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Metabolome and RNA-seq reveal discrepant metabolism and secretory metabolism profile in skeletal muscle between obese and lean pigs at different ages

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Science China Life Sciences 2025年

作者： Liu Guo Junfei Xu Wenyue Zhou Sisi Chen Hanjing Shi Mengmeng Han Zekun Yang Yehui Duan Weijun Pang yulong yin Fengna Li Laboratory of Animal Nutritional Physiology and Metabolic Process Key Laboratory of Agro-ecological Processes in Subtropical Region Institute of Subtropical Agriculture Chinese Academy of Sciences University of Chinese Academy of Sciences Laboratory of Animal Fat Deposition and Muscle Development College of Animal Science and Technology Northwest A&F University College of Advanced Agricultural Sciences University of Chinese Academy of Sciences

Metabolites and metabolism-related gene expression profiles in skeletal muscle change dramatically under obesity, aging and metabolic disease. Since obese and lean pigs are ideal models for metabolic research. Here, we compared metabolome and transcriptome of Longissimus dorsi(LD) muscle between Taoyuan black(TB, obese) and Duroc(lean) pigs at different ages. We defined the “window phase” of intramuscular fat(IMF) deposition in TB pig, which has significantly higher IMF than Duroc pig. Our results displayed discrepant lipid composition and different expression genes(DEGs) enriched in lipid metabolism, and both metabolome and transcriptome analyses revealed stronger energy expenditure and more active amino acid and protein metabolism in Duroc pig. 10 up-and 51 down-regulated biomarker metabolites with age-and breed-specificity were identified. Potential secretory metabolites, including organic acid(fumaric acid, succinate,malic acid, and gamma-aminobutyric acid), amino acid(L-lysine, and L-glutamic acid), lipid(2-hydroxyisovaleric acid, and L-carnitine) were demonstrated a significant correlation with IMF deposition. Our research highlights the huge difference of metabolic spectrum in skeletal muscle between obese and lean model and muscle-derived secretory metabolites might act as an ambassador of intercellular communication to regulate systematic metabolism.

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Trace elements in nutrition and health:a deep dive into essentiality and mechanism of their biological roles

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Science China(Life Sciences) 2023年第9期66卷 1949-1951页

作者： Dan Wan yulong yin Laboratory of Animal Nutritional Physiology and Metabolic Process Key Laboratory of Agro-ecological Processes in Subtropical RegionInstitute of Subtropical AgricultureChinese Academy of SciencesChangsha 410125China University of Chinese Academy of Sciences Beijing 101408China

Although trace elements are present in various organisms at levels lower than 1/10,000 of their mass,they are essential for survival and health of *** play crucial roles in a variety of biological processes,including DNA replication,transcription,cell growth,and cofactors of various *** animals,trace elements such as iron(Fe),copper(Cu),zinc(Zn),manganese(Mn),and selenium(Se)are mainly ingested from commercial feeds and functional feed *** ingested,these elements are released into the stomach and absorbed in the proximal small ***,they enter systemic circulation through the portal vein and are transported to other *** distribution of trace elements and their functions,particularly with respect to the intestinal and gut microbiota,are of vital ***,we are publishing this special issue to highlight novel scientific findings and field applications of trace element nutrition in China.

关键词： elements mechanism manganese

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Amino acids in piglet diarrhea:Effects,mechanisms and insights

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Animal Nutrition 2024年第1期16卷 267-274页

作者： Xihong Zhou Jing Liang Xia Xiong yulong yin Key Laboratory of Agro-ecological Processes in Subtropical Region Institute of Subtropical AgricultureThe Chinese Academy of SciencesChangsha 410125China College of Advanced Agricultural Sciences University of Chinese Academy of SciencesBeijing 100049China

Piglet diarrhea is among one of the most serious health problems faced by the pig industry,resulting in significant economic *** disease in piglets has a multifactorial etiology that is affected by physiology,environment,and management *** is the most apparent symptom of intestinal *** a key class of essential nutrients in the piglet diet,amino acids confer a variety of beneficial effects on piglets in addition to being used as a substrate for protein synthesis,including maintaining appropriate intestinal integrity,permeability and epithelial renewal,and alleviating morphological damage and inflammatory and oxidative ***,provision of appropriate levels of amino acids could alleviate piglet *** amino acid effects are mediated by metabolites,gut microbes,and related signaling *** this review,we summarize the current understanding of dietary amino acid effects on gut health and diarrhea incidence in piglets,and reveal the mechanisms *** also provide ideas for using amino acid blends and emphasize the importance of amino acid balance in the diet to prevent diarrhea in piglets.

关键词： Piglet Amino acid Diarrhea Gut microbe Immune

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The initiative for consensus and standards in pig-to-human xenotransplantation in China

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The Innovation 2024年第6期5卷 3-4页

作者： Dong Niu yulong yin Yuelushan Laboratory Changsha 410128China Key Laboratory of Livestock and Poultry Resources(Pig)Evaluation and Utilization Ministry of Agriculture and Rural AffairsCollege of Animal Science and TechnologyHunan Agricultural UniversityChangsha 410128China Institute of Subtropical Agriculture Chinese Academy of SciencesChangsha 410125China College of Animal Science and Technology Zhejiang A&F UniversityHangzhou 311300China Nanjing Outstanding Gene Technology Co. Ltd.Nanjing 210018China

The global organ shortage is critically severe,withmany patients at risk of lifethreatening conditions due to the scarcity of suitable donor ***-edited porcine organs for human transplantation have emerged as a promising solution and a focus of scientific *** urge all relevant parties in China to collaborate in establishing consensus and standards for xenotransplantation using genetically modified pigs to benefit patients worldwide.

关键词： organs establishing xen

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Extraction and identification of the chyme proteins in the digestive tract of growing pigs

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Science China(Life Sciences) 2018年第11期61卷 1396-1406页

作者： Jianjun Li Bie Tan yulong Tang Peng Liao Kang Yao Peng Ji yulong yin National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production Key Laboratory of Agro-ecological Processes in Subtropical RegionInstitute of Subtropical AgricultureChinese Academy of SciencesChangsha 410125China Department of Nutrition University of California DavisDavis 95616USA

This study aimed to explore the rule of degradation of dietary proteins by identifying chyme proteins in different segments of the digestive tract of growing pigs, using proteomics techniques. Six growing pigs were fed a corn-soybean meal-based diet for 7 days. The feedstuff and chyme proteins were separately extracted and separated with SDS-PAGE. 2 D LCMS/MS combined with protein database searching identified 1,513 proteins in different segments of the gastrointestinal tract, the number of identified exogenous proteins gradually decline from the stomach to colon, with large amounts in the duodenum to the large *** corn proteins than soybean proteins were identified both in the feedstuff and chyme, and these were significantly decreased after digestion in the stomach. More membrane proteins than non-membrane proteins were identified in whole digestive *** results regarding the profiles of chyme proteins in different segments of the gastrointestinal tract would provide useful information for optimizing feed formula in pigs.

关键词： protein profiles degradation law proteomics digestive tract growing pigs

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Paternal Zn-deficiency abolishes metabolic effects in offspring induced by diet type

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Animal Nutrition 2022年第1期8卷 310-320页

作者： Guanya Li Zhenglin Dong Shusheng Yue Dan Wan yulong yin Key Laboratory of Agro-Ecological Processess in Subtropical Region Hunan Research Center of Livestock&Poultry SciencesSouth-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of AgricultureInstitute of Subtropical AgricultureThe Chinese Academy of SciencesChangshaHunan 410125China Hunan International Joint Laboratory of Animal Intestinal Ecology and Health Laboratory of Animal Nutrition and Human HealthCollege of Life SciencesHunan Normal UniversityChangshaHunan410081China

Accumulating evidence implicates that offspring are susceptible to paternal alterations in numerous fetal disorders,such as growth and metabolic ***,less study has been conducted to define the relationship between paternal zinc deficiency(ZnD)and energy metabolism of *** the present study,we used a paternal ZnD exposure(Zn at 0.3 mg/g)model to test energy metabolism of male and female offspring with the intervention of diet type(high-fat diet and low-fat diet).Our results demonstrated that paternal ZnD decreased body weight(BW)gain per week(P<0.01)and ME intake per week(P<0.05)at 11 weeks in male offspring with high-fat diet intervention but not in female ***,anabolism and catabolism of hepatic energy products also exhibited *** attenuated liver glucose but increased lipids content accompanied with elevated adiponectin and reduction in leptin level in serum,which exhibited lipid metabolic disturbance and smaller ratio of liver weight to BW in male but not female *** qRT-PCR and liver energy metabolites analysis revealed that paternal ZnD mainly induced reduction in glucose tolerance and lowered glucose uptaking ability in male offspring and thereby alleviated glycolysis and the tricarboxylic acid cycle(TCA)cycle,which displayed a male ***,we propose that paternal ZnD abolishes metabolic effects in male offspring induced by diet type *** findings reveal a novel link between paternal Zn-D and offspring energy metabolism.

关键词： Zinc-deficiency Paternal Energy metabolism Offspring

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Role of iron in host-microbiota interaction and its effects on intestinal mucosal growth and immune plasticity in a piglet model

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Science China(Life Sciences) 2023年第9期66卷 2086-2098页

作者： Zhenglin Dong Shuan Liu Qingqing Deng Guanya Li yulong Tang Xin Wu Dan Wan yulong yin Key Laboratory of Agro-Ecological Processes in Subtropical Region Hunan Research Center of Livestock&Poultry SciencesSouth-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of AgricultureInstitute of Subtropical AgricultureChinese Academy of SciencesChangsha 410125China Hunan International Joint Laboratory of Animal Intestinal Ecology and Health Laboratory of Animal Nutrition and Human HealthCollege of Life SciencesHunan Normal UniversityChangsha 410081China

Iron is an essential trace element for both the host and resident microbes in the gut. In this study, iron was administered orally and parenterally to anemic piglets to investigate the role of iron in host-microbiota interaction and its effects on intestinal mucosal growth and immune plasticity. We found that oral iron administration easily increased the abundance of Proteobacteria and Escherichia-Shigella, and decreased the abundance of Lactobacillus in the ileum. Furthermore, similar bacterial changes, namely an increase in Proteobacteria, Escherichia-Shigella, and Fusobacterium and a reduction in the Christensenellaceae_R-7_group,were observed in the colon of both iron-supplemented groups. Spearman's correlation analysis indicated that the changed Fusobacterium, Fusobacteria and Proteobacteria in the colon were positively correlated with hemoglobin, colon and spleen iron levels. Nevertheless, it was found that activated m TOR1 signaling, improved villous height and crypt depth in the ileum,enhanced immune communication, and increased protein expression of IL-22 and IL-10 in the colon of both iron-supplemented groups. In conclusion, the benefits of improved host iron outweigh the risks of altered gut microbiota for intestinal mucosal growth and immune regulation in treating iron deficiency anemia.

关键词： iron deficiency iron-microbial interactions m TOR signaling immune regulation

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Low-protein diets supplemented with glutamic acid or aspartic acid ameliorate intestinal damage in weaned piglets challenged with hydrogen peroxide

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Animal Nutrition 2021年第2期7卷 356-364页

作者： Shuai Chen Xin Wu Jielin Duan Pan Huang Tiejun Li yulong yin Jie yin College of Animal Science and Technology Hunan Agriculture UniversityHunan Co-Innovation Center of Animal Production SafetyChangsha410128China Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central Ministry of AgricultureHunan Provincial Engineering Research Center of Healthy LivestockKey Laboratory of Agro-ecological Processes in Subtropical RegionInstitute of Subtropical AgricultureChinese Academy of SciencesChangsha410125China University of Chinese Academy of Sciences Beijing 100049China

Glutamic acid(Glu)and aspartic acid(Asp)are acidic amino acids with regulatory roles in nutrition,energy metabolism,and oxidative *** study aimed to evaluate the effects of low-protein diets supplemented with Glu and Asp on the intestinal barrier function and energy metabolism in weaned piglets challenged with hydrogen peroxide(H2O2).Forty piglets were randomly divided into 5 groups:NC,PC,PGA,PG,and PA(n=8 for each group).Pigs in the NC and PC groups were fed a low-protein diet,while pigs in the PGA,PG,or PA groups were fed the low-protein diet supplemented with 2.0%Glu+1.0%Asp,2.0%Glu,or 1.0%Asp,*** day 8 and 11,pigs in the NC group were intraperitoneally injected with saline(1 mL/kg BW),while pigs in the other groups were intraperitoneally administered 10%H2O2(1 mL/kg BW).On day 14,all pigs were sacrificed to collect jejunum and ileum following the blood sample collection in the ***,low-protein diets supplemented with Glu or Asp ameliorated the intestinal oxidative stress response in H2O2-challenged piglets by decreasing intestinal expression of genes(P<0.05)(e.g.,manganese superoxide dismutase[MnSOD],glutathione peroxidase[Gpx]-1,and Gpx-4)encoding oxidative stress-associated proteins,reducing the serum concentration of diamine oxidase(P<0.05),and inhibiting apoptosis of the intestinal *** and Asp supple-mentation attenuated the upregulated expression of energy metabolism-associated genes(such as hexokinase and carnitine palmitoyltransferase-1)and the H2O2-induced activation of acetyl-coenzyme A carboxylase(ACC)in the jejunum and adenosine monophosphate-activated protein kinase-acetyl-ACC signaling in the *** Glu and Asp also ameliorated intestinal barrier damage as indicated by restored intestinal histology and *** conclusion,low-protein diets supplemented with Glu and Asp protected against oxidative stress-induced intestinal dysfunction in piglets,suggesting that this approach could be used as a nut

关键词： Glutamate Aspartate Oxidative stress Hydrogen peroxide Apoptosis AMPK

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Dietary proline supplementation alters colonic luminal microbiota and bacterial metabolite composition between days 45 and 70 of pregnancy in Huanjiang mini-pigs

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Journal of Animal Science and Biotechnology 2018年第2期9卷 370-380页

作者： Yujiao Ji Qiuping Guo yulong yin Francois Blachier Xiangfeng Kong National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production Key Laboratory of Agro-ecological Processes in Subtropical RegionInstitute of Subtropical AgricultureChinese Academy of SciencesChangshaHunan 410125China Research Center of Mini-pig Huanjiang Observation and Research Station for Karst EcosystermsHuanjiangGuangxi 547100China UMR 914 INRA/AgroParisTech/Universite Paris-Sacaly Nutrition Physiology and Ingestive Behavior75005 ParisFrance

Background: Pregnancy is associated with important changes in gut microbiota composition. Dietary factors may affect the diversity, composition, and metabolic activity of the intestinal microbiota. Among amino acids, proline is known to play important roles in protein metabolism and structure, cell differentiation, conceptus growth and development, and gut microbiota re-equilibration in case of ***: Dietary supplementation with 1% proline decreased(P < 0.05) the amounts of Klebsiella pneumoniae,Peptostreptococcus productus, Pseudomonas, and Veillonella spp. in distal colonic contents than that in the control group. The colonic contents of Butyrivibrio fibrisolvens, Bifidobacterium sp., Clostridium coccoides, Clostridium coccoides-Eubacterium rectale, Clostridium leptum subgroup, Escherichia coli, Faecalibacterium prausnitzii,Fusobacterium prausnitzii, and Prevotella increased(P < 0.05) on d 70 of pregnancy as compared with those on d 45 of pregnancy. The colonic concentrations of acetate, total straight-chain fatty acid, and total short-chain fatty acids(SCFA) in the proline-supplemented group were lower(P < 0.05), and butyrate level(P = 0.06) decreased as compared with the control group. Almost all of the SCFA displayed higher(P < 0.05) concentrations in proximal colonic contents on d 70 of pregnancy than those on d 45 of pregnancy. The concentrations of 1,7-heptyl diamine(P = 0.09) and phenylethylamine(P < 0.05) in proximal colonic contents were higher, while those of spermidine(P = 0.05) and total bioamine(P = 0.06) tended to be lower in the proline-supplemented group than those in the control group. The concentrations of spermidine, spermine, and total bioamine in colonic contents were higher(P < 0.05) on d 70 of pregnancy than those measured on d 45 of pregnancy. In contrast, the concentration of phenylethylamine was lower(P < 0.05) on d 70 than on d 45 of pregnancy.(Continued on next page)(Continued from previous page)Conclusion: These findings i

关键词： Bacterial metabolites Colonic microbiota L-proline Pregnant Huanjiang mini-pigs

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Role of D-aspartate on biosynthesis,racemization,and potential functions:A mini-review

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Animal Nutrition 2018年第3期4卷 311-315页

作者： Yuying Li Hui Han Jie yin Tiejun Li yulong yin Key Laboratory of Agro-Ecological Processes in Subtropical Region National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry ProductionHunan Provincial Engineering Research Center for Healthy Livestock and Poultry ProductionScientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-CentralMinistry of AgricultureInstitute of Subtropical AgricultureChinese Academy of SciencesChangsha 410125China University of Chinese Academy of Sciences Beijing 100039China Hunan Co-Innovation Center of Animal Production Safety Changsha 410128China

D-aspartate, a natural and endogenous amino acid, widely exists in animal tissues and can be synthesized through aspartate racemase and transformed by D-aspartate oxidase(DDO). D-aspartate mainly serves as a neurotransmitter and has been demonstrated to exhibit various physiological functions,including nutritional potential, regulation on reproduction and hormone biology, and neuron *** article mainly reviews the synthesis, racemization, and physiological functions of D-aspartate with emphasis on the potential in diseases.

关键词： D-aspartate Biosynthesis Racemization Nutrition Functions

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