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A Global Minimization Algorithm for Empirical Contact Potential Functions

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Chemical Research in Chinese Universities 2006年第2期22卷 248-250页

作者： WANG yu-hong li Wei Department of Molecular Biology College of Life Sciences Jilin University Changchun 130021 P. R. China

Global minimization algorithm is indispensable for solving protein folding problems based on thermodynamic hypothesis. A contact difference （CD） based on pseudo potential function, for simulating empirical contact potential functions and testing global minimization algorithm was proposed. The present article describes a conformational sampiing and global minimization algorithm, which is called WL, based on Monte Carlo simulation and simulated annealing. It can be used to locate CD＇s globe minimum and refold extended protein structures, as small as 0. 03 nm, from the native structures, back to ones with root mean square distance（RMSD）. These results demonstrate that the global minimization problems for empirical contact potential functions may be solvable.

关键词： Global minimization Contact potential function Simulated annealing Monte Carlo simulation

Molecular Biological and Biochemical Studies Reveal New Pathways Important for Cotton Fiber Development

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Journal of Integrative Plant Biology 2007年第1期49卷 69-74页

作者： yu Xu hong-Bin li yu-Xian Zhu National Laboratory of Protein Engineering and Plant Genetic Engineering Peking University Beijing 100871 China

As one of the longest single-celled seed trichomes, fibers provide an excellent model for studying fundamental biological processes such as cell differentiation, cell expansion, and cell wall biosynthesis. In this review, we summarize recent progress in cotton functional genomic studies that characterize the dynamic changes in the transcriptomes of fiber cells. Extensive expression profilings of cotton fiber transcriptomes have provided comprehensive information, as quite a number of transcription factors and enzyme-coding genes have been shown to express preferentially during the fiber elongation period. Biosynthesis of the plant hormone ethylene is found significantly upregulated during the fiber growth period as revealed by both microarray analysis and by biochemical and physiological studies. It is suggested that genetic engineering of the ethylene pathway may improve the quality and the productivity of cotton lint. Many metabolic pathways, such as biosynthesis of cellulose and matrix polysaccharides are preferentially expressed in actively growing fiber cells. Five gene families, including proline-rich proteins （PRP）, arabinogalactan proteins （AGP）, expansins, tubulins and lipid transfer proteins （LTP） are activated during early fiber development, indicating that they may also be needed for cell elongation. In conclusion, we identify a few areas of future research for cotton functional genomic studies.

关键词： cotton fiber functional genomics plant hormone transcriptome transcription factors

INVERSE OPAliNE STRUCTURE TITANIA PHOTONIC CRYSTALS BY FUNCTIONALLY MODIFIED COLLOIDAL CRYSTALS TEMPLATING

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Chinese Journal of Polymer Science 2007年第2期25卷 217-220页

作者： hong-yu li Zhi-qiang Chen Hai-qiao Wang Bo Meng Xiao-yu li Department of Chemistry Anyang Teachers College Anyang Henan 455000 China The Key Laboratory of Nanomaterials of Ministry of Education College of Materials Science and Engineering Beijing University of Chemical Technology Beijing 100029 China

Ordered macroporous titania photonic crystals （PCs） and photonic balls were fabricated by functional modified polymer colloidal crystals. The TiO2 PCs and balls formed through this method exhibit no cracks and lacun... 详细信息

关键词： Photonic crystals Photonic balls Ordered macroporous titania Polymer colloidal crystals.

Postoperative efficacy,safety,predictability,and visual quality of implantable collamer lens implantation versus small incision lenticule extraction in myopic eyes:a Meta-analysis

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International Journal of Ophthalmology(English edition) 2023年第3期16卷 442-452页

作者： hong-yu li Zi Ye Zhao-Hui li Medical School of Chinese PLA Beijing 100853China Senior Department of Ophthalmology the Third Medical Center of PLA General HospitalBeijing 100853China

AIM:To compare the postoperative efficacy,safety,predictability,and visual quality of implantable collamer lens(ICL)implantation versus small incision lenticule extraction(SMILE)in myopia ***:Pub Med,EMBASE,Web of Science,Cochrane library and several Chinese databases were searched at May 2021 to select relevant studies in comparison of clinical outcomes between ICL implantation and SMILE for *** primary outcomes were efficacy,safety,and *** the secondary outcomes were postoperative higher-order ocular aberrations(HOAs),modulation transfer function cutoff frequency(MTF),objective scatter index(OSI),contrast sensitivity and a quality of vision(Qo V)***:A total of 1036 eyes from 10 studies,of which 503 eyes underwent ICL implantation and 533 eyes underwent SMILE,were enrolled in this *** results revealed that ICL group had a better safety index and post-corrected distance visual acuity(CDVA)(P=0.007,line(P=0.007)than the SMILE *** significant differences were found in comparison of the other primary *** the longterm follow-up(>6mo),ICL group had a lower total HOA,coma,and spherical aberration than SMILE group(P=0.003,lity for correcting high ***,ICL group is relatively safer and also has better visual quality in comparison of SMILE group.

关键词： implantable collamer lens small incision lenticule extraction myopia refractive surgery Meta-analysis

Salvage liver transplantation in the treatment of hepatocellular carcinoma:A Meta-analysis

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World Journal of Gastroenterology 2012年第19期18卷 2415-2422页

作者： hong-yu li Yong-Gang Wei Lv-Nan Yan Bo li Department of liver and Vascular Surgery Center of Liver TransplantationWest China HospitalSichuan UniversityChengdu 610041Si-chuan ProvinceChina

AIM:To evaluate survival and recurrence after salvage liver transplantation(SLT) for the treatment of hepatocellular carcinoma(HCC) compared with primary liver transplantation(PLT) using a ***:literature on SLT versus PLT for the treatment of HCC published between 1966 and July 2011 was retrieved.A meta-analysis was conducted to estimate pooled survival and disease-free rates.A fixed or random-effect model was established to collect the ***:The differences in overall survival and disease-free survival rates at 1-year,3-year and 5-year survival rates were not statistically significant between SLT group and PLT group(P > 0.05).After stratifying the various studies by donor source and Milan criteria,we found that:(1) living donor liver transplantation recipients had significantly higher 1-year survival rate,lower 3-year and 5-year survival rates compared with deceased-donor liver transplantation(DDLT) *** in DDLT recipients they had better 1-year and 5-year disease-free survival rate in SLT group;and(2) No difference was seen in 1-year,3-year and 5-year survival rates between two groups who beyond Milan criteria at the time of liver ***:SLT can be effectively performed for patients with recurrence or deterioration of liver function after hepatectomy for *** does not increase the perioperative mortality and has a similar long-term survival rates compared to PLT.

关键词： Salvage liver transplantation Primary livertransplantation Hepatocellular carcinoma Meta-analy-sis Survival rate

Hydrogen Peroxide in Plants:a Versatile Molecule of the Reactive Oxygen Species Network

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Journal of Integrative Plant Biology 2008年第1期50卷 2-18页

作者： li-Juan Quan Bo Zhang Wei-Wei Shi hong-yu li MOE Key Laboratory of Arid and Grassland Ecology School of Life Sciences Lanzhou University Lanzhou 730000 China

Plants often face the challenge of severe environmental conditions, which include various biotic and abiotic stresses that exert adverse effects on plant growth and development. During evolution, plants have evolved complex regulatory mechanisms to adapt to various environmental stressors. One of the consequences of stress is an increase in the cellular concentration of reactive oxygen species （ROS）, which are subsequently converted to hydrogen peroxide （H2O2）. Even under normal conditions, higher plants produce ROS during metabolic processes. Excess concentrations of ROS result in oxidative damage to or the apoptotic death of cells. Development of an antioxidant defense system in plants protects them against oxidative stress damage. These ROS and, more particularly, H2O2, play versatile roles in normal plant physiological processes and in resistance to stresses. Recently, H2O2 has been regarded as a signaling molecule and regulator of the expression of some genes in cells. This review describes various aspects of H2O2 function, generation and scavenging, gene regulation and cross-links with other physiological molecules during plant growth, development and resistance responses.

关键词： antioxidant system gene regulation hydrogen peroxide (H2O2) reactive oxygen species (ROS) signaling molecule.

Two new flavonoid diglycosides from Cephalotaxus sinensis

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Chinese Chemical Letters 2007年第7期18卷 837-839页

作者： Wei li Rong Ji Dai yu hong yu liang li Wei Wei Meng yu lin Deng School of life Science and Technology Beijing Institute of Technology Beijing 100081 China

Two new acetylated flavonoid diglycosides, scutellarein-5-O-[α-L-rhamnopyranosyl-（1 → 4）-β-D-6-O-acetylglucopyranoside]（1）, apigenin-5-O-[α-L-rhamnopyranosyl-（1→ 2）-β-D-3,6-di-O-acetylglucopyranoside] （2）, were isolated from the leaves of Cephalotaxus sinensis. Their structures were determined by chemical and spectroscopic methods including HR-FAB-MS,^1H-^1H COSY, HSQC and HMBC experiments.

关键词： Cephalotaxus sinensis (Rehd et Wile) li Flavonoid diglycosides Scutellarein-5-O-[α-L-rhamnopyranosyl-(1→ 4)-β-D-6-O-acetylglucopyranoside] Apigenin-5-O-[α-L-rhamnopyranosyl-(1 → 2)-β-D-3,6-di-O-acetylglucopyranoside]

Microsatellite Variation in the Oyster Crassostrea plicatula Along the Coast of China

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Journal of Ocean University of China 2008年第4期7卷 432-438页

作者： yu hong, li Qi yu Ruihai College of Fisheries Ocean University of China Qingdao 266003 P. R. China

Genetic diversity and differentiation of the oyster Crassostrea plicatula populations from China’s coast were studied based on seven microsatellite loci. All loci showed high polymorphism for all five C. plicatula populations,with an average number of allele per locus of 19.3-27.9 and an average expected heterozygosity of 0.889-0.952. Significant departures from Hardy-Weinberg equilib-rium and deficits of heterozygotes were observed over most populations at each locus,which were fully explained by null alleles. Microsatellite analysis revealed significant subdivision in the C. plicatula populations. According to the neighbor-joining tree con-structed on the basis of the DA distance,the five populations fell into three regional groups,showing a relatively homogeneous genetic structure in geographically close populations. Assignation tests correctly assigned high percentages of individuals to their original populations and groups,and also confirmed the existence of genetic differentiation among C. plicatula populations. The results ob-tained in this study will facilitate the formulation of appropriate fisheries management programs,stock identification and conservation of biodiversity for the species.

关键词： Crassostrea plicatula microsatellites genetic diversity population structure

KAI1 reverses the epithelial-mesenchymal transition in human pancreatic cancer cells

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Hepatobiliary & Pancreatic Diseases International 2019年第5期18卷 471-477页

作者： Xu liu Xiao-Zhong Guo hong-yu li Jiang Chen Department of Gastroenterology General Hospital of Shenyang Military Area

Background: Epithelial-mesenchymal transition (EMT) plays an important role in pancreatic cancer (PC). In the present study, we investigated the effects of KAI1 gene overexpression on the EMT of human PC cell lines, MIA PaCa-2 and PACN-1. Methods: Plasmids overexpressing KAI1 and pCMV were transfected into MIA PaCa-2 and PACN-1 cells, respectively. After selection of differently transfected cells by G418, KAI1 protein levels were examined by Western blotting, and transfected cells were renamed as MIA PaCa-2-K, MIA PaCa-2-p, PACN-1-K and PACN-1-p. Wound healing and Transwell migration assays were then performed comparing the two groups of cells. EMT-related markers were analyzed by Western blotting. Results: The percentage of wound closure significantly decreased in MIA PaCa-2-K cells compared with MIA PaCa-2-p and MIA PaCa-2 cells after 24, 48 and 72 h ( P < 0.05). In PACN-1-K cells, the percentage of wound closure significantly decreased as well ( P < 0.05). Numbers of invading MIA PaCa-2, MIA PaCa-2-p and MIA PaCa-2-K cells were determined as 48.0 ±15.4, 50.0 ±12.4, and 12.0 ±3.8, respectively. The corresponding numbers of invading PACN-1, PACN-1-p and PACN-1-K cells were 29.0 ±10.6, 31.0 ±11.4, and 8.0 ±4.2, respectively. KAI1 overexpression induced a significant upregulation of E-cadherin and also significant downregulation of Snail, vimentin, matrix metalloproteinase 2 (MMP2) and MMP9 (all P < 0.05) in PC cells. Conclusions: KAI1 reversed EMT-related marker expression and inhibited migration and invasion of PC cells. Thus, KAI1 might represent a novel potential therapeutic target for PC.

关键词： KAI1 Epithelial-mesenchymal transition Pancreatic cancer