Low-protein diets supplemented with glutamic acid or aspartic acid ameliorate intestinal damage in weaned piglets challenged with hydrogen peroxide

作     者：Shuai Chen Xin Wu Jielin Duan Pan Huang Tiejun Li Yulong Yin Jie Yin Shuai Chen;Xin Wu;Jielin Duan;Pan Huang;Tiejun Li;Yulong Yin;Jie Yin

作者机构：College of Animal Science and TechnologyHunan Agriculture UniversityHunan Co-Innovation Center of Animal Production SafetyChangsha410128China Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-CentralMinistry of AgricultureHunan Provincial Engineering Research Center of Healthy LivestockKey Laboratory of Agro-ecological Processes in Subtropical RegionInstitute of Subtropical AgricultureChinese Academy of SciencesChangsha410125China University of Chinese Academy of SciencesBeijing 100049China 

出 版 物：《Animal Nutrition》 (动物营养（英文版）)

年 卷 期：2021年第7卷第2期

页      面：356-364页

核心收录：

学科分类：0710[理学-生物学] 090502[农学-动物营养与饲料科学] 0905[农学-畜牧学] 0906[农学-兽医学] 09[农学] 

基　　金：the National Natural Science Foundation of China(NO.31272463) the Hunan Provincial Natural Science Foundation of China(NO.12JJ2014) the Changsha Science and Technology Key Program(K1307007-21) the Chinese Academy of Science STS Project(KFJ-EW-STS-063) 

主　　题：Glutamate Aspartate Oxidative stress Hydrogen peroxide Apoptosis AMPK 

摘      要：Glutamic acid(Glu)and aspartic acid(Asp)are acidic amino acids with regulatory roles in nutrition,energy metabolism,and oxidative *** study aimed to evaluate the effects of low-protein diets supplemented with Glu and Asp on the intestinal barrier function and energy metabolism in weaned piglets challenged with hydrogen peroxide(H2O2).Forty piglets were randomly divided into 5 groups:NC,PC,PGA,PG,and PA(n=8 for each group).Pigs in the NC and PC groups were fed a low-protein diet,while pigs in the PGA,PG,or PA groups were fed the low-protein diet supplemented with 2.0%Glu+1.0%Asp,2.0%Glu,or 1.0%Asp,*** day 8 and 11,pigs in the NC group were intraperitoneally injected with saline(1 mL/kg BW),while pigs in the other groups were intraperitoneally administered 10%H2O2(1 mL/kg BW).On day 14,all pigs were sacrificed to collect jejunum and ileum following the blood sample collection in the ***,low-protein diets supplemented with Glu or Asp ameliorated the intestinal oxidative stress response in H2O2-challenged piglets by decreasing intestinal expression of genes(P0.05)(e.g.,manganese superoxide dismutase[MnSOD],glutathione peroxidase[Gpx]-1,and Gpx-4)encoding oxidative stress-associated proteins,reducing the serum concentration of diamine oxidase(P0.05),and inhibiting apoptosis of the intestinal *** and Asp supple-mentation attenuated the upregulated expression of energy metabolism-associated genes(such as hexokinase and carnitine palmitoyltransferase-1)and the H2O2-induced activation of acetyl-coenzyme A carboxylase(ACC)in the jejunum and adenosine monophosphate-activated protein kinase-acetyl-ACC signaling in the *** Glu and Asp also ameliorated intestinal barrier damage as indicated by restored intestinal histology and *** conclusion,low-protein diets supplemented with Glu and Asp protected against oxidative stress-induced intestinal dysfunction in piglets,suggesting that this approach could be used as a nut