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Curcumin Inhibits Proliferation of Renal Cell Carcinoma in vitro and in vivo by Regulating miR-148/ADAMTS18 through Suppressing Autophagy

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Chinese Journal of Integrative Medicine 2023年第8期29卷 699-706页

作者： XU Ben yuan chang-wei ZHANG Jia-en Depatment of Urology Peking University First HospitalBeijing(100034)China

Objective: To explore the effect of curcumin on the proliferation of renal cell carcinoma and analyze its regulation mechanism. Methods: In RCC cell lines of A498 and 786-O, the effects of curcumin(2.5, 5, 10 μmo/L) on the proliferation were analyzed by Annexin V+PI staining. Besides, A498 was inoculated into nude mice to establish tumorigenic models, and the model mice were treated with different concentrations of curcumin(100, 200, and 400 mg/kg), once daily for 30 days. Then the tumor diameter was measured, the tumor cells were observed by hematoxylin-eosin staining, and the protein expressions of miR-148 and ADAMTS18were detected by immunohistochemistry. In vitro, after transfection of miR-148 mimics, miR-148 inhibitor or si-ADAMTS18 in cell lines, the expression of ADAMTS18 was examined by Western blotting and the cell survival rate was analyzed using MTT. Subsequently, Western blot analysis was again used to examine the autophagy phenomenon by measuring the relative expression level of LC3-Ⅱ/LC3-Ⅰ;autophagy-associated genes,including those of Beclin-1 and ATG5, were also examined when miR-148 was silenced in both cell lines with curcumin treatment. Results: Curcumin could inhibit the proliferation of RCC in cell lines and nude mice. The expressions of miR-148 and ADAMTS18 were upregulated after curcumin treatment both in vitro and in vivo(P<0.05). The cell survival rate was dramatically declined upon miR-148 or ADAMTS18 upregulated. However,si-ADAMTS18 treatment or miR-148 inhibitor reversed these results, that is, both of them promoted the cell survival rate. Conclusion: Curcumin can inhibit the proliferation of renal cell carcinoma by regulating the miR-148/ADAMTS18 axis through the suppression of autophagy in vitro and in vivo. There may exist a positive feedback loop between mi R-148 and ADAMTS18 gene in RCC.

关键词： curcumin renal cell carcinoma MiR-148 ADAMTS18 gene autophagy

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Curcumin Interferes with TGF-β1-Induced Fibrosis in NRK-49F Cells by Reversing ADAMTS18 Gene Methylation

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Chinese Journal of Integrative Medicine 2024年第7期30卷 600-607页

作者： XU Ben ZHANG Jia-en YE Lin yuan chang-wei Department of Urology Peking University First Hospital and Institute of UrologyPeking UniversityNational Urological Cancer CenterBeijing100034China

Objective To explore the molecular mechanism by which curcumin affects renal interstitial fibrosis(RIF)progression by regulating ADAM metallopeptidase with thrombospondin type 1 motif 18(ADAMTS18)*** NRK-49F cells RIF model were induced with transforming growth factorβ1(TGF-β1).Effects of different concentrations of curcumin(0,10,20,and 30μmol/L)on cell proliferation,cell cycle,cell apoptosis as well as cyclin D1 expression were analyzed by cell counting kit-8,flow cytometry and Western blot,***18 methylation levels were determined by methylation-specific polymerase chain ***18,fibronectin(FN),type I collagen(Col-I)and alpha-smooth muscle actin(α-SMA)mRNA and protein expressions were analyzed by real-time PCR(RT-PCR)and Western blot,***,cells were treated with 50 mmol/L 5-aza-2′-deoxycytidine(5-aza-dC,demethylation agent)for 72 *** of curcumin on extracellular matrix(ECM)deposition was evaluated by immunochemical staining and Western ***-49F cells were transfected with ADAMTS18 small interfering RNA and grouped into a normal control,ADAMTS18-knock-out(KO),and ADAMTS18-KO+30μmol/L curcumin groups,and whether curcumin can reverse the effect of ADAMTS18 knockdown on RIF was *** Compared with the control group,TGF-β1 significantly inhibited the proliferation of NRK-49F cells,blocked the G1/G0 phase,promoted cell apoptosis and inhibited cyclin D1 expression(P<0.01).Among the different concentrations of curcumin,30μmol/L curcumin significantly reversed these processes(P<0.01).Immunochemical staining and Western blot results showed that curcumin significantly inhibited the deposition of FN,Col-I andα-SMA(P<0.01).Curcumin and 5-zaz-dC had synergistic effects,promoting ADAMTS18 expression,removing ADAMTS18 methylation,and reducing ECM ***18 knockdown promoted ECM accumulation,and curcumin reversed this process(P<0.01).Conclusion TGF-β1-induced fibrosis in NRK-49F ***

关键词： renal fibrosis curcumin Chinese medicine ADAMTS18 methylation modification

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公路网规划研究进展与发展趋势

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中国公路学报 2007年第3期20卷 91-96页

作者：吴群琪袁长伟长安大学经济与管理学院陕西西安710064

为了科学、有效地促进中国公路网的建设与规划,推动公路网规划理论方法的研究,系统地回顾了各国公路网规划理论方法的发展历程与实践应用现状,介绍了现有公路网规划理论、方法体系,并对这些理论体系进行了比较评述。在结合中国公路网规... 详细信息

为了科学、有效地促进中国公路网的建设与规划,推动公路网规划理论方法的研究,系统地回顾了各国公路网规划理论方法的发展历程与实践应用现状,介绍了现有公路网规划理论、方法体系,并对这些理论体系进行了比较评述。在结合中国公路网规划实践的基础上,认为目前公路网规划在整体规划依据与各方法体系的细节上存在缺陷,指出现代科学技术的应用、定量规划方法和多学科的融合将是未来发展的趋势。

关键词：交通工程公路网规划评论研究进展发展趋势

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中国交通运输经济发展与碳排放效率评价

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中国公路学报 2019年第1期32卷 154-161页

作者：陈思茹张帅袁长伟陕西交通职业技术学院经济管理学院陕西西安710018 南开大学经济学院天津300071 长安大学经济与管理学院陕西西安710064

为评价交通运输经济与碳排放绩效,探寻经济和碳减排双赢的改进路径,提出交通运输经济效率、碳排放效率及联合效率概念。针对中国30个省市区,以资本存量、煤类消费量、油类消费量、新能源消费量、从业人员作为投入要素,交通运输综合换算... 详细信息

为评价交通运输经济与碳排放绩效,探寻经济和碳减排双赢的改进路径,提出交通运输经济效率、碳排放效率及联合效率概念。针对中国30个省市区,以资本存量、煤类消费量、油类消费量、新能源消费量、从业人员作为投入要素,交通运输综合换算周转量和行业增加值作为期望产出,CO_2排放量作为非期望产出,采用RAM模型测度评估中国30个省市区的交通运输行业经济、碳排放及联合效率,探究3种效率的分布特征及变动规律,并利用非效率分解模型研究非效率来源,探索改进方向。研究结果表明:各省市区之间的交通运输经济效率、碳排放效率和联合效率均存在明显差异,且东部、中部、西部和东北地区呈现不同的梯度分布特性; 2006～2016年的经济效率、碳排放效率及联合效率总体上可以分为2个阶段,第1阶段为2006～2011年,经济效率、碳排放效率和联合效率均呈波动上升,第2阶段为2011～2016年,经济效率、碳排放效率和联合效率轻微波动,并逐渐趋于平稳。非效率分解模型表明:能源过度消耗是经济效率、碳排放效率和联合效率的主要非效率来源; CO_2过度排放是碳排放效率和联合效率非效率的主要来源;行业增加值产出不足是经济效率和联合效率非效率的主要来源。这说明推广新能源,提升交通运输运营效率,革新交通减排技术是能够实现双赢的重要路径。

关键词：交通工程碳减排 RAM模型双赢绩效非效率来源

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Melting Evaluation of Phase change Materials Impregnated into Cascaded Metal Foams with Regionalized Enhancement

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Journal of Thermal Science 2025年第1期34卷 223-241页

作者： wei Xinyi ZHANG Nan ZHANG Zhaoli CAO Xiaoling yuan Yanping School of Mechanical Engineering Southwest Jiaotong University

Metal foam promotes the heat transfer of phase change materials(PCMs) in the penalty of reducing the energy storage density of the composite *** this work,the effects of constant porosity(0.96,0.94,0.92,or 0.90) and pore density(PPI) of metal foam on heat transfer of composite PCMs are *** rate could be enhanced by employing with low porosity copper ***,aiming to the right bottom phase changing "dead region",a regionalized enhancement strategy of cascaded metal foams is *** dynamic melting performances of all the composite PCMs are comprehensively *** results reveal that the cascaded configuration is beneficial for *** show that the horizontal strategy enhances melting performance:a maximum of 17.98% reduction in total melting time could be reached when the rear part porosity is 90%.The energy storage density rate could be raised by 5.48%.Besides,the vertical strategy performs with a better average temperature uniformity of 0.441 and brings a lower temperature in the heated *** sum up,the regionalized enhancement of copper foam provides better performance in the phase change *** shows significant potential for solar heat storage and thermal protection.

关键词： copper metal foam heat transfer enhancement phase change materials regionalized enhancement

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运输经济学在国际上的若干研究重点及启示

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交通运输系统工程与信息 2013年第6期13卷 11-17页

作者：袁长伟吴群琪长安大学经济与管理学院西安710064

运输经济学关注于政策目标、需求、补贴、管制等运输经济重点问题,但国内运输经济学研究在很长一段时间里难以与主流经济学完美融合,在实践中缺乏对现实重大问题的分析与解释.本文回顾了近期国际上运输经济学的若干研究重点领域及进展,... 详细信息

运输经济学关注于政策目标、需求、补贴、管制等运输经济重点问题,但国内运输经济学研究在很长一段时间里难以与主流经济学完美融合,在实践中缺乏对现实重大问题的分析与解释.本文回顾了近期国际上运输经济学的若干研究重点领域及进展,在回顾学科演进的基础上,追溯运输经济学中政策目标、规模与成本、管制改革等核心问题研究的进展与展望.结合国内交通运输发展的阶段性特点,提炼当前运输经济学中的速度与风险、空间因素、综合协调等研究重点,为国内的运输经济学的研究、实践提供相关借鉴.

关键词：交通运输经济研究重点展望研究启示

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Tranylcypromine upregulates Sestrin 2 expression to ameliorate NLRP3-related noise-induced hearing loss

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Neural Regeneration Research 2025年第5期20卷 1483-1494页

作者： Xihang Chen Zhifeng Chen Menghua Li weiwei Guo Shuolong yuan Liangwei Xu chang Lin Xi Shi wei Chen Shiming Yang Senior Department of Otolaryngology Head and Neck Surgery Chinese PLA General HospitalChinese PLA Medical SchoolBeijingChina State Key Laboratory of Hearing and Balance Science BeijingChina National Clinical Research Center for Otolaryngologic Diseases BeijingChina Key Laboratory of Hearing Science Ministry of EducationBeijingChina Beijing Key Laboratory of Hearing Impairment Prevention and Treatment BeijingChina Department of Otorhinolaryngology Head and Neck Surgery The First Affiliated Hospital of Fujian Medical UniversityFuzhouFujian ProvinceChina Department of Otolaryngology Head and Neck Surgery The 940th Hospital of Joint Logistics Support Force of Chinese PLALanzhouGansu ProvinceChina Key Laboratory of Tropical Biological Resources of Ministry of Education School of Pharmaceutical SciencesHainan UniversityHaikouHainan ProvinceChina Academician Workstation of Hainan University School of Pharmaceutical SciencesYazhou BaySanyaHainan ProvinceChina

Noise-induced hearing loss is the primary non-genetic factor contributing to auditory ***,there are currently no effective pharmacological interventions for patients with noise-induced hearing ***,we present evidence suggesting that the lysine-specific demethylase 1 inhibitor–tranylcypromine is an otoprotective agent that could be used to treat noise-induced hearing loss,and elucidate its underlying regulatory *** established a mouse model of permanent threshold shift hearing loss by exposing the mice to white broadband noise at a sound pressure level of 120 d B for 4 *** found that tranylcypromine treatment led to the upregulation of Sestrin2(SESN2)and activation of the autophagy markers light chain 3B and lysosome-associated membrane glycoprotein 1 in the cochleae of mice treated with *** noise exposure group treated with tranylcypromine showed significantly lower average auditory brainstem response hearing thresholds at click,4,8,and 16 k Hz frequencies compared with the noise exposure group treated with *** findings indicate that tranylcypromine treatment resulted in increased SESN2,light chain 3B,and lysosome-associated membrane glycoprotein 1 expression after noise exposure,leading to a reduction in levels of 4-hydroxynonenal and cleaved caspase-3,thereby reducing noise-induced hair cell ***,immunoblot analysis demonstrated that treatment with tranylcypromine upregulated SESN2 expression via the autophagy *** treatment also reduced the production of NOD-like receptor family pyrin domaincontaining 3(NLRP3)*** conclusion,our results showed that tranylcypromine treatment ameliorated cochlear inflammation by promoting the expression of SESN2,which induced autophagy,thereby restricting NLRP3-related inflammasome signaling,alleviating cochlear hair cell loss,and protecting hearing *** findings suggest that inhibiting lysine-specific demethylase 1 is a

关键词： 4-hydroxynonenal apoptosis autophagy cleaved caspase-3 inflammation NOD-like receptor family pyrin domain-containing 3(NLRP3) noise-induced hearing loss oxidative stress Sestrin2 tranylcypromine

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双核都市圈运输通道旅客分担率模型及应用

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长安大学学报（自然科学版） 2007年第6期27卷 80-83页

作者：袁长伟吴群琪长安大学经济与管理学院陕西西安710064

为预测双核都市圈运输通道内旅客运输分担率,结合双核都市圈运输通道的旅客运输特性,通过将不同特性的变量引入效用函数,对Logit模型的应用加以改进,以成都—重庆运输通道为例,进行了旅客运输方式分担与线路分配的联合应用。结果表明:... 详细信息

为预测双核都市圈运输通道内旅客运输分担率,结合双核都市圈运输通道的旅客运输特性,通过将不同特性的变量引入效用函数,对Logit模型的应用加以改进,以成都—重庆运输通道为例,进行了旅客运输方式分担与线路分配的联合应用。结果表明:改进后的模型能有效地进行双核都市圈运输通道旅客运输方式分担与线路分配的预测;成渝(成都—重庆)运输通道中,成渝(成都—重庆)高速公路、成遂渝(成都—遂宁—重庆)高速公路、成遂渝(成都—遂宁—重庆)准高速铁路的分担率约为29%、33%、38%。

关键词：交通工程旅客分担率 Logit模型运输通道

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Resistance of Cement-based Grouting Materials with Nano- SiO_(2) Emulsion to Chloride Ion Penetration

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Journal of Wuhan University of Technology(Materials Science) 2025年第1期40卷 114-119页

作者： LI Shuiping CHENG Jian wei Chao yuan Bin YU Chengxiao College of Architectural Science and Engineering Yangzhou UniversityYangzhou 225127China

The chloride penetration resistance of cement-based grout materials was improved by nano-silica *** of mixtures containing different nano-silica particles or emulsions were exposed in sodium chloride solutions of specific concentrations with different test *** properties of the mixes were assessed in terms of weight loss and compressive strength.X-ray diffraction(XRD)and scanning electron microscopy(SEM)of mixes were performed to analysis the phase evolution and *** results demonstrated that the introduction of nano-SiO_(2) emulsion significantly decreased the compressive strength loss and calcium hydroxide(CH)crystal content of hydration production,and then enhanced the resistance of cement-based grouting materials to chloride ion *** improvement derives from the filling and pozzolanic effects of nano-SiO_(2) particles,which were incorporated via an emulsion and attributed to a well dispersion in grouting matrix.

关键词： grouting materials nano-SiO_(2)emulsion chloride ion penetration,weight loss strength loss

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Dry wear behavior of rheo-casting Al-16Si-4Cu-0.5Mg alloy

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Transactions of Nonferrous Metals Society of China 2016年第11期26卷 2818-2829页

作者：胡钊华吴国华徐佳莫文飞李艳磊刘文才张亮丁文江 Jonathan QUAN yuan-wei chang 上海交通大学轻合金精密成型国家工程研究中心与金属基复合材料国家重点实验室上海200240 Department of Materials Science and Engineering University of California Los Angeles

Dry wear behavior of the rheo-casting Al-16Si-4Cu-0.5Mg alloy was investigated by micro-scratch and dry sliding weartests. Analyses of the microstructure, scratch grooves, wear tracks, worn surfaces and wear debris of the alloy were carried out byoptical microscope and scanning electron microscope. The microstructural analysis showed that via rheo-processing, the primary Siwas refined and rounded, eutectics dispersed more homogenously, and even the skeleton AlFeMnSi phase was fragmented into facetshape. Micro-scratch test showed that the microstructural refinement resulted in better wear resistance. Dry sliding wear test revealedthat the rheo-processed sample exhibit obviously superior wear resistance because of the microstructure improvement. The dominantmechanism in mild wear condition is abrasion, but it turned to adhesion and oxidation in high applied load and fast sliding velocityconditions.

关键词： rheo-casting aluminum alloy dry wear micro-scratch

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