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Liposome trade-off strategy in mitochondria-targeted NIR-cyanine: balancing blood circulation and cell retention for enhanced antitumor phototherapy in vivo

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Nano Research 2021年第7期14卷 2432-2440页

作者： xianghan Zhang Sumei Zhao Zhiqing Gao Jialin Zhou yuqiong xia Jie Tian Changhong Shi Zhongliang Wang Engineering Research Center of Molecular&Neuroimaging Ministry of EducationSchool of Life Science and TechnologyXidian UniversityXi’an 710026China Key Laboratory of Molecular Imaging Institute of AutomationChinese Academy of SciencesBeijing 100190China Laboratory Animal Center Fourth Military Medical UniversityXi’an 710032China

Cancer phototheranostics involving optical imaging-guided photodynamic therapy(PDT)and photothermal therapy(PTT)is a localized noninvasive approach in treating ***-targeted near-infrared(NIR)cyanines are excellent therapeutic photosensitizers of ***,most mitochondria-targeted cyanines exist in the form of hydrophobic structures,which in vivo may cause cyanine aggregation during blood circulation,resulting in poor biocompatibility and limited therapeutic ***,we developed a trade-off strategy by encapsulating mitochondria-targeted cyanines into liposomal bilayers(CyBI7-LPs),which balanced hydrophilicity that favored blood circulation and hydrophobicity that enhanced mitochondria tumor ***,CyBI7-LPs greatly minimized photobleaching of cyanine as self-generated reactive oxygen species(ROS)could rapidly escape from the liposomal bilayer,affording enhanced PTT/PDT ***-mediated targeting strategy was further employed to improve uptake of tumor cells by modifying the liposomal surface to generate *** CyBI7-LPB probe produced a tumor-to-background ratio(TBR)of approximately 6.4 at 24 *** by highly sensitive imaging resulted in excellent anti-tumor therapy outcomes using CyBI7-LPB due to the enhanced photothermal and photodynamic *** proposed liposomal nanoplatform exhibited a simple and robust approach as an imaging-guided synergistic anti-tumor therapeutic strategy.

关键词： cancer phototheranostics NIR cyanine liposome mitochondria-targeted phototherapy

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《中国好声音》中导师身份建构的语用研究

云南农业大学学报（社会科学版） 2016年第2期10卷 98-103页

作者：夏玉琼金陵科技学院外国语学院江苏南京210038

基于身份建构论与身份的"自我方位模式",以《中国好声音》中导师与学员的互动话语为语料,考察了导师在节目中建构的身份类型、建构策略以及身份建构的社交—语用理据。研究发现,导师在《中国好声音》的舞台上,利用不同的言语行为与语用... 详细信息

基于身份建构论与身份的"自我方位模式",以《中国好声音》中导师与学员的互动话语为语料,考察了导师在节目中建构的身份类型、建构策略以及身份建构的社交—语用理据。研究发现,导师在《中国好声音》的舞台上,利用不同的言语行为与语用策略,分别建构了四种身份类型:专业的导师、亲和的导师、谦虚的导师和幽默的导师。身份建构是导师为满足交际需要而可供调用的语用资源。专业的导师身份和幽默的导师身份维护了导师的自我面子,而亲和的导师身份和谦虚的导师身份则维护了学员的面子。总而言之,四种身份类型都构建了导师积极正面的自我形象,实现了正能量的传播。

关键词：《中国好声音》导师身份

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冲突性话语中的身份建构研究

山东理工大学学报（社会科学版） 2015年第5期31卷 44-48页

作者：夏玉琼金陵科技学院外国语学院江苏南京210038

语言不仅传递信息,还可以建构交际双方的身份。以冲突性话语中交际者的身份为分析对象,基于Tracy&Robles对日常谈话者身份的划分,从主要身份、交往身份和个人身份或关系身份三个维度考察了交际者身份的建构,发现在冲突性话语中,为达到... 详细信息

语言不仅传递信息,还可以建构交际双方的身份。以冲突性话语中交际者的身份为分析对象,基于Tracy&Robles对日常谈话者身份的划分,从主要身份、交往身份和个人身份或关系身份三个维度考察了交际者身份的建构,发现在冲突性话语中,为达到批评、指责对方等交际目的,交际一方会有意建构或突显另一方消极、负面的身份,进而引发或加剧冲突,对和谐人际关系产生解构作用。

关键词：身份建构冲突性话语人际关系

优质护理对口腔颌面部间隙感染患者的效果分析

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临床医学工程 2018年第5期25卷 663-664页

作者：梁翠芬刘育琼何琴夏璀深圳市龙华区中心医院口腔科广东深圳518110

目的分析优质护理在口腔颌面部间隙感染患者中的临床效果。方法选取我院2015年3月至2017年2月收治的62例口腔颌面部间隙感染患者,随机分为优质组和对照组,各31例。对照组采用常规护理,优质组在对照组基础上采用优质护理。比较两组患者... 详细信息

目的分析优质护理在口腔颌面部间隙感染患者中的临床效果。方法选取我院2015年3月至2017年2月收治的62例口腔颌面部间隙感染患者,随机分为优质组和对照组,各31例。对照组采用常规护理,优质组在对照组基础上采用优质护理。比较两组患者的不良情绪改善情况、并发症发生率及护理满意度情况。结果护理后,优质组的SAS、SDS评分均低于护理前及对照组,差异有统计学意义(P<0.05)。优质组的并发症发生率为6.45%,明显低于对照组的25.81%(P<0.05)。优质组的护理满意度为90.32%,明显高于对照组的64.52%(P<0.05)。结论对口腔颌面部间隙感染患者实施优质护理,可改善患者的不良情绪,降低并发症发生率,并提高护理满意度,值得临床推广。

关键词：口腔颌面部间隙感染优质护理效果

Varicella-zoster virus ORF7 interacts with ORF53 and plays a role in its trans-Golgi network localization

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Virologica Sinica 2017年第5期32卷 387-395页

作者： Wei Wang Wenkun Fu Dequan Pan Linli Cai Jianghui Ye Jian Liu Che Liu yuqiong Que Ningshao xia Hua Zhu Tong Cheng State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics National Institute of Diagnostics and Vaccine Development in Infectious Diseases School of Public Health Xiamen University Department of Microbiology Biochemistry and Molecular Genetics New Jersey Medical SchoolRutgers University

Varicella-zoster virus(VZV) is a neurotropic alphaherpesvirus that causes chickenpox and shingles. ORF7 is an important virulence determinant of VZV in both human skin and nerve tissues,however, its specific function and involved molecular mechanism in VZV pathogenesis remain largely elusive. Previous yeast two-hybrid studies on intraviral protein-protein interaction network in herpesviruses have revealed that VZV ORF7 may interact with ORF53, which is a virtually unstudied but essential viral protein. The aim of this study is to identify and characterize VZV ORF53, and to investigate its relationship with ORF7. For this purpose, we prepared monoclonal antibodies against ORF53 and, for the first time, characterized it as a ~40 k Da viral protein predominantly localizing to the trans-Golgi network of the infected host cell. Next, we further confirmed the interaction between ORF7 and ORF53 by co-immunoprecipitation and co-localization studies in both plasmid-transfected and VZV-infected cells. Moreover, interestingly, we found that ORF53 lost its trans-Golgi network localization and became dispersed in the cytoplasm of host cells infected with an ORF7-deleted recombinant VZV, and thus ORF7 seems to play a role in normal subcellular localization of ORF53. Collectively, these results suggested that ORF7 and ORF53 may function as a complex during infection, which may be implicated in VZV pathogenesis.

关键词： varicella-zoster virus(VZV) ORF7 ORF53 protein-protein interaction trans-Golgi network

Combination therapy with oncolytic virus and T cells or mRNA vaccine amplifies antitumor effects

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Signal Transduction and Targeted Therapy 2024年第6期9卷 2655-2668页

作者： Rao Fu Ruoyao Qi Hualong Xiong Xing Lei Yao Jiang Jinhang He Feng Chen Liang Zhang Dekui Qiu Yiyi Chen Meifeng Nie Xueran Guo Yuhe Zhu Jinlei Zhang Mingxi Yue Jiali Cao Guosong Wang yuqiong Que Mujing Fang Yingbin Wang Yixin Chen Tong Cheng Shengxiang Ge Jun Zhang Quan Yuan Tianying Zhang Ningshao xia State Key Laboratory of Vaccines for Infectious Diseases Xiang An Biomedicine LaboratoryDepartment of Laboratory MedicineSchool of Public Health&School of Life SciencesXiamen UniversityXiamen 361102 FujianChina National Institute of Diagnostics and Vaccine Development in Infectious Diseases Xiamen 361102 FujianChina

Antitumor therapies based on adoptively transferred T cells or oncolytic viruses have made significant progress in recent years,but the limited efficiency of their infiltration into solid tumors makes it difficult to achieve desired antitumor effects when used *** this study,an oncolytic virus(rVSV-LCMVG)that is not prone to induce virus-neutralizing antibodies was designed and combined with adoptively transferred T *** transforming the immunosuppressive tumor microenvironment into an immunosensitive one,in B16 tumor-bearing mice,combination therapy showed superior antitumor effects than *** occurred whether the OV was administered intratumorally or *** therapy significantly increased cytokine and chemokine levels within tumors and recruited CD8^(+)T cells to the TME to trigger antitumor immune *** with adoptively transferred T cells and subsequent oncolytic virotherapy sensitizes refractory tumors by boosting T-cell recruitment,downregulating the expression of PD-1,and restoring effector T-cell *** offer a combination therapy with greater translational value,mRNA vaccines were introduced to induce tumor-specific T cells instead of adoptively transferred T *** combination of OVs and mRNA vaccine also displays a significant reduction in tumor burden and prolonged *** study proposed a rational combination therapy of OVs with adoptive T-cell transfer or mRNA vaccines encoding tumor-associated antigens,in terms of synergistic efficacy and mechanism.

关键词： transferred rational boosting

一种简易同时蒸馏萃取方法用于八角茴香挥发油提取及其GC-MS分析

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应用化学 2015年第3期32卷 356-361页

作者：孙玉琼夏东海董新荣李霞湖南农业大学理学院长沙410128

设计了一种简易同时蒸馏萃取(SSDE)装置并用于八角茴香挥发油提取及其气相色谱-质谱法(GCMS)分析。该装置由圆底烧瓶、恒压滴液漏斗和回流冷凝管构成。通过对八角茴香挥发油组分的GC-MS分析,比较SSDE法和水蒸汽蒸馏提取法(HD)提取植物... 详细信息

设计了一种简易同时蒸馏萃取(SSDE)装置并用于八角茴香挥发油提取及其气相色谱-质谱法(GCMS)分析。该装置由圆底烧瓶、恒压滴液漏斗和回流冷凝管构成。通过对八角茴香挥发油组分的GC-MS分析,比较SSDE法和水蒸汽蒸馏提取法(HD)提取植物源挥发油的效果。结果表明,SSDE法所得挥发油相对含量在0.1%以上的19种化合物占挥发油总量的98.84%(相对标准偏差(RSD)(n=3)均不大于5.59%,其中RSD﹤5%的17种化合物占鉴定组分89.5%。SSDE法和HD法挥发油平均收率分别为7.30%(RSD=2.37%(n=5))和6.67%(RSD=5.26%(n=5))。SSDE法简便、快速,适用于八角茴香挥发油的提取。

关键词：同时蒸馏萃取水蒸汽蒸馏气相色谱/质谱挥发油八角茴香

Two antibodies show broad,synergistic neutralization against SARS-CoV-2 variants by inducing conformational change within the RBD

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Protein & Cell 2024年第2期15卷 121-134页

作者： Hui Sun Tingting Deng Yali Zhang Yanling Lin Yanan Jiang Yichao Jiang Yang Huang Shuo Song Lingyan Cui Tingting Li Hualong Xiong Miaolin Lan Liqin Liu Yu Li Qianjiao Fang Kunyu Yu Wenling Jiang Lizhi Zhou yuqiong Que Tianying Zhang Quan Yuan Tong Cheng Zheng Zhang Hai Yu Jun Zhang Wenxin Luo Shaowei Li Qingbing Zheng Ying Gu Ningshao xia State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics School of Public HealthSchool of Life SciencesXiamen UniversityXiamen 361102China National Institute of Diagnostics and Vaccine Development in Infectious Diseases School of Public HealthSchool of Life SciencesXiamen UniversityXiamen 361102China State Key Laboratory of Vaccines for Infectious Diseases School of Public HealthSchool of Life SciencesXiamen UniversityXiamen 361102China xiang An Biomedicine Laboratory Xiamen 361102China Institute for Hepatology National Clinical Research Center for Infectious DiseaseShenzhen Third People's HospitalSchool of MedicineSouthem University of Science and TechnologyShenzhen 518112China The Second Affliated Hospital School of MedicineSouthern University of Science and TechnologyShenzhen 518112China Research Unit of Frontier Technology of Structural Vaccinology Chinese Academy of Medical SciencesXiamen 361102China

Continual evolution of the severe acute respiratory syndrome coronavirus(SARS-CoV-2)virus has allowed for its gradual evasion of neutralizing antibodies(nAbs)produced in response to natural infection or *** rapid nature of these changes has incited a need for the development of superior broad nAbs(bnAbs)and/or the rational design of an antibody cocktail that can protect against the mutated virus ***,we report two angiotensin-converting enzyme 2 competing nAbs—8H12 and 3E2—with synergistic neutralization but evaded by some Omicron ***-electron microscopy reveals the two nAbs synergistic neutralizing virus through a rigorous pairing permitted by rearrangement of the 472-489 loop in the receptor-binding domain to avoid steric *** antibodies based on these two nAbs tremendously extend the neutralizing breadth and restore neutralization against recent variants including currently dominant ***,these findings expand our understanding of the potential strategies for the neutralization of SARS-CoV-2 variants toward the design of broad-acting antibody therapeutics and vaccines.

关键词： SARS-CoV-2 broad neutralizing antibody rearrangement synergistic neutralization

轻质高导电金属化碳纳米管薄膜的制备及其雷击防护性能

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复合材料学报 2024年第1期41卷 196-206页

作者：席佳琦戴亚光夏雷王玉琼杨文刚吕卫帮上海大学材料科学与工程学院上海200444 沈阳飞机设计研究所沈阳110035 中国科学院苏州纳米技术与纳米仿生研究所先进纳米复合材料创新中心苏州215123

碳纤维增强树脂基复合材料(CFRP)导电性差,无法满足飞机航行过程中的雷击防护需求。金属化碳纳米管薄膜具备轻质、高导电、高载流的特点,可应用于复合材料雷击防护。采用电化学沉积工艺成功制备了一种碳纳米管(CNT)/Cu复合薄膜,并对其... 详细信息

碳纤维增强树脂基复合材料(CFRP)导电性差,无法满足飞机航行过程中的雷击防护需求。金属化碳纳米管薄膜具备轻质、高导电、高载流的特点,可应用于复合材料雷击防护。采用电化学沉积工艺成功制备了一种碳纳米管(CNT)/Cu复合薄膜,并对其微观结构、电学性能及载流失效行为进行了表征分析。结果表明,CNT/Cu复合薄膜柔性较好,具有明显的梯度结构,铜的含量在薄膜厚度方向上逐渐递减。复合薄膜电导率与Cu同一量级,比电导率为Cu的2倍,载流量及比载流量分别为商用铜网的1.4倍和7倍。复合薄膜中的CNT抑制了Cu在大载流作用下电迁移的发生,进而延长其载流失效时间。基于CNT/Cu复合薄膜制备了CFRP雷击防护试样,进行人工模拟雷击试验及高精度损伤分析,评估雷击防护效果。与商用铜网雷击防护材料相比,CNT/Cu复合薄膜质量减轻61%,且表现出更为优异的雷击防护性能。

关键词： CNT/Cu 复合材料电学性能金属化雷击防护无损分析碳纤维增强树脂基