of soil data from a transect across the Inner Mongolian grasslands, we determined the quantitative relationships of C and nitrogen （N） in bulk soil and particle-size fractions （sand, silt, and clay） with climate and soil texture to evaluate the major factors controlling soil C and N storage and to predict the effect of climate changes on soil C and N storage. The contents of C and N in the bulk soil and the different fractions in the 0 20 and 20 40 cm soil layers were positively correlated with the mean annum precipitation （MAP） and negatively correlated with the mean annual temperature （MAT）. The responses of C storage in the soil and particle-size fractions to MAP and MAT were more sensitive in the 0-20 cm than in the 2（~40 cm soil layer. Although MAP and MAT were both important factors influencing soil C storage, the models that include only MAP could well explain the variation in soil C storage in the Inner Mongolian grasslands. Because of the high correlation between MAP and MAT in the region, the models including MAT did not significantly enhance the model precision. Moreover, the contribution of the fine fraction （silt and clay） to the variation in soil C storage was rather small because of the very low fine fraction content in the Inner Mongolian grasslands.

关键词： mean annual precipitation mean annual temperature native grassland particle-size fraction soil organic matter

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苯并[a]芘污染土壤修复研究进展

土壤通报 2024年第2期55卷 584-596页

作者：吕卫光陈昭良张娟琴张翰林李双喜郑宪清张海韵张月白娜玲上海市农业科学院生态环境保护研究所上海201403 上海应用技术大学生态技术与工程学院上海201418 农业农村部上海农业环境与耕地保育科学观测试验站上海201403 上海市农业环境保护监测站上海201403 农业农村部东南沿海农业绿色低碳重点实验室上海201403 上海市设施园艺技术重点实验室上海201403

苯并[a]芘(Bap)是一种由5个苯环组成的分布广泛、广受关注、“三致”毒性极强的多环芳烃,也是主要的环境污染物监测对象。Bap在土壤等环境中长期积累,可通过食物链的累积和放大效应威胁人体健康。Bap污染土壤的高效修复迫在眉睫,本文综... 详细信息

苯并[a]芘(Bap)是一种由5个苯环组成的分布广泛、广受关注、“三致”毒性极强的多环芳烃,也是主要的环境污染物监测对象。Bap在土壤等环境中长期积累,可通过食物链的累积和放大效应威胁人体健康。Bap污染土壤的高效修复迫在眉睫,本文综述了Bap的产生、污染现状与毒性效应、不同修复技术的优缺点。物理/化学方法具有修复周期短、范围广,但成本高且易产生二次污染等特点;生物修复成本低、操作简单、环境效益好,但修复周期长、效果受多因素影响。在实际治理过程中应综合考虑成本和效果,重点关注微生物修复及相关联合修复技术的应用,将为今后开展Bap类有机污染土壤的绿色修复提供科学思路。

关键词：苯并[a]芘污染土壤修复修复技术联合应用

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现代生物医学进展 2024年第13期24卷 2521-2526页

作者：李尚真崔云云张海云冀乃喜刘文浩西宁市第二人民医院重症医学科青海西宁810000 西宁市第二人民医院消化内科青海西宁810000

目的:探究急诊腹腔感染相关脓毒症患者血清脑钠肽(BNP)、降钙素原(PCT)、白介素-1受体相关蛋白(SIGIRR)变化及与预后的关系。方法:将我院于2020年1月-2023年10月接收的318例急诊腹腔感染患者,根据是否发生脓毒症,将其分为单纯感染组(n=2... 详细信息

目的:探究急诊腹腔感染相关脓毒症患者血清脑钠肽(BNP)、降钙素原(PCT)、白介素-1受体相关蛋白(SIGIRR)变化及与预后的关系。方法:将我院于2020年1月-2023年10月接收的318例急诊腹腔感染患者,根据是否发生脓毒症,将其分为单纯感染组(n=233)与脓毒症组(n=85),对比两组患者基线资料、血清BNP、PCT、SIGIRR水平差异,采用Pearson相关分析法探究血清各指标与临床资料相关性。将脓毒症组患者根据其28d内生存情况,分为生存组(n=56)与死亡组(n=29),对比两组患者基线资料、血清BNP、PCT、SIGIRR水平差异,采用二元logistics方程分析影响脓毒症患者预后的相关影响因素;采用ROC曲线分析血清BNP、PCT、SIGIRR水平对脓毒症组预后的诊断价值。结果:单纯感染组与脓毒症组患者病原体种类、WBC、CRP、ALB、APACHEⅡ及SOFA比较差异显著(P<0.05);单纯感染组血清BNP、PCT、SIGIRR水平均显著低于脓毒症组(P<0.05);Pearson相关性分析显示,脓毒症组患者BNP、PCT、SIGIRR水平与WBC、CRP、APACHEⅡ评分、SOFA评分均呈显著正相关(P<0.05),与ALB呈显著负相关(P<0.05);脓毒症患者中,死亡组血清BNP、PCT、SIGIRR水平均显著高于生存组(P<0.05);二元logistics回归分析显示,血清BNP、PCT及SIGIRR水平均与脓毒症患者不良预后相关(P<0.05);ROC曲线显示,血清BNP、PCT、SIGIRR单独及联合预测脓毒症患者不良预后的AUC分别为0.876、0.826、0.779、0.976,敏感度为86.21%、82.76%、75.86%、96.55%,特异度为75%、75%、71.43%、69.64%,联合诊断价值更高。结论:BNP、PCT、SIGIRR与腹腔感染相关脓毒症患者具有一定关联性,可作为临床诊治及预后评估参考指标。

关键词：腹腔感染脓毒症心肌损伤脑钠肽降钙素原

MicroRNA-370-5p inhibits pigmentation and cell proliferation by downregulating mitogen-activated protein kinase kinase kinase 8 expression in sheep melanocytes

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Journal of Integrative Agriculture 2023年第4期22卷 1131-1141页

作者： JI Kai-yuan WEN Ru-jun WANG Zheng-zhou TIAN Qian-qian zhang Wei zhang yun-hai Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control College of Animal Science and TechnologyAnhui Agricultural UniversityHefei 230036P.R.China Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resources Conservation and Bio-Breeding College of Animal Science and TechnologyAnhui Agricultural UniversityHefei 230036P.R.China Linquan Comprehensive Experimental Station Anhui Agricultural UniversityLinquan 236400P.R.China

In mammals,microRNAs(miRNAs)play key roles in multiple biological processes by regulating the expression of target *** have found that the levels of miR-370-5p expression differ significantly in the skins of sheep with different hair colors;however,its function remains *** this study,we investigated the roles of miR-370-5p in sheep melanocytes and found that the overexpression of miR-370-5p significantly inhibited cell proliferation(P<0.01),tyrosinase activity(P=0.001)and significantly reduced(P<0.001)melanin *** prediction revealed that the 3′-untranslated region(UTR)of MAP3K8 has a putative miR-370-5p binding site,and the interaction between these two molecules was confirmed using luciferase reporter *** situ hybridization assays revealed that MAP3K8 is expressed in the cytoplasm of *** results of quantitative RT-PCR and Western blotting analyses revealed that overexpression of miR-370-5p in melanocytes significantly inhibits(P<0.01)MAP3K8 expression via direct targeting of its 3′*** of MAP3K8 expression by siRNA-MAP3K8 transfection induced a significant inhibition(P<0.01)of melanocyte proliferation and significant reduction(P<0.001)in melanin production,which is consistent with our observations for *** gene rescue experiments indicated that the expression of MAP3K8 in melanocytes co-transfected with miR-370-5p and MAP3K8-cDNA(containing sites for the targeted binding to miR-370-5p)was significantly rescued(P≤0.001),which subsequently promoted significant increases in cell proliferation(P<0.001)and melanin production(P<0.01).Collectively,these findings indicate that miR-370-5p plays a functional role in inhibiting sheep melanocyte proliferation and melanogenesis by downregulating the expression of MAP3K8.

关键词： microRNA mitogen-activated protein kinase kinase kinase 8 melanogenesis sheep melanocytes cell proliferation

Four-mode coherent-entangled state and its application

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Chinese Physics B 2012年第1期21卷 79-86页

作者： Li Hong-Qi Ren Ting-Qi zhang yun-hai Xu Xing-Lei Department of Physics Qufu Normal UniversityQufu 273165China Department of Physics Heze UniversityHeze 274015China Key Laboratory of Quantum Communication and Calculation Heze UniversityHeze 274015China

A new kind of four-mode continuous variable coherent-entangled state is proposed in the Fock space by using the technique of integration within an ordered product, which exhibits both the properties of a coherent state and an entangled state, and spans a complete and orthonormal representation. The conjugate state of the four-mode continuous variable coherent-entangled state is derived by using the Fourier transformation. Moreover, a simple experimental protocol of generating a four-mode continuous variable coherent-entangled state is proposed by using beam splitters. As applications of this four-mode continuous variable coherent-entangled state, a four-mode entangled state and a four-mode squeezing-Fresnel operator are constructed.

关键词： coherent-entangled state beam splitter squeezing-Fresnel operator technique of inte-gration within an ordered product

安徽白山羊SOCS3基因克隆、表达分析及其功能鉴定

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农业生物技术学报 2024年第9期32卷 2071-2080页

作者：崔恒源马存霞崔双双郭玉柱刘洪瑜张运海宋宁安徽农业大学动物科技学院安徽省地方畜禽遗传资源保护与生物育种重点实验室

细胞因子信号转导抑制因子3 (suppressor of cytokine signaling 3, SOCS3)是一种对信号传导作出应答的蛋白，具有调控乳蛋白合成的作用。αS1-酪蛋白是奶畜乳中最主要的过敏原之一，然而SOCS3对山羊(Capra hircus) αS1-酪蛋白合成的... 详细信息

细胞因子信号转导抑制因子3 (suppressor of cytokine signaling 3, SOCS3)是一种对信号传导作出应答的蛋白，具有调控乳蛋白合成的作用。αS1-酪蛋白是奶畜乳中最主要的过敏原之一，然而SOCS3对山羊(Capra hircus) αS1-酪蛋白合成的影响尚不清楚。为了阐明SOCS3在山羊乳腺中的基因序列、结构、表达情况以及SOCS3对αS1-酪蛋白表达的影响，本研究以安徽白山羊乳腺组织为实验材料，通过PCR克隆SOCS3基因编码区序列，利用在线软件对其进行生物信息学分析，检测SOCS3在不同泌乳时期的表达情况，干扰与过表达SOCS3进行功能分析。结果表明，本研究成功克隆得到山羊SOCS3基因完整编码区为690 bp，编码229个氨基酸；蛋白分子量为25.09 k D，理论等电点为8.97，存在34个磷酸化位点，无信号肽和跨膜螺旋结构，为携带正电荷的不稳定蛋白；SOCS3蛋白与SOCS2、Janus激酶(Janus kinase,JAK)、酪氨酸激酶2 (tyrosine kinase 2, TYK2)、胰岛素受体底物2 (insulin receptor substrate 2, IRS2)、瘦素受体(leptin receptor, LEPR)和白介素6 (interleukin 6, IL6)等蛋白存在互作关系；山羊SOCS3基因与绵羊(Ovis aries)亲缘关系最近，其次是牛(Bos taurus);SOCS3基因在泌乳初期和盛期表达量最高。在山羊乳腺上皮细胞中，干扰SOCS3基因显著上调αS1-酪蛋白的表达水平，过表达SOCS3基因显著下调αS1-酪蛋白的表达水平。以上结果表明，SOCS3在山羊αS1-酪蛋白合成中具有负向调控作用。本研究为开展SOCS3基因在山羊αS1-酪蛋白合成中的调控机制研究提供基础。

关键词：安徽白山羊细胞因子信号转导抑制因子3 (SOCS3) 生物信息学分析 αS1-酪蛋白

miR-99a-5p inhibits target gene FZD5 expression and steroid hormone secretion from goat ovarian granulosa cells

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Journal of Integrative Agriculture 2022年第4期21卷 1137-1145页

作者： ZHU Lu JING Jing QIN Shuai-qi LU Jia-ni ZHU Cui-yun ZHENG Qi LIU Ya FANG Fu-gui LI yun-sheng zhang yun-hai LING Ying-hui College of Animal Science and Technology Anhui Agricultural UniversityHefei 230036P.R.China Local Animal Genetic Resources Conservation and Biobreeding Laboratory of Anhui Province Hefei 230036P.R.China

MicroRNA(miRNA) has vital regulatory effects on the proliferation, differentiation and secretion of ovarian granulosa cells, but the role of miR-99a-5p in goat ovarian granulosa cells(GCs) is unclear. Both miR-99a-5p and Frizzled-5(FZD5) were found to be expressed in GCs in goat ovaries via fluorescence in situ hybridization and immunohistochemistry, respectively, and FZD5 was verified(P<0.001) as a target gene of miR-99a-5p by double luciferase reporter gene experiments. Furthermore, FZD5 mRNA and protein expression were both found to be regulated(P<0.05) by miR-99a-5p in GCs. Moreover, the overexpression of miR-99a-5p or knockdown of FZD5 suppressed(P<0.05) estradiol and progesterone secretion from the GCs, as determined by ELISA. In summary, miR-99a-5p inhibits target gene FZD5 expression and estradiol and progesterone synthesis in GCs. Our study thus provides seminal data and new insights into the regulatory mechanisms of follicular development in the goat and other animals.

关键词： miRNA Frizzled-5 follicular development estradiol progesterone