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胃癌高低发区差异基因型幽门螺杆菌菌株对人胃上皮细胞系 GES-1的损伤作用

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世界华人消化杂志 2005年第22期13卷 2681-2684页

作者：何红梅宫月华袁媛中国医科大学附属第一医院肿瘤研究所第三研究室辽宁省沈阳市110001

目的:研究胃癌高、低发区差异基因型幽门螺杆(H pylori) 菌株对永生化的非肿瘤胃上皮细胞GES-1的损伤作用;并阐明H pylori致细胞损伤的分子机制．方法:通过常规HE染色、Gimsa染色、W-S银染色鉴定H pylori菌株,采用蛋白酶K及酚-氯仿抽提... 详细信息

目的:研究胃癌高、低发区差异基因型幽门螺杆(H pylori) 菌株对永生化的非肿瘤胃上皮细胞GES-1的损伤作用;并阐明H pylori致细胞损伤的分子机制．方法:通过常规HE染色、Gimsa染色、W-S银染色鉴定H pylori菌株,采用蛋白酶K及酚-氯仿抽提法提取DNA,PCR法扩增cagA、vacAsl、s2、mla、mlb、m2基因,对H pylori 因亚型进行检测．利用细胞和幽门螺杆菌共培养技术,观察胃癌高低发区差异基因亚型H pylori以及其他两种非差异基因型H pylori对胃上皮细胞系GES-1的损伤作用,并通过免疫细胞化学方法检测GES-1细胞8-OHdG的表达．结果:幽门螺杆菌对GES-1细胞具有损伤作用,随着作用时间的延长,GES-1细胞形态由贴壁的梭形变为圆形．细胞核染色质浓缩,呈新月形聚集于核缘,细胞质浓缩,可见空泡变性,死亡细胞逐渐增多．差异基因型H pylori对GES-1细胞的损伤程度比非差异基因型的损伤作用重．正常对照组 8-OHdG表达基本阴性(0．5%),而H pylori处理组GES-1细胞 8-OHdG表达阳性达98．5％,二者差异显著(P<0．01),有统计学意义．结论:胃癌高发区差异基因型H pylori即cagA+,vacAs1+／m1b+ 对胃上皮细胞GES-1具有更强损伤作用,其损伤具有时间依赖关系;H pylori可能通过增加胃上皮细胞8-OhdG表达而导致正常胃黏膜上皮细胞恶性转化．

关键词：胃癌幽门螺杆菌差异基因型胃黏膜上皮细胞共培养 8-OHdG

PGC基因插入/缺失多态对胃粘膜及血清PGC蛋白表达的影响

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癌症 2009年第5期28卷 487-492页

作者：孙丽萍宫月华董楠楠王兰袁媛中国医科大学第一附属医院肿瘤研究所辽宁沈阳110001

背景与目的:胃蛋白酶原C(pepsinogen C,PGC)是胃粘膜特异性功能酶的前体,PGC基因插入/缺失多态与胃癌易感性相关。本研究旨在探讨PGC基因多态与PGC蛋白表达的关系。方法:常规酚-氯仿法提取浅表性胃炎(superficial gastritis,SG)、胃糜... 详细信息

背景与目的:胃蛋白酶原C(pepsinogen C,PGC)是胃粘膜特异性功能酶的前体,PGC基因插入/缺失多态与胃癌易感性相关。本研究旨在探讨PGC基因多态与PGC蛋白表达的关系。方法:常规酚-氯仿法提取浅表性胃炎(superficial gastritis,SG)、胃糜烂溃疡(gastric ulcer,GEU)、萎缩性胃炎(atrophic gastritis,AG)及胃癌(gastric cancer,GC)患者共493例血凝块基因组DNA。PCR法扩增PGC基因7～8外显子间的100bp插入/缺失片段,检测PGC基因多态型,多态片段用测序证实。免疫组织化学方法检测胃粘膜PGC蛋白表达水平。ELISA法检测血清PGC蛋白含量。结果:从SG→GEU→GA→GC:PGC等位基因1纯合型分布频率逐渐升高,GC组显著高于SG组(P=0.018);胃粘膜PGC蛋白阳性率依次显著降低(均P<0.01),强阳性率逐渐降低,除SG组与GEU组外,其余各组间差异均有统计学意义(P<0.05);血清PGC蛋白含量GEU组和GC组显著高于SG组(P=0.000,P=0.000)。PGC基因1纯合型与胃粘膜PGC蛋白表达强度呈负相关(r=-0.1085,P=0.023),与PGC血清含量无相关性(P=0.435)。从等位基因1纯合型→等位基因1杂合型→其它型:PGC阳性率逐渐升高,等位基因1纯合型组与其它型之间的差异有统计学意义(P=0.009);SG组PGC等位基因1纯合型的PGC强阳性率低于其它型组(P=0.047)。结论:PGC基因多态与胃粘膜PGC蛋白表达呈负相关,与血清PGC含量无相关性。

关键词：胃蛋白酶原C 基因多态胃肿瘤

幽门螺杆菌在胃癌及其癌前疾病中的原位检测及意义

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世界华人消化杂志 2008年第33期16卷 3740-3745页

作者：于秀文董楠楠宫月华徐倩袁媛中国医科大学附属第一医院普通外科研究所暨肿瘤研究所第三研究室辽宁省高校肿瘤病因与预防重点实验室辽宁省沈阳市110001

目的:探讨H pylori感染在胃癌及癌前疾病中的分布状态以及在胃癌的发生及分化中的作用以及免疫组化法检测H pylori感染的价值.方法:随机选取辽宁省庄河地区不同胃疾病胃黏膜活检标本327例,包括慢性浅表性胃炎63例、萎缩性胃炎62例,胃癌... 详细信息

目的:探讨H pylori感染在胃癌及癌前疾病中的分布状态以及在胃癌的发生及分化中的作用以及免疫组化法检测H pylori感染的价值.方法:随机选取辽宁省庄河地区不同胃疾病胃黏膜活检标本327例,包括慢性浅表性胃炎63例、萎缩性胃炎62例,胃癌202例,常规HE染色及免疫组织化学染色,观察黏膜、黏液H pylori感染密度.结果:不同胃疾病H pylori在胃黏膜定植状态为浅表性胃炎、萎缩性胃炎高于胃癌(χ2=96.998,87.542,均P2时,浅表性胃炎、萎缩性胃炎高于胃癌(χ2=20.252,46.248,均P<0.01),萎缩性胃炎高于浅表性胃炎(χ2=4.746,P<0.05),胃癌与癌周黏膜无统计学差异,肠型胃癌高于弥漫型胃癌(χ2=6.784,P<0.01).结论:不同胃疾病H pylori分布,浅表性胃炎、萎缩性胃炎高于胃癌,胃癌低于癌周黏膜,肠型高于弥漫型.黏液内游离H pylori密度评分胃癌组织与癌周黏膜无差异.免疫组化染色检测H pylori优于HE.

关键词：幽门螺杆菌免疫组织化学原位检测胃疾病分布状态

中国辽宁地区人群幽门螺杆菌感染菌株与相关性胃疾病的关系

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世界华人消化杂志 2007年第33期15卷 3462-3467页

作者：宫月华柳云恩孙丽萍董楠楠袁媛中国医科大学附属第一医院肿瘤研究所第三研究室辽宁省沈阳市110001

目的:探讨中国辽宁地区人群H pylori致病基因的疾病相关性,为揭示H pylori的致病机制及监测H pylori相关性胃疾病的高危人群提供线索.方法:选取胃黏膜活检标本491例,分组浅表性胃炎(GS)、萎缩性胃炎(GA)、溃疡(GU)、胃癌(GC),在微需氧... 详细信息

目的:探讨中国辽宁地区人群H pylori致病基因的疾病相关性,为揭示H pylori的致病机制及监测H pylori相关性胃疾病的高危人群提供线索.方法:选取胃黏膜活检标本491例,分组浅表性胃炎(GS)、萎缩性胃炎(GA)、溃疡(GU)、胃癌(GC),在微需氧的条件下,培养出H pylori 222例,并用标准的酚-氯仿方法提取菌种DNA后经聚合酶链反应及琼脂糖凝胶电泳对cagA,vacA,iceA基因亚型进行检测.同时取胃窦、体、角黏膜各1块,经石蜡切片,HE染色,行组织病理学诊断.结果:GA组感染m2(43.1%)亚型菌株构成比最高,与GU(18.2%)和GC(17.9%)组相比差异有统计学意义(P=0.015,P=0.020),与GS(30.00%)组相比差异没有统计学意义(P= 0.084).在GA组中,感染s1m2亚型菌株有22例(44.9%),与本组病例中其他基因亚型相比差别均有统计学意义,与Gu、GC组感染s1m2亚型菌株相比差别有统计学意义(P=0.039),与GS组相比差别没有统计学意义(P=0.067).结论:中国辽宁地区人群感染s1m2型菌株与萎缩性胃炎发生有关.

关键词：幽门螺杆菌相关性胃疾病萎缩性胃炎聚合酶链反应

Infiltration by monocytes of the central nervous system and its role in multiple sclerosis: reflections on therapeutic strategies

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Neural Regeneration Research 2025年第3期20卷 779-793页

作者： Guangyong Zhang Qing Yao Chubing Long Pengcheng Yi Jiali Song Luojia Wu Wei Wan Xiuqin Rao yue Lin Gen Wei Jun Ying Fuzhou hua Department of Anesthesiology the Second Affiliated Hospital of Nanchang UniversityNanchangJiangxi ProvinceChina Key Laboratory of Anesthesiology of Jiangxi Province NanchangJiangxi ProvinceChina

Mononuclear macrophage infiltration in the central nervous system is a prominent feature of neuroinflammation. Recent studies on the pathogenesis and progression of multiple sclerosis have highlighted the multiple roles of mononuclear macrophages in the neuroinflammatory process. Monocytes play a significant role in neuroinflammation, and managing neuroinflammation by manipulating peripheral monocytes stands out as an effective strategy for the treatment of multiple sclerosis, leading to improved patient outcomes. This review outlines the steps involved in the entry of myeloid monocytes into the central nervous system that are targets for effective intervention: the activation of bone marrow hematopoiesis, migration of monocytes in the blood, and penetration of the blood–brain barrier by monocytes. Finally, we summarize the different monocyte subpopulations and their effects on the central nervous system based on phenotypic differences. As activated microglia resemble monocyte-derived macrophages, it is important to accurately identify the role of monocyte-derived macrophages in disease. Depending on the roles played by monocyte-derived macrophages at different stages of the disease, several of these processes can be interrupted to limit neuroinflammation and improve patient prognosis. Here, we discuss possible strategies to target monocytes in neurological diseases, focusing on three key aspects of monocyte infiltration into the central nervous system, to provide new ideas for the treatment of neurodegenerative diseases.

关键词： blood–brain barrier macrophages monocytes multiple sclerosis neuroinflammation review therapy

幽门螺杆菌致胃上皮细胞株GES-1和胃癌细胞株SGC-7901的氧化性损伤

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世界华人消化杂志 2009年第35期17卷 3590-3594页

作者：王艳丽宫月华徐莹陈铁军柳云恩袁媛中国医科大学附属第一医院肿瘤研究所暨普通外科研究所第三研究室辽宁省高校肿瘤病因与预防重点实验室辽宁省沈阳市110001 辽宁医学院附属第一医院肿瘤科辽宁省锦州市121001

目的:探讨Hpylori对人正常胃黏膜上皮细胞永生细胞株GES-1和人淋巴结转移胃腺癌细胞株SGC-7901的氧化性DNA损伤作用.方法:采用细菌-细胞共培养的方法,比较Hpylori作用前后GES-1和SGC-7901细胞株的形态学变化;采用激光扫描共聚焦显微镜方... 详细信息

目的:探讨Hpylori对人正常胃黏膜上皮细胞永生细胞株GES-1和人淋巴结转移胃腺癌细胞株SGC-7901的氧化性DNA损伤作用.方法:采用细菌-细胞共培养的方法,比较Hpylori作用前后GES-1和SGC-7901细胞株的形态学变化;采用激光扫描共聚焦显微镜方法,比较Hpylori作用前后GES-1和SGC-7901细胞株8-羟基脱氧鸟苷(8-OhdG)的表达.结果:Hpylori对GES-1和SGC-7901细胞株均具有损伤作用;8-OHdG表达升高,加菌组与对照组相比差别具有统计学意义(64.9396±17.8142 vs 32.3010±7.3620;102.8344±30.2632 vs 77.1336±32.3223,均P=0.000);而且8-OHdG表达的变化程度GES-1细胞显著高于SGC-7901细胞.结论:Hpylori能够诱导GES-1和SGC-7901细胞DNA氧化性损伤显著增加;在Hpylori氧化损伤的相关研究中,更适宜选择对损伤作用敏感的GES-1细胞株作为研究对象.

关键词：幽门螺杆菌 GES-1 SGC-7901 氧化性DNA损伤 8-OHdG 共培养

Grain Sink Strength Related to Carbon Staying in the Leaves of Hybrid Wheat XN901

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Agricultural Sciences in China 2009年第5期8卷 546-555页

作者： gong yue-hua JI Xiao-hui GAO Jun-feng College of Life Science Northwest A&F University Yangling 712100 P.R.China School of Chemistry and Environment Science Shaanxi University of Technology Hanzhong 723001 P.R.China

XN901 is a K-type three-line hybrid wheat with a high yield potential, and its leaves and stem remaining green during grain maturation, suggesting much assimilate stay in leaves and stem. The grain water content, grain volume, carbohydrate content, and enzyme activity of sucose metabolism in the grain, as well as source-sink relationship were studied in order to investigate the physiological reason of the assimilate remaining in leaves and stem at the late stage. The results showed that the hybrid grains had more water and soluble sugar, higher activities of acid invertase and sucrose synthase at the early stage that led to a faster expansion growth, greater grain volume and faster starch synthesis at the early to mid stage of grain development. Also it had a longer period for actively filling. As a result, the grain weight and yield of the hybrid were increased by 14 and 15% respectively compared to that of Shaan 229. Additionally, the biomass of XN901 was 41.7% more than that of control, but its harvest index was 9% lower than Shaan 229. However, its lower activity of sucrose synthase indicated a lower sink activity at the late stage, resulting in a slow rate of filling and starch synthesis. Also, the hybrid wheat XN901 had a large source-sink ratio. It is the main reason for much assimilate remaining in the straw at the late stage and lower harvest index. Strengthening the sink activity and raising the harvest index should be the key means of improving the yield of hybrid wheat.

关键词： hybrid wheat stay-green sink strength sink activity sink size

Forward Modeling of Azimuthal Anisotropy to the Reflected P Wave of Coal Seam

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Journal of China University of Mining and Technology 2006年第3期16卷 321-324页

作者： ZHANG Jun-gong DONG Shou-hua yue Jian-hua School of Mechanical and Civil Engineering China University of Mining ＆ Technology Beifing 100083 China School of Resource and Earth Sciences China University of Mining ＆ Technology Xuzhou Jiangsu 221008 China

Under the condition of weak anisotropy, the relation of P-wave anisotropy in direction to fractures of coal seams was researched in order to forecast the density and the direction of the fractures. Although the approximate solution by Rtiger is suitable for thick reservoirs, it has some limitations for the composite reflected wave from both roofs and floors of coal seams, as well as multiple reflections. So first, the phase velocity and group velocity as well as their travel time were calculated about the reflected P-wave of the coal seam. Then, the anisotropic coefficients of both roofs and floors were calculated by Rueger formulae and last, the section versus azimuth in fixed offset can be gotten by convolution. In addition, the relation of amplitude of the composite reflected wave to azimuth angle was discussed. The forward modelling results of the coal azimuth anisotropy show these： 1） the coal seam is the strong reflecting layer, but the change of the reflectivity caused by the azimuth anisotropy is smaller; 2） if the azimuth angle is parallel to the crack strike, the reflectivity reaches up to the maximum absolute value, however, if the azimuth angle is perpendicular to the crack strike, the absolute value of the reflection coefficient is minimum; and 3）the reflection coefficient is the cosine function of the azimuth angle and the period is π.

关键词： coal seam composite wave anisotropy P wave

Desulfurization using the 1,2-dimethylimidazolium ionic liquid as an adsorbent

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Chinese Chemical Letters 2015年第3期26卷 317-319页

作者： yue-Qin Cai gong-hua Song Dian-Ni Liang School of Chemistry and Molecular Engineering East China University of Science and Technology Shanghai Key Laboratory of Chemical Biology Institute of Pesticides & PharmaceuticalsEast China University of Science and Technology

The aim of this study is to investigate the possible use of a 1,2-dimethylimidazolium ionic liquid,2,2-bis（（1,2-dimethylimidazolium）methyl）propane-1,3-diol hexafluorophosphate（1）,as an adsorbent to selectively remove aromatic heterocyclic sulfur compounds from model *** result indicates that adsorbent 1 is insoluble in model *** spent IL saturated sulfur compounds could be regenerated by a water dilution *** influence of extraction time or temperature as well as the molar ratio of 1 to aromatic heterocyclic sulfur compound was also studied.

关键词： adsorbent sulfur remove aromatic heterocyclic ionic insoluble regenerated saturated molar