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Population structuring of the ubiquitous stingless bee Tetragonisca angustula in southern Brazil as revealed by microsatellite and mitochondrial markers

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Insect Science 2017年第5期24卷 877-890页

作者： Flavio O. Francisco Leandro R. Santiago yuri m. mizusawa Benjamin P. Oldroyd maria C. Arias Departamento de Genetica e Biologia Evolutiva Instituto de Biociencias Universidade de Sao Paulo Rua do MatZo 277 - sala 320Sao Paulo SP Brazi Behaviour and Genetics of Social Insects Lab School of Life and Environmental Sciences A 12 University of Sydney Sydney NSW Australia

Tetragonisca angustula is one of the most widespread stingless bees in the Neotropics. This species swarms frequently and is extremely successful in urban envi- ronments. In addition, it is one of the most popular stingless bee species for beekeeping in Latin America, so nest transportation and trading is common. Nest transportation can change the genetic structure of the host population, reducing inbreeding and increasing homogenization. Here, we evaluate the genetic structure of 17 geographic populations of T. angustula in southern Brazil to quantify the level of genetic differentiation between populations. Analyses were conducted on partially sequenced mitochondrial genes and 11 microsatellite loci of 1002 workers from 457 sites distributed on the mainland and on 3 islands. Our results show that T. angustula populations are highly differentiated as demon- strated by mitochondrial DNA （mtDNA） and microsatellite markers. Of 73 haplotypes, 67 were population-specific. mtDNA diversity was low in 9 populations but microsatellite diversity was moderate to high in all populations. microsatellite data suggest 10 genetic clusters and low level of gene flow throughout the studied area. However, physical barri- ers, such as rivers and mountain ranges, or the presence or absence of forest appear to be unrelated to population clusters. Factors such as low dispersal, different ecological con- ditions, and isolation by distance are most likely shaping the population structure of this species. Thus far, nest transportation has not influenced the general population structure in the studied area. However, due to the genetic structure we found, we recommend that nest transportation should only occur within and between populations that are genetically similar.

关键词： genetic structure islands meliponini microsatellites mtDNA nest transportation

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Long-term Speckle Interferometric monitoring of Binary Systems:2007–2023Positional measurements and Orbits of Seven Objects

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Research in Astronomy and Astrophysics 2024年第9期24卷 147-155页

作者： Arina mitrofanova Vladimir Dyachenko Anatoly Beskakotov yuri Balega Alexander maksimov Denis Rastegaev Special Astrophysical Observatory 369167 Nizhnij ArkhyzRussia

The results of seventeen years of speckle interferometric monitoring of seven objects(Chara 122Aa,GJ 3010,HIP1987,GJ 3076,HIP 11253,HIP 11352,and HIP 14929)are *** data were obtained at the 6 m Big Telescope Alt-azimuthal Special Astrophysical Observatory of the Russian Academy of Science(BTA SAO RAS)from 2007 to the *** of previously published and new measurements made it possible to construct completely new orbits for Chara 122Aa,HIP 11253,and HIP *** orbit of GJ 3076 cannot be constructed accurately due to the large influence of the weights assigned to the *** resulting orbital solutions are classified based on a grading scheme suggested by ***,*** and ***;most orbits are“definitive”(Grade 1).The mass sums and masses of components calculated by two independent methods using Hipparcos and Gaia DR2 and DR3 parallaxes were compared for the objects under study.

关键词： techniques:high angular resolution (stars:)binaries:visual stars:fundamental parameters stars:low-mass stars:solar-type

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Pharmacological pain management in chronic pancreatitis

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World Journal of Gastroenterology 2013年第42期19卷 7292-7301页

作者： Sφren S Olesen Jacob Juel Carina Graversen yuri Kolesnikov Oliver HG Wilder-Smith Asbjφrn m Drewes mechSense Department of Gastroenterology and HepatologyAalborg University Hospital9000 AalborgDenmark mech-Sense Department of RadiologyAalborg University Hospital9000 AalborgDenmark Department of Neurorehabilitation Engineering Bernstein Center for Computational NeuroscienceUniversity Medical Center G ttingenGeorg-August UniversityD-37077 GttingenGermany Pain Service Department of OncologyEast Tallinn Central Hospital19086 TallinnEstonia Pain and Nociception Neuroscience Research Group Department of AnaesthesiologyPain and Palliative CareRadboud University Nijmegen Medical Centre6525 NijmegenThe Netherlands Center for Sensory-motor Interaction Department of Health Science and TechnologyAalborg University9000 AalborgDenmark

Intense abdominal pain is a prominent feature of chronic pancreatitis and its treatment remains a major clinical *** studies of pancreatic nerves and experimental human pain research have provided evidence that pain processing is abnormal in these patients and in many cases resembles that seen in neuropathic and chronic pain *** important ultimate outcome of such aberrant pain processing is that once the disease has advanced and the pathophysiological processes are firmly established,the generation of pain can become self-perpetuating and independent of the initial peripheral nociceptive ***,the management of pain by traditional methods based on nociceptive deafferentation(e.g.,surgery and visceral nerve blockade)becomes difficult and often *** novel and improved understanding of pain aetiology requires a paradigm shift in pain management of chronic *** mechanism based pain treatments taking into account altered pain processing are likely to increasingly replace invasive therapies targeting the nociceptive source,which should be reserved for special and carefully selected *** this review,we offer an overview of the current available pharmacological options for pain management in chronic *** addition,future options for pain management are discussed with special emphasis on personalized pain medicine and multidisciplinarity.

关键词： Chronic pancreatitis Pain Treatment Pharmacology Analgesics Adjuvant analgesics

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mechanisms of autophagy activation in endothelial cell and their targeting during normothermic machine liver perfusion

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World Journal of Gastroenterology 2017年第48期23卷 8443-8451页

作者： yuri L Boteon Richard Laing Hynek mergental Gary m Reynolds Darius F mirza Simon C Afford Ricky H Bhogal The Liver Unit University Hospitals of BirminghamMindelsohn Way The Centre for Liver Research Institute for Biomedical ResearchUniversity of Birmingham

Ischaemia-reperfusion injury(IRI) is the leading cause of injury seen in the liver following transplantation. IRI also causes injury following liver surgery and haemodynamic shock. The first cells within the liver to be injured by IRI are the liver sinusoidal endothelial cells(LSEC). Recent evidence suggests that LSEC coordinate and regulates the livers response to a variety of injuries. It is becoming increasingly apparent that the cyto-protective cellular process of autophagy is a key regulator of IRI. In particular LSEC autophagy may be an essential gatekeeper to the development of IRI. The recent availability of liver perfusion devices has allowed for the therapeutic targeting of autophagy to reduce IRI. In particular normothermic machine liver perfusion(NmP-L) allow the delivery of pharmacological agents to donor livers whilst maintaining physiological temperature and hepatic flow rates. In this review we summarise the current understanding of endothelial autophagy and how this may be manipulated during NmP-L to reduce liver IRI.

关键词： Autophagy Liver transplant Ischaemiareperfusion injury Normothermic machine liver perfusion

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Fracture network characterisation of the Balmuccia peridotite using drone-based photogrammetry,implications for active-seismic site survey for scientific drilling

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Journal of Rock mechanics and Geotechnical Engineering 2024年第10期16卷 3961-3981页

作者： Niccolo menegoni yuri Panara Andrew Greenwood Davide mariani Alberto Zanetti Gyorgy Hetenyi Ali I.Al-Naimi Petroleum Engineering Research Center King Abdullah University of Science and TechnologyThuwal23955-6900Saudi Arabia Chair of Applied Geophysics Montanuniversit€at LeobenLeobenAustria Department of Earth and Environmental Sciences University of PaviaPaviaItaly Institute of Geosciences and Earth Resources National Research Council(IGG-CNR)PaviaItaly Institute of Earth Sciences University of Lausanne1015LausanneSwitzerland

The presence of discontinuities(***,fractures,veins,layering)in crystalline rocks can be challenging for seismic interpretations because the wide range of their size,orientation,and intensity,which controls the mechanical properties of the rock and elastic wave propagation,resulting in equally varying seismic responses at different *** geometrical characterisation of adjacent outcrop discontinuity networks allows a better understanding of the nature of the subsurface rocks and aids seismic *** this study,we characterise the discontinuity network of the Balmuccia peridotite(BP)in the IvreaeVerbano Zone(IVZ),northwestern *** geological body is the focus of the Drilling the Ivrea eVerbano zonE(DIVE),an international continental scientific drilling project,and two active seismic surveys,SEismic imaging of the Ivrea ZonE(SEIZE)and high-resolution SEIZE(Hi-SEIZE),which aim to resolve the subsurface structure of the DIVE drilling target through high-resolution seismic *** fracture characterisation,we developed two drone-based digital outcrop models(DOms)at two different resolutions(10^(-3)-10 m and 10^(-1)-10^(3)m),which allowed us to quantitatively characterise the orientation,size,and intensity of the main rock *** properties affect the seismic velocity and consequently the interpretation of the seismic *** found that(i)the outcropping BP discontinuity network is represented by three more sets of fractures with respect to those reported in the literature;(ii)the discontinuity sizes follow a power-law distribution,indicating similarity across scales,and(iii)discontinuity intensity is not uniformly distributed along the *** results help to explain the seismic behaviour of the BP detected by the SEIZE survey,suggesting that the low P-wave velocities observed can be related to the discontinuity network,and provide the basic topological parameters(orientation,density,distribution,and aperture)of the frac

关键词： Remote sensing Fracture intensity Digital outcrop model(DOm) Rock discontinuity Fault SEismic imaging of the Ivrea ZonE(SEIZE) IvreaeVerbano Zone(IVZ) Crystalline rock

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Numerical Simulation of Blood Flow in Aorta with Dilation:A Comparison between Laminar and LES modeling methods

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Computer modeling in Engineering & Sciences 2020年第8期124卷 509-526页

作者： Lijian Xu Tianyang Yang Lekang Yin Ye Kong yuri Vassilevski Fuyou Liang School of Naval Architecture Ocean and Civil EngineeringShanghai Jiao Tong UniversityShanghai200240China Shanghai Chest Hospital Shanghai Jiao Tong UniversityShanghai200030China Zhongshan Hospital Fudan UniversityShanghai200032China marchuk Institute of Numerical mathematics Russian Academy of SciencesMoscow119333Russia Institute for Personalized medicine Sechenov UniversityMoscow119991Russia Key Laboratory of Hydrodynamics(ministry of Education) Shanghai Jiao Tong UniversityShanghai200240China

Computational modeling methods have been increasingly employed to quantify aortic hemodynamic parameters that are challenging to in vivo measurements but important for the diagnosis/treatment of aortic *** the presence of turbulence-like behaviors of blood flow in normal or diseased aorta has long been confirmed,the majority of existing computational model studies adopted the laminar flow assumption(LFA)in the treatment of sub-grid flow *** far,it remains unclear whether LFA would significantly compromise the reliability of hemodynamic *** the present study,we addressed the issue in the context of a specific aortopathy,namely aortic dilation,which is usually accompanied by disturbed flow *** patient-specific aortas with treated/untreated dilation of the ascending segment were investigated,and their geometrical models were reconstructed from computed tomography angiographic images,with the boundary conditions being prescribed based on flow velocity information measured in vivo with the phase contrast magnetic resonance imaging *** the modeling of blood flow,apart from the traditional LFA-based method in which sub-grid flow dynamics is ignored,the large eddy simulation(LES)method capable of incorporating the dissipative energy loss induced by turbulent eddies at the sub-grid level,was adopted and taken as a reference for examining the performance of the LFA-based *** results showed that the simulated large-scale flow patterns with the two methods had high similarity,both agreeing well with in vivo measurements,although locally large between-method discrepancies in computed hemodynamic quantities existed in regions with high intensity of flow ***,a switch from the LES to the LFAbased modeling method led to mild(mputed space-averaged wall shear stress metrics(i.e.,SA-TAWSS,SA-OSI)in the ascending aortic segment where intensive vortex evolution accompanied by high stat

关键词： Blood flow aortic dilation computational modeling turbulence laminar flow assumption large eddy simulation

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Individual stages of bacterial dichloromethane degradation mapped by carbon and chlorine stable isotope analysis

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Journal of Environmental Sciences 2019年第4期31卷 147-160页

作者： maria L.Torgonskaya Anatoly m.Zyakun yuri A.Trotsenko Kestutis S.Laurinavichius Steffen Kümmel Stéphane Vuilleumier Hans H.Richnow Laboratory of Radioactive Isotopes G.K.Skryabin Institute of Biochemistry and Physiology of MicroorganismsFRC Pushchino Center for Biological ResearchRussian Academy of Sciences Laboratory of mass Spectrometry G.K.Skryabin Institute of Biochemistry and Physiology of MicroorganismsFRC Pushchino Center for Biological ResearchRussian Academy of Sciences Department of Isotope Biogeochemistry Helmholtz Centre for Environmental Research-UFZ Université de Strasbourg CNRS

The fractionation of carbon and chlorine stable isotopes of dichloromethane(CH_2Cl_2,DCm)upon dechlorination by cells of the aerobic methylotroph methylobacterium extorquens Dm4 and by purified DCm dehalogenases of the glutathione S-transferase family was *** effects for individual steps of the multi-stage DCm degradation process,including transfer across the cell wall from the aqueous medium to the cell cytoplasm,dehalogenase binding,and catalytic reaction,were *** observed carbon and chlorine isotope fractionation accompanying DCm consumption by cell supensions and enzymes was mainly determined by the breaking of C\Cl bonds,and not by inflow of DCm into *** isotope effects of DCm dehalogenation were initially masked in high density cultures,presumably due to inverse isotope effects of non-specific DCm oxidation under conditions of oxygen *** cofactor supply remarkably affected the correlation of variations of DCm carbon and chlorine stable isotopes(Δδ^(13)C/Δδ^(37)Cl),increasing corresponding ratio from 7.2–8.6 to 9.6–10.5 under conditions of glutathione *** suggests that enzymatic reaction of DCm with glutathione thiolate may involve stepwise breaking and making of bonds with the carbon atom of DCm,unlike the uncatalyzed reaction,which is a one-stage process,as shown by quantum-chemical modeling.

关键词： Dichloromethane(DCm) microbial degradation Aerobic methylotrophic bacteria Dichloromethane dehalogenase Carbon and chlorine isotopic fractionation

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An Experimental Prototype of an Innovative Fluid-driven Electromagnetic Stirring Technique

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Journal of Iron and Steel Research International 2016年第5期23卷 422-427页

作者： Bo WANG Xiao-dong WANG yuri KOLESNIKOV San ZHANG Yan-qing TAN Xian-zhao NA College of materials Science and Opto-electronic Technology University of Chinese Academy of Sciences Institute of Thermodynamics and Fluid mechanics Ilmenau University of Technology State Key Laboratory of Advanced Steel Processing and Products Central Iron and Steel Research Institute

A new electromagnetic stirring technique that is driven by hydrodynamic forces was presented. This technique offers the following advantages. First,the stirrer can be immersed in the liquid metal,thereby significantly increasing the penetration depth of the electromagnetic forces and significantly improving the stirring efficiency; thus,this technique is particularly suitable for large-scale liquid metal. Second,under certain conditions,this technique can overcome difficulties that are encountered with traditional stirrers,such as accessing regions that are difficult to reach in working spaces with complex or narrow shapes. This stirrer also has a simpler structure than a traditional stirrer; thus,the design can be easily modified,and no external power supply is required. An experimental prototype was also presented for controlling the fluid flow rate,thereby controlling the electromagnetic force and velocity field of the driven liquid metal. The velocity distribution in a liquid Ga In Sn alloy under fluid-driven electromagnetic stirring was quantitatively measured using ultrasonic Doppler velocimetry（ UDV）. The primary results show that a remarkable velocity field has been achieved and that fluid-driven electromagnetic stirring is an effective means of stirring liquid metal. Finally,the potential applications of this technique in industry,along with key challenges,were discussed.

关键词： fluid electromagnetic stirring liquid metal skin effect Lorentz force

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Twenty-three new spider species(Arachnida:Araneae)from Asia

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Zoological Systematics 2021年第2期46卷 91-152页

作者： Yejie Lin yuri m.marusik Caixia Gao Hao Xu Xiaoqing Zhang Ziyi Wang Wenhui Zhu Shuqiang Li Institute of Zoology Chinese Academy of SciencesBeijing 100101China Institute for Biological Problems of the North RAS Portovaya Str.18Magadan 685000Russia Department of Zoology&Entomology University of the Free StateBloemfontein 9300South Africa

A new genus,Sinophaena Lin&Li,***.,of the spider family Anyphaenidae Bertkau,1878 is reported,with *** Lin&Li,***.(♀)from Sichuan,China as the type *** bivalva(Zhang&Song,2004)*** transferred from Anyphaena Sundevall,*** new species reported in the current paper include:Anyphaena grovyle Lin&Li,***.(♂♀,Hainan),*** Lin&Li,***.(♂♀,Hainan),*** Lin&Li,***.(♂♀,Guangdong),*** Lin&Li,***.(♂♀,Tibet),*** Lin&Li,***.(♂♀,Hainan),Cybaeus fushun Lin&Li,***.(♀,Liaoning),*** Lin&Li,***.(♀,Jilin),Eresus lishizheni Lin,marusik&Li,***.(♂,Xinjiang),Alistra pikachu Lin&Li,***.(♂♀,Guangdong),Sinopesa gollum Lin&Li,***.(♂,Guangdong),Pimoa gagna Zhang&Li,***.(♀,India),*** Zhang&Li,***.(♂♀,India),*** Zhang&Li,***.(♂♀,India),Selenops crewsae Lin&Li,***.(♀,Sichuan),Sinopoda helii Wang&Li,***.(♂♀,Sichuan),Phlogiellus jiaxiangi Lin&Li,***.(♂♀,Guangxi),Episinus bonjovi Lin&Li,***.(♂♀,Yunnan),*** Lin&Li,***.(♂♀,Yunnan),*** Lin&Li,***.(♂♀,Yunnan),meotipa luoqiae Lin&Li,***.(♂,Yunnan),*** Lin&Li,***.(♂,Yunnan),and *** Lin&Li,***.(♂,Yunnan).The female of Belisana yuexiu Yao&Li,2020 is reported for the first *** types are deposited in the Institute of Zoology,Chinese Academy of Sciences(IZCAS)in Beijing,China.

关键词： Diagnosis distribution epigyne taxonomy type species

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Dynamic failure of dry and fully saturated limestone samples based on incubation time concept

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Journal of Rock mechanics and Geotechnical Engineering 2017年第1期9卷 125-134页

作者： yuri V. Petrov Ivan V. Smirnov Grigory A. Volkov Andrei K. Abramian Anatoliy m. Bragov Stanislav N. Verichev Saint Petersburg University Universitetskaya nab. 7-9 Saint Petersburg 199034 Russia Institute of Problems of mechanical Engineering Russian Academy of Sciences Bolshoj pr. 61 V.O. Saint Petersburg 199178 Russia Lobachevsky State University of Nizhni Novgorod Prospekt Gagarina 23 Nizhny Novgorod 603950 Russia Allseas Engineering B.V. Poortweg 12 Delft 2612 PA The Netherlands

This paper outlines the results of experimental study of the dynamic rock failure based on the comparison of dry and saturated limestone samples obtained during the dynamic compression and split tests. The tests were performed using the Kolsky method and its modifications for dynamic splitting. The mechanical data(e.g. strength, time and energy characteristics) of this material at high strain rates are obtained. It is shown that these characteristics are sensitive to the strain rate. A unified interpretation of these rate effects, based on the structuraletemporal approach, is hereby presented. It is demonstrated that the temporal dependence of the dynamic compressive and split tensile strengths of dry and saturated limestone samples can be predicted by the incubation time criterion. Previously discovered possibilities to optimize(minimize) the energy input for the failure process is discussed in connection with industrial rock failure processes. It is shown that the optimal energy input value associated with critical load, which is required to initialize failure in the rock media, strongly depends on the incubation time and the impact duration. The optimal load shapes, which minimize the momentum for a single failure impact, are demonstrated. Through this investigation, a possible approach to reduce the specific energy required for rock cutting by means of high-frequency vibrations is also discussed.

关键词： Dynamic strength Incubation time criterion Split Hopkinson pressure bar(SHPB) test Tensile strength Compressive strength Water-saturated limestone Vibration-assisted rock cutting

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