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Blind receiver for OFDM systems via sequential Monte Carlo in factor graphs

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2007年第1期8卷 1-9页

作者： CHEN Rong zhang hai-bin XU You-yun LIU Xin-zhao Department of Electronic Engineering Shanghai Jiao Tong University Shanghai 200240 China Institute of Communication Engineering PLA University of Science and Technology Nanjing 210007 China

Estimation and detection algorithms for orthogonal frequency division multiplexing (OFDM) systems can be de-veloped based on the sum-product algorithms, which operate by message passing in factor graphs. In this paper, we apply the sampling method (Monte Carlo) to factor graphs, and then the integrals in the sum-product algorithm can be approximated by sums, which results in complexity reduction. The blind receiver for OFDM systems can be derived via Sequential Monte Carlo (SMC) in factor graphs, the previous SMC blind receiver can be regarded as the special case of the sum-product algorithms using sampling methods. The previous SMC blind receiver for OFDM systems needs generating samples of the channel vector assuming the channel has an a priori Gaussian distribution. In the newly-built blind receiver, we generate samples of the virtual-pilots instead of the channel vector, with channel vector which can be easily computed based on virtual-pilots. As the size of the vir-tual-pilots space is much smaller than the channel vector space, only small number of samples are necessary, with the blind de-tection being much simpler. Furthermore, only one pilot tone is needed to resolve phase ambiguity and differential encoding is not used anymore. Finally, the results of computer simulations demonstrate that the proposal can perform well while providing sig-nificant complexity reduction.

关键词： Orthogonal frequency division multiplexing (OFDM) Factor graphs Sequential Monte Carlo (SMC) Blind receiver Virtual-pilot

Separation and purification of deoxynivalenol(DON) mycotoxin from wheat culture using a simple two-step silica gel column chromatography

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Journal of Integrative Agriculture 2016年第3期15卷 694-701页

作者： ZHAO Xiu-mei LI Rong-jia ZHOU Chuang zhang Jie HE Cheng-hua ZHENG Ya-ting WU Wen-da zhang hai-bin College of Veterinary Medicine Nanjing Agricultural University

Deoxynivalenol（DON） is a type B trichothecenes mycotoxin produced by several Fusarium species, often found in foodstuffs for humans and animals. DON is in great demand for the toxicological researches both in vivo and in vitro. In this work, wheat culture was inoculated with a Fusarium graminearum PH-1 strain for DON production. The solvent system for crude extraction was acetonitrile-water（84：16, v/v）. A simple two-step silica gel column chromatography was employed to separate the DON mycotoxin from wheat culture, combined with preparative high performance liquid chromatography（preparative HPLC） to purify the compound. The solvent system for the second silica gel column chromatography was methylene chloride-methanol（17：1, v/v）, which provided a good elution effect selected on thin layer chromatography（TLC）. The target compound was identified by HPLC, and the chemical structure was confirmed by mass spectrometry（MS） and ~1H and ^（13）C nuclear magnetic resonance（NMR） spectroscopy. A total of 433 mg of purified DON was obtained from 1 kg of wheat culture, with a purity of 99.01%. The study had provided an easy-operating and cost-effective method to isolate an expensive compound in a simple way.

关键词： deoxynivalenol silica gel column chromatography separation purification

Characterization and Comparison of Ochratoxin A-Ovalbumin(OTA-OVA) Conjugation by Three Methods

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Journal of Integrative Agriculture 2014年第5期13卷 1130-1136页

作者： WANG Xi-chun BAO Ming WU Jin-jie LUO Ying MA Liang-you WANG Ying zhang Ai-hua HE Cheng-hua zhang hai-bin College of Animal Science and Technology Anhui Agricultural University Aquatic Technology Promotion Station of Anhui Province College of Veterinary Medicine Nanjing Agricultural University

In order to generate an antibody against a small hapten molecule, the hapten is cross-linked with carrier protein to make it immunogenic. In this study, the hapten （ochratoxin A, OTA） was coupled to ovalbumin （OVA） by an active ester reaction. To develop a technique for detecting the conjugation, the hapten-protein conjugate （OTA-OVA） was characterized thoroughly by immunoarray technology, ultraviolet （UV） spectroscopy and matrix-assisted laser desorption ionization time-of-flight mass spectrometry （MALDI-TOF-MS）, respectively. The molecular weight of OTA-OVA was 50 350.141 Da, and the molecular weight of OVA was 44 887.506 Da, which were determined by MALDI-TOF-MS, respectively. In OTA-OVA, the molecular coupling ratio was 13：1 by MALDI-TOF-MS while the molecular coupling ratio was 10：1 by UV. In this experiment, UV and MALDI-TOF-MS were selected as the efficient methods to evaluate the cross-linking effect and calculate the molecular coupling ratio.

关键词： ochratoxin A conjugated antigen cartier protein coupling ratio

Antitumor immunopreventive and immunotherapeutic effect in mice induced by hybrid vaccine of dendritic cells and hepatocarcinoma in vivo

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World Journal of Gastroenterology 2003年第3期9卷 479-484页

作者： Jin-Kun zhang Jun Li Juan zhang hai-bin Chen Su-Biao Chen Cancer Pathology Laboratory, Shantou University Medical College, Shantou 515031, Guangdong Province, China Cancer Pathology Laboratory Shantou University Medical College 22 Xinlinglu Shantou 515031 Guangdong Province China. jkzhang@***

AIM: To develop atumor vaccine by fusion of H22 hepatocarcinoma cells and DC, and to study its protective and therapeutical effect against H22 ***: H22-DC vaccine was produced by PEG fusion of H22 and DC induced by cytokine released from splenic mononuclear cells, sorted by CD11c magnetic microbead marker. It was injected through the tail vein of the mice and the H22-DC oncogenesis was detected in the liver, spleen and lung. In order to study the therapeutical and protective effect of H22-DC against tumor H22, two groups were divided:immune group and therapeutic group. Immune group was further divided into P, D, HD and H subgroups, immunized by PBS, DC, H22-DC and inactivated H22, respectively, and attacked by H22 cell. The tumor size, tumor weight, mice survival time and tumor latent period were recorded and statistically analyzed; Therapeutical group was divided into three subgroups of P, D and HD, and attacked by H22, then treated with PBS, DC, and H22-DC, respectively. Pathology and flow cytometry were also applied to study the mechanism how the H22-DC vaccine attacked on the H22 ***: 1. No oncogenesis was found in spleen, lung and liver after H22-DC injection. 2. Hybrid vaccine immunized mice had strongest CTL activity. 3. In the immune group,latent period was longer in HD subgroup than that in P, H and D subgroup; and tumor size and weight were smaller in HD subgroup than that in P, H and D subgroup. 4. In therapeutic group, tumor size was smaller in HD subgroup than that in P, D ***: 1. H22-DC tumor vaccine is safe without oncogenesis in vivo. 2. Hybrid vaccine can stimulate potent specific CTL activity against H22.3. H22-DC vaccine has distinctive prophylatic effect on tumor H22 and can inhibit the tumor growth.

关键词：树状细胞杂合疫苗免疫抑制免疫疗法肝细胞癌动物实验

The impacts of U.S. withdrawal from the Paris Agreement on the carbon emission space and mitigation cost of China, EU, and Japan under the constraints of the global carbon emission space

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Advances in Climate Change Research 2017年第4期8卷 226-234页

作者： DAI Han-Cheng zhang hai-bin WANG Wen-Tao College of Environmental Sciences and Engineering Peking University Beijing 10087 China School of International Studies Peking University Beijing 10087 China The Administrative Center for China's Agenda 21 Ministry of Science and Technology Beijing 100038 China

Based on the Computable General Equilibrium (CGE) model and scenario analysis, the impacts of the U.S. withdrawal from the Paris Agreement on the carbon emission space and mitigation cost in China, European Union (EU), and Japan are assessed under Nationally Determined Contributions (NDCs) and 2 C scenarios due to the changed emission pathway of the U.S. The results show that, under the condition of constant global cumulative carbon emissions and a fixed burden-sharing scheme among countries, the failure of the U.S. to honor its NDC commitment to different degrees will increase the U.S. carbon emission space and decrease its mitigation cost. However, the carbon emission space of other parties, including China, EU, and Japan, will be reduced and their mitigation costs will be increased. In 2030, under the 2 C target, the carbon price will increase by 4.4e14.6 US$ t1 in China, by 9.7e35.4 US$ t1 in the EU, and by 16.0e53.5 US$ t1 in Japan. In addition, China, EU, and Japan will incur additional Gross Domestic Production (GDP) loss. Under the 2 C target, the GDP loss of China would increase by US$22.0e71.1 billion (equivalent to 16.4e53.1 US$ per capita), the EU's GDP loss would increase by US$9.4e32.1 billion (equivalent to 20.7e71.1 US$ per capita), and Japan's GDP loss will increase by US$4.1e13.5 billion (equivalent to 34.3e111.6 US$ per capita).

关键词： U.S. withdrawal from Paris Agreement Carbon emission space Mitigation cost

U.S. withdrawal from the Paris Agreement: Reasons, impacts, and China's response

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Advances in Climate Change Research 2017年第4期8卷 220-225页

作者： zhang hai-bin DAI Han-Cheng Hua-Xia LAI WANG Wen-Tao School of International Studies Peking University Beijing 100871 China College of Environmental Sciences and Engineering Peking University Beijing 100871 China School of Law University of Washington Seattle 98195-5852 USA The Administrative Center for China's Agenda 21 Ministry of Science and Technology Beijing 100038 China

Applying qualitative and quantitative methods, this article explains the driving forces behind U.S. President Donald Trump's decision to withdraw from the Paris Agreement, assesses the impacts of this withdrawal on the compliance prospects of the agreement, and proposes how China should respond. The withdrawal undercuts the foundation of global climate governance and upsets the process of climate cooperation, and the impacts are manifold. The withdrawal undermines the universality of the Paris Agreement and impairs states' confidence in climate cooperation; it aggravates the leadership deficit in addressing global climate issues and sets a bad precedent for international climate cooperation. The withdrawal reduces other countries' emission space and raises their emission costs, and refusal to contribute to climate aid makes it more difficult for developing countries to mitigate and adapt to climate change. Cutting climate research funding will compromise the quality of future IPCC reports and ultimately undermine the scientific authority of future climate negotiations. China faces mounting pressure from the international community to assume global climate leadership after the U.S. withdraws, and this article proposes that China should reach the high ends of its domestic climate targets under the current Nationally Determined Contributions; internationally, China should facilitate the rebuilding of shared climate leadership, replacing the G2 with C5. Meanwhile, China needs to keep the U.S. engaged in climate cooperation.

关键词： U.S. withdrawal from the Paris Agreement Compliance Global climate governance China

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临界装置数字化人机界面的分析与实现

原子能科学技术 2012年第B9期46卷 426-431页

作者：潘建张海斌中国工程物理研究院核物理与化学研究所四川绵阳621900

为了建立信息化程度高、扩展灵活、适合操纵人员需要的全新数字化系统,将数字化人机界面(HMI)技术应用于临界装置控制系统中。通过对临界装置数字化人机界面的分析,与人因工程原则相适应,描述了符合临界装置运行习惯和任务处理特点的数... 详细信息

为了建立信息化程度高、扩展灵活、适合操纵人员需要的全新数字化系统,将数字化人机界面(HMI)技术应用于临界装置控制系统中。通过对临界装置数字化人机界面的分析,与人因工程原则相适应,描述了符合临界装置运行习惯和任务处理特点的数字化运行理念,最终实现了临界装置的数字化控制监测保护系统,并投入稳定运行。

关键词：人机界面控制监测保护系统人因工程数字化运行理念

Effect of a Microbiological Detoxification Agent on Mycotoxin in Feed Pigs

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Agricultural Sciences in China 2002年第11期1卷 1266-1270页

作者： zhang hai-bin and LU Cheng-ping(Nanjing Agricultural University , Nanjing 210095 ,P. R. China) Nanjing Agricultural University Nanjing 210095 P.R.China

Biological method is an ideal way to remove the toxin from contaminated feed compared with both physical and chemical methods. A new microbiological agent, BH was used to reduce the toxicity of mildewed wheat containing deoxynivalenol(DON) at 22. 7 mg per kg feed. Experimental animals included two batches of 24 pigs (5 kg ) and 48 pigs (30 kg). Each batch was randomly divided into 4 groups fed with diets supplemented with different agents as follows: Group 1, +DON; Group2, +DON + BH; Group3, +BH; Group4, just the routine diet as control. The pigs were weighed every 2 weeks, while blood biochemical items were detected every 3 weeks during the experimental period of 6 weeks. The results showed that DON significantly reduced the weight gain of both batches. BH greatly alleviated the harm of DON to the animals. However, BH alone had no influence on weight gain. Furthermore, DON increased some biochemical items significantly, such as AST and GPT. In vitro, BH reduced the amount of DON in liquid medium, while BH added mildew feed did not apparently affect the content of DON in feed if they did not pass the gastrointestine.

关键词： Pig Deoxynivalenol BH

Antitumor activities of human dendritic cells derived from peripheral and cord blood

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World Journal of Gastroenterology 2002年第1期8卷 87-90页

作者： Jin-Kun zhang Jun Li hai-bin Chen Jin-Lun Sun Yao-Juan Qu Juan-Juan Lu Cancer Pathology Laboratory,Shantou University,Medical College,Shantou 515031,Guangdong Province,China Cancer Pathology Laboratory Shantou University Medical College 22 Xinlinglu Shantou 515031 Guangdong Province China. jkzhang@***

AIM: To observe the biological specialization of humanperipheral blood dendritic cells (DC) and cord blood derivedDC and its effects on effector cells killing humanhepatocarcinoma cell line BEL-7402 in ***: The DC biological characteristics were detectedwith immunohistochemical and MTT assay. Two antitumorexperimental groups are: peripheral blood DC and cordblood DC groups. Peripheral blood DC groups used LAKcells as the effector cells and BEL-7402 as target cells, whilecord blood DC groups used CTL induced by tumor antigentwice pulsed DC as effector cells and BEL-7402 as targetcells, additional peripheral blood DC and cord blood DC areadded to observe its stimulating activities to effector *** effector's cytotoxicity to tumor cells were detected withneutral red colorimetric assay at two effector/target ratios of5:1 and 10: ***: Peripheral blood DC and cord blood DC highlyexpressed HLA-ABC, HLA-DR, HLA-DQ, CD54 and S-100protein. The stimulating activities to lymphocyteproliferation were compared between experimental groups(DC added) and control group (no DC added). In sixexperiment subgroups, the DC/lymphocyte ratio wassequentially 0.25: 100, 0.5: 100, 1: 100, 2: 100, 4: 100 and 8:100, A values(x± s) were 0.75396± 0.009, 0.84916± 0.010,0.90894± 0.012, 0.98371 ± 0.007, 1.01299 ± 0.006 and 1.20384± 0.006 in peripheral blood DC groups and 0.77650 ± 0.005,0.83008± 0.007, 0.92725 ± 0.007, 1.05990 ± 0.010, 1.15583 ±0.011, 1.22983 ± 0.011 in cord blood DC groups. A value was0.59517 ± 0.005 in control group. The stimulating activitieswere higher in experimental groups than in control group ( P＜ 0.01 ), which were increased when the DC concentrationwas enlarged ( P ＜ 0.01 ). Two differently derived DCs hadthe same phenotypes and similar stimulating activities ( P ＞0.05). In peripheral blood DC groups, the cytotoxicity (x ±s) of the LD groups (experimental groups) and L groups(control group) was 58.16% ± 2.03% (5: 1), 46.18% ±2.25% (10:l) and 38.13% ± 1.

关键词：肝癌树突状细胞抗肿瘤机制外周血脐带血