MicroRNA-370-5p inhibits pigmentation and cell proliferation by downregulating mitogen-activated protein kinase kinase kinase 8 expression in sheep melanocytes

作     者：JI Kai-yuan WEN Ru-jun WANG Zheng-zhou TIAN Qian-qian ZHANG Wei ZHANG Yun-hai JI Kai-yuan;WEN Ru-jun;WANG Zheng-zhou;TIAN Qian-qian;ZHANG Wei;ZHANG Yun-hai

作者机构：Anhui Province Key Laboratory of Veterinary Pathobiology and Disease ControlCollege of Animal Science and TechnologyAnhui Agricultural UniversityHefei 230036P.R.China Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resources Conservation and Bio-BreedingCollege of Animal Science and TechnologyAnhui Agricultural UniversityHefei 230036P.R.China Linquan Comprehensive Experimental StationAnhui Agricultural UniversityLinquan 236400P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2023年第22卷第4期

页      面：1131-1141页

核心收录：

学科分类：0905[农学-畜牧学] 09[农学] 

基　　金：funded by the Natural Science Foundation of Anhui Province,China(2008085QC158) the University Natural Science Research Project of Anhui Province(KJ2019A0165) 

主　　题：microRNA mitogen-activated protein kinase kinase kinase 8 melanogenesis sheep melanocytes cell proliferation 

摘      要：In mammals,microRNAs(miRNAs)play key roles in multiple biological processes by regulating the expression of target *** have found that the levels of miR-370-5p expression differ significantly in the skins of sheep with different hair colors;however,its function remains *** this study,we investigated the roles of miR-370-5p in sheep melanocytes and found that the overexpression of miR-370-5p significantly inhibited cell proliferation(P0.01),tyrosinase activity(P=0.001)and significantly reduced(P0.001)melanin *** prediction revealed that the 3′-untranslated region(UTR)of MAP3K8 has a putative miR-370-5p binding site,and the interaction between these two molecules was confirmed using luciferase reporter *** situ hybridization assays revealed that MAP3K8 is expressed in the cytoplasm of *** results of quantitative RT-PCR and Western blotting analyses revealed that overexpression of miR-370-5p in melanocytes significantly inhibits(P0.01)MAP3K8 expression via direct targeting of its 3′*** of MAP3K8 expression by siRNA-MAP3K8 transfection induced a significant inhibition(P0.01)of melanocyte proliferation and significant reduction(P0.001)in melanin production,which is consistent with our observations for *** gene rescue experiments indicated that the expression of MAP3K8 in melanocytes co-transfected with miR-370-5p and MAP3K8-cDNA(containing sites for the targeted binding to miR-370-5p)was significantly rescued(P≤0.001),which subsequently promoted significant increases in cell proliferation(P0.001)and melanin production(P0.01).Collectively,these findings indicate that miR-370-5p plays a functional role in inhibiting sheep melanocyte proliferation and melanogenesis by downregulating the expression of MAP3K8.