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口蹄疫病毒AF72株3D聚合酶基因的克隆及其在大肠杆菌中的表达

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华北农学报 2008年第6期23卷 32-35页

作者：张昱王永录张永光潘丽中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部畜禽病毒学重点开放实验室甘肃兰州730046

采用RT-PCR方法扩增口蹄疫病毒(FMDV)AF72株的3D聚合酶基因,并将其克隆至pGEM-T easy载体,测序分析结果表明,AF72 3D聚合酶基因与GenBank中公布的其他4个参考序列均具有较高的同源性。将目的基因插入原核表达载体pET-30a(+)中,构建了重... 详细信息

采用RT-PCR方法扩增口蹄疫病毒(FMDV)AF72株的3D聚合酶基因,并将其克隆至pGEM-T easy载体,测序分析结果表明,AF72 3D聚合酶基因与GenBank中公布的其他4个参考序列均具有较高的同源性。将目的基因插入原核表达载体pET-30a(+)中,构建了重组表达质粒pET-3D,鉴定后转化至大肠杆菌BL21(DE3)中,经IPTG诱导,3D聚合酶基因在大肠杆菌中获得了正确表达,目的蛋白的分子量为46 ku。Western blot检测结果显示,表达产物可以与抗A型FMDV阳性血清发生特异性反应,具有良好的反应原性。

关键词：口蹄疫病毒 3D聚合酶基因原核表达

Quantum Computation Using Controllable Exchange Interactions and a Global Magnetic Field

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Quantum Computation Using Controllable Exchange Interactions...

第十二届全国量子光学学术会议

作者： YE Ming-yong, zhang yong-sheng, GUO guang-can (Key Laboratory of Quantum Information, University of Science and Technology of China, Hefei 230026, China)

＜正＞A solid-state quantum computation protocol using controllable Heisenberg exchange interaction and a controllable global magnetic field is proposed. In this protocol practical pulses can be used for controlling e...

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可变形反射镜的研究进展及应用

半导体光电 2006年第6期27卷 659-663,736页

作者：向东王青玲张光勇吕涛张小妮中国地质大学数学与物理学院中国地质大学地球物理与空间信息学院湖北武汉430074 激光国家重点实验室湖北武汉430074

介绍了国内外几种技术比较成熟的可变形反射镜的工作原理,分析了它们的优缺点。针对作者设计、研制的两种可变形反射镜及相关成果进行了评述。利用自主研发的测试系统,对可变形反射镜的特性进行了测试,并且预测了可变形反射镜的发展方向。

关键词：自适应光学可变形反射镜波前校正特性

Loss of membranous carcinoembryonic antigen-related cell adhesion molecule 1 expression is related to decreased relapse-free survival of hepatocellular carcinoma following liver transplantation

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Chinese Medical Journal 2012年第16期125卷 2841-2845页

作者： SHA Qing-quan WEI Qi-zhen ZHU Jian-kan WANG Ke-xin WANG Chao LIU Hai-tao YU Wen-bin LI Ming-xia zhang guang-yong Department of Clinical Medicine Shandong University School of Medicine Jinan Shandong 250012 China Department of General Surgery Qilu Hospital of Shandong University Jinan Shandong 250012 China First People's Hospital of Ningyang County Tai'an Shandong 271400 China School of Chemistry Shandong University Jinan Shandong 250012 China

Background Loss of carcinoembryonic antigen-related cell adhesion molecule 1 （CEACAM1） expression is an adverse prognostic factor in hepatocellular carcinoma （HCC）. The purpose of this study was to investigate the expression of CEACAM1 and its effect on relapse-free survival （RFS） following liver transplantation （LT） for HCC. Methods Expression of CEACAM1 was immunohistochemically detected in HCC specimens from 48 patients. The relationship between CEACAM1 expression and clinicopathologic variables, as well as tumor recurrence, was further analyzed. Results Of the 48 HCC specimens, membranous CEACAM1 expression was detected in 25 specimens and cytoplasmic CEACAM1 expression was detected in 19 specimens. Four specimens had loss of CEACAM1 expression. Loss of membranous CEACAM1 expression was significantly associated with tumor size, tumor number, and serum （z-fetoprotein levels （all P 〈0.05）. Patients with loss of membranous CEACAM1 had significantly poorer RFS than patients with membranous expression, determined via Kaplan-Meier analysis （P=0.027）. Multivariate analysis revealed that loss of membranous CEACAM1 expression might be an independent prognostic factor of RFS for HCC patients after liver transplantation （P=0.037）. Conclusion Loss of membranous CEACAM1 expression in HCC was closely associated with aggressive tumor biology and might be a relapsing biomarker of HCC treated with LT.

关键词： carcinoembryonic antigen-related cell adhesion molecule 1 hepatocellular carcinoma,"liver transplantation," recurrence

对民国时期金陵女子大学“女性取向”的体育教育考察

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妇女研究论丛 2007年第3期 33-38页

作者：张永广李亚娟华中师范大学中国近代史研究所湖北武汉430079 华东师范大学中国语言文学系上海200061

金陵女子大学体育系作为民国时期女子体育教育的典范,积极倡导“女性取向”的办学理念,培养了一批高规格的女子体育人才。金陵女大体育系的理论与实践,不仅促进了女性的身体健康,也有利于国家的振兴。

关键词：体育教育女性金陵女子大学

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口蹄疫诊断技术研究进展

畜牧与兽医 2009年第9期41卷 101-104页

作者：张淑刚张永光中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部畜禽病毒学重点开放实验室农业部草食动物疫病重点开放实验室甘肃兰州730046

口蹄疫是世界上危害最严重的动物疾病之一,可使世界范围内的畜牧业遭受严重的经济损失,并危害人体健康。而防制口蹄疫的重要环节是要作出及时、准确的诊断。本文对口蹄疫新型诊断技术包括基因芯片技术、免疫层析快速诊断试纸条、实时荧... 详细信息

口蹄疫是世界上危害最严重的动物疾病之一,可使世界范围内的畜牧业遭受严重的经济损失,并危害人体健康。而防制口蹄疫的重要环节是要作出及时、准确的诊断。本文对口蹄疫新型诊断技术包括基因芯片技术、免疫层析快速诊断试纸条、实时荧光定量PCR技术、生物传感器的研究进展进行了介绍。

关键词：口蹄疫基因芯片技术免疫层析快速诊断试纸条实时荧光定量PCR技术生物传感器

唯物辩证法本质和核心的两种解读——卢卡奇与列宁之异同

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武汉理工大学学报（社会科学版） 2014年第4期27卷 682-687页

作者：谭桂娟张永光中国人民大学马克思主义学院北京100872 太原科技大学思政部山西太原030024

唯物辩证法又称辩证唯物主义,是马克思主义哲学的重要组成部分。马克思主义哲学产生以来,就存在一个对马克思主义哲学尤其是唯物辩证法本质的理解问题。关于唯物辩证法的本质和核心,卢卡奇认为,总体范畴(总体性)是唯物主义辩证法的本质... 详细信息

唯物辩证法又称辩证唯物主义,是马克思主义哲学的重要组成部分。马克思主义哲学产生以来,就存在一个对马克思主义哲学尤其是唯物辩证法本质的理解问题。关于唯物辩证法的本质和核心,卢卡奇认为,总体范畴(总体性)是唯物主义辩证法的本质,列宁则认为对立统一是辩证法的实质或核心。由于二人的理论传统不同,所处斗争条件不同,批判论敌的侧重点不同,决定了他们对马克思主义辩证法本质的理解存在着差异,他们的结论既有联系又有差别。

关键词：唯物辩证法本质核心卢卡奇列宁

Structural designs and mechanism insights into electrocatalytic oxidation of 5-hydroxymethylfurfural

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Journal of Energy Chemistry 2025年第1期100卷 792-814页

作者： Jing Lei Huijie zhang Jian Yang Jia Ran Jiqiang Ning Haiyan Wang yong Hu Key Laboratory of the Ministry of Education for Advanced Catalysis Materials Department of Chemistry Zhejiang Normal University Jinhua Advanced Research Institute Department of Optical Science and Engineering Fudan University College of Chemistry and Materials Engineering Zhejiang A&F University

Biomass conversion offers an efficient approach to alleviate the energy and environmental *** oxidation of 5-hydroxymethylfurfural(HMF) has attracted tremendous attention in the latest few years for the mild synthesis conditions and high conversion efficiency to obtain 2,5-furan dicarboxylic acid(FDCA), but there still remain problems such as limited yield, short cycle life,and ambiguous reaction mechanism. Despite many reviews highlighting a variety of electrocatalysts for electrochemical oxidation of HMF, a detailed discussion of the structural modulation of catalyst and the underlying catalytic mechanism is still lacking. We herein provide a comprehensive summary of the recent development of electrochemical oxidation of HMF to FDCA, particularly focusing on the mechanism studies as well as the advanced strategies developed to regulate the structure and optimize the performance of the electrocatalysts, including heterointerface construction, defect engineering,single-atom engineering, and in situ reconstruction. Experimental characterization techniques and theoretical calculation methods for mechanism and active site studies are elaborated, and challenges and future directions of electrochemical oxidation of HMF are also prospected. This review will provide guidance for designing advanced catalysts and deepening the understanding of the reaction mechanism beneath electrochemical oxidation of HMF to FDCA.

关键词： Structural design

Imperatorin Is Responsible for the Vasodilatation Activity of Angelica Dahurica var. Formosana Regulated by Nitric Oxide in an Endothelium-Dependent Manner

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Chinese Journal of Integrative Medicine 2009年第6期15卷 442-447页

作者：聂红孟兰贞周玖瑶樊晓峰罗勇张广文 College of Pharmacy Jinan University guangzhou University of Chinese Medicine Research Center for Analytical Sciences of Northeastern University College of Science and Engineering Jinan University

Objective:To demonstrate the vasodilatation activity of the coumarin-containing Angelica dahurica *** and to further analyze active components in the herb ***:(1) The vasodilatation effects induced by different extracts(cyclohexane,ethyl acetate,acetone,methanol,95%ethanol and water) of Angelica dahurica *** on mouse thoracic aorta pre-contracted with phenylephrine were investigated. (2) The amount of imperatorin and isoimperatorin in each extract was measured by high-perfor...

关键词： Angelica dahurica var.formosana thoracic aorta vasodilatation imperatorin isoimperatorin high-performance liquid chromatography