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When mRNA meets gene editing

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Nano Research 2024年第8期17卷 7337-7356页

作者： Weijie Li Chen Wang yuan lu Key Laboratory of Industrial Biocatalysis Ministry of EducationDepartment of Chemical EngineeringTsinghua UniversityBeijing 100084China Department of Chemical Engineering Tsinghua UniversityBeijing 100084China

The critical challenge of gene therapy lies in delivering gene editing *** with DNA,while RNA is less stable and more accessible to degrade,it comes with the benefit of lower off-target effects since permanent insertion is not *** review focuses on mRNA-based delivery of gene editing agents,highlighting novel mRNA delivery *** provide context,a comparison is made between three main gene editing agents:programmable nucleases,base editors,and prime *** potential of Cas\pi and transposons is also discussed in this ***,a summary of four main barriers to mRNAbased in vivo delivery is ***,this review detailedly introduced different delivery systems,both viral(lentivirus)and non-viral vectors(genome editing via oviductal nucleic acids delivery,lipid nanoparticles,polymer-based nanoparticles,viruslike-particles,extracellular vesicles,and migrasome).Each delivery strategy is assessed by comparing its advantages and disadvantages to offer a comprehensive and objective overview of the delivery ***,we emphasized the vital role of the protein corona as a critical regulator for ***,we concluded the challenges of mRNA-based gene editing strategies(RNA stability,targeting,potential immunogenicity,cytotoxicity,heterogeneity,and rational design).The purpose of this review is to guide further research and provide a comprehensive analysis of mRNA-based in vivo delivery of gene editing agents in this promising field.

关键词： gene editing mRNA delivery viral vectors non-viral vectors CRISPR

Severe liver injury and clinical characteristics of occupational exposure to 2-amino-5-chloro-N,3-dimethylbenzamide: A case series

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Hepatobiliary & Pancreatic Diseases International 2024年第2期23卷 186-194页

作者： Meng-Xiao Feng Hua Zou yuan-Qiang lu Department of Emergency Medicine The First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003China Key Laboratory for Diagnosis and Treatment of Aging and Physic-chemical Injury Diseases of Zhejiang Province Hangzhou 310003China Occupational Health and Radiation Protection Institute Zhejiang Provincial Center for Disease Control and PreventionHangzhou 310051China

Background:The 2-amino-5-chloro-N,3-dimethylbenzamide is a key intermediate in the synthesis of pesticides and ***,no literature currently exists on 2-amino-5-chloro-N,3-dimethylbenzamide poisoning in *** study aimed to reveal the health hazard of this chemical for humans and summarize the clinical characteristics of patients with occupational 2-amino-5-chloro-N,3-dimethylbenzamide ***:This observational study included four patients with 2-amino-5-chloro-N,3-dimethylbenzamide poisoning from June 2022 to July *** entire course of the incidents was described in *** 2-amino-5-chloro-N,3-dimethylbenzamide concentrations were detected by a mass ***-toxylin and eosin staining was performed to assess liver injury,and immunofluorescence was used to evaluate hepatic ***:The 2-amino-5-chloro-N,3-dimethylbenzamide powder(99%purity)entered the human body mainly via the skin and respiratory tract due to poor personal protective *** typical course of 2-amino-5-chloro-N,3-dimethylbenzamide poisoning was divided into latency,rash,fever,organic dam-age,and recovery phases in accordance with the clinical *** and fever may be the important premonitory symptoms for further organ *** chemical was detected in the blood of all patients and caused multiple organ injuries,predominantly liver injury,including kidney,myocardium,and *** patients recovered smoothly after comprehensive treatments,including artificial liver therapy,continuous renal replacement therapy,glucocorticoids,and other symptomatic and supportive *** patient survived by liver *** postoperative pathological findings of the removed liver showed acute liver failure,and immunofluorescence staining confirmed the abundance of mitophagy in residual ***:This study is the first to elaborate the clinical characteristics of patients with 2-amino-5-chloro-N,

关键词： 2-amino-5-chloro-N,3-dimethylbenzamide Poisoning Clinical characteristics Liver injury

Chemico-biological conversion of carbon dioxide

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Journal of Energy Chemistry 2024年第2期89卷 371-387,I0009页

作者： Liangwei Hu Junzhu Yang Qi Xia Jin Zhang Hongxin Zhao yuan lu Zhejiang Province Key Laboratory of Plant Secondary Metabolism and Regulation College of Life Sciences and MedicineZhejiang Sci-Tech UniversityHangzhou 310018ZhejiangChina Key Laboratory of Industrial Biocatalysis Ministry of EducationTsinghua UniversityBeijing 100084China Department of Chemical Engineering Tsinghua UniversityBeijing 100084China College of Biological and Chemical Engineering Jiaxing UniversityJiaxing 314000ZhejiangChina

The unabated carbon dioxide(CO_(2))emission into the atmosphere has exacerbated global climate change,resulting in extreme weather events,biodiversity loss,and an intensified greenhouse *** address these challenges and work toward carbon(C)neutrality and reduced CO_(2)emissions,the capture and utilization of CO_(2)have become imperative in both scientific research and *** cutting-edge approach to achieving efficient catalytic performance involves integrating green bioconversion and chemical *** innovative strategy offers several advantages,including environmental friendliness,high efficiency,and *** study provides a comprehensive review of existing technical routes for carbon sequestration(CS)and introduces two novel CS pathways:the electrochemicalbiological hybrid and artificial photosynthesis *** also thoroughly examines the synthesis of valuable Cnproducts from the two CS systems employing different catalysts and *** both systems heavily rely on electron transfer,direct and mediated electron transfer has been discussed and summarized in ***,this study explores the conditions suitable for different catalysts and assesses the strengths and weaknesses of *** also explored the biocompatibility of the electrode materials and developed novel *** materials were specifically engineered to combine with enzymes or microbial cells to solve the biocompatibility problem,while improving the electron transfer efficiency of ***,this review summarizes the relevant systems developed in recent years for manufacturing different products,along with their respective production efficiencies,providing a solid database for development in this *** novel chemical-biological combination proposed herein holds great promise for the future conversion of CO_(2)into advanced organic ***,it offers exciting prospects for utilizing CO_(2)in synthesizing

关键词： Carbon dioxide Bioelectric synthesis Artificial photosynthesis Synthetic product

Realization of Quantum Secure Direct Communication with Continuous Variable

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Research 2024年第2期6卷 159-170页

作者： Zhengwen Cao yuan lu Geng Chai Hao Yu Kexin Liang Lei Wang School of Information Science and Technology Northwest UniversityXi'an710127China

With the progress of theoretical and applied technologies,the communication system based on the classical encryption is seriously threatened by quantum computing and distributed computing.A communication method that directly loads confidential information on the quantum state,quantum secure direct communication(QSDC),came into being for resisting security ***,we report the first continuous-variable QSDC(CV-QSDC)experimental demonstration for verifying the feasibility and effectiveness of the CV-QSDC protocol based on Gaussian mapping and propose a parameter estimation for signal classification under the actual *** our experiment,we provided 4×10^(2) blocks,where each block contains 105 data for direct information *** the transmission distance of 5 km in our experiment,the excess noise is 0.0035 SNU,where SNU represents the unit of shot-noise *** 4.08×10^(5) bit per second experimental results firmly demonstrated the feasibility of CV-QSDC under the fiber *** proposed grading judgment method based on parameter estimation provides a practical and available message processing scheme for CV-QSDC in a practical fiber channel and lays the groundwork for the grading reconciliation.

关键词： quantum estimation verify

Early Triassic Legoupil Formation in Schmidt Peninsula,Antarctic Peninsula:Provenance and Depositional Settings

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Journal of Earth Science 2024年第2期35卷 317-331页

作者： Chao Zhang Ying-Chun Cui Chen-Guang Liu Fang-Hua Cui lu-yuan Wang Wei-Qiang Zhang School of Resources and Environmental Engineering Shandong University of TechnologyZibo255049China MNR Key Laboratory for Polar Science Polar Research Institute of ChinaShanghai201209China Key Laboratory of Marine Sedimentology and Environmental Geology First Institute of OceanographyState Oceanic AdministrationQingdao266061China Laboratory for Marine Geology Qingdao National Laboratory for Marine Science and TechnologyQingdao266061China Key Laboratory of Mineral Resources Evaluation in Northeast Asia Ministry of Land and ResourcesChangchun130061China

Geochemical compositions can be used to determine the tectonic setting of sedimentary basins,while where the link of source to sink is no longer preserved,detrital zircon age patterns can aid in resolving the original basin *** metasedimentary Legoupil Formation,located at Cape Legoupil and the Schmidt Peninsula,could give a hint for the tectonic evolution of Antarctic *** this contribution,we constrain the sedimentary provenance of the Legoupil Formation through geochemistry and detrital zircon U-Pb *** petrography and geochemical features indicate that the provenance of the Legoupil Formation could be felsic *** zircon grains record a steady supply of Permian and Ordovician material into the Legoupil *** youngest concordant zircon ages of 262 Ma suggest that the depositional time of Legoupil Formation is no older than Late *** detrital zircon age spectrum of Legoupil Formation suggests that the Legoupil Formation sediments should be derived from regional sources endemic to western Gondwana prior to its *** with the previous studies,geochemistry and detrital zircons reflect an active continental margin tectonic setting and the detrital zircon spectra of Legoupil Formation are similar to the ones deposited in forearc tectonic setting.

关键词： Schmidt Peninsula Legoupil Formation Trinity Peninsula Group detrital zircons Antarctic Peninsula geochemistry

Engineering classification recycling of spent lithium-ion batteries through pretreatment:a comprehensive review from laboratory to scale-up application

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Rare Metals 2024年第3期43卷 915-941页

作者： Shu-Xuan Yan You-Zhou Jiang Xiang-Ping Chen lu yuan Ting-Ting Min Yu Cao Wan-Li Peng Tao Zhou Hunan Provincial Key Laboratory of Chemical Power Sources College of Chemistry and Chemical EngineeringCentral South UniversityChangsha 410083China College of Chemistry and Chemical Engineering Hunan Normal UniversityChangsha 410083China School of Public Policy and Management Tsinghua UniversityBeijing 100084China China Nonferrous Metal Industry's Foreign Engineering and Construction Co. LtdBeijing 100029China

The lithium-ion batteries(LIBs)have been widely equipped in electric/hybrid electric vehicles(EVs/HEVs)and the portable electronics due to their excellent electrochemical ***,a large number of retired LIBs that consist of toxic substances(e.g.,heavy metals,electrolytes)and valuable metals(e.g.,Li,Co)will inevitably flow into the waste stream,and their incineration or landfill treatment will cause severe risks to ecosystem and human *** sustainable and efficient treatment or recycling of valuable resources from spent LIBs should be fully recognized for environmental and resource *** one of the most important processes for spent LIBs recycling,the pretreatment is an indispensable step,which is directly related to the subsequent metal extraction and separation *** considerable progresses have been made regarding the pretreatment technologies,there are few summarized reports concerning critical processes of spent LIBs recycling,especially combination of currently available recycling technologies with industrialized applications during ***,comprehensive review of the current prevailing pretreatment technologies in laboratory to existing scale-up applications is quite necessary to reveal cutting-edge development in the field of *** this review,the current pretreatment technologies are systematically categorized and introduced,along with critical *** review focused on the various options for pretreatment processes itself,instead of general spent LIBs recycling technologies without the focused topics that have been sophisticatedly reviewed by previous ***,the deactivation,discharge,dismantling,separation,liberation of active material and electrolyte treatment have been summarized with the in-depth discussion of the technology development and current status of each ***,current states of industrial development are also reviewed and discussed for the development of

关键词： Spent lithium-ion batteries(LIBs) Recycling Pretreatment Valuable metals Cutting-edge technologies Industrialized application

Neferine inhibits the progression of diabetic nephropathy by modulating the miR-17-5p/nuclear factor E2-related factor 2 axis

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Journal of Traditional Chinese Medicine 2024年第1期44卷 44-53页

作者： HUANG Hongmei YANG Maojun LI Ting WANG Dandan LI Ying TANG Xiaochi yuan lu GU Shi XU Yong Department of Endocrinology Chengdu Shuangliu District First People's Hospital(West China Airport of Sichuan University)Chengdu 610200China Department of Endocrinology Affiliated Hospital of Southwest Medical UniversityLuzhou 646000China

OBJECTIVE:To investigate the effect of Neferine(Nef)on diabetic nephropathy(DN)and to explore the mechanism of Nef in DN based on miRNA regulation ***:A DN mouse model was constructed and treated with *** creatinine(Crea),blood urea(UREA)and urinary albumin were measured in mice by kits,and renal histopathological changes and fibrosis were observed by hematoxylin-eosin staining and Masson *** tissue superoxide dismutase(SOD),malondialdehyde(MDA)and glutathione peroxidase(GSH-Px)activities were measured by enzyme-linked immunosorbent assay(ELISA).Western blotting was used to detect the expression of nuclear factor E2-related factor 2(Nrf2)/heme oxygenase 1(HO-1)signaling pathway-related proteins in kidney *** reverse transcription-polymerase chain reaction(q RT-PCR)was used to detect the expression of miR-17-5p in kidney ***,a DN in vitro model was constructed by high glucose culture of human mesangial cells(HMCs),cells were transfected with miR-17-5p mimic and/or treated with Nef,and we used q RTPCR to detect cellular miR-17 expression,flow cytometry to detect apoptosis,ELISAs to detect cellular SOD,MDA,and GSH-Px activities,Western blots to detect Nrf2/HO-1 signaling pathway-related protein expression,and dual luciferase reporter gene assays to verify the targeting relationship between Nrf2 and ***:Administration of Nef significantly reduced the levels of blood glucose,Crea,and UREA and the expression of miR-17-5p,improved renal histopathology and fibrosis,significantly reduced MDA levels,elevated SOD and GSH-Px activities,and activated Nrf2 expression in kidney tissues from mice with ***2 is a post-transcriptional target of *** HMCs transfected with miR-17-5p mimics,the m RNA and protein levels of Nrf2 were significantly ***,miR-17-5p overexpression and Nef intervention resulted in a significant increase in high glucose-induced apoptosis and MDA levels in HMC

关键词： diabetic nephropathies neferine miR-17-5p NF-E2-related factor 2 oxidative stress

Implications for fault reactivation and seismicity induced by hydraulic fracturing

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Petroleum Science 2024年第2期21卷 1081-1098页

作者： Zi-Han Sun Ming-Guang Che Li-Hong Zhu Shu-Juan Zhang Ji-yuan lu Chang-Yu Jin Key Laboratory of Ministry of Education on Safe Mining of Deep Metal Mines School of Resources and Civil Engineering and Key Laboratory of Liaoning Province on Deep Engineering and Intelligent TechnologyNortheastern UniversityShenyang110819LiaoningChina Research Institute of Petroleum Exploration&Development PetroChina Company LimitedBeijing100084China The Exploration and Development Research Institute of Daqing Oilfield Limited Company Daqing163458HeilongjiangChina

Evaluating the physical mechanisms that link hydraulic fracturing(HF) operations to induced earthquakes and the anticipated form of the resulting events is significant in informing subsurface fluid injection operations. Current understanding supports the overriding role of the effective stress magnitude in triggering earthquakes, while the impact of change rate of effective stress has not been systematically addressed. In this work, a modified critical stiffness was brought up to investigate the likelihood, impact,and mitigation of induced seismicity during and after hydraulic fracturing by developing a poroelastic model based on rate-and-state fraction law and linear stability analysis. In the new criterion, the change rate of effective stress was considered a key variable to explore the evolution of this criterion and hence the likelihood of instability slip of fault. A coupled fluid flow-deformation model was used to represent the entire hydraulic fracturing process in COMSOL Multiphysics. The possibility of triggering an earthquake throughout the entire hydraulic fracturing process, from fracturing to cessation, was investigated considering different fault locations, orientations, and positions along the fault. The competition between the effects of the magnitude and change rate of effective stress was notable at each fracturing stage. The effective stress magnitude is a significant controlling factor during fracturing events, with the change rate dominating when fracturing is suddenly started or stopped. Instability dominates when the magnitude of the effective stress increases(constant injection at each fracturing stage) and the change rate of effective stress decreases(the injection process is suddenly stopped). Fracturing with a high injection rate, a fault adjacent to the hydraulic fracturing location and the position of the junction between the reservoir and fault are important to reduce the Coulomb failure stress(CFS) and enhance the critical stiffness as

关键词： Hydraulic fracturing Coulomb failure stress Rate-and-state fraction model Linear stability analysis Critical stiffness Seismically induced fault

Characterization of distinct microbiota associated with androgenetic alopecia patients treated and untreated with platelet-rich plasma(PRP)

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Animal Models and Experimental Medicine 2024年第2期7卷 106-113页

作者： Qian Zhang Yanan Wang Cheng Ran Yingmei Zhou Zigang Zhao Tianhua Xu Hongwei Hou yuan lu Beijing Life Science Academy BeijingChina Department of Pathology Affiliated Hospital of Hebei UniversityBaodingChina Department of Otolaryngology Affiliated Hospital of Hebei UniversityBaodingChina Department of Dermatology Huazhong University of Science and Technology Union Shenzhen HospitalShenzhenChina Department of Dermatology Hainan Hospital of PLA General HospitalSanyaChina Department of Chemical Engineering Tsinghua UniversityBeijingChina

Background:Androgenic alopecia(AGA)is the most common type of hair loss in men,and there are many studies on the treatment of hair loss by platelet-rich plasma(PRP).The human scalp contains a huge microbiome,but its role in the process of hair loss remains unclear,and the relationship between PRP and the microbiome needs further ***,the purpose of this study was to investigate the effect of PRP treatment on scalp microbiota ***:We performed PRP treatment on 14 patients with AGA,observed their clinical efficacy,and collected scalp swab samples before and after *** scalp microflora of AGA patients before and after treatment was characterized by amplifying the V3-V4 region of the 16 s RNA gene and sequencing for bacterial ***:The results showed that PRP was effective in the treatment of AGA patients,and the hair growth increased *** results of relative abundance analysis of microbiota showed that after treatment,g_Cutibacterium increased and g_Staphylococcus decreased,which played a stable role in scalp *** addition,g_Lawsonella decreased,indicating that the scalp oil production decreased after ***:The findings suggest that PRP may play a role in treating AGA through scalp microbiome rebalancing.

关键词： androgenic alopecia microbiome platelet-rich plasma scalp