Determination of conifer age biomarker DAL1 interactome using Y2H-seq
作者机构：Beijing Advanced Innovation Center for Tree Breeding by Molecular DesignNational Engineering Laboratory for Tree BreedingCollege of Biological Sciences and TechnologyBeijing Forestry UniversityBeijing 100083PR China Umea Plant Science CentreDepartment of Forest Genetics and Plant PhysiologySwedish University of Agricultural SciencesLinnaeus vag 6SE-90183UmeaSweden
出 版 物：《Forestry Research》 (林学研究（英文）)
年 卷 期：2021年第1卷第1期
页 面：106-117页
基 金：supported by‘The National Natural Science Foundation of China(NO.31870651)’ ‘The Fundamental Research Funds for the Central Universities(NO.2021ZY55)’
主 题：development likely DAL1
摘 要：Age is a sophisticated physiological signal that ensures the sequence of different developmental stages in *** regulation of ageing pathways appears to differ between gymnosperms and *** previously identified DAL1 as a conserved conifer age biomarker that plays a crucial role in the transition from vegetative to reproductive life-history phases in ***,elucidating the specific interaction events related to DAL1 is key to understanding how age drives conifer ***-scale yeast two-hybrid(Y2H)analysis followed by next-generation high-throughput sequencing(Y2H-seq)allowed us to identify 135 PtDAL1 interacting proteins in Pinus *** study found that PtDAL1 interacting proteins showed an ageing-related module,with sophisticated interacting networks composed of transcription factors(TFs),transcriptional regulators(TRs),and *** interacting proteins are produced in response to a variety of phytohormones and environmental signals,and are likely involved in wood formation,needle development,oleoresin terpenoids biosynthesis,and reproductive *** this study,we propose a novel regulation model of conifer ageing pathways whereby PtDAL1 coordinates different environmental stimuli and interacts with corresponding proteins to regulate appropriate development.
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