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An ethylene-induced NAC transcription factor acts as a multiple abiotic stress responsor in conifer

Horticulture Research 2023年第8期10卷 158-169页

作者： Fangxu Han Peiyi Wang Xi Chen Huanhuan Zhao Qianya Zhu Yitong Song yumeng nie Yue Li Meina Guo Shihui Niu National Engineering Research Center of Tree Breeding and Ecological Restoration College of Biological Sciences and TechnologyBeijing Forestry UniversityBeijing 100083China

The proper response to various abiotic stresses is essential for plants’survival to overcome their sessile nature,especially for perennial trees with very long-life ***,in conifers,the molecular mechanisms that coordinate multiple abiotic stress responses remain ***,the transcriptome response to various abiotic stresses like salt,cold,drought,heat shock and osmotic were systematically detected in Pinus tabuliformis(***)*** found that four transcription factors were commonly induced by all tested stress treatments,while PtNAC3 and PtZFP30 were highly up-regulated and ***,the exogenous hormone treatment assays and the content of the endogenous hormone indicates that the upregulation of PtNAC3 and PtZFP30 are mediated by ***-course assay showed that the treatment by ethylene immediate precursor,1-aminocyclopropane-1-carboxylic acid(ACC),activated the expression of PtNAC3 and PtZFP30 within 8 *** further confirm that the PtNAC3 can directly bind to the PtZFP30 promoter region and form a *** of PtNAC3 enhanced unified abiotic stress tolerance without growth penalty in transgenic Arabidopsis and promoted reproductive success under abiotic stress by shortening the lifespan,suggesting it has great potential as a biological tool applied to plant breeding for abiotic stress *** study provides novel insights into the hub nodes of the abiotic stresses response network as well as the environmental adaptation mechanism in conifers,and provides a potential biofortification tool to enhance plant unified abiotic stress tolerance.

关键词： stress endogenous unified

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Determination of conifer age biomarker DAL1 interactome using Y2H-seq

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Forestry Research 2021年第1期1卷 106-117页

作者： Xi Chen Qianya Zhu yumeng nie Fangxu Han Yue Li Harry XWu Shihui Niu Beijing Advanced Innovation Center for Tree Breeding by Molecular Design National Engineering Laboratory for Tree BreedingCollege of Biological Sciences and TechnologyBeijing Forestry UniversityBeijing 100083PR China Umea Plant Science Centre Department of Forest Genetics and Plant PhysiologySwedish University of Agricultural SciencesLinnaeus vag 6SE-90183UmeaSweden

Age is a sophisticated physiological signal that ensures the sequence of different developmental stages in *** regulation of ageing pathways appears to differ between gymnosperms and *** previously identified DAL1 as a conserved conifer age biomarker that plays a crucial role in the transition from vegetative to reproductive life-history phases in ***,elucidating the specific interaction events related to DAL1 is key to understanding how age drives conifer ***-scale yeast two-hybrid(Y2H)analysis followed by next-generation high-throughput sequencing(Y2H-seq)allowed us to identify 135 PtDAL1 interacting proteins in Pinus *** study found that PtDAL1 interacting proteins showed an ageing-related module,with sophisticated interacting networks composed of transcription factors(TFs),transcriptional regulators(TRs),and *** interacting proteins are produced in response to a variety of phytohormones and environmental signals,and are likely involved in wood formation,needle development,oleoresin terpenoids biosynthesis,and reproductive *** this study,we propose a novel regulation model of conifer ageing pathways whereby PtDAL1 coordinates different environmental stimuli and interacts with corresponding proteins to regulate appropriate development.

关键词： development likely DAL1

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The long road to bloom in conifers

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Forestry Research 2022年第1期2卷 157-166页

作者： Jingjing Ma Xi Chen Fangxu Han Yitong Song Biao Zhou yumeng nie Yue Li Shihui Niu Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants College of Landscape and ArchitectureZhejiang Agriculture and Forestry UniversityHangzhou 311300ZhejiangPR China National Engineering Research Center of Tree Breeding and Ecological Restoration College of Biological Sciences and TechnologyBeijing Forestry UniversityBeijing 100083PR China Research Institute of Forestry Chinese Academy of ForestryBeijing 100091PR China

More than 600 species of conifers(phylum Pinophyta)serve as the backbone of the Earth’s terrestrial plant community and play key roles in global carbon and water *** coniferous forests account for a large fraction of global wood production,their productivity relies largely on the use of genetically improved ***,acquisition of such seeds requires recurrent selection and testing of genetically superior parent trees,eventually followed by the establishment of a seed orchard to produce the improved *** breeding cycle for obtaining the next generation of genetically improved seeds can be significantly lengthened when a target species has a long juvenile ***,development of methods for diminishing the juvenile phase is a cost-effective strategy for shortening breeding cycle in *** molecular regulatory programs associated with the reproductive transition and annual reproductive cycle of conifers are modulated by environmental cues and endogenous developmental *** evidence indicates that an increase in global average temperature seriously threatens plant productivity,but how conifers respond to the ever-changing natural environment has yet to be fully *** the breakthrough of assembling and annotating the giant genome of conifers,identification of key components in the regulatory cascades that control the vegetative to reproductive transition is ***,comparison of the signaling pathways that control the reproductive transition in conifers and the floral transition in Arabidopsis has revealed many ***,a more complete understanding of the underlying regulatory mechanisms that control the conifer reproductive transition is of paramount ***,we review our current understanding of the molecular basis for reproductive regulation,highlight recent discoveries,and review new approaches for molecular research on conifers.

关键词： breeding breakthrough backbone

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企业家出身背景与创新投入的关系研究

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科研管理 2023年第3期44卷 158-166页

作者：高勇强聂雨朦何晓斌华中科技大学管理学院湖北武汉430074 清华大学社会科学学院北京100084

企业家个人经历对企业的战略决策如创新投入有重要影响,然而现有研究极少关注企业家的出身或初始社会阶层。本文基于烙印理论和社会阶层领域研究文献,分析了企业家初始社会阶层对创新投入的影响。来自2014年全国民(私)营企业调查数据的... 详细信息

企业家个人经历对企业的战略决策如创新投入有重要影响,然而现有研究极少关注企业家的出身或初始社会阶层。本文基于烙印理论和社会阶层领域研究文献,分析了企业家初始社会阶层对创新投入的影响。来自2014年全国民(私)营企业调查数据的实证检验显示:相比于非农民企业家,农民企业家在创新上投入更多。此外,企业家创业前的工作经历(销售工作vs其他工作)和企业内的员工培训投入显著调节了农民企业家的创新投入。研究表明:企业家的初始社会阶层会给企业家留下与所处阶层相对应的“烙印”,塑造其风险偏好,进而影响其对创新活动的态度。

关键词：农民企业家研发投入烙印理论社会阶层

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Preparation and characterization of a new magnetic fluid with good low temperature resistance

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Nano Research 2025年第1期18卷 477-486页

作者： Shilin nie Decai Li ✉1 Department of Mechanical Engineering Tsinghua UniversityBeijing 100084China State Key Laboratory of Tribology in advanced equipment Tsinghua UniversityBeijing 100084China

Magnetic fluids are a type of novel functionalmaterial that has emerged over the past two decades, whichhave found applications in various aspects of production anddaily life. However, the application of conventional magneticfluids in low-temperature environments is severely limited andoften unsatisfactory. To address this issue, we havedeveloped a new magnetic fluid with excellent low-temperatureresistance. Initially, bare Fe_(3)O_(4) magnetic nanoparticles(FMNPs) were synthesized via co-precipitation without aprotective gas. Subsequently, these particles were modified using polyethylene glycol (PEG)-4000 as a surfactant. Thebare and modified FMNPs (MFMNPs) were characterized using X-ray diffraction (XRD), vibrating sample magnetometer(VSM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and thermogravimetricanalysis-differential thermal gravimetric (TGA-DTG) analysis. The rheological properties, low-temperature resistance of themagnetic fluid were also evaluated. The characterization results indicate that the MFMNPs are spherical andmonodisperse, with a narrow size distribution and a mean particle size of approximately 12 nm. Furthermore, the FTIRspectra and TGA-DTA results suggest that PEG-4000 is linked to the bare Fe_(3)O_(4) particles via hydrogen bonding, indicatingsuccessful modification of the Fe_(3)O_(4) magnetic nanoparticles using PEG. VSM measurements demonstrate that surfacemodification does not alter the crystal morphology or superparamagnetic of Fe_(3)O_(4). However, it does reduce the saturationmagnetization from 68.17 to 54.75 emu/g. Additionally, the prepared magnetic fluid exhibits shear thinning and magneticviscosity effects. It also exhibits excellent low-temperature resistance, maintaining good fluidity without freezing even at -60 ℃. In summary, these results collectively indicate that the new low-temperature resistant magnetic fluid developed in thisstudy is stable and has broad application prospects.

关键词： magnetic fluid low-temperature resistance polyethylene glycol ethylene glycol aqueous solution superparamagnetic

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Advances in therapies using mesenchymal stem cells and their exosomes for treatment of peripheral nerve injury:state of the art and future perspectives

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Neural Regeneration Research 2025年第11期20卷 3151-3171页

作者： Fatima Aldali Chunchu Deng Mingbo nie Hong Chen Department of Rehabilitation Medicine Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina Department of Orthopedics Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina

“Peripheral nerve injury”refers to damage or trauma affecting nerves outside the brain and spinal *** nerve injury results in movements or sensation impairments,and represents a serious public health *** severed peripheral nerves have been effectively joined and various therapies have been offered,recovery of sensory or motor functions remains limited,and efficacious therapies for complete repair of a nerve injury remain *** emerging field of mesenchymal stem cells and their exosome-based therapies hold promise for enhancing nerve regeneration and *** stem cells,as large living cells responsive to the environment,secrete various factors and *** latter are nano-sized extracellular vesicles containing bioactive molecules such as proteins,microRNA,and messenger RNA derived from parent mesenchymal stem *** have pivotal roles in cell-to-cell communication and nervous tissue function,offering solutions to changes associated with cell-based *** ongoing investigations,mesenchymal stem cells and mesenchymal stem cell-derived exosome-based therapies are in the exploratory stage.A comprehensive review of the latest preclinical experiments and clinical trials is essential for deep understanding of therapeutic strategies and for facilitating clinical *** review initially explores current investigations of mesenchymal stem cells and mesenchymal stem cell-derived exosomes in peripheral nerve injury,exploring the underlying ***,it provides an overview of the current status of mesenchymal stem cell and exosomebased therapies in clinical trials,followed by a comparative analysis of therapies utilizing mesenchymal stem cells and ***,the review addresses the limitations and challenges associated with use of mesenchymal stem cell-derived exosomes,offering potential solutions and guiding future directions.

关键词： clinical trials exosome extracellular vesicles mesenchymal stem cells nerve regeneration peripheral nerve injury pre-clinical experiments

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不同活性炭活化过硫酸盐的效能及机理的规律研究

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环境科学学报 2018年第4期38卷 1501-1508页

作者：史宸菲薛瑞杰李雨濛贾淑敏聂鹂尧张满帆王国祥南京师范大学环境学院南京210023

选取煤质活性炭(F400D)、木质活性炭(Norit)和椰壳活性炭(Youshi)作为过硫酸盐(PS)活化剂,通过动力学实验研究了不同活性炭对过硫酸盐的活化效能及机理,考察了氯化钠和碳酸钠对活性炭/过硫酸盐体系的影响.结果表明,3种活性炭/过硫酸盐... 详细信息

选取煤质活性炭(F400D)、木质活性炭(Norit)和椰壳活性炭(Youshi)作为过硫酸盐(PS)活化剂,通过动力学实验研究了不同活性炭对过硫酸盐的活化效能及机理,考察了氯化钠和碳酸钠对活性炭/过硫酸盐体系的影响.结果表明,3种活性炭/过硫酸盐体系均可实现橙黄G的高效去除,效能略有差异,依次为:F400D/PS>Norit/PS>Youshi/PS.活性炭对染料的去除包括吸附作用和活化过硫酸盐的氧化降解作用,吸附位点与活化位点在活性炭上具有不同的分布.活性炭/过硫酸盐体系对1~100 mmol·L^(-1)氯化钠有很强的耐受作用,适用于高盐废水中有机污染物的去除.1~10 mmol·L^(-1)的碳酸钠对体系有明显的抑制作用.

关键词：过硫酸盐活性炭机理染料氯化钠碳酸钠

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Biomass materials for zinc-based sustainable and green energy storage devices:Strategy and mechanism

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Nano Research 2025年第1期18卷 294-314页

作者： Xiaotong Yang Xiaoxin nie Chenke Tang Yiyang Xiao Qiongguang Li Du Yuan Meng Yao College of Materials Science and Engineering Changsha University of Science and TechnologyChangsha 410004China College of Materials Science and Engineering Sichuan UniversityChengdu 610065China Anhui Province International Research Center on Advanced Building Materials School of Materials and Chemical EngineeringAnhui Jianzhu UniversityHefei 230601China

As next-generation rechargeable alternatives,zincbased energy storage devices(ZESs)are being intensely explored due to their merits of abundant resource,low cost,safety and environmental ***,ZESs face a succession of critical challenges on pursuing advancing performance,including the stability and kinetics of cathode,stability and transport of zinc electrolyte,reversibility and deep utilization of metallic Zn ***,possessing unique molecular structures with abundant functionals groups,motivates the interdisciplinary field emerging from biomass and aqueous rechargeable *** its high compatibility with ZES design,we here summarize the application of biomass materials in ZESs from the aspects of cathode,electrolyte,membrane/separator and Zn anode,with their corresponding operational mechanisms and attractive functionalities from polymeric ***,the outlooks and perspectives are provided,regarding current challenges and future *** anticipate our minireview paves way on exploring the roles of biomass in aqueous rechargeable batteries.

关键词： zinc biomass materials skeleton/host coating solid-electrolyte interphase(SEI)

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A matrix metalloproteinase-responsive hydrogel system controls angiogenic peptide release for repair of cerebral ischemia/reperfusion injury

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Neural Regeneration Research 2025年第2期20卷 503-517页

作者： Qi Liu Jianye Xie Runxue Zhou Jin Deng Weihong nie Shuwei Sun Haiping Wang Chunying Shi Department of Human Anatomy Histology and EmbryologySchool of Basic MedicineQingdao UniversityQingdaoShandong ProvinceChina Department of Neurology The Affiliated Hospital of Qingdao UniversityQingdaoShandong ProvinceChina Department of General Practice The Affiliated Hospital of Qingdao UniversityQingdaoShandong ProvinceChina

Vascular endothelial growth factor and its mimic peptide KLTWQELYQLKYKGI(QK)are widely used as the most potent angiogenic factors for the treatment of multiple ischemic ***,conventional topical drug delivery often results in a burst release of the drug,leading to transient retention(inefficacy)and undesirable diffusion(toxicity)in ***,a drug delivery system that responds to changes in the microenvironment of tissue regeneration and controls vascular endothelial growth factor release is crucial to improve the treatment of ischemic *** metalloproteinase-2(MMP-2)is gradually upregulated after cerebral ***,vascular endothelial growth factor mimic peptide QK was self-assembled with MMP-2-cleaved peptide PLGLAG(TIMP)and customizable peptide amphiphilic(PA)molecules to construct nanofiber hydrogel ***-TIMP-QK was found to control the delivery of QK by MMP-2 upregulation after cerebral ischemia/reperfusion and had a similar biological activity with vascular endothelial growth factor in *** results indicated that PA-TIMP-QK promoted neuronal survival,restored local blood circulation,reduced blood-brain barrier permeability,and restored motor *** findings suggest that the self-assembling nanofiber hydrogel PA-TIMP-QK may provide an intelligent drug delivery system that responds to the microenvironment and promotes regeneration and repair after cerebral ischemia/reperfusion injury.

关键词： angiogenesis biomaterial blood-brain barrier cerebral ischemia/reperfusion injury control release drug delivery inflammation QK peptides matrix metalloproteinase-2 neuroprotection self-assembling nanofiber hydrogel

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The interaction between KIF21A and KANK1 regulates dendritic morphology and synapse plasticity in neurons

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Neural Regeneration Research 2025年第1期20卷 209-223页

作者： Shi-Yan Sun Lingyun nie Jing Zhang Xue Fang Hongmei Luo Chuanhai Fu Zhiyi Wei Ai-Hui Tang Hefei National Research Center for Physical Sciences at the Microscale CAS Key Laboratory of Brain Function and DiseaseMinistry of Education Key Laboratory for Membrane-less Organelles and Cellular DynamicsDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhui ProvinceChina Institute of Artificial Intelligence Hefei Comprehensive National Science CenterHefeiAnhui ProvinceChina CAS Center for Excellence in Molecular Cell Sciences Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhui ProvinceChina Department of Neurobiology School of Life SciencesSouthern University of Science and TechnologyShenzhenGuangdong ProvinceChina Brain Research Center Southern University of Science and TechnologyShenzhenGuangdong ProvinceChina

Morphological alterations in dendritic spines have been linked to changes in functional communication between neurons that affect learning and ***-4 KIF21A helps organize the microtubule-actin network at the cell cortex by interacting with KANK1;however,whether KIF21A modulates dendritic structure and function in neurons remains *** this study,we found that KIF21A was distributed in a subset of dendritic spines,and that these KIF21A-positive spines were larger and more structurally plastic than KIF21A-negative ***,the interaction between KIF21A and KANK1 was found to be critical for dendritic spine morphogenesis and synaptic *** of either KIF21A or KANK1 inhibited dendritic spine morphogenesis and dendritic branching,and these deficits were fully rescued by coexpressing full-length KIF21A or KANK1,but not by proteins with mutations disrupting direct binding between KIF21A and KANK1 or binding between KANK1 and *** down KIF21A in the hippocampus of rats inhibited the amplitudes of long-term potentiation induced by high-frequency stimulation and negatively impacted the animals’cognitive *** together,our findings demonstrate the function of KIF21A in modulating spine morphology and provide insight into its role in synaptic function.

关键词： actin cytoskeleton dendrite KANK1 KIF21A microtubule spine morphology spine synaptic plasticity talin1

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