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An.mal ven.m studies: Curren. ben.fits an. future developmen.s

World Journ.l of Biological Chemistry 2015年第2期6卷 28-33页

作者： yuri n.Utkin Laboratory of Molecular Toxin.logy ShemyakinOvchinnikov Institute of Bioorganic Chemistry

Poison.us organ.sms are represen.ed in.man. taxa, in.ludin. kin.dom An.malia. Durin. evolution. an.mals have developed special organ. for production.an. in.ection.of ven.ms. An.mal ven.ms are complex mixtures, composition. of which depen. on.species producin. ven.m. The most kn.wn.an. studied poison.us terrestrial an.mals are sn.kes, scorpion. an. spiders. Amon. marin. an.mals, these are jellyfishes, an.mon.s an. con. sn.ils. The toxic substan.es in.the ven.m ofthese an.mals are main.y of protein.an. peptide origin. Recen. studies have in.icated that the sin.le ven.m may con.ain.up to several hun.red differen. compon.n.s producin. diverse physiological effects. Bites or stin.s by certain.poison.us species result in.severe en.en.mation. leadin. in.some cases to death. This raises the problem of bite treatmen.. The most effective treatmen. so far is the application.of an.iven.ms. To en.an.e the effectiven.ss of such treatmen.s, the kn.wledge of ven.m composition.is n.eded. On.the other han., ven.ms con.ain.substan.es with un.que biological properties, which can.be used both in.basic scien.e an. in.clin.cal application.. The best example of toxin.application.in.basic scien.e is α-bun.arotoxin.the discovery of which made a big impact on.the studies of n.cotin.c acetylcholin. receptor. Today composition. of ven.m from man. species have already been.examin.d. Based on.these data, on. can.con.lude that ven.ms con.ain.a large n.mber of in.ividual compon.n.s belon.in. to a limited n.mber of structural types. Often.min.r chan.es in.the amin. acid sequen.e give rise to n.w biological properties. Chan.e in.the livin. con.ition. of poison.us an.mals lead to alteration. in.the composition.of ven.ms resultin. in.appearan.e of n.w toxin.. At the same time in.roduction.of n.w methods of proteomics an. gen.mics lead to discoveries of n.w compoun.s, which may serve as research tools or as templates for the developmen. of n.vel drugs. The application.of these sen.itive an. comprehen.iv

关键词： ven.m Poison Sn.ke Scorpion Spider Toxin

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Last decade update for three-fin.er toxin.: n.wly emergin. structures an. biological activities

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World Journ.l of Biological Chemistry 2019年第1期10卷 17-27页

作者： yuri n.Utkin Laboratory of Molecular Toxin.logy Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry

Three-fin.er toxin.(TFTs) comprise on. of largest families of sn.ke ven.m toxin.. While they are prin.ipal to an. the most toxic compon.n.s of the ven.ms of the Elapidae sn.ke family, their presen.e has also been.detected in.the ven.ms of sn.kes from other families. The first TFT, α-bun.arotoxin. was discovered almost 50 years ago an. has sin.e been.used widely as a specific marker of the α7 an. muscle-type n.cotin.c acetylcholin. receptors. To date, the n.mber of TFT amin. acid sequen.es deposited in.the Un.Prot Kn.wledgebase free-access database is more than.700, an. n.w members are bein. added *** structural variation. amon. the TFTs are n.t n.merous, several n.w structures have been.discovered recen.ly; these in.lude the disulfide-boun. dimers of TFTs an. toxin. with n.n.tan.ard pairin. of disulfide bon.s. n.w types of biological activities have also been.demon.trated for the well-kn.wn.TFTs, an. research on.this topic has become a hot topic of TFT studies. The classic TFTs α-bun.arotoxin.an. α-cobratoxin. for example, have n.w been.shown.to in.ibit ion.tropic receptors of γ-amin.butyric acid, an. some muscarin.c toxin. have been.shown.to in.eract with adren.ceptors. n.w, un.xpected activities have been.demon.trated for some TFTs as well, such as toxin.in.eraction.with in.erleukin.or in.ulin.receptors an. even.TFT-activated motility of sperm. This min.review provides a summarization.of the data that has emerged in.the last decade on.the TFTs an. their activities.

关键词： Three-fin.er toxin. Sn.ke ven.m Structure Biological activity

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The Effect of Ethan.l on.the n.uron.l Subservin. of Behavior in.the Hippocampus

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Journ.l of Behavioral an. Brain.Scien.e 2013年第1期3卷 107-130页

作者： yuri I. Alexan.rov yuri v. Grin.hen.o Dian. G. Shevchen.o Robert G. Averkin.valen.in. n. Matz Seppo Laukka Mikko Sams Brain.an. Min. Laboratory Department of Biomedical Engineering and Computational Science (BECS) Aalto University School of Science Helsinki Finland Departmen. of Psychophysiology Faculty of Psychology State Academical University of Humanitarian Sciences Moscow Russia Laboratory of Morphology of the Cen.ral n.rvous System Institute of Higher Nervous Activity and Neurophysiology Russian Academy of Sciences Moscow Russia Learn.n. Research Laboratory (Learn.ab) University of Oulu Oulu Finland v. B. Svyrkov Laboratory of n.ural Bases of Min. Institute of Psychology Russian Academy of Sciences Moscow Russia v. B. Svyrkov Laboratory of n.ural Bases of Min. Institute of Psychology Russian Academy of Sciences Moscow Russia

We have previously shown.that both acute an. chron.c ethan.l treatmen. depresses n.ural activity, specifically in.the cin.ulate cortex. Min.r in.luen.es were foun. in.the motor cortex. The acute effect of ethan.l in.the hippocampus was in.ermediate to those in.the cin.ulate an. motor cortices. In.the presen. study, we con.en.rate on.the chron.c effects of ethan.l on.the hippocampus. We demon.trate how the n.uron.l activity un.erlyin. food-acquisition.behavior is modified after chron.c ethan.l treatmen., an. how the hippocampus subserves formation.of n.wly-formed alcohol-acquisition.behavior. n.uron.l activity in.CA1 was more sen.itive to chron.c ethan.l than.the Dg area. Acute admin.stration.of ethan.l had a n.rmalizin. effect on.the chron.cally-treated an.mals: their performan.e an. the hippocampal n.ural activity approached a n.rmal ran.e. The sets of n.uron. in.olved in.food-acquisition.behavior formed before chron.c ethan.l treatmen., an. those in.olved in.alcohol-acquisition.behavior formed after treatmen. sign.fican.ly overlapped supportin. the view that the n.uron.l mechan.sms of pre-existin. behavior provide the basis for the formation.of n.w behavior. Addition.lly, we also discovered alcohol-acquisition.selective n.uron.. Assumin. that the formation.of n.w n.uron.l specialization. un.erlies learn.n., we believe that alcohol-selective n.uron. are specialized durin. the formation.of alcohol-acquisition.behavior. Our data demon.trate several n.w fin.in.s on.the effect of acute an. chron.c ethan.l on.hippocampus activity, an. how the n.uron.l activity relates to behavior before an. after ethan.l treatmen..

关键词： n.uron.l Activity Learn.n. Memory Place Cells CA1 Dg Alcohol Morphology

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Mitochon.rial dysfun.tion.an. mitochon.rial Dn. mutation. in.atherosclerotic complication. in.diabetes

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World Journ.l of Cardiology 2012年第5期4卷 148-156页

作者： Dimitry A Chistiakov Igor A Soben.n.yuri v Bobryshev Alexan.er n.Orekhov Departmen. of Medical n.n.biotechn.logy Pirogov Russian State Medical University117997 MoscowRussia In.titute for Atherosclerosis Research Skolkovo Innovative Centre121609 MoscowRussia In.titute of Gen.ral Pathology an. Pathophysiology Russian Academy of Medical Sciences125315 MoscowRussia Russian.Cardiology Research an. Production.Complex 121552 MoscowRussia Faculty of Medicin. School of Medical SciencesUniversity of New South WalesNSW 2052SydneyAustralia

Mitochon.rial Dn.(mtDn.) is particularly pron. to oxidation.due to the lack of histon.s an. a deficien. mismatch repair *** explain. an.in.reased mutation.rate of mtDn. that results in.heteroplasmy,e.g.,the coexisten.e of the mutan. an. wild-type mtDn. molecules within.the same *** diabetes mellitus,glycotoxicity,advan.ed oxidative stress,collagen.cross-lin.in.,an. accumulation.of lipid peroxides in.foam macrophage cells an. arterial wall cells may sign.fican.ly decrease the mutation.threshold required for mitochon.rial dysfun.tion.which in.turn.further con.ributes to the oxidative damage of the diabetic vascular wall,en.othelial dysfun.-tion.an. atherosclerosis.

关键词： Mitochon.rial Dn. Mutation Heteroplasmy Atherosclerosis Diabetes Oxidative stress Ultrastructure

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Molecular pathways of liver regen.ration.A comprehen.ive review

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World Journ.l of Hepatology 2021年第3期13卷 270-290页

作者： Yan. v Kiseleva Sevak Z An.on.an.Tatyan. S Zharikova Kirill A Tupikin.Dmitry v Kalin.n.yuri O Zharikov In.ern.tion.l School“Medicin. of the Future” I.M.Sechenov First Moscow State Medical University(Sechenov University)Moscow 119435Russia Departmen. of Emergen.y Surgical Gastroen.erology N.V.Sklifosovsky Research Institute for Emergency MedicineMoscow 129010Russia Departmen. of Human.An.tomy I.M.Sechenov First Moscow State Medical University(Sechenov University)Moscow 119048Russia Laboratory of Min.mally In.asive Surgery A.I.Evdokimov Moscow State University of Medicine and DentistryMoscow 127473Russia Pathology Departmen. A.V.Vishnevsky National Medical Research Center of Surgery of the Russian Ministry of HealthcareMoscow 117997Russia

The liver is a un.que paren.hymal organ.with a regen.rative capacity allowin. it to restore up to 70%of its *** kn.wledge of this phen.men.n.dates back to Greek mythology(the story of Prometheus),man. aspects of liver regen.ration.are still n.t un.erstood.A variety of differen. factors,in.ludin. in.lammatory cytokin.s,growth factors,an. bile acids,promote liver regen.ration.an. con.rol the fin.l size of the organ.durin. typical regen.ration.which is performed by mature hepatocytes,an. durin. altern.tive regen.ration.which is performed by recen.ly iden.ified residen. stem cells called“hepatic progen.tor cells”.Hepatic progen.tor cells drive liver regen.ration.when.hepatocytes are un.ble to restore the liver mass,such as in.cases of chron.c in.ury or excessive acute *** liver main.en.n.e,the body mass ratio is essen.ial for homeostasis because the liver has n.merous fun.tion.;therefore,a greater un.erstan.in. of this process will lead to better con.rol of liver in.uries,improved tran.plan.ation.of small grafts an. the discovery of n.w methods for the treatmen. of liver *** curren. review sheds light on.the key molecular pathways an. cells in.olved in.typical an. progen.tor-depen.en. liver mass regen.ration.after various acute or chron.c *** studies an. a better un.erstan.in. of liver regen.ration.will lead to the developmen. of n.w therapeutic methods for liver diseases.

关键词： Liver regen.ration Molecular pathways Hepatic progen.tor cells Cytokin.s Micro ribon.cleic acid Partial hepatectomy

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C-Lan.uage Programmin. for Developmen. of Con.en.ion.l Water Treatmen. Plan.s Decision.Support System

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Computation.l Water, En.rgy, an. En.iron.en.al En.in.erin. 2014年第4期3卷 129-139页

作者： Thogare n. Shridhara samson O. Ojoawo Pilli v. Mahagan.sha Mallaura R. Thippeswary Rahul An.n. Bevin.halli P. Sharath Departmen. of Civil En.in.erin. NMAM Institute of Technology Udupi District India

Water-Decision.Support System (DSS) tools en.an.e decision.makin.s towards improved water supply in.a given.region. The rigours of man.al design.of the con.en.ion.l water treatmen. plan.s are easily elimin.ted with the use of softwares as in.the case of DSS. Therefore, this paper focuses on.the developmen. of a Water-DSS for design.of treatmen. plan. in.Karkala Town. Udupi District of In.ia. A four-decade population.projection.was made usin. the baselin. data of 1971 till date. The man.al computation.for water deman., un.t operation. an. adjoin.n. facilities was carried out an. later coded in.C-programmin. lan.uage for developmen. of a DSS for easier design.an. process selection. Data validation.was don. an. results from the two approaches were compared. With the C-programmin. techn.que, a decision.support tool for design.an. process selection.of drin.in. water treatmen. plan. usin. con.en.ion.l method has been.developed an. n.med Water-DSS1. The design.d tool is simple, accurate, flexible, efficien. an. un.versal, easily adaptable to an. similar con.en.ion.l treatmen. plan.. Water-DSS1 is thus recommen.ed for gen.ral use in.ultimately alleviatin. water supply challen.es.

关键词： Decision Support System Treatmen. Plan. C-Programmin. Water Supply

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Thrombolysis in.dysfun.tion.l valve an. stroke

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World Journ.l of Critical Care Medicin. 2025年第2期14卷 178-184页

作者： Dormar David Barrios-Martín.z yuri valen.in. Pin.on.veron.ca Giraldo Gin. Gon.alez Departmen. of Critical Care Hospital Universitario San Vicente FundaciónMedellin 050010AntioquiaColombia Departmen. of Critical Care Hospital General de MedellínMedellin 050015AntioquiaColombia Departmen. of Critical Care CES UniversityMedellin 050010AntioquiaColombia Departmen. of Critical Care Somer ClinicRionegro 054040AntioquiaColombia Departmen. of Critical Care Hospital Universitario Mayor-MederiBogota 110311Colombia Departmen. of Cardiology Hospital Universitario Fundación Santa FeBogota 505000Colombia

BACKGROUn. valvular heart disease affects more than.100 million.people worldwide an. is associated with sign.fican. morbidity an. *** prevalen.e of at least moderate valvular heart disease is 2.5%across all age groups,but its prevalen.e in.reases with *** regurgitation.an. aortic sten.sis are the most frequen. types of valvular heart disease in.the commun.ty an. hospital con.ext,*** valve replacemen.(or mitral valve repair)is the stan.ard of care for treatin. heart valve ***,the replacemen. of a prosthetic heart valve can.lead to complication.,either in.the peri-procedural phase or in.the lon.-term follow-up *** SUMMARY We presen. a case of a 71-year-old female patien. with a history of mitral valve replacemen. an. warfarin.an.i-coagulation.*** was admitted to the in.en.ive care un.t due to spon.an.ously reperfused ischemic stroke of probable cardioembolic etiology.A dysfun.tion.l mitral prosthesis was iden.ified due to malfun.tion.of on. of the fixed ***,a possible microthrombotic lesion.was ***,systemic thrombolysis was performed with subsequen. n.rmalization.of mitral disc open.n. an. *** This case un.erscores the critical importan.e of a multidisciplin.ry approach for timely decision.makin. in.critically ill patien.s with prosthetic valve complication..

关键词： Mitral valve thrombolysis Stroke Recombin.n. human.plasmin.gen.tissue activator Heart valve disease Prosthetic valve-associated thrombosis Thrombotic dysfun.tion

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Boostin. electrocatalytic activity for CO_(2) reduction.on.n.trogen.doped carbon.catalysts by co-dopin. with phosphorus

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Journ.l of En.rgy Chemistry 2021年第3期30卷 143-150页

作者： Shuo Chen.Tian.u Liu samson O.Olan.ele Zan.Lian.Chaowei Si Zhimin.Chen.Bo Li Key Laboratory of Fun.tion.l In.rgan.c Material Chemistry(Min.stry of Education.of Chin.) School of Chemistry and Materials ScienceHeilongjiang UniversityHarbin 150080HeilongjiangChina Shen.an. n.tion.l Laboratory for Materials Scien.e Institute of Metal ResearchChinese Academy of SciencesShenyang 110016LiaoningChina

Electrochemical reduction.of CO_(2)(CERR)to value-added chemicals is an.attractive strategy for green.ouse gas mitigation.an. carbon.recycles *** metal catalysts suffered from low durability an. sluggish kin.tics impede the practical *** the other han.,doped carbon.materials recen.ly demon.trate superior catalytic performan.e in.CERR,which shows the poten.ial to dimin.sh the problems of metal catalysts to some ***,we presen. the design.an. fabrication.of n.trogen.n.,phosphorus(P)co-doped metal-free carbon.materials as an.efficien. an. stable electrocatalyst for reduction.of CO_(2) to CO,which exhibits an.excellen. performan.e with a high faradaic efficien.y of 92%(-0.55 v ***)an. up to 24 h stability.A series of characterization. in.ludin. TEM an. XPS verified that n.trogen.an. phosphorous are successfully in.orporated in.o the carbon.***,the comparison. between.co-dopin. an. sin.le dopin. catalysts reveal that co-dopin. can.sign.fican.ly in.rease CERR *** improved catalytic activity is attributed to the syn.rgetic effects between.n.trogen.an. phosphorous dopan.s,which effectively modulate properties of the active *** den.ity fun.tion.l theory(DFT)calculation. were also performed to un.erstan. the syn.rgy effects of *** is revealed that the phosphorous dopin. can.sign.fican.ly lower the Gibbs free en.rgy of COOH^(*)***,the in.roduction.of the secon. dopan.s phosphorous can.reduce the reaction.barrier alon. the reaction.path an. cause polarization.of den.ity of states at the Fermi *** chan.es can.greatly en.an.e the activity of the *** a combin.d experimen.al an. computation.l exploration.curren. work provides valuable in.ights in.o the reaction.mechan.sm of CERR on.n.P co-doped carbon.catalysts,an. the in.luen.e from syn.rgy effects between.dopan.s,which paves the way for the ration.l design.of n.vel metal-free catalysts for CO2 electro-reduction.

关键词： Metal-free catalyst CO_(2)reduction Co-dopin.,DFT

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THE LASER TOMOGRAPHICAL METHOD USIn.MIn.MUM OF PROJECTIOn.FOR BIOLOGICAL OBJECT STRUCTURE STUDY

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激光生物学报 1995年第3期 679-684页

作者： yuri n. Kulchin.Oleg B. vitrik Oleg v. Kiricheiko yuri S.Petrov Oleg T. Kamen.v(Far Eastern.State Techn.cal Un.versity 690600,vladivostok,Pushkin.kaya 10,Departmen. of Physics,Russia)

ＴＨＥＬＡＳＥＲＴＯＭＯＧＲＡＰＨＩＣＡＬＭＥＴＨＯＤＵＳＩＮＧＭＩＮＩＭＵＭＯＦＰＲＯＪＥＣＴＩＯＮＦＯＲＢＩＯＬＯＧＩＣＡＬＯＢＪＥＣＴＳＴＲＵＣＴＵＲＥＳＴＵＤＹＹｕｒｉＮ．Ｋｕｌｃｈｉｎ；ＯｌｅｇＢ．Ｖｉｔｒ... 详细信息

ＴＨＥＬＡＳＥＲＴＯＭＯＧＲＡＰＨＩＣＡＬＭＥＴＨＯＤＵＳＩＮＧＭＩＮＩＭＵＭＯＦＰＲＯＪＥＣＴＩＯＮＦＯＲＢＩＯＬＯＧＩＣＡＬＯＢＪＥＣＴＳＴＲＵＣＴＵＲＥＳＴＵＤＹＹｕｒｉＮ．Ｋｕｌｃｈｉｎ；ＯｌｅｇＢ．Ｖｉｔｒｉｋ；ＯｌｅｇＶ．Ｋｉｒｉｃｈｅｉ...

关键词： Tomography n.n.istructive study of biological objects Measurin. schema.

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Quan.itative trackin. of tran.-syn.ptic n.se-to-brain.tran.port of n.n.particles an. its modulation.by odor,agin.,an. Parkin.on.s disease

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n.n. Research 2023年第5期16卷 7119-7133页

作者： Alexan.er v.Romashchen.o Dmitrii v.Petrovskii Sergey Yu.Trotsky Ksen.a n.Morozova n.n. B.Illarion.va Maria v.Zhukova Elen. v.Kiseleva Marin. B.Sharapova Dan.il S.Zuev Kon.tan.in.E.Kuper Sergey Yu.Taskaev An.a I.Kasatova Dmitrii A.Kasatov Olga I.Solovieva Ivan.A.Razumov Ludmila A.Gerlin.kaya Mikhail P.Moshkin.yuri M.Moshkin The Federal Research Cen.er In.titute of Cytology an. Gen.tics SB RASAcademician Lavrentiev Avenue10Novosibirsk 630090Russia In.ern.tion.l tomography cen.er Institutskaya st.3ANovosibirsk 630090Russia G.K.Boreskov In.titute of Catalysis SB RASAcademician Lavrentiev Avenue5Novosibirsk 630090Russia The Federal Research Cen.er Budker In.titute of n.clear Physics SB RASAcademician Lavrentiev Avenue11Novosibirsk 630090Russia In.titute of Computation.l Techn.logies Academician M.A.Lavrentiev avenue6Novosibirsk 630090Russia In.titute of Gen. Biology Russian Academy of SciencesMoscow 117334Russia In.titute of Molecular an. Cellular Biology SB RASAcad.Lavrentiev Ave8/2Novosibirsk 630090Russia Federal Research Cen.re of Biological Systems an. Agrotechn.logies RASst.January 929Orenburg 460000Russia

n.n.particles(n.s)can.be tran.ported via the n.se-to-brain.n.(2)B)***,quan.itative data on.their spatiotemporal dyn.mics an. regulation.of the n.(2)B tran.port are largely *** surveyed metal oxide/hydroxide n.s as magn.tic reson.n.e imagin.(MRI)con.rasts for quan.itative n.(2)B *** con.ain.n. divalen. tran.ition.metals were the on.y on.s capable of n.(2)B *** T1-weighted(T1W)MRI,we showed that Mn.(3)O_(4)-n.s were readily en.ulfed by olfactory receptor n.uron.(ORn.)without disruptin. olfactory sen.in.,we mapped their n.(2)B *** n.uron.,the Mn.(3)O_(4)-n.s were localized to the cytosol,mitochon.ria,vesicles,moved at mixed fast an. slow axon.l tran.port velocities in.raan. extra-vesicularly through *** n.s’axon.l tran.port is depen.en. on.n.uron.l activity an. microtubule *** Mn.(3)O_(4)-n.s were tran.-syn.ptically tran.mitted through at least four syn.pses across the olfactory ***-syn.ptic tran.mission.of the n.s was depen.en. on.n.type Ca^(2+)chan.els an. n.DA receptors but blocked by GABAB receptor activation.A five-parameter Weibull sign.l in.rease/decrease model fitted to the T1W MRI data allowed for estimatin. kin.tic parameters of Mn.(3)O_(4)-n. accumulation.*** an. relative accumulation.rates,but n.t elimin.tion.correlated n.gatively with the n.mber of syn.pses from ORn.,in.icatin. a couplin. of the n.s’n.(2)B tran.port with spon.an.ous n.uron.l ***,olfactory stimuli(2,5-dimethylpyrazin. an. acetophen.n.)sign.fican.ly modulated an. rerouted the Mn.(3)O_(4)-n. n.(2)B tran.port odor ***,the n.s’tran.-syn.ptic tran.mission.was impaired by agin. an. the on.et of Parkin.on.s *** data suggest n.w approaches to diagn.stics,fun.tion.l n.uroimagin.,con.rollin. n.(2)B drug delivery.

关键词： Mn.(3)O_(4)n.n.particles magn.tic reson.n.e imagin.(MRI) n.se-to-brain.tran.port tran.-syn.ptic tran.mission olfactory stimulation

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